Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

: материалы временных коллективов / И. Н. Павлов, О. А. Барабанова, В. В. Кузьмичев, А. А. Мочалов // Лесной и химический комплексы - проблемы и решения. Всероссийская научно-практическая конференция 12-14 октября 2005 г., посвященная 75-летию СибГТУ. - Красноярск : СибГТУ, 2005. - Т. 2. - С. 235-244. - Библиогр. в конце ст.
Аннотация: На примере 53-летних лесных культур сосны обыкновенной с помощью геоинформационных технологий проведен анализ внутриценотического взаимодействия. Из всех изученных параметров наибольшее влияние на рост оказывают количество деревьев-"соседей", их размеры и расстояние до модельных экземпляров. В результате дисперсионного анализа установлено, что влияние деревьев-"соседей" объясняет почти половину дисперсии толщины ствола (42,6 %, р0,001). В результате воздействия патогенных дереворазрушающих грибов (виды комплекса Armillaria mellea sensu lato; Heterobasidion annosum (Fr.) Bref.) произошло некоторое снижение установленной закономерности влияния деревьев-"соседей" на рост модельных особей по диаметру. Так если в 1981 г. связь между количеством "соcедей" и диаметром модельного дерева составляет - 0,59, то к 2003 году коэффициент корреляции снижается до -0,50 (р0,001). По результатам дисперсионного анализа установлено снижение доли влияния количества расположенных рядом деревьев (до 24,0 %, р0,001).

Держатели документа:
Институт леса им. В.Н. Сукачева Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок 50/28

Доп.точки доступа:
Барабанова, О.А.; Barabanova O.A.; Кузьмичев, Валерий Васильевич; Kuz'michev, Valery Vasil'yevich; Мочалов, А.А.; Mochalov A.A.; Pavlov Igor' Nikolayevich
Имеются экземпляры в отделах:
РСФ (20.11.2006г. (1 экз.) - Б.ц.) - свободны 1

[Текст] : материалы временных коллективов / И. Н. Павлов, С. С. Кулаков [и др.] // Хвойные бореальной зоны. - 2012. - Т. XXX, № 3-4. - С. 234-246. - Библиогр. в конце ст.
Аннотация: Установлена определяющая роль Armillaria borealis Marxm. & Korhonen в куртинном усыхании хвойных насаждений. В основном очаги усыхания образовались без какого-либо заметного предварительного ослабления древостоя в период 2002-2005 гг. и также быстро, практически одновременно (2007-2011 гг.) на всей исследуемой территории произошло их затухание в сочетании с улучшением состояния насаждения, что следует рассматривать как успешную перестройку биоценоза, его переход на новый уровень стабильности, соответствующий потенциалу биотопа. В настоящее время отпад в пределах естественного и происходит преимущественно за счет деревьев низших классов Крафта. Очаги усыхания в хвойных древостоях сформировались на маломощных, более кислых почвах с повышенной влажностью и с большим содержанием глинистой фракции. За пределы данной эдафической аномалии усыхание деревьев I -II классов Крафта не распространяется. Выявленную приуроченность очагов куртинного усыхания следует использовать при разработке хозяйственных решений для снижения патогенного воздействия опенка на хвойные древостои.

Держатели документа:
Институт леса им. В.Н. Сукачева Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок 50/28

Доп.точки доступа:
Кулаков, С.С.; Евдокимова, Л.С.; Кудрявцев, О.А.; Перцовая, А.А.; Pavlov Igor' Nikolayevich

[Текст] / И. Н. Павлов, А. Г. Цыкалов // Лесные биогеоценозы бореальной зоны: география, структура, функции, динамика. Материалы Всероссийской научной конференции с международным участием, посвященной 70-летию создания Института леса им. В.Н. Сукачева СО РАН 16-19 сентября 2014 г., Красноярск. - Новосибирск : Изд-во СО РАН, 2014. - С. 35-38. - Библиогр. в конце ст.
Аннотация: Установлена определяющая роль корневых патогенов (Armillaria mellea s.l., Heterobasidion annosum s.l., Phellinus sulphurascens Pilat., Porodaedalea niemelaei M. Fischer) в усыхании хвойных лесов Сибири и Дальнего Востока. Наибольшую опасность представляет A. borealis, особенно для одновозрастных приспевающих — спелых насаждений Pinus sibirica и Abies sibirica.

