: материалы временных коллективов / I. Tretyakova, A. Vyazovetskova, A. I. Ivanova // Eurasian journal of forest research. - 2006. - Vol. 9-1. - С. 37-44
Аннотация: The male buds of Siberian larch do not have an organic dormancy in the fall-winter period and, under favorable conditions, they are able to complete the development of the male generative structures. Cultivation of microsporophylls on MS medium containing 0.2-0.5 mg/1 of hormone 2,4-D, during a month, resulted in the unduction of androgenesis in vitro of Siberian larch. The increase of hormone concentration caused the development of necrotic processes. Data on androgenesis in vitro of Siberian larch, from representative gymnosperms, was obtained for the first time. Two development type embryos were obtained by cultivating microsporophylls of Siberian larch on MS medium.
Scopus
Держатели документа:
Институт леса им. В.Н. Сукачева Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок 50/28
Доп.точки доступа:
Vyazovetskova, Alyona S.; Вязовецкова А.С.; Ivanova, Anna Ivanovna; Иванова Анна Ивановна; Третьякова, Ираида Николаевна
Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН
w10=
Найдено документов в текущей БД: 22
Agrochemical and microbiological properties of technogenic soils on dumps in the Kansk-Rybinsk Depression
[Text] / I. D. Grodnitskaya, O. V. Trefilova, A. S. Shishikin // Eurasian Soil Sci. - 2010. - Vol. 43, Is. 7. - P810-821, DOI 10.1134/S1064229310070124. - Cited References: 39. - This work was supported in part by Program no. 23 of Basic Research of the Presidium of the Russian Academy of Sciences (project 1.3) and Research Project nos. 36A and 276.
. - 12. - ISSN 1064-2293
РУБ Soil Science
Аннотация: The agrochemical properties and microbiological activity of the soils on dump rocks of different ages and degrees of cultivation in the area of the Borodinsk coal strip mine were studied. The technogenic soils of the dumps significantly differed from the native soils in their total nitrogen content. In the young soils (less than 5 years old), the total nitrogen was present in trace amounts; in the 20-year-old dumps, its content was lower than in the control soils by 2.5 times. It was found that a humus horizon 2-3 cm thick was formed on the surface of the dumps after 20 years regardless of the reclamation practice and that the intensity of the biological processes was comparable to that in the control soils, because the microbial complexes functioned under conditions typical for automorphic soils. It was shown that the ecophysiological status of the 3- to 10-year-old soils was unstable and their microbocenoses were at the development stage.
Полный текст,
WOS,
Scopus
Держатели документа:
[Grodnitskaya, I. D.
Trefilova, O. V.
Shishikin, A. S.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Grodnitskaya, I.D.; Trefilova, O.V.; Shishikin, A.S.
Аннотация: The agrochemical properties and microbiological activity of the soils on dump rocks of different ages and degrees of cultivation in the area of the Borodinsk coal strip mine were studied. The technogenic soils of the dumps significantly differed from the native soils in their total nitrogen content. In the young soils (less than 5 years old), the total nitrogen was present in trace amounts; in the 20-year-old dumps, its content was lower than in the control soils by 2.5 times. It was found that a humus horizon 2-3 cm thick was formed on the surface of the dumps after 20 years regardless of the reclamation practice and that the intensity of the biological processes was comparable to that in the control soils, because the microbial complexes functioned under conditions typical for automorphic soils. It was shown that the ecophysiological status of the 3- to 10-year-old soils was unstable and their microbocenoses were at the development stage.
Полный текст,
WOS,
Scopus
Держатели документа:
[Grodnitskaya, I. D.
Trefilova, O. V.
Shishikin, A. S.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Grodnitskaya, I.D.; Trefilova, O.V.; Shishikin, A.S.
Specific features of the development of Siberian stone pine megagametophytes and embryos in vitro
/ I. N. Tret'iakova, N. V. Novoselova // Ontogenez. - 2003. - Vol. 34, Is. 4. - С. 282-291
. - ISSN 0475-1450
Кл.слова (ненормированные):
adenine -- drug derivative -- kinetin -- N benzyladenine -- N-benzyladenine -- naphthol derivative -- naphthyl acetate -- article -- chemistry -- culture medium -- culture technique -- cytology -- drug effect -- fertilization -- germination -- growth, development and aging -- methodology -- physiology -- pine -- plant -- plant seed -- Russian Federation -- zygote -- Adenine -- Cell Culture Techniques -- Culture Media -- Fertilization -- Germination -- Kinetin -- Naphthols -- Pinus -- Plant Shoots -- Seeds -- Siberia -- Zygote
Аннотация: Seedlings were grown in vitro from fertilized eggs and immature embryos of the Siberian stone pine. Cultivation of megagametophytes on a hormone-containing Murashige-Skoog medium from the egg formation until the globular embryo stage made it possible to manipulate fertilization and embryogenesis. Immature embryos are the most promising for in vitro cultivation. Their maturation and germination proceed within seven days of cultivation. When zygotic embryos were cultivated, adventitious buds were formed from cells at the cotyledon base and tips. When adventitious buds were subcultivated on a medium containing benzylaminopurine and naphthylacetic acid, organogenic callus and shoots were formed. Thus, cultivation of megagametophytes and embryos of the Siberian stone pine led to the completion of embryogenesis and formation of viable of seedlings.
Scopus,
Полный текст
Держатели документа:
Sukachev Institute of Forestry, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia.
Доп.точки доступа:
Tret'iakova, I.N.; Novoselova, N.V.
Кл.слова (ненормированные):
adenine -- drug derivative -- kinetin -- N benzyladenine -- N-benzyladenine -- naphthol derivative -- naphthyl acetate -- article -- chemistry -- culture medium -- culture technique -- cytology -- drug effect -- fertilization -- germination -- growth, development and aging -- methodology -- physiology -- pine -- plant -- plant seed -- Russian Federation -- zygote -- Adenine -- Cell Culture Techniques -- Culture Media -- Fertilization -- Germination -- Kinetin -- Naphthols -- Pinus -- Plant Shoots -- Seeds -- Siberia -- Zygote
Аннотация: Seedlings were grown in vitro from fertilized eggs and immature embryos of the Siberian stone pine. Cultivation of megagametophytes on a hormone-containing Murashige-Skoog medium from the egg formation until the globular embryo stage made it possible to manipulate fertilization and embryogenesis. Immature embryos are the most promising for in vitro cultivation. Their maturation and germination proceed within seven days of cultivation. When zygotic embryos were cultivated, adventitious buds were formed from cells at the cotyledon base and tips. When adventitious buds were subcultivated on a medium containing benzylaminopurine and naphthylacetic acid, organogenic callus and shoots were formed. Thus, cultivation of megagametophytes and embryos of the Siberian stone pine led to the completion of embryogenesis and formation of viable of seedlings.
Scopus,
Полный текст
Держатели документа:
Sukachev Institute of Forestry, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia.
Доп.точки доступа:
Tret'iakova, I.N.; Novoselova, N.V.
INVITRO CULTIVATION OF TISSUES FROM SEEDLINGS OF 3 SPECIES OF PINE
[Text] / V. V. SKRIPACHENKO // SOVIET PLANT PHYSIOLOGY. - 1982. - Vol. 29, Is. 1. - P162-167. - Cited References: 11
. - 6. - ISSN 0038-5719
РУБ Plant Sciences
WOS
Доп.точки доступа:
SKRIPACHENKO, V.V.
WOS
Доп.точки доступа:
SKRIPACHENKO, V.V.
Potential influence of climate-induced vegetation shifts on future land use and associated land carbon fluxes in Northern Eurasia
[] / D. W. Kicklighter [et al.] // Environ.Res.Lett. - 2014. - Vol. 9, Is. 3. - Ст. 035004, DOI 10.1088/1748-9326/9/3/035004
. - ISSN 1748-9318
Кл.слова (ненормированные):
biofuels -- carbon sequestration -- climate policy -- climatechange effects -- economic feedbacks -- landcover change -- landuse change -- Carbon sequestration -- Carbon-sink activity -- Climate policy -- Climate stabilization -- Ecosystem metabolisms -- Land-cover change -- Land-use change -- Unintended consequences -- Biofuels -- Carbon -- Cultivation -- Expansion -- Land use -- Vegetation -- Climate change
Аннотация: Climate change will alter ecosystem metabolism and may lead to a redistribution of vegetation and changes in fire regimes in Northern Eurasia over the 21st century. Land management decisions will interact with these climate-driven changes to reshape the region's landscape. Here we present an assessment of the potential consequences of climate change on land use and associated land carbon sink activity for Northern Eurasia in the context of climate-induced vegetation shifts. Under a 'business-as-usual' scenario, climate-induced vegetation shifts allow expansion of areas devoted to food crop production (15%) and pastures (39%) over the 21st century. Under a climate stabilization scenario, climate-induced vegetation shifts permit expansion of areas devoted to cellulosic biofuel production (25%) and pastures (21%), but reduce the expansion of areas devoted to food crop production by 10%. In both climate scenarios, vegetation shifts further reduce the areas devoted to timber production by 6-8% over this same time period. Fire associated with climate-induced vegetation shifts causes the region to become more of a carbon source than if no vegetation shifts occur. Consideration of the interactions between climate-induced vegetation shifts and human activities through a modeling framework has provided clues to how humans may be able to adapt to a changing world and identified the trade-offs, including unintended consequences, associated with proposed climate/energy policies. © 2014 IOP Publishing Ltd.
Scopus,
WOS
Держатели документа:
Ecosystems Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, United States
Joint Program on the Science and Policy of Global Change, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States
Department of Earth, Atmospheric and Planetary Sciences, Purdue University, 550 Stadium Mall Drive, West Lafayette, IN 47907, United States
VN Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Kicklighter, D.W.; Cai, Y.; Zhuang, Q.; Parfenova, E.I.; Paltsev, S.; Sokolov, A.P.; Melillo, J.M.; Reilly, J.M.; Tchebakova, N.M.; Lu, X.
Кл.слова (ненормированные):
biofuels -- carbon sequestration -- climate policy -- climatechange effects -- economic feedbacks -- landcover change -- landuse change -- Carbon sequestration -- Carbon-sink activity -- Climate policy -- Climate stabilization -- Ecosystem metabolisms -- Land-cover change -- Land-use change -- Unintended consequences -- Biofuels -- Carbon -- Cultivation -- Expansion -- Land use -- Vegetation -- Climate change
Аннотация: Climate change will alter ecosystem metabolism and may lead to a redistribution of vegetation and changes in fire regimes in Northern Eurasia over the 21st century. Land management decisions will interact with these climate-driven changes to reshape the region's landscape. Here we present an assessment of the potential consequences of climate change on land use and associated land carbon sink activity for Northern Eurasia in the context of climate-induced vegetation shifts. Under a 'business-as-usual' scenario, climate-induced vegetation shifts allow expansion of areas devoted to food crop production (15%) and pastures (39%) over the 21st century. Under a climate stabilization scenario, climate-induced vegetation shifts permit expansion of areas devoted to cellulosic biofuel production (25%) and pastures (21%), but reduce the expansion of areas devoted to food crop production by 10%. In both climate scenarios, vegetation shifts further reduce the areas devoted to timber production by 6-8% over this same time period. Fire associated with climate-induced vegetation shifts causes the region to become more of a carbon source than if no vegetation shifts occur. Consideration of the interactions between climate-induced vegetation shifts and human activities through a modeling framework has provided clues to how humans may be able to adapt to a changing world and identified the trade-offs, including unintended consequences, associated with proposed climate/energy policies. © 2014 IOP Publishing Ltd.
