Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ G. I. Antonov [и др.] // Russ. J. For. Sci. - 2018. - Is. 1. - С. 56-64, DOI 10.7868/S0024114818010059 . - ISSN 0024-1148
Аннотация: The effect from addition of sawdust pulp substrates together with micro-doses of various mineral fertilizers in soil was assessed from transformation of soil nitrogen under Scots pine and Siberian spruce saplings. Readily hydrolysable nitrogen exceeded hardly hydrolysable nitrogen under most of variations of fertilizers used when sawdust pulp substrates has been added. This point to high biological activity of soil and optimal environment for microbial enzymatic biodegradation of sawdust pulp substrates under the saplings. We found direct effect of ammonia nitrogen in soil of nursery on enzymatic nitrogen in needles of pine and spruce. Addition of sawdust pulp substrates significantly increased total and enzymatic nitrogen in the sapling needles. Concentration of total nitrogen in the needles of pine and spruce rose up to 21.7 and 27.6 mg per g of oven-dry substance, when grown on sawdust pulp substrates. On the contrary, the total nitrogen concentration in the needles of control species was less than 10.3 and 9.1 mg per g of oven-dry matter, respectively. Maximal concentrations of enzymatic nitrogen in pine needles (12.5–14.6 mg per g of oven-dry matter), composing 67.5–75% of the total nitrogen were found when carbamide, ammonia sulfate, German saltpeter and diammophoska had been added to sawdust pulp substrates. Maximal concentrations of enzymatic nitrogen in spruce needles (17.9–19.0 mg per g of oven-dry matter), composing 71.4–80.5% of the total nitrogen were found when German saltpeter, ammonia sulfate and carbamide had been added. Thus, sawdust pulp substrates with micro-doses of ammonia sulfate, carbamide, diammophoska and German saltpeter were most effective for nitrogen accumulation in the needles. © 2018, Izdatel'stvo Nauka. All rights reserved.

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Держатели документа:
Forest Institute, Siberian Branch of the Russian Academy of Sciences, Academgorodok 50 bldg. 28, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Antonov, G. I.; Sorokin, N. D.; Barchenkov, A. P.; Kondakova, O. E.

/ Y. A. Litovka [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2017. - Is. 1. - С. 145-150, DOI 10.14258/jcprm.2017011577 . - ISSN 1029-5151
Аннотация: Presents the results of a research of wood-destroying properties Porodaedalea niemelaei M. Fischer and Trichoderma atroviride Bissett. The strains were isolated in pure culture from the fruiting body and larch wood (Larix gmelinii (Rupr.) Rupr.) in permafrost. Studied phenol oxidase activity and biodegradation of waste wood at their solid-state fermentation. Under laboratory conditions, fungi colonize deciduous and coniferous substrates in monoculture and co-culture. Bioconversion depends on the enzymatic activity of strains and chemical characteristics of the growth substrate. Maximum of wooddestroying activity observed in strain PnB-13 Porodaedalea niemelaei on sawdust of aspen. The loss of weight of the substrate was 39%; polysaccharide content decreased to 1,6 times in comparison with the initial substrate; lignin - in 1.2 times. The concentration of water-soluble substances increased 1,5 times. P. niemelaei and T. atroviride produce extracellular oxidase of lignolytic complex in the solid-phase cultivation of plant residues. Enzyme activity was 0,15-0,22 u/g.s on sawdust of larch and 0,14-0,18 u/g.s on sawdust of aspen, which is comparable to the phenol oxidase activity of other xylotrophic basidiomycetes (Trametes versicolor, Armillaria mellea s.l., Heterobasidion parviporum).

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Держатели документа:
Siberian State Aerospace University. Academician M.F. Reshetnev, pr. Mira, 82, Krasnoyarsk, Russian Federation
Institute of Forest. V.N. Sukachev, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/28, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Litvinova, E. A.; Chuprova, N. A.

