Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

: материалы временных коллективов / G. V. Kuznetsova // Eurasian Journal of Forest Research. - 2008. - Vol. 11-2. - С. 81-87. - Библиогр. в конце ст.
Аннотация: Experiments in intraspecific hybridization of Siberian stone pine, not just of this species but of different regional origins as well, have been carried out at a clone plantation in Middle Siberia (Krasnoyars forest-steppe). Crossings were realized using the principle of ecologo-geographical remoteness of populations. Siberian stone pine clones chosen for crossing had good growth and constant reproductive ability over many years. An analysis of the characteristics of hybrid female cones (weight, linear size, number of developed scales) and seeds (number, weight, seed fullness, viability) showed the positive influence of controlled pollination with combinations of different climatypes. The study of hybrid growing climatypes, resulting from crossing of the plain and mountain populations, also revealed signs of heterosis.

Держатели документа:
Институт леса им. В.Н. Сукачева Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок, 50, стр., 28

Доп.точки доступа:
Кузнецова, Галина Васильевна

/ I. V. Tikhonova, V. V. Tarakanov, A. A. Knorre // Russ. J. Ecol. - 2012. - Vol. 43, Is. 3. - P179-184, DOI 10.1134/S1067413612030150. - Cited References: 23. - This study was supported by the Russian Foundation for Basic Research (project no. 11-04-98008). . - 6. - ISSN 1067-4136РУБ Ecology

Аннотация: Variation in radial increments of 28-year-old L. clones in response to changes in weather conditions during the period between 1986 and 2007 has been studied in the Sredneobskii Bor pine forest. Significant genotypic differences in the adaptive reaction norm of the clones have been revealed. It has been shown that the contribution of genotypic differences to the total variance of test characters is usually significant and depends both on the conditions of growing season and on a given increment character.

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Держатели документа:
[Tikhonova, I. V.
Tarakanov, V. V.
Knorre, A. A.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Tikhonova, I.V.; Tarakanov, V.V.; Knorre, A.A.

[Текст] / D. L. Grodnitsky // Zhurnal Obshchei Biol. - 1999. - Vol. 60, Is. 5. - С. 488-509. - Cited References: 158 . - 22. - ISSN 0044-4596РУБ Biology

Аннотация: Consequences derived from the synthetic theory of evolution are in contradiction with facts. The theory affirms that the evolution rate depends on gene variability, intensity of recombination and press of natural selection. It means that fecund organisms with short life cycle have to evolve very quickly. In reality the quickest evolution is observed in mammals, for example in elephants. The theory predicts that evolution can not base only on mutation in the absence of recombination. But parthenogenetic and asexual species evolve very quick, and their variability is not less than that of sexual forms. Contrarily to the neo-Darwinism ideology, evolution takes place not only in constant environment and is often absent even in changing ecosystems. Reproductive isolation and fluctuations in population number are not sufficient conditions for the beginning of evolutionary changes. Numerous data show that the space of opportunities where evolving organisms can travel is constrained by morpho-functional and morpho-genetic factors more than it was considered by the synthetic theory of evolution. It means that evolution should be explained not only in terms of genome, but also using onthogenetic development of an organism and its interactions with environment. Application of organism-focused terminology is possible in the frame of the theory also based on the idea of natural selection, but providing different prognoses, sometimes opposite to the neo-Darwinistic ones.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest Res, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Grodnitsky, D.L.

[Text] / K. P. Kutsenogyi [et al.] // Nucl. Instrum. Methods Phys. Res. Sect. A-Accel. Spectrom. Dect. Assoc. Equip. - 2007. - Vol. 575: 16th International Synchrotron Radiation Conference (SR-2006) (JUL 10-14, 2006, Novosibirsk, RUSSIA), Is. 01.02.2013. - P214-217, DOI 10.1016/j.nima.2007.01.071. - Cited References: 9 . - 4. - ISSN 0168-9002РУБ Instruments & Instrumentation + Nuclear Science & Technology + Physics, Particles & Fields + Spectroscopy

Аннотация: The method of X-ray fluorescence analysis with synchrotron radiation was used to estimate soil and phytomass elemental composition in the long-term field experiments with clones and climatypes of Scots pine Pinus sylvestris L. in West Siberia. Overall from 15 to 21 chemical elements (Fe, Ca, K, Ti, Mn, Zr, Sr, Rb, Co, Cr, Zn, V, Y, Sc, Nb, Pb, Ga, Cu, Ni, As, Mo) were detected. The elemental composition of dried needles and needle ash were compared. The influence of genetic variability of Scots pine on some element concentration in the topsoil was revealed. The temporal dynamics of interclonal variability of elemental composition of pine needles was revealed. (c) 2007 Elsevier B.V. All rights reserved.