Scopus,
WOS
Держатели документа:
Ecosystems Center, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, United States
Joint Program on the Science and Policy of Global Change, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, United States
Department of Earth, Atmospheric and Planetary Sciences, Purdue University, 550 Stadium Mall Drive, West Lafayette, IN 47907, United States
VN Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Kicklighter, D.W.; Cai, Y.; Zhuang, Q.; Parfenova, E.I.; Paltsev, S.; Sokolov, A.P.; Melillo, J.M.; Reilly, J.M.; Tchebakova, N.M.; Lu, X.
Somatic embryogenesis in Pinus pumila and productivity of embryogenic lines during long-term cultivation in vitro
[Text] / I. N. Tret'yakova, D. N. Shuvaev // Russ. J. Dev. Biol. - 2015. - Vol. 46, Is. 5. - P276-285, DOI 10.1134/S1062360415050070. - Cited References:35
. - ISSN 1062-3604. - ISSN 1608-3326
РУБ Developmental Biology
Аннотация: Zygotic embryos and megagametophytes of Pinus pumila for cultivation in vitro were transferred in 1/2 LV medium supplemented with growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (6-BAP) to induce somatic embryogenesis. Four stably proliferating cell lines from two genotypes were derived. The cell lines differed in the number of globular somatic embryos and the weight of embryogenic calli. Cells of these lines were multiplied as a result of somatic polyembryogenesis via cleavage. In the nutrient medium for maturation, mature somatic embryos were obtained. However, somatic embryos of not all embryogenic cell lines reached maturation. In this study, plantlets were obtained in an in vitro culture for the first time.
WOS,
Scopus,
Статья
Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia.
Доп.точки доступа:
Tret'yakova, I. N.; Shuvaev, D. N.
Рубрики:
MATURE ZYGOTIC EMBRYOS
LOBLOLLY-PINE
CLONAL PROPAGATION
SEED
Кл.слова (ненормированные):
Pinus pumila -- zygotic embryos -- megagametophytes -- somatic embryos -- proliferating embryogenic lines -- in vitro
MATURE ZYGOTIC EMBRYOS
LOBLOLLY-PINE
CLONAL PROPAGATION
SEED
Кл.слова (ненормированные):
Pinus pumila -- zygotic embryos -- megagametophytes -- somatic embryos -- proliferating embryogenic lines -- in vitro
Аннотация: Zygotic embryos and megagametophytes of Pinus pumila for cultivation in vitro were transferred in 1/2 LV medium supplemented with growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (6-BAP) to induce somatic embryogenesis. Four stably proliferating cell lines from two genotypes were derived. The cell lines differed in the number of globular somatic embryos and the weight of embryogenic calli. Cells of these lines were multiplied as a result of somatic polyembryogenesis via cleavage. In the nutrient medium for maturation, mature somatic embryos were obtained. However, somatic embryos of not all embryogenic cell lines reached maturation. In this study, plantlets were obtained in an in vitro culture for the first time.
WOS,
Scopus,
Статья
Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia.
Доп.точки доступа:
Tret'yakova, I. N.; Shuvaev, D. N.
Wood-destroying properties of porodaedalea niemela m. Fischer and trichoderma atroviride bissett from permafrost area
/ Y. A. Litovka [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2017. - Is. 1. - С. 145-150, DOI 10.14258/jcprm.2017011577
. - ISSN 1029-5151
Кл.слова (ненормированные):
Biodegradation -- Lignocarbohydrate complex -- Phenol oxidase activity -- Porodaedalea niemelaei -- Solid-state cultivation -- Trichoderma atroviride -- Waste wood
Аннотация: Presents the results of a research of wood-destroying properties Porodaedalea niemelaei M. Fischer and Trichoderma atroviride Bissett. The strains were isolated in pure culture from the fruiting body and larch wood (Larix gmelinii (Rupr.) Rupr.) in permafrost. Studied phenol oxidase activity and biodegradation of waste wood at their solid-state fermentation. Under laboratory conditions, fungi colonize deciduous and coniferous substrates in monoculture and co-culture. Bioconversion depends on the enzymatic activity of strains and chemical characteristics of the growth substrate. Maximum of wooddestroying activity observed in strain PnB-13 Porodaedalea niemelaei on sawdust of aspen. The loss of weight of the substrate was 39%; polysaccharide content decreased to 1,6 times in comparison with the initial substrate; lignin - in 1.2 times. The concentration of water-soluble substances increased 1,5 times. P. niemelaei and T. atroviride produce extracellular oxidase of lignolytic complex in the solid-phase cultivation of plant residues. Enzyme activity was 0,15-0,22 u/g.s on sawdust of larch and 0,14-0,18 u/g.s on sawdust of aspen, which is comparable to the phenol oxidase activity of other xylotrophic basidiomycetes (Trametes versicolor, Armillaria mellea s.l., Heterobasidion parviporum).
Scopus,
Смотреть статью
Держатели документа:
Siberian State Aerospace University. Academician M.F. Reshetnev, pr. Mira, 82, Krasnoyarsk, Russian Federation
Institute of Forest. V.N. Sukachev, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/28, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Litvinova, E. A.; Chuprova, N. A.
Кл.слова (ненормированные):
Biodegradation -- Lignocarbohydrate complex -- Phenol oxidase activity -- Porodaedalea niemelaei -- Solid-state cultivation -- Trichoderma atroviride -- Waste wood
Аннотация: Presents the results of a research of wood-destroying properties Porodaedalea niemelaei M. Fischer and Trichoderma atroviride Bissett. The strains were isolated in pure culture from the fruiting body and larch wood (Larix gmelinii (Rupr.) Rupr.) in permafrost. Studied phenol oxidase activity and biodegradation of waste wood at their solid-state fermentation. Under laboratory conditions, fungi colonize deciduous and coniferous substrates in monoculture and co-culture. Bioconversion depends on the enzymatic activity of strains and chemical characteristics of the growth substrate. Maximum of wooddestroying activity observed in strain PnB-13 Porodaedalea niemelaei on sawdust of aspen. The loss of weight of the substrate was 39%; polysaccharide content decreased to 1,6 times in comparison with the initial substrate; lignin - in 1.2 times. The concentration of water-soluble substances increased 1,5 times. P. niemelaei and T. atroviride produce extracellular oxidase of lignolytic complex in the solid-phase cultivation of plant residues. Enzyme activity was 0,15-0,22 u/g.s on sawdust of larch and 0,14-0,18 u/g.s on sawdust of aspen, which is comparable to the phenol oxidase activity of other xylotrophic basidiomycetes (Trametes versicolor, Armillaria mellea s.l., Heterobasidion parviporum).
Scopus,
Смотреть статью
Держатели документа:
Siberian State Aerospace University. Academician M.F. Reshetnev, pr. Mira, 82, Krasnoyarsk, Russian Federation
Institute of Forest. V.N. Sukachev, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/28, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Litvinova, E. A.; Chuprova, N. A.
Wood-destroying properties of Fomitopsis Pinicola (SW.) P. Karst. From middle Siberia
/ Y. A. Litovka [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2018. - Is. 1. - С. 193-199, DOI 10.14258/jcprm.2018012729
. - ISSN 1029-5151
Кл.слова (ненормированные):
Biodegradation -- Cellulase activity -- Fomitopsis pinicola -- Lignocarbohydrate complex -- Solid-state cultivation -- Waste wood -- Wood-destroying (xylotrophic) fungi
Аннотация: Presents the results of a research of wood-destroying properties of the basidiomycete Fomitopsis pinicola (Sw.) P. Karst. The strains were isolated in pure culture from fruiting bodies that grew on the Abies sibirica Ledeb, Pinus sylvestris Ledeb, Pinus sibirica Du Tour and Larix sibirica Ledeb in the forests of the Krasnoyarsk Territory (56°09'-56°22' N; 92°10'- 92°58' E). The cultures screened for growth parameters and the dynamics of enzymatic activity during cultivation on cellulose-containing media. The radial growth rate of Siberian strains on the agar medium and plant substrates is 1,1-3,3 mm / day; the growth coefficient is 10-42. All studied cultures synthesize extracellular cellulolytic enzymes that hydrolyze the sodium salt of carboxymethylcellulose. The enzymatic activity is in the range 0,05-0,39 U / ml with a maximum of 7-9 days. The most active strain of Fp5-15 F. pinicola used for bioconversion of coniferous plant substrates under solid-phase culture conditions. The maximum wood-destroying activity observed on pine and larch wood - the mass loss of substrates was 16 and 20%, respectively. On Pinus sylvestris sawdust, the total content of polysaccharides decreased by 1,2 times due to the fermentolysis of the hardly hydrolyzable fraction (a decrease of 1,4 times); the content of lignin substances did not change significantly. On the sawdust of Larix sibirica, the amount of polysaccharides decreased by 1,3 times in comparison with the initial substrate; lig-nin - in 1,2 times. © 2018 Altai State University. All rights reserved.
Scopus,
Смотреть статью
Держатели документа:
Forest Institute, V.N. Sukachev Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 50-28, Krasnoyarsk, Russian Federation
Siberian State University of Science and Technology, Academician M.F. Reshetnev, Mira ave., 82, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Gazizulina, A. V.; Chuprova, N. A.
Кл.слова (ненормированные):
Biodegradation -- Cellulase activity -- Fomitopsis pinicola -- Lignocarbohydrate complex -- Solid-state cultivation -- Waste wood -- Wood-destroying (xylotrophic) fungi
Аннотация: Presents the results of a research of wood-destroying properties of the basidiomycete Fomitopsis pinicola (Sw.) P. Karst. The strains were isolated in pure culture from fruiting bodies that grew on the Abies sibirica Ledeb, Pinus sylvestris Ledeb, Pinus sibirica Du Tour and Larix sibirica Ledeb in the forests of the Krasnoyarsk Territory (56°09'-56°22' N; 92°10'- 92°58' E). The cultures screened for growth parameters and the dynamics of enzymatic activity during cultivation on cellulose-containing media. The radial growth rate of Siberian strains on the agar medium and plant substrates is 1,1-3,3 mm / day; the growth coefficient is 10-42. All studied cultures synthesize extracellular cellulolytic enzymes that hydrolyze the sodium salt of carboxymethylcellulose. The enzymatic activity is in the range 0,05-0,39 U / ml with a maximum of 7-9 days. The most active strain of Fp5-15 F. pinicola used for bioconversion of coniferous plant substrates under solid-phase culture conditions. The maximum wood-destroying activity observed on pine and larch wood - the mass loss of substrates was 16 and 20%, respectively. On Pinus sylvestris sawdust, the total content of polysaccharides decreased by 1,2 times due to the fermentolysis of the hardly hydrolyzable fraction (a decrease of 1,4 times); the content of lignin substances did not change significantly. On the sawdust of Larix sibirica, the amount of polysaccharides decreased by 1,3 times in comparison with the initial substrate; lig-nin - in 1,2 times. © 2018 Altai State University. All rights reserved.
