/ N. Gentsch [et al.] // Eur. J. Soil Sci. - 2015. - Vol. 66, Is. 4. - P722-734, DOI 10.1111/ejss.12269
. - ISSN 1351-0754
Аннотация: Permafrost degradation may cause strong feedbacks of arctic ecosystems to global warming, but this will depend on if, and to what extent, organic matter (OM) is protected against biodegradation by mechanisms other than freezing and anoxia. Here, we report on the amount, chemical composition and bioavailability of particulate (POM) and mineral-associated OM (MOM) in permafrost soils of the East Siberian Arctic. The average total organic carbon (OC) stock across all soils was 24.0 ± 6.7 kg m-2 within 100 cm soil depth. Density fractionation (density cut-off 1.6 g cm-3) revealed that 54 ± 16% of the total soil OC and 64 ± 18% of OC in subsoil horizons was bound to minerals. As well as sorption of OM to clay-sized minerals (R2 = 0.80; P 0.01), co-precipitation of OM with hydrolyzable metals may also transfer carbon into the mineral-bound fraction. Carbon:nitrogen ratios, stable carbon and nitrogen isotopes, 13C-NMR and X-ray photoelectron spectroscopy showed that OM is transformed in permafrost soils, which is a prerequisite for the formation of mineral-organic associations. Mineral-associated OM in deeper soil was enriched in 13C and 15N, and had narrow C:N and large alkyl C:(O-/N-alkyl C) ratios, indicating an advanced stage of decomposition. Despite being up to several thousands of years old, when incubated under favourable conditions (60% water-holding capacity, 15°C, adequate nutrients, 90 days), only 1.5-5% of the mineral-associated OC was released as COinf2/inf. In the topsoils, POM had the largest mineralization but was even less bioavailable than the MOM in subsoil horizons. Our results suggest that the formation of mineral-organic associations acts as an important additional factor in the stabilization of OM in permafrost soils. Although the majority of MOM was not prone to decomposition under favourable conditions, mineral-organic associations host a readily accessible carbon fraction, which may actively participate in ecosystem carbon exchange. © 2015 British Society of Soil Science.
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Держатели документа:
Institut für Bodenkunde, Leibniz Universität Hannover, Herrenhäuser Straße 2, Hannovern, Germany
VN Sukachev Institute of Forest, Akademgorodok 50, Krasnoyarsk, Russian Federation
Division of Terrestrial Ecosystem Research, Department of Microbiology and Ecosystem Science, University of Vienna, Althanstr. 14, Vienna, Austria
Austrian Polar Research Institute, Althanstra?e 14, Vienna, Austria
Department of Earth Sciences, University of Gothenburg, Guldhedsgatan 5A, Gothenburg, Sweden
Department of Natural Resources and the Environment, University of New Hampshire, Durham, NH, United States
Department of Ecogenomics and Systems Biology, University of Vienna, Althanstr. 14, Vienna, Austria
Department of Biology, Centre for Geobiology, University of Bergen, Postboks 7803, Bergen, Norway
Department of Bioscience, Norway and Center for Geomicrobiology, Aarhus University, Ny Munkegade 116, Aarhus C, Denmark
Department of Ecosystem Biology, University of South Bohemia, Branisovska 1760, Ceske Budejovice, Czech Republic
Central SiberianBotanical Garden, Siberian Branch of the Russian Academy of Sciences, Zolotodolinskya Street 101, Novosibirsk, Russian Federation
Lehrstuhl fur Bodenkunde, Technische Universitat Munchen, Emil-Ramann Strasse 2, Freising, Germany
Thunen Institute of Climate Smart Agriculture, Bundesallee 50, Braunschweig, Germany
Доп.точки доступа:
Gentsch, N.; Mikutta, R.; Shibistova, O.; Wild, B.; Schnecker, J.; Richter, A.; Urich, T.; Gittel, A.; Santruckova, H.; Barta, J.; Lashchinskiy, N.; Mueller, C.W.; Fuß, R.; Guggenberger, G.
Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН
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Properties and bioavailability of particulate and mineral-associated organic matter in Arctic permafrost soils, Lower Kolyma Region, Russia
Post fire organic matter biodegradation in permafrost soils: Case study after experimental heating of mineral horizons
/ O. V. Masyagina, I. V. Tokareva, A. S. Prokushkin // Sci. Total Environ. - 2016. - Vol. 573. - P1255-1264, DOI 10.1016/j.scitotenv.2016.04.195. - Cited References:52. - The reported study was partially supported by the Russian Science Foundation (14-24-00113).
. - ISSN 0048-9697. - ISSN 1879-1026
РУБ Environmental Sciences
Аннотация: Periodical ground fires of high frequency in permafrost forest ecosystems of Siberia (Russian Federation) are essential factors determining quantitative and qualitative parameters of permafrost soil organic matter. Specific changes in physical and chemical parameters and microbial activity of permafrost soil mineral horizons of northern taiga larch stands were revealed after heating at high temperatures (150-500 degrees C) used for imitation of different burn intensities. Burning at 150-200 degrees C resulted in decreasing of soil pH, whilst heating at 300-500 degrees C caused increase of pH compare to unheated soils. Water-soluble organic carbon concentration in permafrost soils heated at 150-200 degrees C was much higher than that of unheated soils. All these changes determined soil microbial activity in heated soils. In particular, in soils heated at 300-500 degrees C there was momentary stimulating effect on substrate-induced respiration registered and on basal respiration values in soils burned at 150 degrees C and 300-400 degrees C. Four-month laboratory incubation of permafrost soils heated at different temperatures showed stimulation of microbial activity in first several days after inoculation due to high substrate availability after heating. Then soon after that soil microbial community started to be depleted on substrate because of decreasing water-soluble organic carbon, C and N content and it continued to the end of incubation. (C) 2016 Elsevier B.V. All rights reserved.