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Держатели документа:
Inst Forest, W Siberian Off, Novosibirsk 630082, Russia
SB RAS, Inst Chem Kinet & Combust, Novosibirsk 630090, Russia
SB RAS, Inst Soil Sci & Agrochem, Novosibirsk 630090, Russia
SB RAS, Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Kutsenogyi, K.P.; Makarikova, R.P.; Milyutin, L.I.; Naumova, N.B.; Tarakanov, V.V.; Chankina, O.V.

/ G. V. Kuznetsova // Ontogenez. - 2004. - Vol. 35, Is. 2. - С. 62-68 . - ISSN 0475-1450
Аннотация: The development of male generative organs of the Siberian stone pine clones of different origin was analyzed in the Krasnoyarsk forest-steppe for many years. Geographical and individual variability was described as concerns the number of microstrobils and pollen variability. The beginning of "flowering", abundance of microstrobils, and their regular formation were shown to markedly vary and, all other conditions being equal, be determined by the presence of clones, their hereditary features, and environmental conditions, as well by the influence of stock.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia.

Доп.точки доступа:
Kuznetsova, G.V.

[Text] / I. N. Tret'yakova, E. V. Voroshilova, D. N. Shuvaev // Russ. J. Plant Physiol. - 2014. - Vol. 61, Is. 2. - P274-280, DOI 10.1134/S1021443714020162. - Cited References: 24. - This work was supported by the Russian Foundation for Basic Research, project no. 11-04-00281-a; and a r-Sibir'-a research grant, project no. 13-04-98045. . - ISSN 1021-4437. - ISSN 1608-3407РУБ Plant Sciences

Аннотация: The results of long-term work on the induction of somatic embryogenesis in Siberian pine (Pinus sibirica Du Tour) growing in a natural stand of trees and in clone grafting plantation located in the Western Sayan are shown. Controlled pollination of the clones of Siberian pine had a positive influence on the state of callus cultures. The cytological analysis of embryonal-suspensor mass made it possible to identify embryological structures morphologically close to zygotic embryos at early developmental stages; as a result, the callus tissue was recognized embryogenic. We revealed donor plants (clones), whose zygotic embryos in vitro can serve as a source of embryogenic callus tissue.

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Держатели документа:
[Tret'yakova, I. N.
Voroshilova, E. V.
Shuvaev, D. N.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia
ИЛ СО РАН

Доп.точки доступа:
Tret'yakova, I.N.; Voroshilova, E.V.; Shuvaev, D.N.; Russian Foundation for Basic Research [11-04-00281-a]; a r-Sibir'-a research grant [13-04-98045]

/ I. V. Tikhonova [et al.] // Russ. J. Ecol. - 2015. - Vol. 46, Is. 6. - P503-510, DOI 10.1134/S1067413615060193 . - ISSN 1067-4136
Аннотация: Individual correlations between radial increments of trees and meteorological environmental factors were investigated in 28-year-old clone cultures of Scotch pine (Pinus sylvestris L.) growing at an experimental site of Forestry Institute, Siberian Branch, Russian Academy of Sciences, in the Sredneobskiy pine forest. Significant genotypic differences were revealed between the clones in the correlation coefficients of the indices of annual increments and the indices of increments of early and late wood with indicators of heat and moisture availability for trees in 1986–2007. Autocorrelation established a significant genetic differentiation of clones from one to three orders of magnitude. © 2015, Pleiades Publishing, Ltd.

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Текст статьи,
Полный текст Springer

Держатели документа:
Sukachev Forest Institute, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Tikhonova, I. V.; Tarakanov, V. V.; Knorre, A. A.; Tikhonova, N. A.