/ Y. A. Litovka [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2018. - Is. 1. - С. 193-199, DOI 10.14258/jcprm.2018012729 . - ISSN 1029-5151
Аннотация: Presents the results of a research of wood-destroying properties of the basidiomycete Fomitopsis pinicola (Sw.) P. Karst. The strains were isolated in pure culture from fruiting bodies that grew on the Abies sibirica Ledeb, Pinus sylvestris Ledeb, Pinus sibirica Du Tour and Larix sibirica Ledeb in the forests of the Krasnoyarsk Territory (56°09'-56°22' N; 92°10'- 92°58' E). The cultures screened for growth parameters and the dynamics of enzymatic activity during cultivation on cellulose-containing media. The radial growth rate of Siberian strains on the agar medium and plant substrates is 1,1-3,3 mm / day; the growth coefficient is 10-42. All studied cultures synthesize extracellular cellulolytic enzymes that hydrolyze the sodium salt of carboxymethylcellulose. The enzymatic activity is in the range 0,05-0,39 U / ml with a maximum of 7-9 days. The most active strain of Fp5-15 F. pinicola used for bioconversion of coniferous plant substrates under solid-phase culture conditions. The maximum wood-destroying activity observed on pine and larch wood - the mass loss of substrates was 16 and 20%, respectively. On Pinus sylvestris sawdust, the total content of polysaccharides decreased by 1,2 times due to the fermentolysis of the hardly hydrolyzable fraction (a decrease of 1,4 times); the content of lignin substances did not change significantly. On the sawdust of Larix sibirica, the amount of polysaccharides decreased by 1,3 times in comparison with the initial substrate; lig-nin - in 1,2 times. © 2018 Altai State University. All rights reserved.

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Держатели документа:
Forest Institute, V.N. Sukachev Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 50-28, Krasnoyarsk, Russian Federation
Siberian State University of Science and Technology, Academician M.F. Reshetnev, Mira ave., 82, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Gazizulina, A. V.; Chuprova, N. A.

/ D. Schepaschenko [et al.] // Forests. - 2018. - Vol. 9, Is. 6, DOI 10.3390/f9060312 . - ISSN 1999-4907
Аннотация: Biomass structure is an important feature of terrestrial vegetation. The parameters of forest biomass structure are important for forest monitoring, biomass modelling and the optimal utilization and management of forests. In this paper, we used the most comprehensive database of sample plots available to build a set of multi-dimensional regression models that describe the proportion of different live biomass fractions (i.e., the stem, branches, foliage, roots) of forest stands as a function of average stand age, density (relative stocking) and site quality for forests of the major tree species of northern Eurasia. Bootstrapping was used to determine the accuracy of the estimates and also provides the associated uncertainties in these estimates. The species-specific mean percentage errors were then calculated between the sample plot data and the model estimates, resulting in overall relative errors in the regression model of -0.6%, -1.0% and 11.6% for biomass conversion and expansion factor (BCEF), biomass expansion factor (BEF), and root-to-shoot ratio respectively. The equations were then applied to data obtained from the Russian State Forest Register (SFR) and a map of forest cover to produce spatially distributed estimators of biomass conversion and expansion factors and root-to-shoot ratios for Russian forests. The equations and the resulting maps can be used to convert growing stock volume to the components of both above-ground and below-ground live biomass. The new live biomass conversion factors can be used in different applications, in particular to substitute those that are currently used by Russia in national reporting to the UNFCCC (United Nations Framework Convention on Climate Change) and the FAO FRA (Food and Agriculture Organization's Forest Resource Assessment), among others. © 2018 by the authors.

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Держатели документа:
International Institute for Applied Systems Analysis, Laxenburg, Austria
Forestry Faculty, Bauman Moscow State Technical University, Mytischi, Russian Federation
School of Mathematics and Statistics, University of Canterbury, Christchurch, New Zealand
Institute of Forest Siberian Branch Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Education and Research Institute of Forestry and Park Gardening, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
Institute of Numerical Mathematics of Russian Academy of Sciences, Ul. Gubkina 8, Moscow, Russian Federation
The Earth Science Museum, M.V. Lomonosov Moscow State University, 1 Leninskiye Gory, GSP-1, Moscow, Russian Federation

Доп.точки доступа:
Schepaschenko, D.; Moltchanova, E.; Shvidenko, A.; Blyshchyk, V.; Dmitriev, E.; Martynenko, O.; See, L.; Kraxner, F.