Scopus,
Смотреть статью
Держатели документа:
Forest Institute, V.N. Sukachev Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 50-28, Krasnoyarsk, Russian Federation
Siberian State University of Science and Technology, Academician M.F. Reshetnev, Mira ave., 82, Krasnoyarsk, Russian Federation
Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Gazizulina, A. V.; Chuprova, N. A.
Cytogenetic Stability of Young and Long-Term Embryogenic Cultures of Larix sibirica
/ O. V. Goryachkina [et al.] // Cytologia. - 2018. - Vol. 83, Is. 3. - P322-328, DOI 10.1508/cytologia.83.323. - Cited References:34. - Authors express sincerely thanks to Dr. Oreshkova N.V., V. N. Sukachev Institute of Forest SB RAS, for supplying of L. sibirica genomic DNA, and Dr. Shcherban A.B., The Federal Research Center Institute of Cytology and Genetics SB RAS for preparation of 5S rDNA probe. The study was funded by Russian Foundation for Basic Research and Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support (project No 16-44-243068), Russian Foundation for Basic Research and Belarus (project 18-54-00010), Investment Foundation FASIE (project 0040522).
. - ISSN 0011-4545
РУБ Cell Biology + Genetics & Heredity
Аннотация: We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91-96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica.
WOS,
Смотреть статью,
Scopus
Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Krasnoyarsk Sci Ctr, Siberian Branch,Fed Res Ctr, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Vavilov Inst Gen Genet, Gubkin Str 3, Moscow 119333, Russia.
Доп.точки доступа:
Goryachkina, Olga V.; Park, Maria E.; Tretyakova, Iraida N.; Badaeva, Ekaterina D.; Muratova, Elena N.; Russian Foundation for Basic Research; Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support [16-44-243068]; Investment Foundation FASIE [0040522]; [18-54-00010]
Рубрики:
SOMATIC EMBRYOGENESIS
SOMACLONAL VARIATION
NORWAY SPRUCE
Кл.слова (ненормированные):
Larix sibirica -- Somatic embryogenesis -- Embryogenic cell line -- Chromosome -- Karyotype -- Aneuploidy -- FISH
SOMATIC EMBRYOGENESIS
SOMACLONAL VARIATION
NORWAY SPRUCE
Кл.слова (ненормированные):
Larix sibirica -- Somatic embryogenesis -- Embryogenic cell line -- Chromosome -- Karyotype -- Aneuploidy -- FISH
Аннотация: We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91-96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica.
WOS,
Смотреть статью,
Scopus
Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Krasnoyarsk Sci Ctr, Siberian Branch,Fed Res Ctr, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Vavilov Inst Gen Genet, Gubkin Str 3, Moscow 119333, Russia.
Доп.точки доступа:
Goryachkina, Olga V.; Park, Maria E.; Tretyakova, Iraida N.; Badaeva, Ekaterina D.; Muratova, Elena N.; Russian Foundation for Basic Research; Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support [16-44-243068]; Investment Foundation FASIE [0040522]; [18-54-00010]
Use of Antimicrobial Peptides Secreted by Trichoderma Micromycetes to Stimulate Embryogenic Cultures of Larix sibirica
/ I. N. Tretyakova [et al.] // Russ. J. Dev. Biol. - 2018. - Vol. 49, Is. 6. - P370-380, DOI 10.1134/S1062360419010053. - Cited References:18. - The study was financially supported by the Russian Foundation for Basic Research, the Government of Krasnoyarsk krai, and the Krasnoyarsk Regional Science Foundation (project nos. 16-44-240509 and 18-44-243004).
. - ISSN 1062-3604. - ISSN 1608-3326
РУБ Developmental Biology
Аннотация: In vitro cultivation of embryogenic cultures of Siberian larch on AI medium supplemented with different concentrations of antimicrobial peptides of two Trichoderma species, T.citrinoviride (strain TYVI 4/11) and T. viride (strain 346), has been performed to achieve a direct antimicrobial effect and initiate the mechanisms of induced resistance (regulatory function of embryogenic cultures) as well as to study the morphogenesis and growth activity of regenerant plants. The experiment was arranged using four cell lines, CL4, CL5, CL6, and CL12, differing in their embryogenic activity. The effect of treatment of cell cultures with Trichoderma peptides was manifested via significant growth stimulation of proliferating embryogenic cell lines, root growth stimulation, and formation of callus excrescences on regenerant plants. No difference between the treated and untreated variants was observed in seedlings. Conceivably, such peptide treatment may provide an increased immunity of soil-grown seedlings in forest nurseries. Study of the effect of biocontrol strains' use in a plantation reforestation to stimulate the growth and development of calluses and embryos and to obtain disease-resistant regenerant plants of conifer species will provide the development of a clonal silviculture, a new trend that recently appeared abroad (Park 2002, 2014).
WOS,
Смотреть статью
Держатели документа:
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Gauze Inst New Antibiot, Moscow 119021, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia.
Доп.точки доступа:
Tretyakova, I. N.; Park, M. E.; Baranova, A. A.; Lisetskaya, I. A.; Shuklina, A. S.; Rogozhin, E. A.; Sadykova, V. S.; Russian Foundation for Basic Research; Government of Krasnoyarsk krai; Krasnoyarsk Regional Science Foundation [16-44-240509, 18-44-243004]
Рубрики:
SOMATIC EMBRYOGENESIS
Кл.слова (ненормированные):
Larix sibirica -- embryo suspension mass -- Trichoderma -- antimicrobial -- peptides
SOMATIC EMBRYOGENESIS
Кл.слова (ненормированные):
Larix sibirica -- embryo suspension mass -- Trichoderma -- antimicrobial -- peptides
Аннотация: In vitro cultivation of embryogenic cultures of Siberian larch on AI medium supplemented with different concentrations of antimicrobial peptides of two Trichoderma species, T.citrinoviride (strain TYVI 4/11) and T. viride (strain 346), has been performed to achieve a direct antimicrobial effect and initiate the mechanisms of induced resistance (regulatory function of embryogenic cultures) as well as to study the morphogenesis and growth activity of regenerant plants. The experiment was arranged using four cell lines, CL4, CL5, CL6, and CL12, differing in their embryogenic activity. The effect of treatment of cell cultures with Trichoderma peptides was manifested via significant growth stimulation of proliferating embryogenic cell lines, root growth stimulation, and formation of callus excrescences on regenerant plants. No difference between the treated and untreated variants was observed in seedlings. Conceivably, such peptide treatment may provide an increased immunity of soil-grown seedlings in forest nurseries. Study of the effect of biocontrol strains' use in a plantation reforestation to stimulate the growth and development of calluses and embryos and to obtain disease-resistant regenerant plants of conifer species will provide the development of a clonal silviculture, a new trend that recently appeared abroad (Park 2002, 2014).
WOS,
Смотреть статью
Держатели документа:
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Gauze Inst New Antibiot, Moscow 119021, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia.
Доп.точки доступа:
Tretyakova, I. N.; Park, M. E.; Baranova, A. A.; Lisetskaya, I. A.; Shuklina, A. S.; Rogozhin, E. A.; Sadykova, V. S.; Russian Foundation for Basic Research; Government of Krasnoyarsk krai; Krasnoyarsk Regional Science Foundation [16-44-240509, 18-44-243004]
Interactive Effects of Land Use and Climate on Soil Organic Carbon Storage in Western Siberian Steppe Soils
/ G. Guggenberger, N. Bischoff, O. Shibistova [et al.] ; ed.: M. . Fruhauf [et al.] // KULUNDA: CLIMATE SMART AGRICULTURE: SOUTH SIBERIAN AGRO-STEPPE AS : SPRINGER INTERNATIONAL PUBLISHING AG, 2020. - P183-199. - (Innovations in Landscape Research), DOI 10.1007/978-3-030-15927-6_13. - Cited References:45. - The paper is based on the results of the research work carried out in the scope of the German-Russian cooperation project KULUNDA. The project is financed by the German Federal Ministry of Education and Research (BMBF; FKZ 01LL0905I). We would like to thank all colleagues from the Russian and German partner institutions for their cooperation and support during the investigations.
. -
РУБ Agricultural Economics & Policy + Green & Sustainable Science &
Аннотация: Soils store much more carbon (C) than all terrestrial plants and the Earth's atmosphere together, and the C exchange between soils and atmosphere largely influences the CO2 contents in the atmosphere. While converting native ecosystems into agricultural land in the past caused a huge historical release of C into the atmosphere, an optimization of the management of agricultural soils offers the possibility of restoring parts of the previously lost C in the soil. However, in this respect, interrelationships of land use and soil management with climate change must be considered. In this chapter, land use and climatic effects on soil organic carbon (SOC) stocks in the large western Siberian grasslands will be evaluated and scenarios of future development of SOC storage will be given. A combination of soil analysis along a climatic gradient from the forest steppe to the dry steppe and a modelling approach with the Lund-Potsdam-Jena managed Land (LPJmL) model revealed, that since their cultivation soils of the Kulunda steppe lost about 20-35% of their organic C. Surprisingly, not only particulate organic C was affected but likewise also organic C located within mineral-organic associations was lost, and the proportion of the lost C is independent from the climatic conditions. Parts of this lost organic C can be restored by abandoning arable land. However, due to political and economic constraints, this does not seem to be likely. Minimum or zero tillage may provide an option to increase the organic C storage in western Siberian steppe soils, but the potential effect may be limited. The LPJmL model simulates a continuing climate-change driven C loss from soil, which corroborates results of soil analysis along the climatic gradient. The management of SOC stock has to be evaluated also for its effect on soil erosion, water deficiency and nutrient shortage.
WOS
Держатели документа:
Leibniz Univ Hannover, Inst Soil Sci, Herrenhauser Str 2, D-30419 Hannover, Germany.
Russian Acad Sci, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia.
Landesamt Bergbau Energie & Geol LBEG, Referat L3-2 Landwirtschaft, D-30655 Hannover, Germany.
Inst Klimafolgenforsch PIK, Telegraphenberg A56, D-14412 Potsdam, Germany.
Russian Acad Sci, Inst Water & Environm Problems, Siberian Branch, Molodezhnaya St 1, Barnaul 656038, Russia.