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Держатели документа:
VN Sukachev Inst Forest SB RAS, 50-28 Akad Gorodok, Krasnoyarsk 660036, Russia.
Доп.точки доступа:
Masyagina, O. V.; Tokareva, I. V.; Prokushkin, A. S.; Russian Science Foundation [14-24-00113]
Рубрики:
BOREAL FOREST
MICROBIAL ACTIVITY
WILDFIRE
CARBON
NITROGEN
PH
Кл.слова (ненормированные):
Ground fires -- Soil microorganisms -- Permafrost -- Soil organic carbon -- Heating temperature
BOREAL FOREST
MICROBIAL ACTIVITY
WILDFIRE
CARBON
NITROGEN
PH
Кл.слова (ненормированные):
Ground fires -- Soil microorganisms -- Permafrost -- Soil organic carbon -- Heating temperature
Аннотация: Periodical ground fires of high frequency in permafrost forest ecosystems of Siberia (Russian Federation) are essential factors determining quantitative and qualitative parameters of permafrost soil organic matter. Specific changes in physical and chemical parameters and microbial activity of permafrost soil mineral horizons of northern taiga larch stands were revealed after heating at high temperatures (150-500 degrees C) used for imitation of different burn intensities. Burning at 150-200 degrees C resulted in decreasing of soil pH, whilst heating at 300-500 degrees C caused increase of pH compare to unheated soils. Water-soluble organic carbon concentration in permafrost soils heated at 150-200 degrees C was much higher than that of unheated soils. All these changes determined soil microbial activity in heated soils. In particular, in soils heated at 300-500 degrees C there was momentary stimulating effect on substrate-induced respiration registered and on basal respiration values in soils burned at 150 degrees C and 300-400 degrees C. Four-month laboratory incubation of permafrost soils heated at different temperatures showed stimulation of microbial activity in first several days after inoculation due to high substrate availability after heating. Then soon after that soil microbial community started to be depleted on substrate because of decreasing water-soluble organic carbon, C and N content and it continued to the end of incubation. (C) 2016 Elsevier B.V. All rights reserved.
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Держатели документа:
VN Sukachev Inst Forest SB RAS, 50-28 Akad Gorodok, Krasnoyarsk 660036, Russia.
Доп.точки доступа:
Masyagina, O. V.; Tokareva, I. V.; Prokushkin, A. S.; Russian Science Foundation [14-24-00113]
Optimisation of silviculture using bioconversion of sawdust pulp in Krasnoyarsk forest-steppe
/ G. I. Antonov [и др.] // Russ. J. For. Sci. - 2018. - Is. 1. - С. 56-64, DOI 10.7868/S0024114818010059
. - ISSN 0024-1148
Кл.слова (ненормированные):
Composted fertilizer -- Enzymatic nitrogen -- Experimental nursery -- Microbial bioconversion -- Nutritional substrate -- Sawdust -- Scots pine -- Siberian spruce -- Soil nitrogen
Аннотация: The effect from addition of sawdust pulp substrates together with micro-doses of various mineral fertilizers in soil was assessed from transformation of soil nitrogen under Scots pine and Siberian spruce saplings. Readily hydrolysable nitrogen exceeded hardly hydrolysable nitrogen under most of variations of fertilizers used when sawdust pulp substrates has been added. This point to high biological activity of soil and optimal environment for microbial enzymatic biodegradation of sawdust pulp substrates under the saplings. We found direct effect of ammonia nitrogen in soil of nursery on enzymatic nitrogen in needles of pine and spruce. Addition of sawdust pulp substrates significantly increased total and enzymatic nitrogen in the sapling needles. Concentration of total nitrogen in the needles of pine and spruce rose up to 21.7 and 27.6 mg per g of oven-dry substance, when grown on sawdust pulp substrates. On the contrary, the total nitrogen concentration in the needles of control species was less than 10.3 and 9.1 mg per g of oven-dry matter, respectively. Maximal concentrations of enzymatic nitrogen in pine needles (12.5–14.6 mg per g of oven-dry matter), composing 67.5–75% of the total nitrogen were found when carbamide, ammonia sulfate, German saltpeter and diammophoska had been added to sawdust pulp substrates. Maximal concentrations of enzymatic nitrogen in spruce needles (17.9–19.0 mg per g of oven-dry matter), composing 71.4–80.5% of the total nitrogen were found when German saltpeter, ammonia sulfate and carbamide had been added. Thus, sawdust pulp substrates with micro-doses of ammonia sulfate, carbamide, diammophoska and German saltpeter were most effective for nitrogen accumulation in the needles. © 2018, Izdatel'stvo Nauka. All rights reserved.
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Держатели документа:
Forest Institute, Siberian Branch of the Russian Academy of Sciences, Academgorodok 50 bldg. 28, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Antonov, G. I.; Sorokin, N. D.; Barchenkov, A. P.; Kondakova, O. E.
