/ A. Gittel [et al.] // ISME J. - 2013, DOI 10.1038/ismej.2013.219
. - ISSN 1751-7362
Аннотация: Cryoturbation, the burial of topsoil material into deeper soil horizons by repeated freeze-thaw events, is an important storage mechanism for soil organic matter (SOM) in permafrost-affected soils. Besides abiotic conditions, microbial community structure and the accessibility of SOM to the decomposer community are hypothesized to control SOM decomposition and thus have a crucial role in SOM accumulation in buried soils. We surveyed the microbial community structure in cryoturbated soils from nine soil profiles in the northeastern Siberian tundra using high-throughput sequencing and quantification of bacterial, archaeal and fungal marker genes. We found that bacterial abundances in buried topsoils were as high as in unburied topsoils. In contrast, fungal abundances decreased with depth and were significantly lower in buried than in unburied topsoils resulting in remarkably low fungal to bacterial ratios in buried topsoils. Fungal community profiling revealed an associated decrease in presumably ectomycorrhizal (ECM) fungi. The abiotic conditions (low to subzero temperatures, anoxia) and the reduced abundance of fungi likely provide a niche for bacterial, facultative anaerobic decomposers of SOM such as members of the Actinobacteria, which were found in significantly higher relative abundances in buried than in unburied topsoils. Our study expands the knowledge on the microbial community structure in soils of Northern latitude permafrost regions, and attributes the delayed decomposition of SOM in buried soils to specific microbial taxa, and particularly to a decrease in abundance and activity of ECM fungi, and to the extent to which bacterial decomposers are able to act as their functional substitutes.The ISME Journal advance online publication, 12 December 2013; doi:10.1038/ismej.2013.219.
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Держатели документа:
1] Department of Biology, Centre for Geobiology, University of Bergen, Bergen, Norway
Austrian Polar Research Institute, Vienna, Austria
Department of Ecosystems Biology, University of South Bohemia, Ceske Budejovice, Czech Republic
Institut fur Bodenkunde, Leibniz Universitat Hannover, Hannover, Germany
1] Institute of Genomics and Systems Biology, Argonne National Laboratory, Argonne, IL, USA
Computation Institute, University of Chicago, Chicago, IL, USA
Department of Ecology and Evolution, University of Chicago, Chicago, IL, USA
1] Austrian Polar Research Institute, Vienna, Austria
Division of Terrestrial Ecosystem Research, Department of Microbiology and Ecosystem Science, University of Vienna, Vienna, Austria
Department of Earth Science, Centre for Geobiology, University of Bergen, Bergen, Norway
Division of Ecosystem Modelling, Institute of Coastal Research, Helmholtz Zentrum Geesthacht, Geesthacht, Germany
Central Siberian Botanical Garden, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia
1] Institut fur Bodenkunde, Leibniz Universitat Hannover, Hannover, Germany
VN Sukachev Institute of Forest, Siberian Branch of Russian Academy of Sciences, Akademgorodok, Russia
Department of Biology, Centre for Geobiology, University of Bergen, Bergen, Norway
Division of Archaea Biology and Ecogenomics, Department of Ecogenomics and Systems Biology, University of Vienna, Vienna, Austria
Доп.точки доступа:
Gittel, A.; Barta, J.; Kohoutova, I.; Mikutta, R.; Owens, S.; Gilbert, J.; Schnecker, J.; Wild, B.; Hannisdal, B.; Maerz, J.; Lashchinskiy, N.; Capek, P.; Santruckova, H.; Gentsch, N.; Shibistova, O.; Guggenberger, G.; Richter, A.; Torsvik, V.L.; Schleper, C.; Urich, T.
Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН
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Найдено документов в текущей БД: 7
Biological activity assessment of museum cultures of antagonist microorganisms and their use for presowing treatment of Scots pine seeds (Pinus sylvestris L.) in vitro
/ O. E. Kondakova, I. D. Grodnitskaya // Vestn. Tomsk. Gos. Univ. Biol. - 2018. - Is. 42. - С. 54-68, DOI 10.17223/19988591/42/3
. - ISSN 1998-8591
Кл.слова (ненормированные):
Antagonistic and enzymatic activity -- Bacillus -- Growthpromoting effect -- Phytopathogens -- Streptomyces -- Trichoderma
Аннотация: The microbiological method is applied for the purpose of artificial forest regeneration, as the most effective method of protecting forest planting material grown in forest nurseries. At present, literature data contain many examples of using species and genera of microorganisms belonging to different taxa in order to protect plants. The aim of the research was to establish biological (antagonistic, enzymatic and growth-stimulating) activity of the museum microorganism cultures belonging to different taxonomic groups (bacteria, fungi), and to assess their influence on the growth and development of Scots pine seeds in vitro and a decrease in the number of phytopathogenic fungi. We isolated previously selected microorganisms from the nursery soils; these microorganisms belong to different taxonomic groups, namely, Trichoderma micromycetes (T. harzianum, T. longibrachaitum, and T. lignorum), Streptomyces lateritius bacteria, Bacillus amyloliquefaciens, as well as phytopathogenic Fusarium fungi (F. oxysporum, F. moniliforme, F. proliferatum, F. moniliforme var annullatum, and F. oxysporum B3). Antagonistic activity of microbial strains was determined by the dual culture method, and the presence of enzymatic activity (lipase, proteinase and chitinase) of the tested strains was observed by qualitative express tests. We studied the growth-promoting activity by soaking pine seeds in aqueous suspensions of antagonists (106 spores/ml) (Pegalado, 2000; Cullimore, 2001; Montealegre, 2003; Asaturova, 2012). The results of the research showed that the investigated microorganisms (fungi, actinobacteria and bacteria) are biologically active. The most powerful antagonists were micromycetes of T. harzianum, T. lignorum, and T. longibrachiatum, which are also capable of exhibiting mycophilic properties (hyperparasitism). Thus, T. longibrachiatum showed mycophilia against three strains: F. moniliforme, F. moniliforme var annulatum, and F. oxysporum B3, whereas T. harzianum and T. lignorum did against two: F. moniliforme and F. proliferatum; the degree of phytopathogen inhibition (ID) varied from 30 to 100% (See Table 1). The strain of B. amiloliquefaciens bacterium was less active, the DI was 41.4%, on the average, and the slowest antagonistic properties were exhibited by actinobacterium S. lateritius - 14.8%, on the average. The investigation of the presence of the main hydrolytic enzymes (a hitinaze, a lipase, protease) showed that Trichoderma micromycetes had the average and strong hydrolytic activity (T. harzianum and T. longibrachiatum), and bacteria (S. lateritius, B. amyloliquefaciens) had the average and weak hydrolytic activity (See Table 2). Also, all the investigated strains improved Scots pine seed germination, while the strains of B. amyloliquefaciens and T. longibrachiatum showed the greatest growth-promoting activity (See Figures). Thus, we found that the investigated strains (T. harzianum, T. lignorum, T. longibrachiatum, S. lateritius, and B. amyloliquefaciens) had a high antagonistic activity, and Trichoderma micromycetes revealed the ability for mycoparasitism. The high biological (enzymatic, antagonistic, growth-stimulating) activity of the studied strains of microorganisms makes them effective agents for biological control in forest nurseries. © 2018 Tomsk State University. All rights reserved.
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Держатели документа:
Laboratory of Microbiology and Ecological Biotechnology, VN Sukachev Institute of Forest, Siberian Branch of the Russian Academy of Sciences, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Kondakova, O. E.; Grodnitskaya, I. D.