Univ Kopenhagen, Dept Geosci & Nat Resource Management, Oster Voldgade 10, DK-1350 Copenhagen K, Denmark.
Martin Luther Univ Halle Wittenberg, Soil Sci & Soil Protect, Von Seckendorff Pl 3, D-06120 Halle, Germany.
Доп.точки доступа:
Guggenberger, G.; Bischoff, N.; Shibistova, O.; Muller, C.; Rolinski, S.; Puzanov, A.; Prishchepov, A. V.; Schierhorn, F.; Mikutta, R.; Fruhauf, M... \ed.\; Guggenberger, G... \ed.\; Meinel, T... \ed.\; Theesfeld, I... \ed.\; Lentz, S... \ed.\; German Federal Ministry of Education and Research (BMBF)Federal Ministry of Education & Research (BMBF) [FKZ 01LL0905I]
Рубрики:
AGRICULTURAL SOILS
MATTER
NITROGEN
SEQUESTRATION
TEMPERATE
Кл.слова (ненормированные):
Arable land -- Climate change -- Land use -- LPJ model -- No till -- Soil -- cultivation -- Soil fractions -- Soil management -- Soil organic matter
AGRICULTURAL SOILS
MATTER
NITROGEN
SEQUESTRATION
TEMPERATE
Кл.слова (ненормированные):
Arable land -- Climate change -- Land use -- LPJ model -- No till -- Soil -- cultivation -- Soil fractions -- Soil management -- Soil organic matter
Аннотация: Soils store much more carbon (C) than all terrestrial plants and the Earth's atmosphere together, and the C exchange between soils and atmosphere largely influences the CO2 contents in the atmosphere. While converting native ecosystems into agricultural land in the past caused a huge historical release of C into the atmosphere, an optimization of the management of agricultural soils offers the possibility of restoring parts of the previously lost C in the soil. However, in this respect, interrelationships of land use and soil management with climate change must be considered. In this chapter, land use and climatic effects on soil organic carbon (SOC) stocks in the large western Siberian grasslands will be evaluated and scenarios of future development of SOC storage will be given. A combination of soil analysis along a climatic gradient from the forest steppe to the dry steppe and a modelling approach with the Lund-Potsdam-Jena managed Land (LPJmL) model revealed, that since their cultivation soils of the Kulunda steppe lost about 20-35% of their organic C. Surprisingly, not only particulate organic C was affected but likewise also organic C located within mineral-organic associations was lost, and the proportion of the lost C is independent from the climatic conditions. Parts of this lost organic C can be restored by abandoning arable land. However, due to political and economic constraints, this does not seem to be likely. Minimum or zero tillage may provide an option to increase the organic C storage in western Siberian steppe soils, but the potential effect may be limited. The LPJmL model simulates a continuing climate-change driven C loss from soil, which corroborates results of soil analysis along the climatic gradient. The management of SOC stock has to be evaluated also for its effect on soil erosion, water deficiency and nutrient shortage.
WOS
Держатели документа:
Leibniz Univ Hannover, Inst Soil Sci, Herrenhauser Str 2, D-30419 Hannover, Germany.
Russian Acad Sci, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia.
Landesamt Bergbau Energie & Geol LBEG, Referat L3-2 Landwirtschaft, D-30655 Hannover, Germany.
Inst Klimafolgenforsch PIK, Telegraphenberg A56, D-14412 Potsdam, Germany.
Russian Acad Sci, Inst Water & Environm Problems, Siberian Branch, Molodezhnaya St 1, Barnaul 656038, Russia.
Univ Kopenhagen, Dept Geosci & Nat Resource Management, Oster Voldgade 10, DK-1350 Copenhagen K, Denmark.
Martin Luther Univ Halle Wittenberg, Soil Sci & Soil Protect, Von Seckendorff Pl 3, D-06120 Halle, Germany.
Доп.точки доступа:
Guggenberger, G.; Bischoff, N.; Shibistova, O.; Muller, C.; Rolinski, S.; Puzanov, A.; Prishchepov, A. V.; Schierhorn, F.; Mikutta, R.; Fruhauf, M... \ed.\; Guggenberger, G... \ed.\; Meinel, T... \ed.\; Theesfeld, I... \ed.\; Lentz, S... \ed.\; German Federal Ministry of Education and Research (BMBF)Federal Ministry of Education & Research (BMBF) [FKZ 01LL0905I]
Biochemical composition of vegetative explants and callus pinus sibirica du tour
/ Zh. A. Koh, Yu. A. Litovka, P. V. Makolova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 395-403, DOI 10.14258/JCPRM.2020048530
. - ISSN 1029-5151
Перевод заглавия: Биохимический состав вегетативных эксплантов и каллусов pinus sibirica du tour
Кл.слова (ненормированные):
Biochemical composition -- Callus -- Explants -- In vitro cultivation -- Pinus sibirica -- Sterilization -- Water-soluble proteins
Аннотация: The methods of sterilization of annual shoots Pinus sibirica Du Tour and the conditions for their introduction into in vitro culture were studied. Induction of callusogenesis of aseptically viable explants of P. sibirica proceeds more intensively on the modified Murasige-Skoog medium: hormonal supply of 0.4% kinetin and 0.25% 6-benzylaminopurine; reduced sucrose concentration of 1.5%. The frequency of callus formation was 83%. Close quantitative indicators of extractive substances were established (36 and 33% of absolutely dry weight for callus and explant, respectively); easily hydrolyzable polysaccharides (18 and 16%) and proteins (11 and 10%).Callus P. sibirica has a higher content of ascorbic acid, flavanoids, tocopherols and ash elements compared to explants and a low amount of hard-hydrolyzable polysaccharides, lipids, tannins, pigments, and essential oils.The electrophoretic spectrum of water-soluble callus proteins is represented by eleven fractions: 63% of the total water-soluble proteins are fractions with a molecular weight of 33 kD and above. Fractions with molecular weights of 50 and 62 kD (20 and 17%, respectively) are represented as much as possible.In the explants of P. sibirica, low molecular weight fractions of proteins with molecular masses of 5 kD and lower (59%) predominate. The amino acid composition of calli and explants of P. sibirica is identical and is represented by fifteen individual amino acids. Callus tissue has a higher content of glutamic acid and two hydrophobic amino acids (proline and isoleucine) compared to the vegetative part of the plant and low tyrosine content. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Makolova, P. V.; Shabanova, K. A.; Pavlov, I. N.
Перевод заглавия: Биохимический состав вегетативных эксплантов и каллусов pinus sibirica du tour
Кл.слова (ненормированные):
Biochemical composition -- Callus -- Explants -- In vitro cultivation -- Pinus sibirica -- Sterilization -- Water-soluble proteins
Аннотация: The methods of sterilization of annual shoots Pinus sibirica Du Tour and the conditions for their introduction into in vitro culture were studied. Induction of callusogenesis of aseptically viable explants of P. sibirica proceeds more intensively on the modified Murasige-Skoog medium: hormonal supply of 0.4% kinetin and 0.25% 6-benzylaminopurine; reduced sucrose concentration of 1.5%. The frequency of callus formation was 83%. Close quantitative indicators of extractive substances were established (36 and 33% of absolutely dry weight for callus and explant, respectively); easily hydrolyzable polysaccharides (18 and 16%) and proteins (11 and 10%).Callus P. sibirica has a higher content of ascorbic acid, flavanoids, tocopherols and ash elements compared to explants and a low amount of hard-hydrolyzable polysaccharides, lipids, tannins, pigments, and essential oils.The electrophoretic spectrum of water-soluble callus proteins is represented by eleven fractions: 63% of the total water-soluble proteins are fractions with a molecular weight of 33 kD and above. Fractions with molecular weights of 50 and 62 kD (20 and 17%, respectively) are represented as much as possible.In the explants of P. sibirica, low molecular weight fractions of proteins with molecular masses of 5 kD and lower (59%) predominate. The amino acid composition of calli and explants of P. sibirica is identical and is represented by fifteen individual amino acids. Callus tissue has a higher content of glutamic acid and two hydrophobic amino acids (proline and isoleucine) compared to the vegetative part of the plant and low tyrosine content. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Makolova, P. V.; Shabanova, K. A.; Pavlov, I. N.
Biotechnological aspects of bioconversion of post-extraction biomass and cell culture Orthilia secunda (L.) house with basidiomycetes
/ Zh. A. Koh, Yu. A. Litovka, R. Kh. Enazarov [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 359-369, DOI 10.14258/JCPRM.2020048392
. - ISSN 1029-5151
Перевод заглавия: Биотехнологические аспекты утилизации послеэкстракционной биомассы и клеточной культуры orthilia secunda (L.) house базидиальными грибами
Кл.слова (ненормированные):
Basidiomycetes -- Biodegradation -- Callus tissue -- Orthilia secunda -- Pleurotus eryngii -- Post-extraction residue -- Solid-phase cultivation -- Trametes versicolor
Аннотация: The possibility of bioconversion after the extraction residue of the medicinal plant Orthilia secunda (initial plant biomass and callus after extraction of biologically active substances) by fast growing strains of basidiomycetes Tv2-16K Trametes versicolor and Pe-17T Pleurotus eryngii was studied. The main target products are: a mycelial-plant product with a reduced content of the indigestible fraction and enriched in protein and full-fledged fruit bodies. Fungi colonize plant waste with a radial growth rate of 2.0-2.3 mm / day and a growth rate of 65-77 units. Phenol oxidase activity is 0.7-1.2 units/g·s, depending on the type after the extraction residue. In substrates after biodegradation, the proportion of difficult hydrolysable polysaccharides, easily hydrolysable polysaccharides, and lignin is on average 1.6 times lower than in the original substrate. The content of protein and extractive substances is significantly higher, especially after the extraction residue of O. secunda callus under the influence of the T. versicolor strain Tv2-16K: 12.8 and 24.3%, respectively, of the mass of dry substrate. Fruit bodies of P. eryngii strain Pe-17T were obtained on various compositions of plant substrates. The maximum fruit formation was noted on four-component substrates containing two types after extraction residues of O. secunda, wheat bran, birch or aspen sawdust: the average weight of fruit bodies from one block was 230-236 g; biological efficiency - 46-47.2%. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Enazarov, R. Kh.; Makolova, P. V.; Shimova, Yu. S.; Pochekutov, I. S.; Pavlov, I. N.
