Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / I. N. Tret'yakova [et al.] // Russ. J. Plant Physiol. - 2009. - Vol. 56, Is. 5. - P647-653, DOI 10.1134/S1021443709050100. - Cited References: 35. - This work was supported by the Russian Foundation for Basic Research, project no. 06-04-08040-ofi. . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Androgenesis in vitro in plants is a phenomenon of developmental switching of male generative cells, microspores, from their normal gametophytic to sporophytic pathway. We obtained androgenic callus and embryoids (embryo-like structures derived from microspores) of the conifer plant, Siberian larch (Larix sibirica Ledeb.) in the in vitro culture. The immune-enzyme analysis of the hormonal balance of larch androgenic cultures showed a substantial increase in the content of all phytohormones, especially cytokinins and ABA, as compared with initial explants (microstrobiles). This was evidently related to active cell divisions and embryoid formation. A comparison of androgenic cultures derived from trees nonifested and infested with larch gall midges (Dasineura rozhkovi Main. et Nik.) revealed a cytokinin content increase (by two times) and an ABA content decrease (by two times), which indicates more intense growth of cultures derived from healthy trees. Phytohormone content in the androgenic callus was compared with their accumulation in the embryos of larch seeds harvested from noninfested trees. We concluded that successful growth of androgenic cultures and embryoid formation demand an additional medium supplement with auxins (not more than 0.5 mg/l).

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Держатели документа:
[Tret'yakova, I. N.
Ivanitskaya, A. C.
Barsukova, A. V.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia
[Ivanova, A. N.] Siberian Fed Univ, Krasnoyarsk, Russia

Доп.точки доступа:
Tret'yakova, I.N.; Ivanitskaya, A.C.; Ivanova, A.N.; Barsukova, A.V.; Russian Foundation for Basic Research [06-04-08040-ofi]

/ I. N. Tretyakova [et al.] // Plant Cell Tissue Organ Cult. - 2018, DOI 10.1007/s11240-018-01533-y . - Article in Press. - ISSN 0167-6857
Аннотация: We carried out immunoassay of plant hormones [indoleacetic acid (IAA), cytokinins and abscisic acid (ABA)] in the extracts from explants (megagametophytes and embryos) and from four long-term proliferating embryogenic cell lines of Larix sibirica. Immunohistochemical detection of hormones has also been performed at the stage of initiation of somatic embryogenesis and in embryogenic cultures during the process of somatic embryo morphogenesis. During induction of somatic embryogenesis in tissue culture, the first stage of cell development is manifested in their elongation and polarization. Immunohistochemical analysis of hormones showed localization of IAA at one pole of elongated cells of L. sibirica. The polarity of embryogenic structure development persisted later on. The difference has been shown in the content of hormones between the explants and embryogenic cultures. High level of hormones was detected in the cell line (Cl4), distinguished by great number of globular embryos and high capacity for their maturation. Hybrid line Cl5 (L. sibirica ? L. sukaszewii) formed very short suspensor, accumulated higher amount of ABA in the head of somatic embryos (SEs) than did other lines, while SEs of this cell line did not maturate. Cl6 was characterized by low plant hormones content in proliferating embryogenic cultures and low yield of mature somatic embryos. © 2018, Springer Nature B.V.

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Держатели документа:
Siberian Branch, Federal Research Center «Krasnoyarsk Science Center SB RAS», V.N. Sukachev Institute of Forest, Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk, 660036, Russian Federation
Institute of Biology, Ufa Federal Research Center, Russian Academy of Sciences, Prospect Oktyabrya 69, Ufa, 450054, Russian Federation
Department of Biology, Bashkir State University, Validi 32, Ufa, 450076, Russian Federation

Доп.точки доступа:
Tretyakova, I. N.; Kudoyarova, G. R.; Park, M. E.; Kazachenko, A. S.; Shuklina, A. S.; Akhiyarova, G. R.; Korobova, A. V.; Veselov, S. U.

/ I. N. Tretyakova, A. S. Shuklina, M. E. Park [et al.] // Cytologia. - 2021. - Vol. 86, Is. 1. - P55-60, DOI 10.1508/CYTOLOGIA.86.55 . - ISSN 0011-4545
Аннотация: The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in the cells was studied with immunohistochemical analysis. It was shown that the formation of embryogenic cultures in both species is associated with the content of phytohormones and their localization in cells. In L. sibirica the content of IAA in ESM was about 100 times higher than in non-embryogenic calli. At the same time, a low ABA content was characteristic of embryogenic cultures. Non-embryogenic callus contains an increased content of cytokinins and ABA. The transition of somatic cells to the path of embryogenic development is characterized by elongation of cells, their asymmetric division, and localization of IAA at one of the ends of elongated cells. Nonembryogenic callus consisted of isodiametric, actively dividing cells. © 2021 The Japan Mendel Society.

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Держатели документа:
Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Federal Research Center Krasnoyarsk Science Center SB RAS, Akademgorodok 50/28, Krasnoyarsk, 660036, Russian Federation
State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China
Institute of Biology, Russian Academy of Sciences, Ufa Research Center RAS, pr-t Oktyabrya 69, Ufa, 450054, Russian Federation

Доп.точки доступа:
Tretyakova, I. N.; Shuklina, A. S.; Park, M. E.; Yang, L.; Akhiyarova, G. R.; Kudoyarova, G. R.

/ I. N. Tretyakova, A. S. Shuklina, M. E. Park [et al.] // Cytologia. - 2021. - Vol. 86, Is. 1. - P55-60, DOI 10.1508/cytologia.86.55. - Cited References:24. - The reported study was funded by RFBR, the Government of the Krasnoyarsk Territory and the Regional Science Foundation, project number 19-44-240009. . - ISSN 0011-4545РУБ Cell Biology + Genetics & Heredity

Аннотация: The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in the cells was studied with immunohistochemical analysis. It was shown that the formation of embryogenic cultures in both species is associated with the content of phytohormones and their localization in cells. In L. sibirica the content of IAA in ESM was about 100 times higher than in non-embryogenic calli. At the same time, a low ABA content was characteristic of embryogenic cultures. Non-embryogenic callus contains an increased content of cytokinins and ABA. The transition of somatic cells to the path of embryogenic development is characterized by elongation of cells, their asymmetric division, and localization of IAA at one of the ends of elongated cells. Non-embryogenic callus consisted of isodiametric, actively dividing cells.

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Держатели документа:
Russian Acad Sci, Fed Res Ctr, Sukachev Inst Forest, Siberian Branch,Krasnoyarsk Sci Ctr SB RAS, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.
Northeast Forestry Univ, Sch Forestry, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China.
Russian Acad Sci, Ufa Res Ctr RAS, Inst Biol, Pr T Oktyabrya 69, Ufa 450054, Russia.

Доп.точки доступа:
Tretyakova, Iraida N.; Shuklina, Alla S.; Park, Maria E.; Yang, Ling; Akhiyarova, Guzel R.; Kudoyarova, Guzel R.; RFBRRussian Foundation for Basic Research (RFBR); Government of the Krasnoyarsk Territory; Regional Science Foundation [19-44-240009]