Кл.слова (ненормированные):
Composted fertilizer -- Enzymatic nitrogen -- Experimental nursery -- Microbial bioconversion -- Nutritional substrate -- Sawdust -- Scots pine -- Siberian spruce -- Soil nitrogen
Аннотация: The effect from addition of sawdust pulp substrates together with micro-doses of various mineral fertilizers in soil was assessed from transformation of soil nitrogen under Scots pine and Siberian spruce saplings. Readily hydrolysable nitrogen exceeded hardly hydrolysable nitrogen under most of variations of fertilizers used when sawdust pulp substrates has been added. This point to high biological activity of soil and optimal environment for microbial enzymatic biodegradation of sawdust pulp substrates under the saplings. We found direct effect of ammonia nitrogen in soil of nursery on enzymatic nitrogen in needles of pine and spruce. Addition of sawdust pulp substrates significantly increased total and enzymatic nitrogen in the sapling needles. Concentration of total nitrogen in the needles of pine and spruce rose up to 21.7 and 27.6 mg per g of oven-dry substance, when grown on sawdust pulp substrates. On the contrary, the total nitrogen concentration in the needles of control species was less than 10.3 and 9.1 mg per g of oven-dry matter, respectively. Maximal concentrations of enzymatic nitrogen in pine needles (12.5–14.6 mg per g of oven-dry matter), composing 67.5–75% of the total nitrogen were found when carbamide, ammonia sulfate, German saltpeter and diammophoska had been added to sawdust pulp substrates. Maximal concentrations of enzymatic nitrogen in spruce needles (17.9–19.0 mg per g of oven-dry matter), composing 71.4–80.5% of the total nitrogen were found when German saltpeter, ammonia sulfate and carbamide had been added. Thus, sawdust pulp substrates with micro-doses of ammonia sulfate, carbamide, diammophoska and German saltpeter were most effective for nitrogen accumulation in the needles. © 2018, Izdatel'stvo Nauka. All rights reserved.
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Держатели документа:
Forest Institute, Siberian Branch of the Russian Academy of Sciences, Academgorodok 50 bldg. 28, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Antonov, G. I.; Sorokin, N. D.; Barchenkov, A. P.; Kondakova, O. E.
Wood-destroying properties of porodaedalea niemela m. Fischer and trichoderma atroviride bissett from permafrost area
/ Y. A. Litovka [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2017. - Is. 1. - С. 145-150, DOI 10.14258/jcprm.2017011577
. - ISSN 1029-5151
Кл.слова (ненормированные):
Biodegradation -- Lignocarbohydrate complex -- Phenol oxidase activity -- Porodaedalea niemelaei -- Solid-state cultivation -- Trichoderma atroviride -- Waste wood
Аннотация: Presents the results of a research of wood-destroying properties Porodaedalea niemelaei M. Fischer and Trichoderma atroviride Bissett. The strains were isolated in pure culture from the fruiting body and larch wood (Larix gmelinii (Rupr.) Rupr.) in permafrost. Studied phenol oxidase activity and biodegradation of waste wood at their solid-state fermentation. Under laboratory conditions, fungi colonize deciduous and coniferous substrates in monoculture and co-culture. Bioconversion depends on the enzymatic activity of strains and chemical characteristics of the growth substrate. Maximum of wooddestroying activity observed in strain PnB-13 Porodaedalea niemelaei on sawdust of aspen. The loss of weight of the substrate was 39%; polysaccharide content decreased to 1,6 times in comparison with the initial substrate; lignin - in 1.2 times. The concentration of water-soluble substances increased 1,5 times. P. niemelaei and T. atroviride produce extracellular oxidase of lignolytic complex in the solid-phase cultivation of plant residues. Enzyme activity was 0,15-0,22 u/g.s on sawdust of larch and 0,14-0,18 u/g.s on sawdust of aspen, which is comparable to the phenol oxidase activity of other xylotrophic basidiomycetes (Trametes versicolor, Armillaria mellea s.l., Heterobasidion parviporum).
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Держатели документа:
Siberian State Aerospace University. Academician M.F. Reshetnev, pr. Mira, 82, Krasnoyarsk, Russian Federation
Institute of Forest. V.N. Sukachev, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/28, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Litvinova, E. A.; Chuprova, N. A.
Кл.слова (ненормированные):
Biodegradation -- Lignocarbohydrate complex -- Phenol oxidase activity -- Porodaedalea niemelaei -- Solid-state cultivation -- Trichoderma atroviride -- Waste wood
Аннотация: Presents the results of a research of wood-destroying properties Porodaedalea niemelaei M. Fischer and Trichoderma atroviride Bissett. The strains were isolated in pure culture from the fruiting body and larch wood (Larix gmelinii (Rupr.) Rupr.) in permafrost. Studied phenol oxidase activity and biodegradation of waste wood at their solid-state fermentation. Under laboratory conditions, fungi colonize deciduous and coniferous substrates in monoculture and co-culture. Bioconversion depends on the enzymatic activity of strains and chemical characteristics of the growth substrate. Maximum of wooddestroying activity observed in strain PnB-13 Porodaedalea niemelaei on sawdust of aspen. The loss of weight of the substrate was 39%; polysaccharide content decreased to 1,6 times in comparison with the initial substrate; lignin - in 1.2 times. The concentration of water-soluble substances increased 1,5 times. P. niemelaei and T. atroviride produce extracellular oxidase of lignolytic complex in the solid-phase cultivation of plant residues. Enzyme activity was 0,15-0,22 u/g.s on sawdust of larch and 0,14-0,18 u/g.s on sawdust of aspen, which is comparable to the phenol oxidase activity of other xylotrophic basidiomycetes (Trametes versicolor, Armillaria mellea s.l., Heterobasidion parviporum).
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Держатели документа:
Siberian State Aerospace University. Academician M.F. Reshetnev, pr. Mira, 82, Krasnoyarsk, Russian Federation
Institute of Forest. V.N. Sukachev, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/28, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Litvinova, E. A.; Chuprova, N. A.