Перевод заглавия: Биотехнологические аспекты утилизации послеэкстракционной биомассы и клеточной культуры orthilia secunda (L.) house базидиальными грибами
Кл.слова (ненормированные):
Basidiomycetes -- Biodegradation -- Callus tissue -- Orthilia secunda -- Pleurotus eryngii -- Post-extraction residue -- Solid-phase cultivation -- Trametes versicolor
Аннотация: The possibility of bioconversion after the extraction residue of the medicinal plant Orthilia secunda (initial plant biomass and callus after extraction of biologically active substances) by fast growing strains of basidiomycetes Tv2-16K Trametes versicolor and Pe-17T Pleurotus eryngii was studied. The main target products are: a mycelial-plant product with a reduced content of the indigestible fraction and enriched in protein and full-fledged fruit bodies. Fungi colonize plant waste with a radial growth rate of 2.0-2.3 mm / day and a growth rate of 65-77 units. Phenol oxidase activity is 0.7-1.2 units/g·s, depending on the type after the extraction residue. In substrates after biodegradation, the proportion of difficult hydrolysable polysaccharides, easily hydrolysable polysaccharides, and lignin is on average 1.6 times lower than in the original substrate. The content of protein and extractive substances is significantly higher, especially after the extraction residue of O. secunda callus under the influence of the T. versicolor strain Tv2-16K: 12.8 and 24.3%, respectively, of the mass of dry substrate. Fruit bodies of P. eryngii strain Pe-17T were obtained on various compositions of plant substrates. The maximum fruit formation was noted on four-component substrates containing two types after extraction residues of O. secunda, wheat bran, birch or aspen sawdust: the average weight of fruit bodies from one block was 230-236 g; biological efficiency - 46-47.2%. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Enazarov, R. Kh.; Makolova, P. V.; Shimova, Yu. S.; Pochekutov, I. S.; Pavlov, I. N.
Biotechnological potential of the Siberian strains of basidiomycetes - producers of lignolytic and cellulolytic enzymes
/ Yu. A. Litovka, I. N. Pavlov, P. V. Makolova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 371-383, DOI 10.14258/JCPRM.2020048396
. - ISSN 1029-5151
Перевод заглавия: Биотехнологический потенциал сибирских штаммов базидиальных грибов - продуцентов ферментов лигноцеллюлазного действия
Кл.слова (ненормированные):
Basidiomycetes -- Biodegradation -- Cellulases -- Hydrodynamic activation -- Ligninases -- Plant substrates -- Solid-phase and deep cultivation -- Xylotrophs
Аннотация: The results of a study of the wood-destroying properties of Siberian strains of xylotrophic basidiomycetes (Armillaria, Ganoderma, Fomitopsis, Heterobasidion and Porodaedalea) are presented. The growth parameters and enzymatic activity of the strains were determined during solid-phase and deep cultivation. Fomitopsis pinicola and Ganoderma lucidum are fast-growing fungi on cellulose-, tannin-containing nutrient media, coniferous and deciduous plant substrates (source and hydrodynamically activated). The growth coefficient on media with tannin and Na-carboxymethyl cellulose is more than 45; on plant substrates - more than 30. The strains exhibit a different growth reaction to the preliminary activation of birch sawdust (growth acceleration / growth slowdown / indifference). The maximum wood-destroying activity on wood of A. sibirica noted for the fungi A. borealis, G. tsugae, G. lucidum, F. pinicola, and P. niemelaei. The decrease in substrate mass was 8-11%; the amount of polysaccharides decreased on average 1.4 times mainly due to the fermentolysis of hard-hydrolyzable polysaccharides. High enzymatic activity of fungi observed during solid-phase and deep cultivation with an inducer. The maximum activity of phenol oxidase is characteristic of G. tsugae (1.21 units/g·s); carboxymethyl cellulase - for F.pinicola and G. lucidum strains (11.8 and 10.3 units/ml, respectively); xylanases - for H. abietinum (3.8 u/ml). The maximum accumulation of extracellular protein observed in F. pinicola (0.89 mg/ml). According to the totality of rapid test indicators, quantitative determination of enzymatic activity, growth parameters on lignocellulosic substrates and the degree of wood bioconversion, the most promising producer of lignolytic enzymes in vitro is the Gl4-16A Ganoderma lucidum strain; cellulolytic enzymes - strain Fp6-17 Fomitopsis pinicola. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Institute of Physics L.V. Kirensky, SB, RAS, Akademgorodok, 50/43, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Litovka, Yu. A.; Pavlov, I. N.; Makolova, P. V.; Timofeev, A. A.; Litvinova, E. A.; Vasil'Eva, A. A.; Shabanov, A. V.
Перевод заглавия: Биотехнологический потенциал сибирских штаммов базидиальных грибов - продуцентов ферментов лигноцеллюлазного действия
Кл.слова (ненормированные):
Basidiomycetes -- Biodegradation -- Cellulases -- Hydrodynamic activation -- Ligninases -- Plant substrates -- Solid-phase and deep cultivation -- Xylotrophs
Аннотация: The results of a study of the wood-destroying properties of Siberian strains of xylotrophic basidiomycetes (Armillaria, Ganoderma, Fomitopsis, Heterobasidion and Porodaedalea) are presented. The growth parameters and enzymatic activity of the strains were determined during solid-phase and deep cultivation. Fomitopsis pinicola and Ganoderma lucidum are fast-growing fungi on cellulose-, tannin-containing nutrient media, coniferous and deciduous plant substrates (source and hydrodynamically activated). The growth coefficient on media with tannin and Na-carboxymethyl cellulose is more than 45; on plant substrates - more than 30. The strains exhibit a different growth reaction to the preliminary activation of birch sawdust (growth acceleration / growth slowdown / indifference). The maximum wood-destroying activity on wood of A. sibirica noted for the fungi A. borealis, G. tsugae, G. lucidum, F. pinicola, and P. niemelaei. The decrease in substrate mass was 8-11%; the amount of polysaccharides decreased on average 1.4 times mainly due to the fermentolysis of hard-hydrolyzable polysaccharides. High enzymatic activity of fungi observed during solid-phase and deep cultivation with an inducer. The maximum activity of phenol oxidase is characteristic of G. tsugae (1.21 units/g·s); carboxymethyl cellulase - for F.pinicola and G. lucidum strains (11.8 and 10.3 units/ml, respectively); xylanases - for H. abietinum (3.8 u/ml). The maximum accumulation of extracellular protein observed in F. pinicola (0.89 mg/ml). According to the totality of rapid test indicators, quantitative determination of enzymatic activity, growth parameters on lignocellulosic substrates and the degree of wood bioconversion, the most promising producer of lignolytic enzymes in vitro is the Gl4-16A Ganoderma lucidum strain; cellulolytic enzymes - strain Fp6-17 Fomitopsis pinicola. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Institute of Physics L.V. Kirensky, SB, RAS, Akademgorodok, 50/43, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Litovka, Yu. A.; Pavlov, I. N.; Makolova, P. V.; Timofeev, A. A.; Litvinova, E. A.; Vasil'Eva, A. A.; Shabanov, A. V.
Prospects for deep cultivation of fomitopsis pinicola (Sw.) P. Karst. On hydrodynamic activated plant waste
/ I. N. Pavlov, Yu. A. Litovka, E. A. Litvinova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 385-394, DOI 10.14258/JCPRM.2020048400
. - ISSN 1029-5151
Перевод заглавия: Перспективы глубинного культивирования fomitopsis pinicola (SW.) P. karst. на гидродинамически активированных растительных отходах
Кл.слова (ненормированные):
Basidiomycetes -- Carboxymethyl cellulose -- Cellulolytic enzymes -- Fomitopsis pinicola -- Hydrodynamic activation -- Plant waste -- Solid-phase and deep cultivation -- Xylotrophs
Аннотация: The results of solid-phase and deep cultivation of the Siberian strain Fp6-17 Fomitopsis pinicola (Sw.) P. Karst. are presented. Growth parameters were determined on cellulose-containing agarized media and plant substrates with the addition of a solid phase after hydrodynamic activation of fir needles and sawdust of birch. Radial growth rate 3.0-4.3 mm / day; growth coefficient 37-64. Changes in polysaccharides in activated birch sawdust during fermentation noted, despite the low growth coefficient of the fungus. After hydrodynamic activation, the content of easily hydrolysable polysaccharides increased by 9 %, the proportion of hardly hydrolysable polysaccharides decreased by 8%. After further solid-phase cultivation, the content of easily and hardly hydrolysable polysaccharides decreased by 10 and 23%, respectively. The conditions for the deep cultivation of the strain Fp6-17 on a modified Norkrans medium with microcrystalline cellulose selected. At 26 °C and a pH of 4.6 on the seventh day, the maximum activity of carboxymethyl cellulase was 13.6 units / ml; biomass yield - 16.2 g/l. The optimal composition of the solid phase medium was selected after hydrodynamic processing of fir needles and sawdust of birch with enrichment of (NH4)2SO4. The maximum activity of the enzyme was 14.2 u/ml. With high enzymatic activity in the culture fluid, the formation of small globules with a diameter of up to 5 mm and a smaller amount of biomass is noted. At lower enzyme indices, in most cases a greater increase in biomass recorded due to the formation of large ellipsoid pellets with a fringed edge. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Pavlov, I. N.; Litovka, Yu. A.; Litvinova, E. A.; Petrenko, S. M.; Enazarov, R. Kh.
Перевод заглавия: Перспективы глубинного культивирования fomitopsis pinicola (SW.) P. karst. на гидродинамически активированных растительных отходах
Кл.слова (ненормированные):
Basidiomycetes -- Carboxymethyl cellulose -- Cellulolytic enzymes -- Fomitopsis pinicola -- Hydrodynamic activation -- Plant waste -- Solid-phase and deep cultivation -- Xylotrophs
Аннотация: The results of solid-phase and deep cultivation of the Siberian strain Fp6-17 Fomitopsis pinicola (Sw.) P. Karst. are presented. Growth parameters were determined on cellulose-containing agarized media and plant substrates with the addition of a solid phase after hydrodynamic activation of fir needles and sawdust of birch. Radial growth rate 3.0-4.3 mm / day; growth coefficient 37-64. Changes in polysaccharides in activated birch sawdust during fermentation noted, despite the low growth coefficient of the fungus. After hydrodynamic activation, the content of easily hydrolysable polysaccharides increased by 9 %, the proportion of hardly hydrolysable polysaccharides decreased by 8%. After further solid-phase cultivation, the content of easily and hardly hydrolysable polysaccharides decreased by 10 and 23%, respectively. The conditions for the deep cultivation of the strain Fp6-17 on a modified Norkrans medium with microcrystalline cellulose selected. At 26 °C and a pH of 4.6 on the seventh day, the maximum activity of carboxymethyl cellulase was 13.6 units / ml; biomass yield - 16.2 g/l. The optimal composition of the solid phase medium was selected after hydrodynamic processing of fir needles and sawdust of birch with enrichment of (NH4)2SO4. The maximum activity of the enzyme was 14.2 u/ml. With high enzymatic activity in the culture fluid, the formation of small globules with a diameter of up to 5 mm and a smaller amount of biomass is noted. At lower enzyme indices, in most cases a greater increase in biomass recorded due to the formation of large ellipsoid pellets with a fringed edge. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Pavlov, I. N.; Litovka, Yu. A.; Litvinova, E. A.; Petrenko, S. M.; Enazarov, R. Kh.