Кл.слова (ненормированные):
Antagonistic and enzymatic activity -- Bacillus -- Growthpromoting effect -- Phytopathogens -- Streptomyces -- Trichoderma
Аннотация: The microbiological method is applied for the purpose of artificial forest regeneration, as the most effective method of protecting forest planting material grown in forest nurseries. At present, literature data contain many examples of using species and genera of microorganisms belonging to different taxa in order to protect plants. The aim of the research was to establish biological (antagonistic, enzymatic and growth-stimulating) activity of the museum microorganism cultures belonging to different taxonomic groups (bacteria, fungi), and to assess their influence on the growth and development of Scots pine seeds in vitro and a decrease in the number of phytopathogenic fungi. We isolated previously selected microorganisms from the nursery soils; these microorganisms belong to different taxonomic groups, namely, Trichoderma micromycetes (T. harzianum, T. longibrachaitum, and T. lignorum), Streptomyces lateritius bacteria, Bacillus amyloliquefaciens, as well as phytopathogenic Fusarium fungi (F. oxysporum, F. moniliforme, F. proliferatum, F. moniliforme var annullatum, and F. oxysporum B3). Antagonistic activity of microbial strains was determined by the dual culture method, and the presence of enzymatic activity (lipase, proteinase and chitinase) of the tested strains was observed by qualitative express tests. We studied the growth-promoting activity by soaking pine seeds in aqueous suspensions of antagonists (106 spores/ml) (Pegalado, 2000; Cullimore, 2001; Montealegre, 2003; Asaturova, 2012). The results of the research showed that the investigated microorganisms (fungi, actinobacteria and bacteria) are biologically active. The most powerful antagonists were micromycetes of T. harzianum, T. lignorum, and T. longibrachiatum, which are also capable of exhibiting mycophilic properties (hyperparasitism). Thus, T. longibrachiatum showed mycophilia against three strains: F. moniliforme, F. moniliforme var annulatum, and F. oxysporum B3, whereas T. harzianum and T. lignorum did against two: F. moniliforme and F. proliferatum; the degree of phytopathogen inhibition (ID) varied from 30 to 100% (See Table 1). The strain of B. amiloliquefaciens bacterium was less active, the DI was 41.4%, on the average, and the slowest antagonistic properties were exhibited by actinobacterium S. lateritius - 14.8%, on the average. The investigation of the presence of the main hydrolytic enzymes (a hitinaze, a lipase, protease) showed that Trichoderma micromycetes had the average and strong hydrolytic activity (T. harzianum and T. longibrachiatum), and bacteria (S. lateritius, B. amyloliquefaciens) had the average and weak hydrolytic activity (See Table 2). Also, all the investigated strains improved Scots pine seed germination, while the strains of B. amyloliquefaciens and T. longibrachiatum showed the greatest growth-promoting activity (See Figures). Thus, we found that the investigated strains (T. harzianum, T. lignorum, T. longibrachiatum, S. lateritius, and B. amyloliquefaciens) had a high antagonistic activity, and Trichoderma micromycetes revealed the ability for mycoparasitism. The high biological (enzymatic, antagonistic, growth-stimulating) activity of the studied strains of microorganisms makes them effective agents for biological control in forest nurseries. © 2018 Tomsk State University. All rights reserved.
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Держатели документа:
Laboratory of Microbiology and Ecological Biotechnology, VN Sukachev Institute of Forest, Siberian Branch of the Russian Academy of Sciences, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Kondakova, O. E.; Grodnitskaya, I. D.