Wood-destroying properties of Fomitopsis Pinicola (SW.) P. Karst. From middle Siberia
/ Y. A. Litovka [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2018. - Is. 1. - С. 193-199, DOI 10.14258/jcprm.2018012729
. - ISSN 1029-5151
Кл.слова (ненормированные):
Biodegradation -- Cellulase activity -- Fomitopsis pinicola -- Lignocarbohydrate complex -- Solid-state cultivation -- Waste wood -- Wood-destroying (xylotrophic) fungi
Аннотация: Presents the results of a research of wood-destroying properties of the basidiomycete Fomitopsis pinicola (Sw.) P. Karst. The strains were isolated in pure culture from fruiting bodies that grew on the Abies sibirica Ledeb, Pinus sylvestris Ledeb, Pinus sibirica Du Tour and Larix sibirica Ledeb in the forests of the Krasnoyarsk Territory (56°09'-56°22' N; 92°10'- 92°58' E). The cultures screened for growth parameters and the dynamics of enzymatic activity during cultivation on cellulose-containing media. The radial growth rate of Siberian strains on the agar medium and plant substrates is 1,1-3,3 mm / day; the growth coefficient is 10-42. All studied cultures synthesize extracellular cellulolytic enzymes that hydrolyze the sodium salt of carboxymethylcellulose. The enzymatic activity is in the range 0,05-0,39 U / ml with a maximum of 7-9 days. The most active strain of Fp5-15 F. pinicola used for bioconversion of coniferous plant substrates under solid-phase culture conditions. The maximum wood-destroying activity observed on pine and larch wood - the mass loss of substrates was 16 and 20%, respectively. On Pinus sylvestris sawdust, the total content of polysaccharides decreased by 1,2 times due to the fermentolysis of the hardly hydrolyzable fraction (a decrease of 1,4 times); the content of lignin substances did not change significantly. On the sawdust of Larix sibirica, the amount of polysaccharides decreased by 1,3 times in comparison with the initial substrate; lig-nin - in 1,2 times. © 2018 Altai State University. All rights reserved.
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Держатели документа:
Forest Institute, V.N. Sukachev Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 50-28, Krasnoyarsk, Russian Federation
Siberian State University of Science and Technology, Academician M.F. Reshetnev, Mira ave., 82, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Gazizulina, A. V.; Chuprova, N. A.
Кл.слова (ненормированные):
Biodegradation -- Cellulase activity -- Fomitopsis pinicola -- Lignocarbohydrate complex -- Solid-state cultivation -- Waste wood -- Wood-destroying (xylotrophic) fungi
Аннотация: Presents the results of a research of wood-destroying properties of the basidiomycete Fomitopsis pinicola (Sw.) P. Karst. The strains were isolated in pure culture from fruiting bodies that grew on the Abies sibirica Ledeb, Pinus sylvestris Ledeb, Pinus sibirica Du Tour and Larix sibirica Ledeb in the forests of the Krasnoyarsk Territory (56°09'-56°22' N; 92°10'- 92°58' E). The cultures screened for growth parameters and the dynamics of enzymatic activity during cultivation on cellulose-containing media. The radial growth rate of Siberian strains on the agar medium and plant substrates is 1,1-3,3 mm / day; the growth coefficient is 10-42. All studied cultures synthesize extracellular cellulolytic enzymes that hydrolyze the sodium salt of carboxymethylcellulose. The enzymatic activity is in the range 0,05-0,39 U / ml with a maximum of 7-9 days. The most active strain of Fp5-15 F. pinicola used for bioconversion of coniferous plant substrates under solid-phase culture conditions. The maximum wood-destroying activity observed on pine and larch wood - the mass loss of substrates was 16 and 20%, respectively. On Pinus sylvestris sawdust, the total content of polysaccharides decreased by 1,2 times due to the fermentolysis of the hardly hydrolyzable fraction (a decrease of 1,4 times); the content of lignin substances did not change significantly. On the sawdust of Larix sibirica, the amount of polysaccharides decreased by 1,3 times in comparison with the initial substrate; lig-nin - in 1,2 times. © 2018 Altai State University. All rights reserved.
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Держатели документа:
Forest Institute, V.N. Sukachev Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 50-28, Krasnoyarsk, Russian Federation
Siberian State University of Science and Technology, Academician M.F. Reshetnev, Mira ave., 82, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Gazizulina, A. V.; Chuprova, N. A.
Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
/ S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180
. - ISSN 0045-6535
Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
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Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation
Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.
Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation
Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.
Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards "green" bioplastics
/ S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180. - Cited References:101. - This study (polymer synthesis and investigation) was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning", and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (investigation of polymer degradation in soils of Evenkia).
. - ISSN 0045-6535. - ISSN 1879-1298
РУБ Environmental Sciences
Кл.слова (ненормированные):
Siberian cryogenic soils -- P(3HB) bioplastic -- metabolic activity -- structure of microbial community -- P(3HB)-degrading strains -- P(3HB) -- properties
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, VN Sukachev Inst Forest, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Melnikov Permafrost Inst, 36 Merzlotnaya St, Yakutsk 677010, Russia.
Доп.точки доступа:
Prudnikova, Svetlana, V; Evgrafova, Svetlana Yu; Volova, Tatiana G.; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Кл.слова (ненормированные):
Siberian cryogenic soils -- P(3HB) bioplastic -- metabolic activity -- structure of microbial community -- P(3HB)-degrading strains -- P(3HB) -- properties
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, VN Sukachev Inst Forest, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Melnikov Permafrost Inst, 36 Merzlotnaya St, Yakutsk 677010, Russia.