Полный текст

Держатели документа:
Институт леса им. В.Н. Сукачева СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Павлов, Игорь Николаевич; Цыкалов, Анатолий Григорьевич; Tsykalov Anatoly Grigor'yevich; Pavlov Igor' Nikolayevich

[Текст] / А. И. Татаринцев, Л. Н. Скрипальщикова // Сибирский лесной журнал. - 2015. - № 2. - С. 8-19DOI 10.15372/SJFS20150201. - Библиогр. в конце ст. . -
Аннотация: По данным проведенного обследования санитарное и жизненное состояние березовых (Betula pendula Roth.) насаждений в лесах Красноярской группы районов (южная часть Средней Сибири) в целом оценивается как удовлетворительное, но около половины из тех, что приближены к урботерриториям, являются ослабленными. Состояние насаждений достоверно ухудшается с повышением рекреационной нагрузки, хотя влияние техногенного загрязнения незначительное. Среди выявленных на березе представителей патогенной биоты наиболее значимы возбудители некрозно-раковых и гнилевых болезней. В березняках обнаружено повсеместное распространение бактериальной водянки (возбудитель Erwinia multivora Scz.-Parf.), распространенность болезни – от единичных больных деревьев до очагового поражения (10–38 %). В большей степени поражены березняки в таежных лесах в сравнении с лесостепью и высокобонитетные насаждения, произрастающие на влажных почвах. Распространенность бактериоза нарастает с повышением возраста и полноты древостоев и не зависит от уровня рекреационной нагрузки. Пораженные водянкой деревья являются фактическим или потенциальным отпадом. В таежных березняках поражение и загнивание корней вызывает опенок осенний (Armillaria mellea sensu lato), что приводит к единичному, реже групповому усыханию деревьев. Гриб обычно элиминирует предварительно ослабленные экземпляры. В деструкции древесной биомассы участвует комплекс афиллофороидных гименомицетов, полупаразитные представители которых вызывают развитие стволовой гнили, понижающей товарность древостоев и приводящей к накоплению гнилевого ветролома. Распространенность гнили достоверно выше в порослевых березняках – до 20 % и более. Связи пораженности гнилью с таксационными показателями древостоев не установлено.

Полный текст

Держатели документа:
Институт леса имени В.Н. Сукачева СО РАН : 660036, г. Красноярск, Академгородок №50, стр.28

Доп.точки доступа:
Скрипальщикова, Лариса Николаевна; Skripalshchikova, Larisa Nikolayevna; Tatarintsev Andrey Ivanovich

[Текст] / И. Н. Павлов // Сибирский экологический журнал. - 2015. - Т. 22, № 4. - С. 537-554DOI 10.15372/SEJ20150405. - Библиогр. в конце ст. . -
Аннотация: На основе результатов многолетних исследований выявлены причины и механизмы массового усыхания хвойных лесов Сибири и Дальнего Востока ( Pinus sibirica Du Tour, Picea obovata Ledeb., Abies sibirica Ledeb., Pinus sylvestris L., Larix gmelinii (Rupr.) Kuzen., Abies nephrolepis (Trautv. ex Maxim.) Maxim., Pinus koraiensis Siebold & Zucc.) в последние два десятилетия. Установлено, что гибель вызвана патогенным действием возбудителей корневых гнилей ( Armillaria mellea s.l., Heterobasidion annosum s.l., Phellinus sulphurascens Pilat., Porodaedalea niemelaei M. Fischer, Phaeolus schweinitzii (Fr.) Pat.) на фоне снижения биологической устойчивости хвойных деревьев. Наиболее опасным, поражающим большое количество видов древесных растений в различных лесорастительных условиях, следует считать Armillaria borealis Marxm. & Korh. Триггерным механизмом усыхания является сочетание неблагоприятных для деревьев климатических аномалий в сочетании с определенными эдафическими условиями и (или) комплекс благоприятных факторов для патогенных организмов.