Carbon and nitrogen recycling from microbial necromass to cope with C:N stoichiometric imbalance by priming
/ J. Cui, Z. Zhu, X. Xu [et al.] // Soil Biol. Biochem. - 2020. - Vol. 142. - Ст. 107720, DOI 10.1016/j.soilbio.2020.107720
. - ISSN 0038-0717
Кл.слова (ненормированные):
Compound-specific 13C-PLFA analysis -- Glucose mineralization -- Necromass recycling -- Priming effects -- Soil carbon -- Stoichiometric imbalance -- Carbon dioxide -- Glucose -- Mineralogy -- Nitrogen -- Recycling -- Soils -- Compound-specific. 3C-PLFA analysis -- Necromass -- Priming effects -- Soil carbon -- Stoichiometric imbalance -- Bacteria -- anoxic conditions -- biomineralization -- carbon isotope -- decomposition -- glucose -- nitrogen -- nutrient cycling -- soil carbon -- soil nitrogen -- soil organic matter -- stoichiometry -- Actinobacteria (class) -- Bacteria (microorganisms) -- Fungi -- Negibacteria -- Posibacteria
Аннотация: The impact of increasing amounts of labile C input on priming effects (PE) on soil organic matter (SOM) mineralization remains unclear, particularly under anoxic conditions and under high C input common in microbial hotspots. PE and their mechanisms were investigated by a 60-day incubation of three flooded paddy soils amended with13C-labeled glucose equivalent to 50–500% of microbial biomass C (MBC). PE (14–55% of unamended soil) peaked at moderate glucose addition rates (i.e., 50–300% of MBC). Glucose addition above 300% of MBC suppressed SOM mineralization but intensified microbial N acquisition, which contradicted the common PE mechanism of accelerating SOM decomposition for N-supply (frequently termed as “N mining”). Particularly at glucose input rate higher than 3 g kg?1 (i.e., 300–500% of MBC), mineral N content dropped on day 2 close to zero (1.1–2.5 mg N kg?1) because of microbial N immobilization. To cope with the N limitation, microorganisms greatly increased N-acetyl glucosaminidase and leucine aminopeptidase activities, while SOM decomposition decreased. Several discrete peaks of glucose-derived CO2 (contributing >80% to total CO2) were observed between days 13–30 under high glucose input (300–500% of MBC), concurrently with CH4 peaks. Such CO2 dynamics was distinct from the common exponential decay pattern, implicating the recycling and mineralization of 13C-enriched microbial necromass driven by glucose addition. Therefore, N recycling from necromass was hypothesized as a major mechanism to alleviate microbial N deficiency without SOM priming under excess labile C input. Compound-specific 13C-PLFA confirmed the redistribution of glucose-derived C among microbial groups, i.e., necromass recycling. Following glucose input, more than 4/5 of total 13C-PLFA was in the gram-negative and some non-specific bacteria, suggesting these microorganisms as r-strategists capable of rapidly utilizing the most labile C. However, their 13C-PLFA content decreased by 70% after 60 days, probably as a result of death of these r-strategists. On the contrary, the 13C-PLFA in gram-positive bacteria, actinomycetes and fungi (K-strategists) was initially minimal but increased by 0.5–5 folds between days 2 and 60. Consequently, the necromass of dead r-strategists provided a high-quality C–N source to the K-strategists. We conclude that under severe C excess, N recycling from necromass is a much more efficient microbial strategy to cover the acute N demand than N acquisition from the recalcitrant SOM. © 2020 Elsevier Ltd
Scopus
Держатели документа:
Key Laboratory of Agro-ecological Processes in Subtropical Region & Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Gongdong, 510640, China
Jiangsu Provincial Key Laboratory for Bioresources of Coastal Saline Soils, Jiangsu Coastal Biological Agriculture Synthetic Innovation Center, Yancheng Teachers' University, Yancheng, 224002, China
Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China
School of Environment, Natural Resources & Geography, Bangor University, Gwynedd, LL57 2UW, United Kingdom
Department of Agricultural Soil Science, Department of Soil Science of Temperate Ecosystems, University of G?ttingen, G?ttingen, Germany
Institute of Environmental Sciences, Kazan Federal University, Kazan, 420049, Russian Federation
Agro-Technological Institute, RUDN University, Moscow, 117198, Russian Federation
Departamento de Ciencias Quimicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile
VN Sukachev Institute of Forest, SB-RAS, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Cui, J.; Zhu, Z.; Xu, X.; Liu, S.; Jones, D. L.; Kuzyakov, Y.; Shibistova, O.; Wu, J.; Ge, T.