Доп.точки доступа:
Prudnikova, Svetlana, V; Evgrafova, Svetlana Yu; Volova, Tatiana G.; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Biotechnological aspects of bioconversion of post-extraction biomass and cell culture Orthilia secunda (L.) house with basidiomycetes
/ Zh. A. Koh, Yu. A. Litovka, R. Kh. Enazarov [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 359-369, DOI 10.14258/JCPRM.2020048392
. - ISSN 1029-5151
Перевод заглавия: Биотехнологические аспекты утилизации послеэкстракционной биомассы и клеточной культуры orthilia secunda (L.) house базидиальными грибами
Кл.слова (ненормированные):
Basidiomycetes -- Biodegradation -- Callus tissue -- Orthilia secunda -- Pleurotus eryngii -- Post-extraction residue -- Solid-phase cultivation -- Trametes versicolor
Аннотация: The possibility of bioconversion after the extraction residue of the medicinal plant Orthilia secunda (initial plant biomass and callus after extraction of biologically active substances) by fast growing strains of basidiomycetes Tv2-16K Trametes versicolor and Pe-17T Pleurotus eryngii was studied. The main target products are: a mycelial-plant product with a reduced content of the indigestible fraction and enriched in protein and full-fledged fruit bodies. Fungi colonize plant waste with a radial growth rate of 2.0-2.3 mm / day and a growth rate of 65-77 units. Phenol oxidase activity is 0.7-1.2 units/g·s, depending on the type after the extraction residue. In substrates after biodegradation, the proportion of difficult hydrolysable polysaccharides, easily hydrolysable polysaccharides, and lignin is on average 1.6 times lower than in the original substrate. The content of protein and extractive substances is significantly higher, especially after the extraction residue of O. secunda callus under the influence of the T. versicolor strain Tv2-16K: 12.8 and 24.3%, respectively, of the mass of dry substrate. Fruit bodies of P. eryngii strain Pe-17T were obtained on various compositions of plant substrates. The maximum fruit formation was noted on four-component substrates containing two types after extraction residues of O. secunda, wheat bran, birch or aspen sawdust: the average weight of fruit bodies from one block was 230-236 g; biological efficiency - 46-47.2%. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Enazarov, R. Kh.; Makolova, P. V.; Shimova, Yu. S.; Pochekutov, I. S.; Pavlov, I. N.
Перевод заглавия: Биотехнологические аспекты утилизации послеэкстракционной биомассы и клеточной культуры orthilia secunda (L.) house базидиальными грибами
Кл.слова (ненормированные):
Basidiomycetes -- Biodegradation -- Callus tissue -- Orthilia secunda -- Pleurotus eryngii -- Post-extraction residue -- Solid-phase cultivation -- Trametes versicolor
Аннотация: The possibility of bioconversion after the extraction residue of the medicinal plant Orthilia secunda (initial plant biomass and callus after extraction of biologically active substances) by fast growing strains of basidiomycetes Tv2-16K Trametes versicolor and Pe-17T Pleurotus eryngii was studied. The main target products are: a mycelial-plant product with a reduced content of the indigestible fraction and enriched in protein and full-fledged fruit bodies. Fungi colonize plant waste with a radial growth rate of 2.0-2.3 mm / day and a growth rate of 65-77 units. Phenol oxidase activity is 0.7-1.2 units/g·s, depending on the type after the extraction residue. In substrates after biodegradation, the proportion of difficult hydrolysable polysaccharides, easily hydrolysable polysaccharides, and lignin is on average 1.6 times lower than in the original substrate. The content of protein and extractive substances is significantly higher, especially after the extraction residue of O. secunda callus under the influence of the T. versicolor strain Tv2-16K: 12.8 and 24.3%, respectively, of the mass of dry substrate. Fruit bodies of P. eryngii strain Pe-17T were obtained on various compositions of plant substrates. The maximum fruit formation was noted on four-component substrates containing two types after extraction residues of O. secunda, wheat bran, birch or aspen sawdust: the average weight of fruit bodies from one block was 230-236 g; biological efficiency - 46-47.2%. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Enazarov, R. Kh.; Makolova, P. V.; Shimova, Yu. S.; Pochekutov, I. S.; Pavlov, I. N.