Полный текст,
РИНЦ

Держатели документа:
Институт леса имени В.Н. Сукачева СО РАН : 660036, г. Красноярск, Академгородок №50, стр.28

Доп.точки доступа:
Pavlov Igor' Nikolayevich

/ I. N. Pavlov // Contemp. Probl. Ecol. - 2015. - Vol. 8, Is. 4. - P440-456, DOI 10.1134/S1995425515040125 . - ISSN 1995-4255
Аннотация: This study is based on 20 years of research into the massive dieback of coniferous forests (Pinus sibirica Du Tour, Picea obovata Ledeb., Abies sibirica Ledeb., Pinus sylvestris L., Larix gmelinii (Rupr.) Kuzen., Abies nephrolepis (Trautv. ex Maxim.) Maxim., Pinus koraiensis Siebold & Zucc.) in Siberia and the Far East. It was found that the dieback had been provoked by the causative agents of root rot disease (Armillaria mellea s.l, Heterobasidion annosum s.l, Phellinus sulphurascens Pilat., Porodaedalea niemelaei M. Fischer, Phaeolus schweinitzii (Fr.) Pat.). The disease spread due to the decreased biological sustainability of coniferous trees. A. borealis Marxm. & Korh. should be considered the most dangerous species affecting a large variety of woody plants in different forest-site conditions. The trigger mechanism of the dieback was a combination of adverse climatic anomalies and certain edaphic conditions and/or a set of factors favorable for pathogenic organisms. © 2015, Pleiades Publishing, Ltd.

Scopus,
WOS,
Полный текст

Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Pavlov, I. N.

/ Y. A. Litovka [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2017. - Is. 1. - С. 145-150, DOI 10.14258/jcprm.2017011577 . - ISSN 1029-5151
Аннотация: Presents the results of a research of wood-destroying properties Porodaedalea niemelaei M. Fischer and Trichoderma atroviride Bissett. The strains were isolated in pure culture from the fruiting body and larch wood (Larix gmelinii (Rupr.) Rupr.) in permafrost. Studied phenol oxidase activity and biodegradation of waste wood at their solid-state fermentation. Under laboratory conditions, fungi colonize deciduous and coniferous substrates in monoculture and co-culture. Bioconversion depends on the enzymatic activity of strains and chemical characteristics of the growth substrate. Maximum of wooddestroying activity observed in strain PnB-13 Porodaedalea niemelaei on sawdust of aspen. The loss of weight of the substrate was 39%; polysaccharide content decreased to 1,6 times in comparison with the initial substrate; lignin - in 1.2 times. The concentration of water-soluble substances increased 1,5 times. P. niemelaei and T. atroviride produce extracellular oxidase of lignolytic complex in the solid-phase cultivation of plant residues. Enzyme activity was 0,15-0,22 u/g.s on sawdust of larch and 0,14-0,18 u/g.s on sawdust of aspen, which is comparable to the phenol oxidase activity of other xylotrophic basidiomycetes (Trametes versicolor, Armillaria mellea s.l., Heterobasidion parviporum).

Scopus,
Смотреть статью

Держатели документа:
Siberian State Aerospace University. Academician M.F. Reshetnev, pr. Mira, 82, Krasnoyarsk, Russian Federation
Institute of Forest. V.N. Sukachev, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/28, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Litvinova, E. A.; Chuprova, N. A.

/ A. I. Kolesnikova [et al.] // BMC Genomics. - 2019. - Vol. 20. - Ст. 351, DOI 10.1186/s12864-019-5732-z. - Cited References:80. - This study was funded by the Research Grant No. 14.Y26.31.0004 from the Government of the Russian Federation. The funding body did not contribute in the design of the study, collection, analysis, interpretation of data, or writing the manuscript. . - ISSN 1471-2164РУБ Biotechnology & Applied Microbiology + Genetics & Heredity