Кл.слова (ненормированные):
Compound-specific 13C-PLFA analysis -- Glucose mineralization -- Necromass recycling -- Priming effects -- Soil carbon -- Stoichiometric imbalance -- Carbon dioxide -- Glucose -- Mineralogy -- Nitrogen -- Recycling -- Soils -- Compound-specific. 3C-PLFA analysis -- Necromass -- Priming effects -- Soil carbon -- Stoichiometric imbalance -- Bacteria -- anoxic conditions -- biomineralization -- carbon isotope -- decomposition -- glucose -- nitrogen -- nutrient cycling -- soil carbon -- soil nitrogen -- soil organic matter -- stoichiometry -- Actinobacteria (class) -- Bacteria (microorganisms) -- Fungi -- Negibacteria -- Posibacteria
Аннотация: The impact of increasing amounts of labile C input on priming effects (PE) on soil organic matter (SOM) mineralization remains unclear, particularly under anoxic conditions and under high C input common in microbial hotspots. PE and their mechanisms were investigated by a 60-day incubation of three flooded paddy soils amended with13C-labeled glucose equivalent to 50–500% of microbial biomass C (MBC). PE (14–55% of unamended soil) peaked at moderate glucose addition rates (i.e., 50–300% of MBC). Glucose addition above 300% of MBC suppressed SOM mineralization but intensified microbial N acquisition, which contradicted the common PE mechanism of accelerating SOM decomposition for N-supply (frequently termed as “N mining”). Particularly at glucose input rate higher than 3 g kg?1 (i.e., 300–500% of MBC), mineral N content dropped on day 2 close to zero (1.1–2.5 mg N kg?1) because of microbial N immobilization. To cope with the N limitation, microorganisms greatly increased N-acetyl glucosaminidase and leucine aminopeptidase activities, while SOM decomposition decreased. Several discrete peaks of glucose-derived CO2 (contributing >80% to total CO2) were observed between days 13–30 under high glucose input (300–500% of MBC), concurrently with CH4 peaks. Such CO2 dynamics was distinct from the common exponential decay pattern, implicating the recycling and mineralization of 13C-enriched microbial necromass driven by glucose addition. Therefore, N recycling from necromass was hypothesized as a major mechanism to alleviate microbial N deficiency without SOM priming under excess labile C input. Compound-specific 13C-PLFA confirmed the redistribution of glucose-derived C among microbial groups, i.e., necromass recycling. Following glucose input, more than 4/5 of total 13C-PLFA was in the gram-negative and some non-specific bacteria, suggesting these microorganisms as r-strategists capable of rapidly utilizing the most labile C. However, their 13C-PLFA content decreased by 70% after 60 days, probably as a result of death of these r-strategists. On the contrary, the 13C-PLFA in gram-positive bacteria, actinomycetes and fungi (K-strategists) was initially minimal but increased by 0.5–5 folds between days 2 and 60. Consequently, the necromass of dead r-strategists provided a high-quality C–N source to the K-strategists. We conclude that under severe C excess, N recycling from necromass is a much more efficient microbial strategy to cover the acute N demand than N acquisition from the recalcitrant SOM. © 2020 Elsevier Ltd
Scopus
Держатели документа:
Key Laboratory of Agro-ecological Processes in Subtropical Region & Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Gongdong, 510640, China
Jiangsu Provincial Key Laboratory for Bioresources of Coastal Saline Soils, Jiangsu Coastal Biological Agriculture Synthetic Innovation Center, Yancheng Teachers' University, Yancheng, 224002, China
Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China
School of Environment, Natural Resources & Geography, Bangor University, Gwynedd, LL57 2UW, United Kingdom
Department of Agricultural Soil Science, Department of Soil Science of Temperate Ecosystems, University of G?ttingen, G?ttingen, Germany
Institute of Environmental Sciences, Kazan Federal University, Kazan, 420049, Russian Federation
Agro-Technological Institute, RUDN University, Moscow, 117198, Russian Federation
Departamento de Ciencias Quimicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile
VN Sukachev Institute of Forest, SB-RAS, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Cui, J.; Zhu, Z.; Xu, X.; Liu, S.; Jones, D. L.; Kuzyakov, Y.; Shibistova, O.; Wu, J.; Ge, T.
Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
/ S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180
. - ISSN 0045-6535
Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation
Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.
Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation
Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.
Features of the Formation of Microbiomes in Two Types of Soils under Pine Provenance Trials of Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc.