/ Zh. A. Koh, Yu. A. Litovka, R. Kh. Enazarov [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 359-369, DOI 10.14258/JCPRM.2020048392 . - ISSN 1029-5151
   Перевод заглавия: Биотехнологические аспекты утилизации послеэкстракционной биомассы и клеточной культуры orthilia secunda (L.) house базидиальными грибами
Аннотация: The possibility of bioconversion after the extraction residue of the medicinal plant Orthilia secunda (initial plant biomass and callus after extraction of biologically active substances) by fast growing strains of basidiomycetes Tv2-16K Trametes versicolor and Pe-17T Pleurotus eryngii was studied. The main target products are: a mycelial-plant product with a reduced content of the indigestible fraction and enriched in protein and full-fledged fruit bodies. Fungi colonize plant waste with a radial growth rate of 2.0-2.3 mm / day and a growth rate of 65-77 units. Phenol oxidase activity is 0.7-1.2 units/g·s, depending on the type after the extraction residue. In substrates after biodegradation, the proportion of difficult hydrolysable polysaccharides, easily hydrolysable polysaccharides, and lignin is on average 1.6 times lower than in the original substrate. The content of protein and extractive substances is significantly higher, especially after the extraction residue of O. secunda callus under the influence of the T. versicolor strain Tv2-16K: 12.8 and 24.3%, respectively, of the mass of dry substrate. Fruit bodies of P. eryngii strain Pe-17T were obtained on various compositions of plant substrates. The maximum fruit formation was noted on four-component substrates containing two types after extraction residues of O. secunda, wheat bran, birch or aspen sawdust: the average weight of fruit bodies from one block was 230-236 g; biological efficiency - 46-47.2%. © 2020 Altai State University. All rights reserved.

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Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation

Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Enazarov, R. Kh.; Makolova, P. V.; Shimova, Yu. S.; Pochekutov, I. S.; Pavlov, I. N.

/ Yu. A. Litovka, I. N. Pavlov, P. V. Makolova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 371-383, DOI 10.14258/JCPRM.2020048396 . - ISSN 1029-5151
   Перевод заглавия: Биотехнологический потенциал сибирских штаммов базидиальных грибов - продуцентов ферментов лигноцеллюлазного действия
Аннотация: The results of a study of the wood-destroying properties of Siberian strains of xylotrophic basidiomycetes (Armillaria, Ganoderma, Fomitopsis, Heterobasidion and Porodaedalea) are presented. The growth parameters and enzymatic activity of the strains were determined during solid-phase and deep cultivation. Fomitopsis pinicola and Ganoderma lucidum are fast-growing fungi on cellulose-, tannin-containing nutrient media, coniferous and deciduous plant substrates (source and hydrodynamically activated). The growth coefficient on media with tannin and Na-carboxymethyl cellulose is more than 45; on plant substrates - more than 30. The strains exhibit a different growth reaction to the preliminary activation of birch sawdust (growth acceleration / growth slowdown / indifference). The maximum wood-destroying activity on wood of A. sibirica noted for the fungi A. borealis, G. tsugae, G. lucidum, F. pinicola, and P. niemelaei. The decrease in substrate mass was 8-11%; the amount of polysaccharides decreased on average 1.4 times mainly due to the fermentolysis of hard-hydrolyzable polysaccharides. High enzymatic activity of fungi observed during solid-phase and deep cultivation with an inducer. The maximum activity of phenol oxidase is characteristic of G. tsugae (1.21 units/g·s); carboxymethyl cellulase - for F.pinicola and G. lucidum strains (11.8 and 10.3 units/ml, respectively); xylanases - for H. abietinum (3.8 u/ml). The maximum accumulation of extracellular protein observed in F. pinicola (0.89 mg/ml). According to the totality of rapid test indicators, quantitative determination of enzymatic activity, growth parameters on lignocellulosic substrates and the degree of wood bioconversion, the most promising producer of lignolytic enzymes in vitro is the Gl4-16A Ganoderma lucidum strain; cellulolytic enzymes - strain Fp6-17 Fomitopsis pinicola. © 2020 Altai State University. All rights reserved.

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Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Institute of Physics L.V. Kirensky, SB, RAS, Akademgorodok, 50/43, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Litovka, Yu. A.; Pavlov, I. N.; Makolova, P. V.; Timofeev, A. A.; Litvinova, E. A.; Vasil'Eva, A. A.; Shabanov, A. V.