Аннотация: BackgroundSpecies in the genus Armillaria (fungi, basidiomycota) are well-known as saprophytes and pathogens on plants. Many of them cause white-rot root disease in diverse woody plants worldwide. Mitochondrial genomes (mitogenomes) are widely used in evolutionary and population studies, but despite the importance and wide distribution of Armillaria, the complete mitogenomes have not previously been reported for this genus. Meanwhile, the well-supported phylogeny of Armillaria species provides an excellent framework in which to study variation in mitogenomes and how they have evolved over time.ResultsHere we completely sequenced, assembled, and annotated the circular mitogenomes of four species: A. borealis, A. gallica, A. sinapina, and A. solidipes (116,443, 98,896, 103,563, and 122,167bp, respectively). The variation in mitogenome size can be explained by variable numbers of mobile genetic elements, introns, and plasmid-related sequences. Most Armillaria introns contained open reading frames (ORFs) that are related to homing endonucleases of the LAGLIDADG and GIY-YIG families. Insertions of mobile elements were also evident as fragments of plasmid-related sequences in Armillaria mitogenomes. We also found several truncated gene duplications in all four mitogenomes.ConclusionsOur study showed that fungal mitogenomes have a high degree of variation in size, gene content, and genomic organization even among closely related species of Armillara. We suggest that mobile genetic elements invading introns and intergenic sequences in the Armillaria mitogenomes have played a significant role in shaping their genome structure. The mitogenome changes we describe here are consistent with widely accepted phylogenetic relationships among the four species.

WOS,
Смотреть статью,
Scopus

Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Genome Res & Educ Ctr, Lab Forest Genom, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Lab Genom Res & Biotechnol, Siberian Branch,Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Anim Systemat & Ecol, Siberian Branch, Novosibirsk 630091, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, Lab Forest Genet & Select, Siberian Branch, Krasnoyarsk 660036, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, Lab Reforestat Mycol & Plant Pathol, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Inst Space & Informat Technol, Dept High Performance Comp, Krasnoyarsk 660074, Russia.
Univ Toronto, Dept Biol, Mississauga, ON 15L 1C6, Canada.
Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, D-37077 Gottingen, Germany.
Russian Acad Sci, NI Vavilov Inst Gen Genet, Lab Populat Genet, Moscow 119333, Russia.
Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA.

Доп.точки доступа:
Kolesnikova, Anna, I; Putintseva, Yuliya A.; Simonov, Evgeniy P.; Biriukov, Vladislav V.; Oreshkova, Natalya, V; Pavlov, Igor N.; Sharov, Vadim V.; Kuzmin, Dmitry A.; Anderson, James B.; Krutovsky, Konstantin, V; Krutovsky, Konstantin; Government of the Russian Federation [14, Y26.31.0004]

/ V. P. Zhelifonova [et al.] // Appl. Biochem. Microbiol. - 2019. - Vol. 55, Is. 3. - P277-283, DOI 10.1134/S0003683819030153. - Cited References:18 . - ISSN 0003-6838. - ISSN 1608-3024РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: Secondary metabolites of the basidiomycetes of Armillaria borealis Marxm. and Korhonen, A.cepistipes Velen., A. gallica Marxm., A. ostoyae (Romagn.) Herink, and A. sinapina Berube and Dessur isolated in Southern Siberia (Krasnoyarsk region and Tyva Republic) and in the Far East (Sikhote-Alin) were studied. Metabolites belonging to the class of protoilludene sesquiterpene aryl esters of the melleolides group have been identified in the species A. borealis, A. cepistipes, and A. sinapina. The strains differ in the spectrum of synthesized melleolides. A. borealis strain 74g synthesized a wider range of melleolides than other strains of the species: melleolides B, C, D and H, melledonals B and C, 5'-O-methylmelledonal, 13-hydroxy-5'-O-methylmelledonal, and armillarinin. It was shown that the composition of the medium influenced the amount of synthesized metabolites. All of the studied strains synthesizing melleolides exhibited a toxicogenic and phytopathogenic effect on seeds and seedlings of Picea abies (L.) H. Karst. and Abies sibirica Ledeb. A significant decrease in seed germination energy, laboratory and ground seed germination, the development of the root system of conifer seedlings was revealed. The maximum inhibitory effect was shown by the culture fluid of A. borealis 74g with the greatest amounts and variety of melleolides in the metabolome profile.

WOS,
Смотреть статью,
Scopus

Держатели документа:
Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Zhelifonova, V. P.; Antipova, T. V.; Litvinova, E. A.; Baskunov, B. P.; Litovka, Yu. A.; Pavlov, I. N.; Kozlovsky, A. G.