Component Composition of the Biodeg-Radation Product of Fallen Leaves by Basidiomycetes Pleurotus Pulmonarius (Strain Pp-3.2)
/ O. O. Mamaeva, E. V. Isaeva, S. R. Loskutov, M. A. Plyashechnik // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 1. - С. 277-285, DOI 10.14258/JCPRM.2021018851
. - ISSN 1029-5151
Кл.слова (ненормированные):
amino acid analysis -- Bioconversion -- chemical composition -- digestibility -- heavy metals -- litter -- nucleic acids -- Pleurotus pulmonarius. -- poplar -- protein feed product
Аннотация: Today, plant waste, including fallen poplar leaves (litter), are a promising raw material for the production of useful prod-ucts using bioconversion, such as protein feed additives. The aim of this study was to study the component composition of the products obtained as a result of the bioconversion of leaf litter. The strain PP-3.2 Pleurotus pulmonarius (Fr.) Quel. In the process of substrate conversion, strain PP-3.2 primarily utilizes extractives and easily hydrolyzable polysaccharides, the amount of which decreases by 44 and 36%, respectively. The total content of polysaccharides is reduced by 20%, lignin substances-9.4%. The loss of substrate mass based on leaf litter was 23%. The product obtained after cultivation contains 28% protein. Analysis of the amino acid composition of the protein showed a high rate of phenylalanine with tyrosine (115), threonine (117.5), valine (110) and isoleucine (105%). Also, the biodegradation product has a high content of glutamic and aspartic acids (11.2 and 25.3% of the total amino acids, respectively). The levels of cadmium, lead and copper revealed as a result of studies do not exceed the maximum permissible concentrations established by the veterinary department. At the same time, there is a high content of iron (166.0 Eg/kg) and zinc (256.7 mg/kg) in the feed product. The digestibility of the product is 54%, the content of nucleic substances is up to 0.3%. Thus, the results obtained show the possibility of using the product of the conversion of poplar fallen leaves as a protein feed additive. © 2021 Altai State University. All rights reserved.
Scopus
Держатели документа:
Reshetnev Siberian State University of Science and Technology, pr. Krasnoyarskii Rabochii, 31, Krasnoyarsk, 660037, Russian Federation
Sukachev Institute of Forest SB RAS, Federal Research Center «Krasnoyarsk Scientific Center SB RAS», Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Mamaeva, O. O.; Isaeva, E. V.; Loskutov, S. R.; Plyashechnik, M. A.
Кл.слова (ненормированные):
amino acid analysis -- Bioconversion -- chemical composition -- digestibility -- heavy metals -- litter -- nucleic acids -- Pleurotus pulmonarius. -- poplar -- protein feed product
Аннотация: Today, plant waste, including fallen poplar leaves (litter), are a promising raw material for the production of useful prod-ucts using bioconversion, such as protein feed additives. The aim of this study was to study the component composition of the products obtained as a result of the bioconversion of leaf litter. The strain PP-3.2 Pleurotus pulmonarius (Fr.) Quel. In the process of substrate conversion, strain PP-3.2 primarily utilizes extractives and easily hydrolyzable polysaccharides, the amount of which decreases by 44 and 36%, respectively. The total content of polysaccharides is reduced by 20%, lignin substances-9.4%. The loss of substrate mass based on leaf litter was 23%. The product obtained after cultivation contains 28% protein. Analysis of the amino acid composition of the protein showed a high rate of phenylalanine with tyrosine (115), threonine (117.5), valine (110) and isoleucine (105%). Also, the biodegradation product has a high content of glutamic and aspartic acids (11.2 and 25.3% of the total amino acids, respectively). The levels of cadmium, lead and copper revealed as a result of studies do not exceed the maximum permissible concentrations established by the veterinary department. At the same time, there is a high content of iron (166.0 Eg/kg) and zinc (256.7 mg/kg) in the feed product. The digestibility of the product is 54%, the content of nucleic substances is up to 0.3%. Thus, the results obtained show the possibility of using the product of the conversion of poplar fallen leaves as a protein feed additive. © 2021 Altai State University. All rights reserved.
Scopus
Держатели документа:
Reshetnev Siberian State University of Science and Technology, pr. Krasnoyarskii Rabochii, 31, Krasnoyarsk, 660037, Russian Federation
Sukachev Institute of Forest SB RAS, Federal Research Center «Krasnoyarsk Scientific Center SB RAS», Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Mamaeva, O. O.; Isaeva, E. V.; Loskutov, S. R.; Plyashechnik, M. A.
Induction of somatic embryogenesis in Siberian spruce (Picea obovata) in in vitro culture
/ I. N. Tretyakova, M. E. Park, A. P. Pakhomova [и др.] // Vestn. Tomsk. Gos. Univ. Biol. - 2021. - Is. 54. - С. 6-20, DOI 10.17223/19988591/54/1. - Cited References:0. - The reported study was carried out within the framework of the basic project IL SB RAS-2021-2025 No. 0356-2021-0009 and partially financed by the Russian Foundation for Basic Research, Government of Krasnoyarsk Krai, Krasnoyarsk Region Science and Technology Support Fund (Project No. 19-44-240009).
. - ISSN 1998-8591. - ISSN 2311-2077
РУБ Biology + Ecology
Кл.слова (ненормированные):
Picea obovata -- in vitro -- callus -- embryogenic cultures -- stage of explant -- development -- nutrient medium -- donor tree -- somatic embryogenesis
Аннотация: The biotechnology of somatic embryogenesis in in vitro culture is the most promising direction in the reproduction of conifers. The use of this technology makes it possible not only to massively propagate the best genotypes of trees, but also serves a model for studying the structural, physiological and molecular and genetic mechanisms of both somatic and zygotic embryogenesis in conifers. The main aim of this research was to obtain embryogenic cultures (ECs) producing somatic embryos and embryonic suspension mass (ESM) of Picea obovata. The studies were carried out in 2014-2019 on 30 Siberian spruce trees growing in the vicinity of the city of Krasnoyarsk. To detect genotypes competent for somatic embryogenesis, new donor trees were selected every year for the experiment. 3-10 cones were collected from each tree at different stages of embryo development: globular embryo (the first decade of July), the initiation stage cotyledons (second decade of July), the stage of developed cotyledons (third decade of July) and mature embryos (August). Sterilized explants (zygotic embryos at different stages of development) were introduced into in vitro culture on basic media DCR (Gupta PK and Durzan DJ, 1985), 1/2LN (Litvay JD et al., 1985), MS (Murashige T and Skoog F, 1962) and AI (Tretyakova IN, 2012). All media were supplemented with myo-inositol - 100 mg/L, casein hydrolyzate - 500-1000 mg/L, L-glutamine - 500 mg/L, sucrose - 30 g/L and agar - 7 g/L. Ascorbic acid at a concentration of 400 mg/L was used as an antioxidant. The level of growth regulators was: 2,4-dichlorophenoxyacetic acid (2,4-D) - 2 mg/L and N-6-benzoaminopurine (BAP) - 1 mg/L. For the proliferation of the ESM, DCR and AI basic media containing 2,4-D (2 mg/L), BAP (0.5 mg/L) and sucrose (20 g/L) were used. The pH was adjusted to pH = 5.8. All culture medium and components were sterilized depending on their termolabile properties. Under aseptic conditions, embryos were removed from megagametophytes and inoculated into nutrient media, 10 explants per flask in 25 replicates. The cultures were incubated in the dark at 24 +/- 1 degrees C. Subcultivation to fresh nutrient medium was carried out every 14 days. To control the quality of cell lines (CL) during subculturing, we performed cytological analyzes using temporary preparations (3-5 preparations for each CL). We evaluated the quality of the embryogenicity of the cultures by the presence of even single structures with pronounced polarity - a globular embryo with a suspensor. The results of the study showed that the induction of callus cultures of Siberian spruce is influenced by such factors as the development stage of the explant, the nutrient medium and the genotype of the donor tree. The introduction of P. obovata immature zygotic embryos into in vitro culture at the stage of the globular embryo, both with megagametophytes and extracted from them, turned out to be ineffective. The induction of callus cultures in Siberian spruce was significantly reduced when mature zygotic embryos were introduced into the culture in vitro. The highest response of explants of Siberian spruce was at the stage of developed cotyledons (See Table 1). In the DCR medium, 90% of explants formed callus (See Table 2). The mineral composition of the media did not significantly affect the induction of callus formation in Siberian spruce. The exception was the MS medium, in which callus cultures were formed only in 41% of explants (See Table 2). The growth of callus cultures was most active in the DCR medium. After 6 months of cultivation, 15-32% of calli remained viable (See Table 2). Cytological analysis of callus cultures showed that they include cells of different types (See Fig. 1 and 2). The first type of cells consisted of elongated cells reaching a length of 10 +/- 3 gm, others consisted of isodiametric cells with a diameter of 60 +/- 3.5 gm. The somatic embryo globule and embryonic tubes were formed from elongated cells. Isodiametric cells were actively dividing and forming callus. Only 3 cell lines (out of 300 cell lines) belonging to two donor trees had an active ability to proliferate. Globular somatic embryos were actively forming in these cell lines (See Fig. 3). An actively proliferating ESM was formed. Thus, we carried out a comprehensive assessment of the factors influencing the induction of somatic embryogenesis in Siberian spruce. The results obtained indicate that for the successful formation of somatic embryos, the determining factor is not only the choice of donor plants, but also the development stage of the explant. We found that the best stage in the development of zygotic embryos when introduced into in vitro culture of Siberian spruce is the stage of immature embryos with formed cotyledons, while the DCR, 1/2LN and AI nutrient medium supplemented with growth regulators (2.4-D and BAP) is optimal.
WOS
Держатели документа:
Russian Acad Sci, VN Sukachev Inst Forest, Siberian Branch, Lab Forest Genet & Breeding, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Siberian Fed Univ, Dept Aquat & Terr Ecosyst, 79 Svobodny Ave, Krasnoyarsk 660041, Russia.