/ I. D. Grodnitskaya, G. V. Kuznetsova, O. E. Pashkeeva, G. I. Antonov // Biol. Bull. - 2021. - Vol. 48, Is. 4. - P425-439, DOI 10.1134/S1062359021030067
. - ISSN 1062-3590
Аннотация: Abstract: The effect of pine provenance trials of Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc. grown in Krasnoyarsk and Khabarovsk krais on the chemical and biological parameters of gray forest and brown forest soils in the rhizosphere and between rows of plantations is compared. It is shown that the interspecies heterogeneity of pines affects the chemical composition of the soil, the enzymatic and microbiological activity, and the formation of prokaryotic microbiomes. It is noted that the biodiversity of prokaryotes is more strongly determined by the features of soil types than by the inter- and intraspecies heterogeneity of pines and by the effect of their roots. The phyla Proteobacteria, Acidobacteria, and Actinobacteria dominate in both soil types and together account for 75–85% of the total number of type-specific 16S rRNA gene sequences in each soil. © 2021, Pleiades Publishing, Inc.
Scopus
Держатели документа:
Sukachev Institute of Forests, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Grodnitskaya, I. D.; Kuznetsova, G. V.; Pashkeeva, O. E.; Antonov, G. I.
Аннотация: Abstract: The effect of pine provenance trials of Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc. grown in Krasnoyarsk and Khabarovsk krais on the chemical and biological parameters of gray forest and brown forest soils in the rhizosphere and between rows of plantations is compared. It is shown that the interspecies heterogeneity of pines affects the chemical composition of the soil, the enzymatic and microbiological activity, and the formation of prokaryotic microbiomes. It is noted that the biodiversity of prokaryotes is more strongly determined by the features of soil types than by the inter- and intraspecies heterogeneity of pines and by the effect of their roots. The phyla Proteobacteria, Acidobacteria, and Actinobacteria dominate in both soil types and together account for 75–85% of the total number of type-specific 16S rRNA gene sequences in each soil. © 2021, Pleiades Publishing, Inc.
Scopus
Держатели документа:
Sukachev Institute of Forests, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Grodnitskaya, I. D.; Kuznetsova, G. V.; Pashkeeva, O. E.; Antonov, G. I.
Features of the Formation of Microbiomes in Two Types of Soils under Pine Provenance Trials of Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc
/ I. D. Grodnitskaya, G. V. Kuznetsova, O. E. Pashkeeva, G. I. Antonov // Biol. Bull. - 2021. - Vol. 48, Is. 4. - P425-439, DOI 10.1134/S1062359021030067. - Cited References:35. - This work was supported by the Russian Foundation for Basic Research, project no. 13-04-01671.
. - ISSN 1062-3590. - ISSN 1608-3059
РУБ Biology
Аннотация: The effect of pine provenance trials of Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc. grown in Krasnoyarsk and Khabarovsk krais on the chemical and biological parameters of gray forest and brown forest soils in the rhizosphere and between rows of plantations is compared. It is shown that the interspecies heterogeneity of pines affects the chemical composition of the soil, the enzymatic and microbiological activity, and the formation of prokaryotic microbiomes. It is noted that the biodiversity of prokaryotes is more strongly determined by the features of soil types than by the inter- and intraspecies heterogeneity of pines and by the effect of their roots. The phyla Proteobacteria, Acidobacteria, and Actinobacteria dominate in both soil types and together account for 75-85% of the total number of type-specific 16S rRNA gene sequences in each soil.
WOS
Держатели документа:
Russian Acad Sci, Sukachev Inst Forests, Siberian Branch, Krasnoyarsk 660036, Russia.
Доп.точки доступа:
Grodnitskaya, I. D.; Kuznetsova, G., V; Pashkeeva, O. E.; Antonov, G., I; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [13-04-01671]
Аннотация: The effect of pine provenance trials of Pinus sibirica Du Tour and Pinus koraiensis Siebold et Zucc. grown in Krasnoyarsk and Khabarovsk krais on the chemical and biological parameters of gray forest and brown forest soils in the rhizosphere and between rows of plantations is compared. It is shown that the interspecies heterogeneity of pines affects the chemical composition of the soil, the enzymatic and microbiological activity, and the formation of prokaryotic microbiomes. It is noted that the biodiversity of prokaryotes is more strongly determined by the features of soil types than by the inter- and intraspecies heterogeneity of pines and by the effect of their roots. The phyla Proteobacteria, Acidobacteria, and Actinobacteria dominate in both soil types and together account for 75-85% of the total number of type-specific 16S rRNA gene sequences in each soil.