[Текст] : научное издание / В.В. Тараканов [и др.] // Сибирский лесной журнал. - 2017. - № : 1. - С. 95-104 : табл., DOI 10.15372/SJFS20170110. - Библиогр. в конце ст. . - ISSN 2311-1410
   Перевод заглавия: VARIABILITY OF NEEDLE ANTIMICROBIC ACTIVITY IN CLONE PLANTATION OF SCOTS PINE <i>Pinus sylvestris</i> L

УДК

577.19

582.475+581.522.4 (571.1)

Аннотация: Оценена антимикробная активность летучих соединений хвои 23-летних клонов <i>Pinus sylvestris</i> L. методом «опарения» штриховых посевов микробных культур в чашках Петри. Исследования проведены 18 и 26 июля 2011 г. на трех тест-объектах - грамположительных бактериях <i>Staphylococcus epidermidis</i>, грамотрицательных бактериях <i>Esсherichia coli</i> и дрожжеподобных грибах <i>Candida albicans</i>. Оценка антимикробного действия сосны проводилась в сравнении с контролем по шкале фитонцидной активности в баллах от 0 (отсутствие эффекта) до 4 (сильный эффект). Средний балл фитонцидной активности клонов по отношению к отдельным тест-системам варьировал в пределах 0.00-3.00. Выявлены слабая дифференциация клонов сосны к <i>Staphylococcus epidermidis</i> и отсутствие достоверных межклоновых различий по воздействию эфирных масел сосны на остальные микробные тест-объекты. Частота парных сравнений, в которых обнаружены достоверные различия между клонами, составляет менее 5 % для S. epidermidis и около 1.5 % для всех тест-микробов. Наряду с этим прослеживались высокодостоверные эффекты времени проведения тестов (календарной даты), вида тест-объекта («патогена») и взаимодействия «дата тестирования - патогены» и «дата тестирования - клоны», на которые в 3-факторном дисперсионном анализе приходится около 23, 33 и 15 % соответственно. Наиболее вероятными причинами этого являются динамика состава летучих соединений хвои и взаимодействие генотип - среда, что должно стать объектом более пристальных исследований. Очень важным в этой связи является вопрос о сезоне, который оптимален для надежного выявления генотипов сосны, отличающихся максимальной фитонцидностью. Выявлена отрицательная межклоновая (генотипическая) корреляция между фитонцидностью сосны к <i>S. epidermidis</i> и к <i>C. albicans</i>. Возможно, это обусловлено обратно пропорциональной зависимостью между содержанием определенных компонентов в эфирных маслах, которые отвечают за ингибирование различных патогенных организмов. При анализе генетических особенностей протестированных клонов выделяются их совокупности, достоверно различающиеся, с одной стороны, по фитонцидности к стафилококку <i>S. epidermidis</i>, с другой - по индивидуальной гетерозиготности аллозимных локусов. Такого рода дифференциация генотипов отмечена впервые и также может быть в фокусе дальнейших исследований.
Streak inoculation of microbial test-cultures was treated with volatile emissions of needles of 23-years clones of <i>Pinus sylvestris</i> L. The study was conducted in July 2011 on three test-objects - gram-positive bacteria <i>Staphyllococcusepidermidis</i>, gram-negative bacteria <i>Esсherichia coli</i> and yeast-like fungi <i>Candida albicans</i>. Antimicrobial effect of pine volatile emissions was assessed in comparison with the control on 0 (absence of the effect) - 4 (strong effect) scale of antimicrobial activity. The mean marks of antimicrobial activity of clones, calculated in relation to separate test-systems, were within the limits of 0.00-3.00. The weak differentiation of pine clones to <i>S. epidermidis</i> is revealed. Frequency of pair comparisons in which statistically significant differences between clones was found to be less than 5 % for <i>S. epidermidis</i> and about 1.5 % for all the test-microbes. Alongside with it significant effects of the time of testing (calendar dates), a microbial species («pathogens») and interactions «date of testing-pathogens» and «date of testing-clones» were revealed: in ANOVA their effects amounted to nearly 23, 33 and 15 % of the total dispersion accordingly. The most probable reasons for it are dynamics of structure of essential oils and «genotype-environment» interaction that should become object of future research. In this connection, an important question is what is the season that is optimum for reliable revealing of the pine genotypes with maximal antimicrobial activity? Negative between-clones (between-genotypes) correlations between antimicrobial activity to <i>S. epidermidis</i> and to <i>C. albicans</i> are revealed. It is probably caused by inversely proportional dependence between concentrations of the components in essential oils, which are responsible for inhibition of various pathogenic organisms. The sample of investigated clones was divided into two sub-samples that sufficiently differed by the levels of both antimicrobial activity to <i>S. epidermidis</i> and individual heterozygosity of allozyme's loci. Such differentiation of genotypes is noted for the first time, and can be in focus of further studies.