/ I. N. Pavlov, Y. A. Litovka, D. V. Golubev [et al.] // Contemp. Probl. Ecol. - 2020. - Vol. 13, Is. 1. - P71-84, DOI 10.1134/S1995425520010060. - Cited References:59 . - ISSN 1995-4255. - ISSN 1995-4263РУБ Ecology

Аннотация: Conifer decline (Abies sibirica Ledeb. stands) as a result of complex biotic effects on the territory of Krasnoyarsk krai by 2018 reached an area of 541 400 ha; the potential area of further death of A. sibirica is up to 10 million ha. The main reason is the phytopathogenic fungi Armillaria mellea s.l. and Heterobasidion annosum s.l. combined with bark beetle Polygraphus proximus. Trees affected by root pathogens are centers of forest dieback, from which pathological outbreaks spread, including the effects of P. proximus and its phytopathogenic mycoassociates. Neonectria fuckeliana (C. Booth) Castl & Rossman is among the concomitant pathogens in the scale of the lesion, which causes a resin flow that is similar to the effects of P. proximus attack. The phytopathogenicity of pure cultures of the dominant root pathogens of Armillaria borealis Marxm. & Korhonen and Heterobasidion annosum (Fr.) Bref., as well as the stem pathogen of N. fuckeliana, has been confirmed by a series of experiments in vitro. Promising natural agents for the regulation of P. proximus abundance are highly virulent aboriginal strains of Beauveria bassiana (Bals.-Criv.) Vuill., resulting in maximum insect death (up to 100%) for 7-11 days and retaining activity in the temperature range of 16-24 +/- 1 degrees C.

WOS

Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660037, Russia.
Federal Forestry Agcy, Russian Ctr Forest Hlth Branch, Ctr Forest Hlth Krasnoyarsk Krai, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Pavlov, I. N.; Litovka, Y. A.; Golubev, D. V.; Astapenko, S. A.; Chromogin, P. V.; Usoltseva, Y. V.; Makolova, P. V.; Petrenko, S. M.

/ V. S. Akulova, V. V. Sharov, A. I. Aksyonova [et al.] // BMC Genomics. - 2020. - Vol. 21. - Ст. 534, DOI 10.1186/s12864-020-06964-6. - Cited References:48. - This work including the study and collection, analysis and interpretation of data, and writing the manuscript was supported by research grant. 14.Y26.31.0004 from the Government of the Russian Federation with partial funding from the Federal Research Center "Krasnoyarsk Scientific Center", Siberian Branch, Russian Academy of Sciences (grants No 0287-2019-0002, No 0356-2016-0704, and No 0356-2019-0024). The funding agencies played no role in the design of the study and collection material, analysis and interpretation of data, and in writing the manuscript. Publication cost have been funded by the Open Access Publication Funds of the University of Gottingen. . - ISSN 1471-2164РУБ Biotechnology & Applied Microbiology + Genetics & Heredity

Аннотация: Background: Massive forest decline has been observed almost everywhere as a result of negative anthropogenic and climatic effects, which can interact with pests, fungi and other phytopathogens and aggravate their effects. Climatic changes can weaken trees and make fungi, such as Armillaria more destructive. Armillaria borealis (Marxm. & Korhonen) is a fungus from the Physalacriaceae family (Basidiomycota) widely distributed in Eurasia, including Siberia and the Far East. Species from this genus cause the root white rot disease that weakens and often kills woody plants. However, little is known about ecological behavior and genetics of A. borealis. According to field research data, A. borealis is less pathogenic than A. ostoyae, and its aggressive behavior is quite rare. Mainly A. borealis behaves as a secondary pathogen killing trees already weakened by other factors. However, changing environment might cause unpredictable effects in fungus behavior. ResultsThe de novo genome assembly and annotation were performed for the A. borealis species for the first time and presented in this study. The A. borealis genome assembly contained similar to 68 Mbp and was comparable with similar to 60 and similar to 79.5 Mbp for the A. ostoyae and A. mellea genomes, respectively. The N50 for contigs equaled 50,544bp. Functional annotation analysis revealed 21,969 protein coding genes and provided data for further comparative analysis. Repetitive sequences were also identified. The main focus for further study and comparative analysis will be on the enzymes and regulatory factors associated with pathogenicity. ConclusionsPathogenic fungi such as Armillaria are currently one of the main problems in forest conservation. A comprehensive study of these species and their pathogenicity is of great importance and needs good genomic resources. The assembled genome of A. borealis presented in this study is of sufficiently good quality for further detailed comparative study on the composition of enzymes in other Armillaria species. There is also a fundamental problem with the identification and classification of species of the Armillaria genus, where the study of repetitive sequences in the genomes of basidiomycetes and their comparative analysis will help us identify more accurately taxonomy of these species and reveal their evolutionary relationships.