Biotechnological potential of the Siberian strains of basidiomycetes - producers of lignolytic and cellulolytic enzymes
/ Yu. A. Litovka, I. N. Pavlov, P. V. Makolova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 371-383, DOI 10.14258/JCPRM.2020048396
. - ISSN 1029-5151
Перевод заглавия: Биотехнологический потенциал сибирских штаммов базидиальных грибов - продуцентов ферментов лигноцеллюлазного действия
Кл.слова (ненормированные):
Basidiomycetes -- Biodegradation -- Cellulases -- Hydrodynamic activation -- Ligninases -- Plant substrates -- Solid-phase and deep cultivation -- Xylotrophs
Аннотация: The results of a study of the wood-destroying properties of Siberian strains of xylotrophic basidiomycetes (Armillaria, Ganoderma, Fomitopsis, Heterobasidion and Porodaedalea) are presented. The growth parameters and enzymatic activity of the strains were determined during solid-phase and deep cultivation. Fomitopsis pinicola and Ganoderma lucidum are fast-growing fungi on cellulose-, tannin-containing nutrient media, coniferous and deciduous plant substrates (source and hydrodynamically activated). The growth coefficient on media with tannin and Na-carboxymethyl cellulose is more than 45; on plant substrates - more than 30. The strains exhibit a different growth reaction to the preliminary activation of birch sawdust (growth acceleration / growth slowdown / indifference). The maximum wood-destroying activity on wood of A. sibirica noted for the fungi A. borealis, G. tsugae, G. lucidum, F. pinicola, and P. niemelaei. The decrease in substrate mass was 8-11%; the amount of polysaccharides decreased on average 1.4 times mainly due to the fermentolysis of hard-hydrolyzable polysaccharides. High enzymatic activity of fungi observed during solid-phase and deep cultivation with an inducer. The maximum activity of phenol oxidase is characteristic of G. tsugae (1.21 units/g·s); carboxymethyl cellulase - for F.pinicola and G. lucidum strains (11.8 and 10.3 units/ml, respectively); xylanases - for H. abietinum (3.8 u/ml). The maximum accumulation of extracellular protein observed in F. pinicola (0.89 mg/ml). According to the totality of rapid test indicators, quantitative determination of enzymatic activity, growth parameters on lignocellulosic substrates and the degree of wood bioconversion, the most promising producer of lignolytic enzymes in vitro is the Gl4-16A Ganoderma lucidum strain; cellulolytic enzymes - strain Fp6-17 Fomitopsis pinicola. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Institute of Physics L.V. Kirensky, SB, RAS, Akademgorodok, 50/43, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Litovka, Yu. A.; Pavlov, I. N.; Makolova, P. V.; Timofeev, A. A.; Litvinova, E. A.; Vasil'Eva, A. A.; Shabanov, A. V.
Перевод заглавия: Биотехнологический потенциал сибирских штаммов базидиальных грибов - продуцентов ферментов лигноцеллюлазного действия
Кл.слова (ненормированные):
Basidiomycetes -- Biodegradation -- Cellulases -- Hydrodynamic activation -- Ligninases -- Plant substrates -- Solid-phase and deep cultivation -- Xylotrophs
Аннотация: The results of a study of the wood-destroying properties of Siberian strains of xylotrophic basidiomycetes (Armillaria, Ganoderma, Fomitopsis, Heterobasidion and Porodaedalea) are presented. The growth parameters and enzymatic activity of the strains were determined during solid-phase and deep cultivation. Fomitopsis pinicola and Ganoderma lucidum are fast-growing fungi on cellulose-, tannin-containing nutrient media, coniferous and deciduous plant substrates (source and hydrodynamically activated). The growth coefficient on media with tannin and Na-carboxymethyl cellulose is more than 45; on plant substrates - more than 30. The strains exhibit a different growth reaction to the preliminary activation of birch sawdust (growth acceleration / growth slowdown / indifference). The maximum wood-destroying activity on wood of A. sibirica noted for the fungi A. borealis, G. tsugae, G. lucidum, F. pinicola, and P. niemelaei. The decrease in substrate mass was 8-11%; the amount of polysaccharides decreased on average 1.4 times mainly due to the fermentolysis of hard-hydrolyzable polysaccharides. High enzymatic activity of fungi observed during solid-phase and deep cultivation with an inducer. The maximum activity of phenol oxidase is characteristic of G. tsugae (1.21 units/g·s); carboxymethyl cellulase - for F.pinicola and G. lucidum strains (11.8 and 10.3 units/ml, respectively); xylanases - for H. abietinum (3.8 u/ml). The maximum accumulation of extracellular protein observed in F. pinicola (0.89 mg/ml). According to the totality of rapid test indicators, quantitative determination of enzymatic activity, growth parameters on lignocellulosic substrates and the degree of wood bioconversion, the most promising producer of lignolytic enzymes in vitro is the Gl4-16A Ganoderma lucidum strain; cellulolytic enzymes - strain Fp6-17 Fomitopsis pinicola. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Institute of Physics L.V. Kirensky, SB, RAS, Akademgorodok, 50/43, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Litovka, Yu. A.; Pavlov, I. N.; Makolova, P. V.; Timofeev, A. A.; Litvinova, E. A.; Vasil'Eva, A. A.; Shabanov, A. V.
Component Composition of the Biodeg-Radation Product of Fallen Leaves by Basidiomycetes Pleurotus Pulmonarius (Strain Pp-3.2)
/ O. O. Mamaeva, E. V. Isaeva, S. R. Loskutov, M. A. Plyashechnik // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 1. - С. 277-285, DOI 10.14258/JCPRM.2021018851
. - ISSN 1029-5151
Кл.слова (ненормированные):
amino acid analysis -- Bioconversion -- chemical composition -- digestibility -- heavy metals -- litter -- nucleic acids -- Pleurotus pulmonarius. -- poplar -- protein feed product
Аннотация: Today, plant waste, including fallen poplar leaves (litter), are a promising raw material for the production of useful prod-ucts using bioconversion, such as protein feed additives. The aim of this study was to study the component composition of the products obtained as a result of the bioconversion of leaf litter. The strain PP-3.2 Pleurotus pulmonarius (Fr.) Quel. In the process of substrate conversion, strain PP-3.2 primarily utilizes extractives and easily hydrolyzable polysaccharides, the amount of which decreases by 44 and 36%, respectively. The total content of polysaccharides is reduced by 20%, lignin substances-9.4%. The loss of substrate mass based on leaf litter was 23%. The product obtained after cultivation contains 28% protein. Analysis of the amino acid composition of the protein showed a high rate of phenylalanine with tyrosine (115), threonine (117.5), valine (110) and isoleucine (105%). Also, the biodegradation product has a high content of glutamic and aspartic acids (11.2 and 25.3% of the total amino acids, respectively). The levels of cadmium, lead and copper revealed as a result of studies do not exceed the maximum permissible concentrations established by the veterinary department. At the same time, there is a high content of iron (166.0 Eg/kg) and zinc (256.7 mg/kg) in the feed product. The digestibility of the product is 54%, the content of nucleic substances is up to 0.3%. Thus, the results obtained show the possibility of using the product of the conversion of poplar fallen leaves as a protein feed additive. © 2021 Altai State University. All rights reserved.