РИНЦ

Держатели документа:
Институт леса им. В. Н. Сукачева СО РАН
Новосибирский государственный аграрный университет
Центральный сибирский ботанический сад СО РАН

Доп.точки доступа:
Тараканов, Вячеслав Вениаминович; Tarakanov V.V.; Чиндяева, Людмила Николаевна; Chindyaeva L.N.; Цыбуля, Наталья Владимировна; Tsybulya N.V.; Тихонова, Ирина Васильевна; Tikhonova Irina Vasil'yevna

/ R. Linnakoski, S. Sutela, MPA Coetzee [et al.] // Sci Rep. - 2021. - Vol. 11, Is. 1. - Ст. 7336, DOI 10.1038/s41598-021-86343-7. - Cited References:88. - Tuija Hytonen, Alex Nordlund and Runlei Chang are thanked for excellent technical assistance and Pyry Veteli for providing Finnish isolates of Armillaria. The CSC - IT Center for Science, Finland, is acknowledged for providing computational resources. This work was funded by the Academy of Finland (decision number 309896). We are grateful to the 1KFG project (CSP 1974) for access to unpublished genome data. The genome sequence data were produced by the US Department of Energy Joint Genome Institute in collaboration with the user community, and we acknowledge the JGI team and the people who generated the material and RNA for the project: Francis M. Martin, Laszlo Nagy, Neha Sahu, Sara Hortal Botifoll, Johanna Wong-Bajracharya and Jonathan M. Plett. . - ISSN 2045-2322РУБ Multidisciplinary Sciences

Аннотация: Species of Armillaria are distributed globally and include some of the most important pathogens of forest and ornamental trees. Some of them form large long-living clones that are considered as one of the largest organisms on earth and are capable of long-range spore-mediated transfer as well as vegetative spread by drought-resistant hyphal cords called rhizomorphs. However, the virus community infecting these species has remained unknown. In this study we used dsRNA screening and high-throughput sequencing to search for possible virus infections in a collection of Armillaria isolates representing three different species: Armillaria mellea from South Africa, A. borealis from Finland and Russia (Siberia) and A. cepistipes from Finland. Our analysis revealed the presence of both negative-sense RNA viruses and positive-sense RNA viruses, while no dsRNA viruses were detected. The viruses included putative new members of virus families Mymonaviridae, Botourmiaviridae and Virgaviridae and members of a recently discovered virus group tentatively named "ambiviruses" with ambisense bicistronic genomic organization. We demonstrated that Armillaria isolates can be cured of viruses by thermal treatment, which enables the examination of virus effects on host growth and phenotype using isogenic virus-infected and virus-free strains.

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Держатели документа:
Nat Resources Inst Finland Luke, Helsinki, Finland.
Univ Pretoria, Forestry & Agr Biotechnol Inst FABI, Dept Biochem Genet & Microbiol, Pretoria, South Africa.
VN Sukachev Inst Forest SB RAS, Lab Reforestat Mycol & Plant Pathol, Krasnoyarsk, Russia.
Reshetnev Siberian State Univ Sci & Technol, Dept Chem Technol Wood & Biotechnol, Krasnoyarsk, Russia.