WOS

Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Forest Genom, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr, Siberian Branch, Lab Genom Res & Biotechnol,Fed Res Ctr, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Inst Space & Informat Technol, Dept High Performance Comp, Krasnoyarsk 660074, Russia.
Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forest, Lab Forest Genet & Select, Krasnoyarsk 660036, Russia.
Natl Res Tech Univ, Dept Informat, Irkutsk 664074, Russia.
Russian Acad Sci, Siberian Branch, Limnol Inst, Irkutsk 664033, Russia.
Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forest, Lab Reforestat Mycol & Plant Pathol, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Dept Chem Technol Wood & Biotechnol, Krasnoyarsk 660049, Russia.
Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, D-37077 Gottingen, Germany.
George August Univ Gottingen, Ctr Integrated Breeding Res, D-37075 Gottingen, Germany.
Russian Acad Sci, NI Vavilov Inst Gen Genet, Lab Populat Genet, Moscow 119333, Russia.
Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA.

Доп.точки доступа:
Akulova, Vasilina S.; Sharov, Vadim V.; Aksyonova, Anastasiya I.; Putintseva, Yuliya A.; Oreshkova, Natalya V.; Feranchuk, Sergey I.; Kuzmin, Dmitry A.; Pavlov, Igor N.; Litovka, Yulia A.; Krutovsky, Konstantin V.; Krutovsky, Konstantin; Government of the Russian Federation [14.Y26.31.0004]; Federal Research Center "Krasnoyarsk Scientific Center", Siberian Branch, Russian Academy of Sciences [0287-2019-0002, 0356-2016-0704, 0356-2019-0024]; University of Gottingen

/ Yu. A. Litovka, I. N. Pavlov, P. V. Makolova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 371-383, DOI 10.14258/JCPRM.2020048396 . - ISSN 1029-5151
   Перевод заглавия: Биотехнологический потенциал сибирских штаммов базидиальных грибов - продуцентов ферментов лигноцеллюлазного действия
Аннотация: The results of a study of the wood-destroying properties of Siberian strains of xylotrophic basidiomycetes (Armillaria, Ganoderma, Fomitopsis, Heterobasidion and Porodaedalea) are presented. The growth parameters and enzymatic activity of the strains were determined during solid-phase and deep cultivation. Fomitopsis pinicola and Ganoderma lucidum are fast-growing fungi on cellulose-, tannin-containing nutrient media, coniferous and deciduous plant substrates (source and hydrodynamically activated). The growth coefficient on media with tannin and Na-carboxymethyl cellulose is more than 45; on plant substrates - more than 30. The strains exhibit a different growth reaction to the preliminary activation of birch sawdust (growth acceleration / growth slowdown / indifference). The maximum wood-destroying activity on wood of A. sibirica noted for the fungi A. borealis, G. tsugae, G. lucidum, F. pinicola, and P. niemelaei. The decrease in substrate mass was 8-11%; the amount of polysaccharides decreased on average 1.4 times mainly due to the fermentolysis of hard-hydrolyzable polysaccharides. High enzymatic activity of fungi observed during solid-phase and deep cultivation with an inducer. The maximum activity of phenol oxidase is characteristic of G. tsugae (1.21 units/g·s); carboxymethyl cellulase - for F.pinicola and G. lucidum strains (11.8 and 10.3 units/ml, respectively); xylanases - for H. abietinum (3.8 u/ml). The maximum accumulation of extracellular protein observed in F. pinicola (0.89 mg/ml). According to the totality of rapid test indicators, quantitative determination of enzymatic activity, growth parameters on lignocellulosic substrates and the degree of wood bioconversion, the most promising producer of lignolytic enzymes in vitro is the Gl4-16A Ganoderma lucidum strain; cellulolytic enzymes - strain Fp6-17 Fomitopsis pinicola. © 2020 Altai State University. All rights reserved.

Scopus

Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Institute of Physics L.V. Kirensky, SB, RAS, Akademgorodok, 50/43, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Litovka, Yu. A.; Pavlov, I. N.; Makolova, P. V.; Timofeev, A. A.; Litvinova, E. A.; Vasil'Eva, A. A.; Shabanov, A. V.