Доп.точки доступа:
Tretyakova, Iraida N.; Park, Maria E.; Pakhomova, Angelica P.; Sheveleva, Irina S.; Muratova, Elena N.; Russian Foundation for Basic Research, Government of Krasnoyarsk Krai [IL SB RAS-2021-2025, 0356-2021-0009]; Krasnoyarsk Region Science and Technology Support Fund [19-44-240009]
Кл.слова (ненормированные):
Picea obovata -- in vitro -- callus -- embryogenic cultures -- stage of explant -- development -- nutrient medium -- donor tree -- somatic embryogenesis
Аннотация: The biotechnology of somatic embryogenesis in in vitro culture is the most promising direction in the reproduction of conifers. The use of this technology makes it possible not only to massively propagate the best genotypes of trees, but also serves a model for studying the structural, physiological and molecular and genetic mechanisms of both somatic and zygotic embryogenesis in conifers. The main aim of this research was to obtain embryogenic cultures (ECs) producing somatic embryos and embryonic suspension mass (ESM) of Picea obovata. The studies were carried out in 2014-2019 on 30 Siberian spruce trees growing in the vicinity of the city of Krasnoyarsk. To detect genotypes competent for somatic embryogenesis, new donor trees were selected every year for the experiment. 3-10 cones were collected from each tree at different stages of embryo development: globular embryo (the first decade of July), the initiation stage cotyledons (second decade of July), the stage of developed cotyledons (third decade of July) and mature embryos (August). Sterilized explants (zygotic embryos at different stages of development) were introduced into in vitro culture on basic media DCR (Gupta PK and Durzan DJ, 1985), 1/2LN (Litvay JD et al., 1985), MS (Murashige T and Skoog F, 1962) and AI (Tretyakova IN, 2012). All media were supplemented with myo-inositol - 100 mg/L, casein hydrolyzate - 500-1000 mg/L, L-glutamine - 500 mg/L, sucrose - 30 g/L and agar - 7 g/L. Ascorbic acid at a concentration of 400 mg/L was used as an antioxidant. The level of growth regulators was: 2,4-dichlorophenoxyacetic acid (2,4-D) - 2 mg/L and N-6-benzoaminopurine (BAP) - 1 mg/L. For the proliferation of the ESM, DCR and AI basic media containing 2,4-D (2 mg/L), BAP (0.5 mg/L) and sucrose (20 g/L) were used. The pH was adjusted to pH = 5.8. All culture medium and components were sterilized depending on their termolabile properties. Under aseptic conditions, embryos were removed from megagametophytes and inoculated into nutrient media, 10 explants per flask in 25 replicates. The cultures were incubated in the dark at 24 +/- 1 degrees C. Subcultivation to fresh nutrient medium was carried out every 14 days. To control the quality of cell lines (CL) during subculturing, we performed cytological analyzes using temporary preparations (3-5 preparations for each CL). We evaluated the quality of the embryogenicity of the cultures by the presence of even single structures with pronounced polarity - a globular embryo with a suspensor. The results of the study showed that the induction of callus cultures of Siberian spruce is influenced by such factors as the development stage of the explant, the nutrient medium and the genotype of the donor tree. The introduction of P. obovata immature zygotic embryos into in vitro culture at the stage of the globular embryo, both with megagametophytes and extracted from them, turned out to be ineffective. The induction of callus cultures in Siberian spruce was significantly reduced when mature zygotic embryos were introduced into the culture in vitro. The highest response of explants of Siberian spruce was at the stage of developed cotyledons (See Table 1). In the DCR medium, 90% of explants formed callus (See Table 2). The mineral composition of the media did not significantly affect the induction of callus formation in Siberian spruce. The exception was the MS medium, in which callus cultures were formed only in 41% of explants (See Table 2). The growth of callus cultures was most active in the DCR medium. After 6 months of cultivation, 15-32% of calli remained viable (See Table 2). Cytological analysis of callus cultures showed that they include cells of different types (See Fig. 1 and 2). The first type of cells consisted of elongated cells reaching a length of 10 +/- 3 gm, others consisted of isodiametric cells with a diameter of 60 +/- 3.5 gm. The somatic embryo globule and embryonic tubes were formed from elongated cells. Isodiametric cells were actively dividing and forming callus. Only 3 cell lines (out of 300 cell lines) belonging to two donor trees had an active ability to proliferate. Globular somatic embryos were actively forming in these cell lines (See Fig. 3). An actively proliferating ESM was formed. Thus, we carried out a comprehensive assessment of the factors influencing the induction of somatic embryogenesis in Siberian spruce. The results obtained indicate that for the successful formation of somatic embryos, the determining factor is not only the choice of donor plants, but also the development stage of the explant. We found that the best stage in the development of zygotic embryos when introduced into in vitro culture of Siberian spruce is the stage of immature embryos with formed cotyledons, while the DCR, 1/2LN and AI nutrient medium supplemented with growth regulators (2.4-D and BAP) is optimal.
WOS
Держатели документа:
Russian Acad Sci, VN Sukachev Inst Forest, Siberian Branch, Lab Forest Genet & Breeding, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Siberian Fed Univ, Dept Aquat & Terr Ecosyst, 79 Svobodny Ave, Krasnoyarsk 660041, Russia.
Доп.точки доступа:
Tretyakova, Iraida N.; Park, Maria E.; Pakhomova, Angelica P.; Sheveleva, Irina S.; Muratova, Elena N.; Russian Foundation for Basic Research, Government of Krasnoyarsk Krai [IL SB RAS-2021-2025, 0356-2021-0009]; Krasnoyarsk Region Science and Technology Support Fund [19-44-240009]
Novel materials for myco-decontamination of cyanide-containing wastewaters through microbial biotechnology
/ I. N. Pavlov, Y. A. Litovka // (1 March 2021 through 5 March 2021 : Trans Tech Publications Ltd, 2021. - Vol. 1037 MSF. - P751-758, DOI 10.4028/www.scientific.net/MSF.1037.751
. -
Кл.слова (ненормированные):
Biodegradation -- Biotechnology -- Cyanide wastewater -- Fusarium oxysporum -- Mycelium-based materials -- Biomass -- Bioremediation -- Biotechnology -- Fungi -- Tailings -- Cyanide wastewater -- Fusarium -- Fusarium oxysporums -- Lignocellulosic material -- Microbial biotechnology -- Mycelium-based material -- Novel materials -- Submerged condition -- Tailing dam -- Wood samples -- Biodegradation
Аннотация: This study examined the effectiveness of decontamination of industrial cyanide-containing water using mycelium-based lignocellulosic materials. These results suggest that fungi biomass and plant substrates can be used successfully in the treatment of wastewater contaminated by cyanide. Fungi were isolated from old wood samples taken from a tailing dam with high cyanide content (more than 20 years in semi-submerged condition). All isolated fungi belonged to the genus Fusarium. Fusarium oxysporum Schltdl is most effective for biodegradation of cyanide-containing wastewaters (even at low temperatures). The most optimal lignocellulosic composition for production of mycelium-based biomaterial for biodegradation of cyanide wastewater consists of a uniform ratio of Siberian pine sawdust and wheat straw. The high efficiency of mycelium-based materials has been experimentally proven in vitro at 15-25 ° C. New fungal biomaterials are provide decrease in the concentration of cyanide ions to 79% (P <0.001). Large-scale cultivation of fungi biomass was carried out by the periodic liquid-phase cultivation. The submerged biomass from bioreactor was used as an inoculum for the production of mycelium-based materials for bioremediation of cyanide wastewater in situ (gold mine tailing). © 2021 Trans Tech Publications Ltd, Switzerland.
Scopus
Держатели документа:
V.N. Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Department of Chemical Technology of Wood and Biotechnology, Reshetnev Siberian State University of Science and Technology, Krasnoyarsk, 660037, Russian Federation
Доп.точки доступа:
Pavlov, I. N.; Litovka, Y. A.
Кл.слова (ненормированные):
Biodegradation -- Biotechnology -- Cyanide wastewater -- Fusarium oxysporum -- Mycelium-based materials -- Biomass -- Bioremediation -- Biotechnology -- Fungi -- Tailings -- Cyanide wastewater -- Fusarium -- Fusarium oxysporums -- Lignocellulosic material -- Microbial biotechnology -- Mycelium-based material -- Novel materials -- Submerged condition -- Tailing dam -- Wood samples -- Biodegradation
Аннотация: This study examined the effectiveness of decontamination of industrial cyanide-containing water using mycelium-based lignocellulosic materials. These results suggest that fungi biomass and plant substrates can be used successfully in the treatment of wastewater contaminated by cyanide. Fungi were isolated from old wood samples taken from a tailing dam with high cyanide content (more than 20 years in semi-submerged condition). All isolated fungi belonged to the genus Fusarium. Fusarium oxysporum Schltdl is most effective for biodegradation of cyanide-containing wastewaters (even at low temperatures). The most optimal lignocellulosic composition for production of mycelium-based biomaterial for biodegradation of cyanide wastewater consists of a uniform ratio of Siberian pine sawdust and wheat straw. The high efficiency of mycelium-based materials has been experimentally proven in vitro at 15-25 ° C. New fungal biomaterials are provide decrease in the concentration of cyanide ions to 79% (P <0.001). Large-scale cultivation of fungi biomass was carried out by the periodic liquid-phase cultivation. The submerged biomass from bioreactor was used as an inoculum for the production of mycelium-based materials for bioremediation of cyanide wastewater in situ (gold mine tailing). © 2021 Trans Tech Publications Ltd, Switzerland.
Scopus
Держатели документа:
V.N. Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Department of Chemical Technology of Wood and Biotechnology, Reshetnev Siberian State University of Science and Technology, Krasnoyarsk, 660037, Russian Federation
Доп.точки доступа:
Pavlov, I. N.; Litovka, Y. A.
Hydrodynamically Activated Sawdust of Common Pine Pinus sylvestris L.-substrate for Cultivation of the Strain G14-16A Ganoderma lucidum
/ V. S. Fedorov, T. V. Ryazanova, Y. A. Litovka [и др.] // J. Sib. Fed. Univ.-Chem. - 2022. - Vol. 15, Is. 1. - С. 90-101, DOI 10.17516/1998-2836-0274. - Cited References:30. - The research was conducted under the governmental task issued by the Ministry of Education and Science of Russia for the "Technology and Equipment for the Chemical Processing of the Plant Biomass Material" project by the Plant Material Deep Conversion Laboratory (topic number FEFE-2020-0016) .
. - ISSN 1998-2836. - ISSN 2313-6049
РУБ Chemistry, Multidisciplinary
Кл.слова (ненормированные):
sawdust of Common pine -- hydrodynamic activation -- bioconversion -- Ganoderma lucidum -- growth dynamics -- chemical composition
Аннотация: The results of a study on the use of sawdust of common pine Pinus sylvestris L. with their preliminary activation in a cavitation water-impact dispersant for 25 minutes as a substrate for cultivating the G14-16A strain of Ganoderma lucid= are presented. it was found that the G14-16A strain of Ganoderma lucidum colonizes the substrate more efficiently with the introduction of (NH4)(2)SO4 and Na2HPO4 salts into its composition. At the same time, the average growth rate of basidiomycete on the substrate without the introduction of salts was 1.39 +/- 0.53 mm/day, and on the substrate with nitrogen salts - 3.22 +/- 0.48 mm/day, and complete fouling of the substrate occurred on day 13. It was found that during the cultivation of the G14-16A strain of Ganoderma lucidum on a substrate with nitrogen salts, the content of extractive substances in it increases from 4.37 % to 6.32 %, where more than 65 % is accounted for by substances extracted with water. There is a decrease in the content of difficult-to-hydrolyze polysaccharides by 13.3 %, and an increase in the non-hydrolyzable part by 2.4 %, which is 6.8 % per organic mass. The content of easily hydrolyzable polysaccharides (LGA) decreases by 2.4 %, but this change is not significant in terms of organic mass and the share of LGA both before and after bioconversion is about 15.7 %. The obtained data can expand the field of use of sawdust, which is a promising direction, both in the chemical and pharmaceutical industries.