Доп.точки доступа:
Linnakoski, Riikka; Sutela, Suvi; Coetzee, Martin P. A.; Duong, Tuan A.; Pavlov, Igor N.; Litovka, Yulia A.; Hantula, Jarkko; Wingfield, Brenda D.; Vainio, Eeva J.; Litovka, Yulia; Academy of FinlandAcademy of FinlandEuropean Commission [309896]; 1KFG project [CSP 1974]

/ R. Linnakoski, S. Sutela, M. P.A. Coetzee [et al.] // Ботанический журнал. - 2021. - Т. 106, № 4. - P353-362, DOI 10.31857/S0006813621040116 . - ISSN 0006-8136
Аннотация: Species of Armillaria are distributed globally and include some of the most important pathogens of forest and ornamental trees. Some of them form large long-living clones that are considered as one of the largest organisms on earth and are capable of long-range spore-mediated transfer as well as vegetative spread by drought-resistant hyphal cords called rhizomorphs. However, the virus community infecting these species has remained unknown. In this study we used dsRNA screening and high-throughput sequencing to search for possible virus infections in a collection of Armillaria isolates representing three different species: Armillaria mellea from South Africa, A. borealis from Finland and Russia (Siberia) and A. cepistipes from Finland. Our analysis revealed the presence of both negative-sense RNA viruses and positive-sense RNA viruses, while no dsRNA viruses were detected. The viruses included putative new members of virus families Mymonaviridae, Botourmiaviridae and Virgaviridae and members of a recently discovered virus group tentatively named “ambiviruses” with ambisense bicistronic genomic organization. We demonstrated that Armillaria isolates can be cured of viruses by thermal treatment, which enables the examination of virus effects on host growth and phenotype using isogenic virus-infected and virus-free strains. © 2021, The Author(s).

Scopus

Держатели документа:
Natural Resources Institute Finland (Luke), Helsinki, Finland
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa
Laboratory of Reforestation, Mycology and Plant Pathology, V.N. Sukachev Institute of Forest SB RAS, Krasnoyarsk, Russian Federation
Department of Chemical Technology of Wood and Biotechnology, Reshetnev Siberian State University of Science and Technology, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Linnakoski, R.; Sutela, S.; Coetzee, M. P.A.; Duong, T. A.; Pavlov, I. N.; Litovka, Y. A.; Hantula, J.; Wingfield, B. D.; Vainio, E. J.

[Текст] : научное издание / М. Э. Пак, О. В. Горячкина, И. Н. Третьякова, Е. Н. Муратова // Сибирский экологический журнал. - 2023. - Т. 30, № 5. - С. 715-723, DOI 10.15372/SEJ20230512 . - ISSN 0869-8619
   Перевод заглавия: CYTOGENETIC CHARACTERISTICS OF EMBRYOGENIC CELL LINES OF DIFFERENT AGES OBTAINED VIA SOMATIC EMBRYOGENESIS IN LARIX SIBIRICA LEDEB

ГРНТИ

68.47.03

Аннотация: Представлены результаты цитогенетического исследования четырех эмбриогенных клеточных линий (КЛ) лиственницы сибирской (Larix sibirica Ledeb.) разной продолжительности культивирования: молодые (две четырехмесячные и 16-месячная КЛ) и длительно-пролиферирующая (11-летняя КЛ). Три клеточные линии являются диплоидными и содержат в кариотипе нормальное для данного вида число хромосом (2n = 24). Клеточная линия, которая культивировалась 16 месяцев, способная формировать соматические зародыши, оказалась анеуплоидной (2n = 25). Для данной клеточной линии, вероятно, характерна трисомия по одной из пар субметацентрических хромосом. Длительно пролиферирующая КЛ, от которой получены жизнеспособные плодоносящие клоны, в течение 11 лет культивирования сохраняет диплоидное число хромосом и является генетически стабильной. Кариотип хвойных растений характеризуется высокой степенью стабильности и большая часть видов семейства Pinaceae, к которому относится род Larix Mill., содержит 24 хромосомы (2n = 24). В связи с этим обнаружение цитогенетически устойчивых клеточных линий с измененным хромосомным набором представляет большой интерес для генетики этой группы растений. Выявление стабильно диплоидных клеточных линий актуально для генетико-селекционных работ, направленных на создание высокопродуктивных плантаций с заданными наследственными свойствами.
The results of cytogenetic analysis of four embryogenic cell lines of Siberian larch (Larix sibirica Ledeb.) of different ages: young (two four-month and 16-month CLs) and long-proliferating (11-year CL) are presented. Three cell lines are diploid and contain in the karyotype the normal number of chromosomes for this species (2n = 24). A 16-month-old CL capable of forming somatic embryos was aneuploid (2n = 25). This cell line is probably characterized by trisomy on one of the pairs of submetacentric chromosomes. The long-term proliferating CL, from which regenerants and clones were obtained, retains a diploid number of chromosomes during 11 years of cultivation and remains genetically stable. The karyotype of conifers is characterized by high degree of stability and most of the species contain 24 chromosomes (2n = 24). Therefore, the detection of cytogenetically stable cell lines is of great interest for genetic work of this group of plants and creation of highly productive plantations with specified hereditary properties.

