Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ G. F. Antonova [et al.] // Russ. J. Dev. Biol. - 2012. - Vol. 43, Is. 4. - P199-208, DOI 10.1134/S1062360412040029. - Cited References: 51. - This work was supported by the Russian Foundation for Basic Research, project no. 06-04-49501. . - 10. - ISSN 1062-3604РУБ Developmental Biology

Аннотация: The content and fractional composition of alcohol soluble phenolic acids (PhA) in cells with different degree maturation and lignification in the course of early and late wood formation in the pine (Pinus sylvestris L.) stem during vegetation were studied. Phenolic compounds (PhC), extracted by 80% ethanol, were divided into free and bound fractions of PhA. In turn, the esters and ethers were isolated from bound PhA. The contents of all substances were calculated per dry weight and per cell. Considerable differences have been found to exist in both the contents and the composition of the fractions PhA on successive stages of tracheid maturation of early and late xylem. Early wood tracheids at all secondary wall thickening steps contained PhC less and free PhA more than late wood tracheids. Throughout earlywood tracheid maturation, the pool of free PhA per cell declined at the beginning of lignification and then increased gradually while that of bound PhA decreased. The maturation of late wood tracheids were accompanied by the rise of free PhA pool and the diminution of bound PhA pool. In the composition of bound PhA, the ethers were always dominant, and the amount of that in earlywood cells was less than in latewood cells. The cells of early xylem at all steps of maturation contained more of esters. The sum total of free hydroxycinnamic acids, precursors of monolignols, gradually decreased during early xylem lignification as the result of the reduction of the pools of p-coumaric, caffeic, ferulic and synapic acids, while that of their esters rised. In the course of late xylem lignification, the pools of free p-coumaric, ferulic and, especially, synapic acids increased. Simultaneously, the amount of ferulic acid ester and synapic acid ether increased too. According to the data, lignin biosynthesis in early xylem and late xylem occurs with different dynamics and the structure of lignins of two xylem types might be different too.

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[Antonova, G. F.
Varaksina, T. N.
Zheleznichenko, T. V.
Stasova, V. V.] Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia

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Antonova, G.F.; Varaksina, T.N.; Zheleznichenko, T.V.; Stasova, V.V.

[Text] / G. F. Antonova, T. V. Zheleznichenko, V. V. Stasova // Russ. J. Dev. Biol. - 2011. - Vol. 42, Is. 4. - P238-246, DOI 10.1134/S1062360411020032. - Cited References: 48. - This work was supported by the Russian Foundation for Basic Research, project no. 06-04-49501. . - 9. - ISSN 1062-3604РУБ Developmental Biology

Аннотация: The content and composition of alcohol soluble phenolic acids (PhAs) were studied during cell xylem growth in course of wood annual increment formation in the trunks of Scots pine. Cells of the cambium zone, two stages of expansion growth, and outset of secondary thickening zone (before lignification) within the period of formation of early wood xylem were subsequently isolated from trunk segments of 25-year-old trees with constant anatomical and histochemical control. The amount of free and bound forms of phenolic acids extracted from tissues by 80% ethanol, as well as their ethers and esters, were calculated both per dry weight and per cells. The substantial alteration in content, proportion of fractions and composition of acids has been found between the cambium zone and the outset of secondary thickening of tracheids, and the character of variation depended on the calculation method. The amount of free and bound PhAs and esters and especially ethers calculated per cell had increased at the first stage of extension growth, reduced at the second, and increased in the outset of secondary wall deposition. The pool of bound acids was more than acids by 2-5 times depending on the stage of development of the cells. Sinapic and ferulic acids dominate among free hydroxycinnamic acids. The composition and the content of hydroxycinnamic acids in esters and ethers also depended on the stage of development of the cells. p-Coumaric and sinapic acids were the main aglycons in ethers in the cambium and sinapic and caffeic acids were in the other stages. The esters from cambium included mostly p-coumaric acid and those at other stages of development were sinapic and ferulic acids. The esters included benzoic acid at the first stages of growth. The pool of these esters decreased from the first phase of growth until the outset of cell wall thickening. The level of free benzoic acid increased respectively.