WOS
Держатели документа:
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, Krasnoyarsk, Russia.
Доп.точки доступа:
Fedorov, Vladimir S.; Ryazanova, Tatyana, V; Litovka, Yulia A.; Pavlova, Igor N.; Litvinova, Ekaterina A.; Petrunina, Elena A.; Loskutov, Sergey R.; Ermolin, Vladimir N.; Bayandin, Mikhail A.; Ministry of Education and Science of Russia for the "Technology and Equipment for the Chemical Processing of the Plant Biomass Material" project by the Plant Material Deep Conversion Laboratory [FEFE-2020-0016]
Кл.слова (ненормированные):
sawdust of Common pine -- hydrodynamic activation -- bioconversion -- Ganoderma lucidum -- growth dynamics -- chemical composition
Аннотация: The results of a study on the use of sawdust of common pine Pinus sylvestris L. with their preliminary activation in a cavitation water-impact dispersant for 25 minutes as a substrate for cultivating the G14-16A strain of Ganoderma lucid= are presented. it was found that the G14-16A strain of Ganoderma lucidum colonizes the substrate more efficiently with the introduction of (NH4)(2)SO4 and Na2HPO4 salts into its composition. At the same time, the average growth rate of basidiomycete on the substrate without the introduction of salts was 1.39 +/- 0.53 mm/day, and on the substrate with nitrogen salts - 3.22 +/- 0.48 mm/day, and complete fouling of the substrate occurred on day 13. It was found that during the cultivation of the G14-16A strain of Ganoderma lucidum on a substrate with nitrogen salts, the content of extractive substances in it increases from 4.37 % to 6.32 %, where more than 65 % is accounted for by substances extracted with water. There is a decrease in the content of difficult-to-hydrolyze polysaccharides by 13.3 %, and an increase in the non-hydrolyzable part by 2.4 %, which is 6.8 % per organic mass. The content of easily hydrolyzable polysaccharides (LGA) decreases by 2.4 %, but this change is not significant in terms of organic mass and the share of LGA both before and after bioconversion is about 15.7 %. The obtained data can expand the field of use of sawdust, which is a promising direction, both in the chemical and pharmaceutical industries.
WOS
Держатели документа:
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, Krasnoyarsk, Russia.
Доп.точки доступа:
Fedorov, Vladimir S.; Ryazanova, Tatyana, V; Litovka, Yulia A.; Pavlova, Igor N.; Litvinova, Ekaterina A.; Petrunina, Elena A.; Loskutov, Sergey R.; Ermolin, Vladimir N.; Bayandin, Mikhail A.; Ministry of Education and Science of Russia for the "Technology and Equipment for the Chemical Processing of the Plant Biomass Material" project by the Plant Material Deep Conversion Laboratory [FEFE-2020-0016]
Использование высечек из листьев ясеня для изучения фитопатогенных свойств гриба HYMENOSCYPUS FRAXINEUS
[Текст] / Н. В. Пашенова, Л. Г. Серая, Ю. Н. Баранчиков // Сибирский лесной журнал. - 2023. - № 1. - С. 58-69, DOI 10.15372/SJFS20230106
. - ISSN 2311-1410
Кл.слова (ненормированные):
усыхание ясеней -- гриб-возбудитель HYMENOSCYPUS FRAXINEUS -- лабораторный тест для выявления фитопатогенности
Аннотация: Лабораторный метод изучения грибной фитотоксичности на высечках из листьев апробирован на грибе Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz & Hosoya - возбудителе усыхания ясеня ( Fraxinus L.). Использовали 12 культур гриба, происходящие из исходного и инвазийного ареалов возбудителя, и листья ясеней маньчжурского ( Fraxinus mandshurica Rupr.) и пенсильванского ( F. pennsylvanica Marsh.), различающихся по устойчивости к данному фитопатогену. После роста грибов на жидких питательных средах фильтраты культур наносили на высечки из листьев ясеней, помещенные во влажные камеры. Некротизация фотосинтезирующих тканей отмечена после действия экзометаболитов некоторых культур. Крупные некрозы развивались только на высечках из листьев ясеня пенсильванского. Это свидетельствует о том, что данный вид менее устойчив к H. fraxineus по сравнению с ясенем маньчжурским. Географическое происхождение и состав питательной среды не влияли на способность культур вызывать некроз. Анализ результатов указывал на вероятную положительную связь между некротизирующей активностью культуральной жидкости и уровнем урожайности биомассы. Можно предположить, что факторы, индуцирующие некроз, появились в культурах на стационарной стадии роста гриба. Не обнаружено совпадения результатов лабораторных опытов с листовыми высечками и полевых опытов по инокуляции мицелия H. fraxineus в стволы молодых ясеней. Обсуждается дефицит знаний о физиологии H. fraxineus и механизмах взаимодействия этого фитопатогена с хозяином. Сделан вывод о пригодности лабораторного метода с использованием высечек из листьев для изучения факторов фитопатогенности H. fraxineus , действующих при заселении фотосинтетической части кроны у чувствительных видов ясеня
A laboratory method for studying the fungal phytotoxicity with cut-offs from leaves was tested for the fungus Hymenoscyphus fraxineus the causative agent of ash dieback ( Fraxinus L.) disease. We used 12 cultures of the fungus originating from the native and invasive ranges of the pathogen, and leaves of two species of ash Manchurian ( Fraxinus mandshurica Rupr.) and ash green ( F. pennsylvanica Marsh.) that differed in resistance to this phytopathogen. After cultivation of fungi on liquid nutrient media, the cultural filtrates were applied to cut-offs from ash leaves placed in moist chambers. Necrotization of photosynthetic tissues was noted after the action of exometabolites of some cultures. At the same time, large necrosis have developed only on the cut-offs from the leaves of ash green, which corresponds to the known fact that this species is less resistant to H. fraxineus in comparison with of ash Manchurian. The geographical origin and composition of the culture medium did not affect the ability of cultures to induce necrosis. The analysis of the results indicated a probable positive relationship between the necrotizing activity of the culture liquid and the indicators of the crop biomass yield. It can be assumed that the necrosis inducing factors appeared in cultures at the stationary stage of the fungus growth. No concurrence was found between the results of laboratory tests with leaf cut-offs and field experiments on the inoculation of H. fraxineus mycelium into the trunks of young ash trees. The deficiency of knowledge about the H. fraxineus physiology and the mechanisms of interaction of this phytopathogen with the host are discussed. A conclusion was made about the suitability of the laboratory technique with leaf cut-offs for factors of H. fraxineus phytopathogenicity investigation, which act during the colonization of the photosynthetic part of the crown in sensitive ash species
РИНЦ
Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28
Доп.точки доступа:
Серая, Лидия Георгиевна; Seraya, Lidiya Georgievna; Баранчиков, Юрий Николаевич; Baranchikov, Yury Nikolayevich; Pashenova, Natal'ya Veniaminovna
| ГРНТИ |
Кл.слова (ненормированные):
усыхание ясеней -- гриб-возбудитель HYMENOSCYPUS FRAXINEUS -- лабораторный тест для выявления фитопатогенности
Аннотация: Лабораторный метод изучения грибной фитотоксичности на высечках из листьев апробирован на грибе Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz & Hosoya - возбудителе усыхания ясеня ( Fraxinus L.). Использовали 12 культур гриба, происходящие из исходного и инвазийного ареалов возбудителя, и листья ясеней маньчжурского ( Fraxinus mandshurica Rupr.) и пенсильванского ( F. pennsylvanica Marsh.), различающихся по устойчивости к данному фитопатогену. После роста грибов на жидких питательных средах фильтраты культур наносили на высечки из листьев ясеней, помещенные во влажные камеры. Некротизация фотосинтезирующих тканей отмечена после действия экзометаболитов некоторых культур. Крупные некрозы развивались только на высечках из листьев ясеня пенсильванского. Это свидетельствует о том, что данный вид менее устойчив к H. fraxineus по сравнению с ясенем маньчжурским. Географическое происхождение и состав питательной среды не влияли на способность культур вызывать некроз. Анализ результатов указывал на вероятную положительную связь между некротизирующей активностью культуральной жидкости и уровнем урожайности биомассы. Можно предположить, что факторы, индуцирующие некроз, появились в культурах на стационарной стадии роста гриба. Не обнаружено совпадения результатов лабораторных опытов с листовыми высечками и полевых опытов по инокуляции мицелия H. fraxineus в стволы молодых ясеней. Обсуждается дефицит знаний о физиологии H. fraxineus и механизмах взаимодействия этого фитопатогена с хозяином. Сделан вывод о пригодности лабораторного метода с использованием высечек из листьев для изучения факторов фитопатогенности H. fraxineus , действующих при заселении фотосинтетической части кроны у чувствительных видов ясеня
A laboratory method for studying the fungal phytotoxicity with cut-offs from leaves was tested for the fungus Hymenoscyphus fraxineus the causative agent of ash dieback ( Fraxinus L.) disease. We used 12 cultures of the fungus originating from the native and invasive ranges of the pathogen, and leaves of two species of ash Manchurian ( Fraxinus mandshurica Rupr.) and ash green ( F. pennsylvanica Marsh.) that differed in resistance to this phytopathogen. After cultivation of fungi on liquid nutrient media, the cultural filtrates were applied to cut-offs from ash leaves placed in moist chambers. Necrotization of photosynthetic tissues was noted after the action of exometabolites of some cultures. At the same time, large necrosis have developed only on the cut-offs from the leaves of ash green, which corresponds to the known fact that this species is less resistant to H. fraxineus in comparison with of ash Manchurian. The geographical origin and composition of the culture medium did not affect the ability of cultures to induce necrosis. The analysis of the results indicated a probable positive relationship between the necrotizing activity of the culture liquid and the indicators of the crop biomass yield. It can be assumed that the necrosis inducing factors appeared in cultures at the stationary stage of the fungus growth. No concurrence was found between the results of laboratory tests with leaf cut-offs and field experiments on the inoculation of H. fraxineus mycelium into the trunks of young ash trees. The deficiency of knowledge about the H. fraxineus physiology and the mechanisms of interaction of this phytopathogen with the host are discussed. A conclusion was made about the suitability of the laboratory technique with leaf cut-offs for factors of H. fraxineus phytopathogenicity investigation, which act during the colonization of the photosynthetic part of the crown in sensitive ash species
РИНЦ
Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28
Доп.точки доступа:
Серая, Лидия Георгиевна; Seraya, Lidiya Georgievna; Баранчиков, Юрий Николаевич; Baranchikov, Yury Nikolayevich; Pashenova, Natal'ya Veniaminovna