Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / T. S. Sedel'nikova, A. V. Pimenov // Biol. Bull. - 2005. - Vol. 32, Is. 1. - P16-21, DOI 10.1007/s10525-005-0004-7. - Cited References: 23 . - 6. - ISSN 1062-3590РУБ Biology

Аннотация: The karyological data on populations of Siberian fir (Abies sibirica Ledeb.) growing in lowland swamp and dry valleys are given. The diploid set of both populations included 24 chromosomes (2n = 24). Polykaryogram analysis revealed seven pairs of metacentric and five pairs of submetacentric chromosomes. The revealed differences between the populations included the absolute length of chromosomes, number of nucleolar organizer regions, and number of nucleoli. Changed chromosome numbers (mixoploidy and aneuploidy) as well as chromosomal aberrations were recorded. For the first time, mitosis was studied in this species and anaphase/telophase aberrations were revealed. The population of Siberian fir growing under extreme conditions of lowland swamp featured the widest range of mutations.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Siberian Div, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Sedel'nikova, T.S.; Pimenov, A.V.

/ O. V. Goryachkina [et al.] // Cytologia. - 2018. - Vol. 83, Is. 3. - P322-328, DOI 10.1508/cytologia.83.323. - Cited References:34. - Authors express sincerely thanks to Dr. Oreshkova N.V., V. N. Sukachev Institute of Forest SB RAS, for supplying of L. sibirica genomic DNA, and Dr. Shcherban A.B., The Federal Research Center Institute of Cytology and Genetics SB RAS for preparation of 5S rDNA probe. The study was funded by Russian Foundation for Basic Research and Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support (project No 16-44-243068), Russian Foundation for Basic Research and Belarus (project 18-54-00010), Investment Foundation FASIE (project 0040522). . - ISSN 0011-4545РУБ Cell Biology + Genetics & Heredity

Аннотация: We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91-96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Krasnoyarsk Sci Ctr, Siberian Branch,Fed Res Ctr, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Vavilov Inst Gen Genet, Gubkin Str 3, Moscow 119333, Russia.

Доп.точки доступа:
Goryachkina, Olga V.; Park, Maria E.; Tretyakova, Iraida N.; Badaeva, Ekaterina D.; Muratova, Elena N.; Russian Foundation for Basic Research; Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support [16-44-243068]; Investment Foundation FASIE [0040522]; [18-54-00010]

[Текст] : научное издание / Е. Н. Муратова, Т. С. Седельникова, О. В. Горячкина, А. В. Пименов // сибирский экологический журнал. - 2023. - Т. 30, № 5. - С. 591-602, DOI 10.15372/SEJ20230502 . - ISSN 0869-8619

ГРНТИ

68.47.03

Аннотация: Обобщены результаты кариологических и цитогенетических исследований в популяциях видов хвойных семейств Pinaceae и Cupressaceae из экстремальных условий произрастания. У хвойных, произрастающих у южной и северной границ ареалов на экологическом пределе распространения, в антропогенно нарушенных экосистемах, в условиях интродукции, выявлена хромосомная изменчивость, обнаружены геномные и хромосомные мутации различного типа. Отмечены высокая частота изменений числа хромосом (миксоплоидия, анеуплоидия и полиплоидия) и их морфологии. Найдены различные типы хромосомных аномалий (кольцевые и полицентрические хромосомы, фрагменты, множественные хромосомные аномалии), агглютинация хромосом, появление В-хромосом, нарушения митоза и мейоза. С использованием классических методов и флуоресцентной гибридизации in situ (FISH) проведены исследования полиморфизма нуклеолярных районов хромосом, выявлены особенности локусов генов 5S и 45S рибосомной РНК.
Results of karyological and cytogenetical studies in populations of conifer species of Pinaceae and Cupressaceae families from extreme conditions are summarized. In conifers growing in southern and northern borders of areas, on ecological limits of distribution, in anthropogenically disturbed ecosystems, at the introduction, chromosome variability is revealed. A high frequency of changes in the number of chromosomes (mixoploidy, aneuploidy and polyploidy) and their morphology were noted. Different types of chromosome anomalies (ring and polycentric chromosomes, fragments, multiple chromosome irregularities), B-chromosomes occurrence, irregularities of mitosis and meiosis are found. Using classical methods and fluorescent in situ hybridization (FISH), studies of the polymorphism of the nucleolar regions of chromosomes were carried out, and features of the loci of the 5S and 45S ribosomal RNA genes were revealed.

РИНЦ

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Седельникова, Тамара Станиславовна; Sedel'nikova, Tamara Stanislavovna; Горячкина, Ольга Викторовна; Пименов, Александр Владимирович; Pimenov, Alexandr Vladimirovich; Muratova, Elena Nikolayevna