РИНЦ

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Горячкина, Ольга Викторовна; Третьякова, Ираида Николаевна; Tret'yakova I.N.; Муратова, Елена Николаевна; Muratova, Elena Nikolayevna; Pak, M.

/ И. Н. Третьякова, М. Э. Пак // Лесоведение. - 2023. - № 5. - С. 526-536DOI 10.31857/S002411482305011X
   Перевод заглавия: SIBERIAN LARCH REPRODUCTION USING THE SOMATIC EMBRYOGENESIS BIOTECHNOLOGY
Аннотация: Биотехнология соматического эмбриогенеза в культуре in vitro в сочетании с геномной селекцией и криоконсервацией применяется для создания сортовых генетически тестированных быстрорастущих плантаций (программа Multi-Varietal Forestry MVF, Park, 2014, 2016, 2018). В институте леса им. Сукачева СО РАН в 2008 г. впервые была разработана биотехнология соматического эмбриогенеза для лиственницы сибирской (Larix sibirica Ledeb.) и получены 42 пролиферирующие клеточные линии, состоящие из эмбрионально-суспензорной массы (ЭСМ). Возраст клеточных линий достигает 13 лет. Между клеточными линиями наблюдалась значительная изменчивость по числу и размеру глобулярных зародышей в пролиферирующих эмбриогенных культурах, способности соматических зародышей созревать и прорастать. У разных клеточных линий на 1 г ЭСМ число глобулярных соматических зародышей колеблется от 2040 до 11103, созревает от 10 до 1220 зародышей. Регенеранты прорастают в ростовой камере, и клоны отдельных клеточных линий успешно растут в теплице и далее в почве лесопитомника на стационаре “Погорельский бор” ИЛ СО РАН. Генотипирование клонов по микросателлитным локусам показало полную их генетическую идентичность клеточной линии, из которой они были получены. У клонированных деревьев лиственницы сибирской в семилетнем возрасте произошла закладка генеративных органов. Таким образом, в настоящее время возможно оперативное внедрение программы MVF для плантационного лесовыращивания в России
The biotechnology of somatic embryogenesis in vitro, combined with genomic selection and cryopreservation is used to create varietal genetically tested fast-growing plantations (Multi-Varietal Forestry program (MVF), Park, 2014, 2016, 2018). In 2008, the Sukachev Forest Institute of the Siberian Branch of the RAS has developed for the first time the biotechnology of somatic embryogenesis for Siberian larch (Larix sibirica Ledeb.) and obtained 42 proliferating cell lines consisting of embryonal-suspensor mass (ESM). The age of cell lines reaches 13 years. Significant variability was observed between cell lines in the number and size of globular embryos in proliferating embryogenic cultures, and in the ability of somatic embryos to mature and germinate. In different cell lines, the number of globular somatic embryos per 1 g of ESMs fresh weight ranges from 2040 to 11103, with 10 to 1220 embryos maturing. The regenerants germinate in a growth chamber, and plantlets of individual cell lines grow successfully in a greenhouse and then in the soil of the forest nursery at the Forest Institute's Pogorelsky Bor station. Genotyping of clones at microsatellite loci showed their complete genetic identity to the cell line from which they were obtained. In cloned Siberian larch trees at the age of seven, the initiation of generative organs forming occurred. Thus, at present, it is possible to quickly implement the MVF program for plantation forestry in Russia.

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ИЛ СО РАН : 660036, Красноярск, Академгородок, 50/28

Доп.точки доступа:
Пак, Мария Эдуардовна; Pak, M.; Tret'yakova, Iraida Nikolayevna