WOS
Держатели документа:
Russian Acad Sci, Sukachev Inst Forests, Siberian Branch, Krasnoyarsk 660036, Russia.
Доп.точки доступа:
Grodnitskaya, I. D.; Kuznetsova, G., V; Pashkeeva, O. E.; Antonov, G., I; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [13-04-01671]
Дыхательная активность и биоразнообразие микробиомов подзолистых почв постпирогенных еловых лесов Красноярского края и Республики Коми
[Текст] / И. Д. Гродницкая, О. Э. Пашкеева, В. В. Старцев, А. А. Дымов // Почвоведение. - 2023. - № 6. - С. 758-773, DOI 10.31857/S0032180X22601347
. - ISSN 0032-180X
Кл.слова (ненормированные):
ПОДЗОЛИСТЫЕ ПОЧВЫ (RETISOLS) -- СТАРОВОЗРАСТНЫЕ ЕЛЬНИКИ -- МИКРОБИОМЫ -- ПИРОГЕННЫЕ И НЕПИРОГЕННЫЕ ГОРИЗОНТЫ -- БИОРАЗНООБРАЗИЕ ПРОКАРИОТ И ГРИБОВ
Аннотация: Представлены данные по микробиологическим свойствам подзолистых почв (Retisols) старовозрастных еловых лесов на территории средней тайги Красноярского края и Республики Коми. Несмотря на различное географическое положение, почвы этих регионов характеризуются близкими морфологическими и физико-химическими свойствами. В почвах ельников Республики Коми и Красноярского края не обнаружено достоверной разницы в накоплении микробной биомассы и скорости микробного дыхания. Однако содержание в почвах углерода и азота, а также микробной биомассы оказывало значимое влияние на качественный состав микробиомов пирогенных и непирогенных горизонтов почв. Отмечено существенное влияние пирогенного фактора на α-разнообразие бактерий и грибов. Показано, что при освоении органического вещества с наличием пирогенного углерода активно участвуют представители доминантных фил бактерий (Proteobacteria, Actinobacteria и Planctomycetes) и грибов (Ascomycota, Basidiomycota и Mucoromycota). В состав микробиомов верхних пирогенных подгоризонтов входят группы карботрофных бактерий (Thermomonosporaceae, Isosphaeraceae, Bacillaceae, Xanthobacteraceae) и грибов из классов Dothideomycetes (р. Cenococcum), E-urotiomycetes (р. Penicillium), Sordariomycetes (р. Trichoderma), Leotiomycetes (р. Oidiodendron), Umbelopsidomycetes (р. Umbelopsis), которые способны к преобразованию продуктов пиролиза в доступные и нетоксичные субстраты для других организмов
Data on the microbiological properties of podzolic soils (Retisols) of old-growth spruce forests in the middle taiga of the Krasnoyarsk Krai and the Komi Republic are presented. It is shown that, despite the geographical distance, the soils of the regions are characterized by similar morphological and physicochemical properties. It was noted that in the soils of the spruce forests of the European North (R. Komi) and middle Siberia (Krasnoyarsk Krai), no significant difference in the accumulation of microbial biomass and the rate of microbial respiration was found. However, the content of carbon and nitrogen in soils, as well as microbial biomass, had significant differences in the qualitative composition of microbiomes in pyrogenic and non-pyrogenic soil horizons. A significant effect of the pyrogenic factor on the α-diversity of bacteria and fungi was noted. It was shown that representatives of the dominant phyla of bacteria (Proteobacteria, Actinobacteria and Planctomycetes) and fungi (Ascomycota, Basidiomycota and Mucoromycota) actively participate in the assimilation of organic matter with the presence of pyrogenic carbon. The microbiomes of the upper pyrogenic subhorizons include groups of carbotrophic bacteria (Thermomonosporaceae, Isosphaeraceae, Bacillaceae, Xanthobacteraceae) and fungi from the classes Dothideomycetes (Cenococcum), Eurotiomycetes (Penicillium), Sordariomycetes (Trichoderma), Leotiomycetes (Oidiodendron), Umbelopsidomycetes (Umbelopsis), which are capable of converting pyrolysis products into accessible and non-toxic substrates for other organisms