/ O. O. Mamaeva, E. V. Isaeva, S. R. Loskutov, M. A. Plyashechnik // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 1. - С. 277-285, DOI 10.14258/JCPRM.2021018851 . - ISSN 1029-5151
Аннотация: Today, plant waste, including fallen poplar leaves (litter), are a promising raw material for the production of useful prod-ucts using bioconversion, such as protein feed additives. The aim of this study was to study the component composition of the products obtained as a result of the bioconversion of leaf litter. The strain PP-3.2 Pleurotus pulmonarius (Fr.) Quel. In the process of substrate conversion, strain PP-3.2 primarily utilizes extractives and easily hydrolyzable polysaccharides, the amount of which decreases by 44 and 36%, respectively. The total content of polysaccharides is reduced by 20%, lignin substances-9.4%. The loss of substrate mass based on leaf litter was 23%. The product obtained after cultivation contains 28% protein. Analysis of the amino acid composition of the protein showed a high rate of phenylalanine with tyrosine (115), threonine (117.5), valine (110) and isoleucine (105%). Also, the biodegradation product has a high content of glutamic and aspartic acids (11.2 and 25.3% of the total amino acids, respectively). The levels of cadmium, lead and copper revealed as a result of studies do not exceed the maximum permissible concentrations established by the veterinary department. At the same time, there is a high content of iron (166.0 Eg/kg) and zinc (256.7 mg/kg) in the feed product. The digestibility of the product is 54%, the content of nucleic substances is up to 0.3%. Thus, the results obtained show the possibility of using the product of the conversion of poplar fallen leaves as a protein feed additive. © 2021 Altai State University. All rights reserved.

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Держатели документа:
Reshetnev Siberian State University of Science and Technology, pr. Krasnoyarskii Rabochii, 31, Krasnoyarsk, 660037, Russian Federation
Sukachev Institute of Forest SB RAS, Federal Research Center «Krasnoyarsk Scientific Center SB RAS», Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Mamaeva, O. O.; Isaeva, E. V.; Loskutov, S. R.; Plyashechnik, M. A.

/ V. S. Fedorov, T. V. Ryazanova, Y. A. Litovka [и др.] // J. Sib. Fed. Univ.-Chem. - 2022. - Vol. 15, Is. 1. - С. 90-101, DOI 10.17516/1998-2836-0274. - Cited References:30. - The research was conducted under the governmental task issued by the Ministry of Education and Science of Russia for the "Technology and Equipment for the Chemical Processing of the Plant Biomass Material" project by the Plant Material Deep Conversion Laboratory (topic number FEFE-2020-0016) . . - ISSN 1998-2836. - ISSN 2313-6049РУБ Chemistry, Multidisciplinary

Аннотация: The results of a study on the use of sawdust of common pine Pinus sylvestris L. with their preliminary activation in a cavitation water-impact dispersant for 25 minutes as a substrate for cultivating the G14-16A strain of Ganoderma lucid= are presented. it was found that the G14-16A strain of Ganoderma lucidum colonizes the substrate more efficiently with the introduction of (NH4)(2)SO4 and Na2HPO4 salts into its composition. At the same time, the average growth rate of basidiomycete on the substrate without the introduction of salts was 1.39 +/- 0.53 mm/day, and on the substrate with nitrogen salts - 3.22 +/- 0.48 mm/day, and complete fouling of the substrate occurred on day 13. It was found that during the cultivation of the G14-16A strain of Ganoderma lucidum on a substrate with nitrogen salts, the content of extractive substances in it increases from 4.37 % to 6.32 %, where more than 65 % is accounted for by substances extracted with water. There is a decrease in the content of difficult-to-hydrolyze polysaccharides by 13.3 %, and an increase in the non-hydrolyzable part by 2.4 %, which is 6.8 % per organic mass. The content of easily hydrolyzable polysaccharides (LGA) decreases by 2.4 %, but this change is not significant in terms of organic mass and the share of LGA both before and after bioconversion is about 15.7 %. The obtained data can expand the field of use of sawdust, which is a promising direction, both in the chemical and pharmaceutical industries.

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Держатели документа:
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, Krasnoyarsk, Russia.

Доп.точки доступа:
Fedorov, Vladimir S.; Ryazanova, Tatyana, V; Litovka, Yulia A.; Pavlova, Igor N.; Litvinova, Ekaterina A.; Petrunina, Elena A.; Loskutov, Sergey R.; Ermolin, Vladimir N.; Bayandin, Mikhail A.; Ministry of Education and Science of Russia for the "Technology and Equipment for the Chemical Processing of the Plant Biomass Material" project by the Plant Material Deep Conversion Laboratory [FEFE-2020-0016]