Scopus
Держатели документа:
Reshetnev Siberian State University of Science and Technology, pr. Krasnoyarskii Rabochii, 31, Krasnoyarsk, 660037, Russian Federation
Sukachev Institute of Forest SB RAS, Federal Research Center «Krasnoyarsk Scientific Center SB RAS», Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Mamaeva, O. O.; Isaeva, E. V.; Loskutov, S. R.; Plyashechnik, M. A.
Кл.слова (ненормированные):
amino acid analysis -- Bioconversion -- chemical composition -- digestibility -- heavy metals -- litter -- nucleic acids -- Pleurotus pulmonarius. -- poplar -- protein feed product
Аннотация: Today, plant waste, including fallen poplar leaves (litter), are a promising raw material for the production of useful prod-ucts using bioconversion, such as protein feed additives. The aim of this study was to study the component composition of the products obtained as a result of the bioconversion of leaf litter. The strain PP-3.2 Pleurotus pulmonarius (Fr.) Quel. In the process of substrate conversion, strain PP-3.2 primarily utilizes extractives and easily hydrolyzable polysaccharides, the amount of which decreases by 44 and 36%, respectively. The total content of polysaccharides is reduced by 20%, lignin substances-9.4%. The loss of substrate mass based on leaf litter was 23%. The product obtained after cultivation contains 28% protein. Analysis of the amino acid composition of the protein showed a high rate of phenylalanine with tyrosine (115), threonine (117.5), valine (110) and isoleucine (105%). Also, the biodegradation product has a high content of glutamic and aspartic acids (11.2 and 25.3% of the total amino acids, respectively). The levels of cadmium, lead and copper revealed as a result of studies do not exceed the maximum permissible concentrations established by the veterinary department. At the same time, there is a high content of iron (166.0 Eg/kg) and zinc (256.7 mg/kg) in the feed product. The digestibility of the product is 54%, the content of nucleic substances is up to 0.3%. Thus, the results obtained show the possibility of using the product of the conversion of poplar fallen leaves as a protein feed additive. © 2021 Altai State University. All rights reserved.
Scopus
Держатели документа:
Reshetnev Siberian State University of Science and Technology, pr. Krasnoyarskii Rabochii, 31, Krasnoyarsk, 660037, Russian Federation
Sukachev Institute of Forest SB RAS, Federal Research Center «Krasnoyarsk Scientific Center SB RAS», Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Mamaeva, O. O.; Isaeva, E. V.; Loskutov, S. R.; Plyashechnik, M. A.
Novel materials for myco-decontamination of cyanide-containing wastewaters through microbial biotechnology
/ I. N. Pavlov, Y. A. Litovka // (1 March 2021 through 5 March 2021 : Trans Tech Publications Ltd, 2021. - Vol. 1037 MSF. - P751-758, DOI 10.4028/www.scientific.net/MSF.1037.751
. -
Кл.слова (ненормированные):
Biodegradation -- Biotechnology -- Cyanide wastewater -- Fusarium oxysporum -- Mycelium-based materials -- Biomass -- Bioremediation -- Biotechnology -- Fungi -- Tailings -- Cyanide wastewater -- Fusarium -- Fusarium oxysporums -- Lignocellulosic material -- Microbial biotechnology -- Mycelium-based material -- Novel materials -- Submerged condition -- Tailing dam -- Wood samples -- Biodegradation
Аннотация: This study examined the effectiveness of decontamination of industrial cyanide-containing water using mycelium-based lignocellulosic materials. These results suggest that fungi biomass and plant substrates can be used successfully in the treatment of wastewater contaminated by cyanide. Fungi were isolated from old wood samples taken from a tailing dam with high cyanide content (more than 20 years in semi-submerged condition). All isolated fungi belonged to the genus Fusarium. Fusarium oxysporum Schltdl is most effective for biodegradation of cyanide-containing wastewaters (even at low temperatures). The most optimal lignocellulosic composition for production of mycelium-based biomaterial for biodegradation of cyanide wastewater consists of a uniform ratio of Siberian pine sawdust and wheat straw. The high efficiency of mycelium-based materials has been experimentally proven in vitro at 15-25 ° C. New fungal biomaterials are provide decrease in the concentration of cyanide ions to 79% (P <0.001). Large-scale cultivation of fungi biomass was carried out by the periodic liquid-phase cultivation. The submerged biomass from bioreactor was used as an inoculum for the production of mycelium-based materials for bioremediation of cyanide wastewater in situ (gold mine tailing). © 2021 Trans Tech Publications Ltd, Switzerland.
Scopus
Держатели документа:
V.N. Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Department of Chemical Technology of Wood and Biotechnology, Reshetnev Siberian State University of Science and Technology, Krasnoyarsk, 660037, Russian Federation
Доп.точки доступа:
Pavlov, I. N.; Litovka, Y. A.