РИНЦ
Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28
Доп.точки доступа:
Пашкеева, Оксана Эриковна; Старцев, В.В.; Дымов, А.А.; Grodnitskaya, Irina Dmitriyevna
| ГРНТИ |
Кл.слова (ненормированные):
ПОДЗОЛИСТЫЕ ПОЧВЫ (RETISOLS) -- СТАРОВОЗРАСТНЫЕ ЕЛЬНИКИ -- МИКРОБИОМЫ -- ПИРОГЕННЫЕ И НЕПИРОГЕННЫЕ ГОРИЗОНТЫ -- БИОРАЗНООБРАЗИЕ ПРОКАРИОТ И ГРИБОВ
Аннотация: Представлены данные по микробиологическим свойствам подзолистых почв (Retisols) старовозрастных еловых лесов на территории средней тайги Красноярского края и Республики Коми. Несмотря на различное географическое положение, почвы этих регионов характеризуются близкими морфологическими и физико-химическими свойствами. В почвах ельников Республики Коми и Красноярского края не обнаружено достоверной разницы в накоплении микробной биомассы и скорости микробного дыхания. Однако содержание в почвах углерода и азота, а также микробной биомассы оказывало значимое влияние на качественный состав микробиомов пирогенных и непирогенных горизонтов почв. Отмечено существенное влияние пирогенного фактора на α-разнообразие бактерий и грибов. Показано, что при освоении органического вещества с наличием пирогенного углерода активно участвуют представители доминантных фил бактерий (Proteobacteria, Actinobacteria и Planctomycetes) и грибов (Ascomycota, Basidiomycota и Mucoromycota). В состав микробиомов верхних пирогенных подгоризонтов входят группы карботрофных бактерий (Thermomonosporaceae, Isosphaeraceae, Bacillaceae, Xanthobacteraceae) и грибов из классов Dothideomycetes (р. Cenococcum), E-urotiomycetes (р. Penicillium), Sordariomycetes (р. Trichoderma), Leotiomycetes (р. Oidiodendron), Umbelopsidomycetes (р. Umbelopsis), которые способны к преобразованию продуктов пиролиза в доступные и нетоксичные субстраты для других организмов
Data on the microbiological properties of podzolic soils (Retisols) of old-growth spruce forests in the middle taiga of the Krasnoyarsk Krai and the Komi Republic are presented. It is shown that, despite the geographical distance, the soils of the regions are characterized by similar morphological and physicochemical properties. It was noted that in the soils of the spruce forests of the European North (R. Komi) and middle Siberia (Krasnoyarsk Krai), no significant difference in the accumulation of microbial biomass and the rate of microbial respiration was found. However, the content of carbon and nitrogen in soils, as well as microbial biomass, had significant differences in the qualitative composition of microbiomes in pyrogenic and non-pyrogenic soil horizons. A significant effect of the pyrogenic factor on the α-diversity of bacteria and fungi was noted. It was shown that representatives of the dominant phyla of bacteria (Proteobacteria, Actinobacteria and Planctomycetes) and fungi (Ascomycota, Basidiomycota and Mucoromycota) actively participate in the assimilation of organic matter with the presence of pyrogenic carbon. The microbiomes of the upper pyrogenic subhorizons include groups of carbotrophic bacteria (Thermomonosporaceae, Isosphaeraceae, Bacillaceae, Xanthobacteraceae) and fungi from the classes Dothideomycetes (Cenococcum), Eurotiomycetes (Penicillium), Sordariomycetes (Trichoderma), Leotiomycetes (Oidiodendron), Umbelopsidomycetes (Umbelopsis), which are capable of converting pyrolysis products into accessible and non-toxic substrates for other organisms
РИНЦ
Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28
Доп.точки доступа:
Пашкеева, Оксана Эриковна; Старцев, В.В.; Дымов, А.А.; Grodnitskaya, Irina Dmitriyevna