/ R. Linnakoski, S. Sutela, MPA Coetzee [et al.] // Sci Rep. - 2021. - Vol. 11, Is. 1. - Ст. 7336, DOI 10.1038/s41598-021-86343-7. - Cited References:88. - Tuija Hytonen, Alex Nordlund and Runlei Chang are thanked for excellent technical assistance and Pyry Veteli for providing Finnish isolates of Armillaria. The CSC - IT Center for Science, Finland, is acknowledged for providing computational resources. This work was funded by the Academy of Finland (decision number 309896). We are grateful to the 1KFG project (CSP 1974) for access to unpublished genome data. The genome sequence data were produced by the US Department of Energy Joint Genome Institute in collaboration with the user community, and we acknowledge the JGI team and the people who generated the material and RNA for the project: Francis M. Martin, Laszlo Nagy, Neha Sahu, Sara Hortal Botifoll, Johanna Wong-Bajracharya and Jonathan M. Plett. . - ISSN 2045-2322РУБ Multidisciplinary Sciences

Аннотация: Species of Armillaria are distributed globally and include some of the most important pathogens of forest and ornamental trees. Some of them form large long-living clones that are considered as one of the largest organisms on earth and are capable of long-range spore-mediated transfer as well as vegetative spread by drought-resistant hyphal cords called rhizomorphs. However, the virus community infecting these species has remained unknown. In this study we used dsRNA screening and high-throughput sequencing to search for possible virus infections in a collection of Armillaria isolates representing three different species: Armillaria mellea from South Africa, A. borealis from Finland and Russia (Siberia) and A. cepistipes from Finland. Our analysis revealed the presence of both negative-sense RNA viruses and positive-sense RNA viruses, while no dsRNA viruses were detected. The viruses included putative new members of virus families Mymonaviridae, Botourmiaviridae and Virgaviridae and members of a recently discovered virus group tentatively named "ambiviruses" with ambisense bicistronic genomic organization. We demonstrated that Armillaria isolates can be cured of viruses by thermal treatment, which enables the examination of virus effects on host growth and phenotype using isogenic virus-infected and virus-free strains.

WOS

Держатели документа:
Nat Resources Inst Finland Luke, Helsinki, Finland.
Univ Pretoria, Forestry & Agr Biotechnol Inst FABI, Dept Biochem Genet & Microbiol, Pretoria, South Africa.
VN Sukachev Inst Forest SB RAS, Lab Reforestat Mycol & Plant Pathol, Krasnoyarsk, Russia.
Reshetnev Siberian State Univ Sci & Technol, Dept Chem Technol Wood & Biotechnol, Krasnoyarsk, Russia.

Доп.точки доступа:
Linnakoski, Riikka; Sutela, Suvi; Coetzee, Martin P. A.; Duong, Tuan A.; Pavlov, Igor N.; Litovka, Yulia A.; Hantula, Jarkko; Wingfield, Brenda D.; Vainio, Eeva J.; Litovka, Yulia; Academy of FinlandAcademy of FinlandEuropean Commission [309896]; 1KFG project [CSP 1974]

/ R. Linnakoski, S. Sutela, M. P.A. Coetzee [et al.] // Ботанический журнал. - 2021. - Т. 106, № 4. - P353-362, DOI 10.31857/S0006813621040116 . - ISSN 0006-8136
Аннотация: Species of Armillaria are distributed globally and include some of the most important pathogens of forest and ornamental trees. Some of them form large long-living clones that are considered as one of the largest organisms on earth and are capable of long-range spore-mediated transfer as well as vegetative spread by drought-resistant hyphal cords called rhizomorphs. However, the virus community infecting these species has remained unknown. In this study we used dsRNA screening and high-throughput sequencing to search for possible virus infections in a collection of Armillaria isolates representing three different species: Armillaria mellea from South Africa, A. borealis from Finland and Russia (Siberia) and A. cepistipes from Finland. Our analysis revealed the presence of both negative-sense RNA viruses and positive-sense RNA viruses, while no dsRNA viruses were detected. The viruses included putative new members of virus families Mymonaviridae, Botourmiaviridae and Virgaviridae and members of a recently discovered virus group tentatively named “ambiviruses” with ambisense bicistronic genomic organization. We demonstrated that Armillaria isolates can be cured of viruses by thermal treatment, which enables the examination of virus effects on host growth and phenotype using isogenic virus-infected and virus-free strains. © 2021, The Author(s).