Кл.слова (ненормированные):
Biodegradation -- Biotechnology -- Cyanide wastewater -- Fusarium oxysporum -- Mycelium-based materials -- Biomass -- Bioremediation -- Biotechnology -- Fungi -- Tailings -- Cyanide wastewater -- Fusarium -- Fusarium oxysporums -- Lignocellulosic material -- Microbial biotechnology -- Mycelium-based material -- Novel materials -- Submerged condition -- Tailing dam -- Wood samples -- Biodegradation
Аннотация: This study examined the effectiveness of decontamination of industrial cyanide-containing water using mycelium-based lignocellulosic materials. These results suggest that fungi biomass and plant substrates can be used successfully in the treatment of wastewater contaminated by cyanide. Fungi were isolated from old wood samples taken from a tailing dam with high cyanide content (more than 20 years in semi-submerged condition). All isolated fungi belonged to the genus Fusarium. Fusarium oxysporum Schltdl is most effective for biodegradation of cyanide-containing wastewaters (even at low temperatures). The most optimal lignocellulosic composition for production of mycelium-based biomaterial for biodegradation of cyanide wastewater consists of a uniform ratio of Siberian pine sawdust and wheat straw. The high efficiency of mycelium-based materials has been experimentally proven in vitro at 15-25 ° C. New fungal biomaterials are provide decrease in the concentration of cyanide ions to 79% (P <0.001). Large-scale cultivation of fungi biomass was carried out by the periodic liquid-phase cultivation. The submerged biomass from bioreactor was used as an inoculum for the production of mycelium-based materials for bioremediation of cyanide wastewater in situ (gold mine tailing). © 2021 Trans Tech Publications Ltd, Switzerland.
Scopus
Держатели документа:
V.N. Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Department of Chemical Technology of Wood and Biotechnology, Reshetnev Siberian State University of Science and Technology, Krasnoyarsk, 660037, Russian Federation
Доп.точки доступа:
Pavlov, I. N.; Litovka, Y. A.
N Synthesis and Properties of Polymers Based on Styrene and alpha-angelicalactone
/ K. L. Kaigorodov, V. E. Tarabanko, S. R. Loskutov [et al.] // J. Sib. Fed. Univ.-Chem. - 2022. - Vol. 15, Is. 1. - P5-13, DOI 10.17516/1998-2836-0266. - Cited References:17
. - ISSN 1998-2836. - ISSN 2313-6049
РУБ Chemistry, Multidisciplinary
Аннотация: Polyangelicalactone-graft-polystyrene copolymers were obtained through cationic polymerization. Increasing the portion of styrene units in the copolymers improves the strength properties and increases fragility. Increasing the polyangelicalactone(PAL) content in the copolymers increases the elasticity of the materials obtained. The obtained copolymers are greatly a composition of PAL and polystyrene (PS)with a small content of bonds of styrene-units of 4-alkoxypent-3-enoic acid.Low alpha-angelicalactone(AL) content in styrene copolymers improves the oxidative stability of the copolymers. The resulting PAL-graft-PScopolymers have physical and mechanical properties corresponding to the requirements for general-purpose PS.Y
WOS
Держатели документа:
FRC Krasnoyarsk Sci Ctr SB RAS, Inst Chem & Chem Technol SB RAS, Krasnoyarsk, Russia.
Sukachev Inst Forest SB RAS, Krasnoyarsk, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Доп.точки доступа:
Kaigorodov, Konstantin L.; Tarabanko, Valery E.; Loskutov, Sergei R.; Mazurova, Elena, V; Kondrasenko, Alexander A.; Voronchikhin, Vasiliy D.; Smirnova, Marina A.; Malyar, Yuri N.; Vigul, Dmitry O.
Рубрики:
COPOLYMERIZATION
BIODEGRADATION
POLYSTYRENE
Кл.слова (ненормированные):
copolymerization -- polystyrene -- a--angelicalactone -- polyangelicalactone -- poly[oxidiyl(4-methyl-1-oxobut-3-en-1,4-diyl)] -- poly[oxidiyl(4-methyl-1-oxobut-3-en-1,4-diyl)]-graft-poly(1-phenylethyle -- ne)
COPOLYMERIZATION
BIODEGRADATION
POLYSTYRENE
Кл.слова (ненормированные):
copolymerization -- polystyrene -- a--angelicalactone -- polyangelicalactone -- poly[oxidiyl(4-methyl-1-oxobut-3-en-1,4-diyl)] -- poly[oxidiyl(4-methyl-1-oxobut-3-en-1,4-diyl)]-graft-poly(1-phenylethyle -- ne)
Аннотация: Polyangelicalactone-graft-polystyrene copolymers were obtained through cationic polymerization. Increasing the portion of styrene units in the copolymers improves the strength properties and increases fragility. Increasing the polyangelicalactone(PAL) content in the copolymers increases the elasticity of the materials obtained. The obtained copolymers are greatly a composition of PAL and polystyrene (PS)with a small content of bonds of styrene-units of 4-alkoxypent-3-enoic acid.Low alpha-angelicalactone(AL) content in styrene copolymers improves the oxidative stability of the copolymers. The resulting PAL-graft-PScopolymers have physical and mechanical properties corresponding to the requirements for general-purpose PS.Y
WOS
Держатели документа:
FRC Krasnoyarsk Sci Ctr SB RAS, Inst Chem & Chem Technol SB RAS, Krasnoyarsk, Russia.
Sukachev Inst Forest SB RAS, Krasnoyarsk, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Доп.точки доступа:
Kaigorodov, Konstantin L.; Tarabanko, Valery E.; Loskutov, Sergei R.; Mazurova, Elena, V; Kondrasenko, Alexander A.; Voronchikhin, Vasiliy D.; Smirnova, Marina A.; Malyar, Yuri N.; Vigul, Dmitry O.