Scopus

Держатели документа:
Natural Resources Institute Finland (Luke), Helsinki, Finland
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa
Laboratory of Reforestation, Mycology and Plant Pathology, V.N. Sukachev Institute of Forest SB RAS, Krasnoyarsk, Russian Federation
Department of Chemical Technology of Wood and Biotechnology, Reshetnev Siberian State University of Science and Technology, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Linnakoski, R.; Sutela, S.; Coetzee, M. P.A.; Duong, T. A.; Pavlov, I. N.; Litovka, Y. A.; Hantula, J.; Wingfield, B. D.; Vainio, E. J.

/ T. V. Antipova, V. P. Zhelifonova, Y. A. Litovka [et al.] // iForest. - 2022. - Vol. 15. - P38-46, DOI 10.103832/ifor3840014. - Cited References:28 . - ISSN 1971-7458РУБ Forestry

Аннотация: Basidiomycetes Armillaria infect deciduous, coniferous and fruit trees, causing enormous economic damage. The role of secondary metabolites (tricyclic sesquiterpene aryl esters - melleolides) in the life cycle and pathogenesis of Armillaria is under active investigation. To date, not all species of Armillaria have been tested for the biosynthesis of melleolides. We investigated the secondary metabolite profiles of six root-pathogenic species of the genus Armillaria (A. borealis Marxmuller & Korhonen, A. cepistipes Velenovsky, A. gallica Marxm, A. mellea (Vahl) P. Kummer, A. sinapina Berube & Dessur, A. ostoyae (Romagn.) Herink) distributed in Siberia (South Krasnoyarsk Krai, Republic of Tyva, Republic of Khakassia, Taimyr Peninsula), Russian Far East (Sikhote-Alin) and Crimea (Krymsky National Park, Chatyr-Dag Mountain Lower Plateau). A total of 15 compounds were identified in the metabolome profile. Two compounds (melleolide D and melledonal C) are synthesized by all investigated strains irrespective of their geographic location and host plant. The maximum spectrum of melleolides (7-8 compounds) was found in isolates of A. borealis, A. gallica, A. sinapina, A. ostoyae. In submerged culture, the maximum accumulation of melleolides varied from 2 up to 239 mg l(-1). A mixture of melleolide D and melledonal C (1:1) synthesized by the most productive strain A. mellea Cr2-17 was first found to have a phytotoxic action on the growth parameters of the callus culture Populus balsamifera and 10-day-old conifer seedlings. A 0.5% concentration of melleolides caused a credible decrease of P. balsamifera callus raw biomass; a decrease of the viability of Larix sibirica and, which is especially significant, Pinus sylvestris seedlings; inhibition of stem and root growth processes; dechromation of foliage; loss of turgor. The occurrence of a broad range of melleolides in the metabolome profile and two common compounds in all investigated strains, with a phytotoxic action at their sufficiently high concentration, enables considering the synthesis of melleolides by Armillaria fungi as one of the possible mechanisms of their pathogenicity efficiently realized in strains characterized by overproduction of melleolides under natural conditions.

WOS

Держатели документа:
Russian Acad Sci, GK Skryabin Inst Biochem & Physiol Microorganisms, FRC Pushchino Ctr Biol Res, 5 Prosp Nauki, Pushchino 142290, Moscow Region, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, FRC KSC, Siberian Branch, 50 Akad Gorodok Str, Krasnoyarsk 660036, Russia.
FSBEIHE MF Reshetnev Siberian State Univ Sci & Te, 82 Prosp Mira, Krasnoyarsk 660037, Russia.
FSBEIHE Krasnoyarsk State Agr Univ, 90 Prosp Mira, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Antipova, Tatyana, V; Zhelifonova, Valentina P.; Litovka, Yulia A.; Pavlov, Igor N.; Baskunov, Boris P.; Kokh, Zhanna A.; Makolova, Polina, V; Timofeev, Anton A.; Kozlovsky, Anatoly G.