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Держатели документа:
[Antonova, G. F.
Zheleznichenko, T. V.
Stasova, V. V.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

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Antonova, G.F.; Zheleznichenko, T.V.; Stasova, V.V.

/ G. F. Antonova // : Nova Science Publishers, Inc., 2016. - P1-30 . -
Аннотация: The content and fractional composition of alcohol soluble phenolic acids (PhAs), in particular of hydroxycinnamic acids (HCAs), at consecutive stages of cell differentiation during earlywood and latewood formation in Pinus sylvestris L. stems were studied. Phenolic acids were divided into free and bound fractions. In turn, the esters and ethers were isolated from bound PhAs. The bound acids dominated in PhAs but the amounts of the ethers and esters were different at the stages of growth and maturation of early- and latewood cells. The content of free fraction changed also during cell differentiation. The content coffeic, p-coumaric, ferulic and sinapic acids into the each of the fractions were assessed. Individual HCAs and benzoic acid changed in dependence on the stages of growth expansion and maturation/lignification and the type of forming wood. The data were discussed in the relation to cell growth and xylem lignification during annual ring wood formation. © 2017 by Nova Science Publishers, Inc. All rights reserved.

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Держатели документа:
VN Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Antonova, G. F.

/ G. F. Antonova [et al.] // Wood Sci Technol. - 2019. - Vol. 53, Is. 4. - P927-952, DOI 10.1007/s00226-019-01108-w . - ISSN 0043-7719
Аннотация: Lignification of earlywood and latewood during annual ring formation in Scots pine stem in the season occurs with opposite dynamics. The subject of this study was to examine the structure of lignin deposited at successive stages of secondary cell wall maturation of these two wood types. Lignin specimens derived from developing cells by the thioglycolic acid were analyzed by the methods of alkaline oxidation, alkaline and acid hydrolysis and IR Fourier spectroscopy. The composition of lignin structural subunits was found to change at each stage of lignification in dependence of the forming wood type. The molar ratio of p-hydroxyphenyl, guaiacyl and syringyl subunits in polymer was modified in earlywood and latewood oppositely. In the course of earlywood cell maturation, syringyl subunits in lignin increased in parallel with p-hydroxyphenyl units. During latewood lignification, syringyl and p-hydroxyphenyl subunits in lignin structure decreased, whereas guaiacyl subunits increased as secondary cell wall maturation. In lignin isolated from both wood types, the ether-bound carbohydrates were more than by an order of magnitude of ester-bound carbohydrates. In early xylem, the content of ether-bound carbohydrates practically did not change during lignin deposition, whereas carbohydrates with ester linkages gradually decreased toward mature xylem. Lignification in latewood was accompanied by the decline of both ester-linked and ether-linked carbohydrates with a sharp increase in the latter in mature xylem. Hemicelluloses, included in such bonds, contained mainly the residues of xylose and arabinose. The composition and the content of hydroxycinnamic acids, taking part in these linkages, also changed in dependence of lignification steps and wood type. The data received are in agreement with the alterations in absorption IR spectra of lignins isolated from the cells at the beginning of lignification and mature xylem. The reasons for the differences in the lignin structure during earlywood and latewood development are discussed. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

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Держатели документа:
VN Sukachev Institute of Forest, SB RAS Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Solid State Physics RAS, 2 Academician Ossipyan Str., Chernogolovka, Moscow District, 142432, Russian Federation

Доп.точки доступа:
Antonova, G. F.; Varaksina, T. N.; Zheleznichenko, T. V.; Bazhenov, A. V.

/ N. V. Semenova, V. N. Shmakov, M. E. Park [и др.] // Biol. Membr. - 2020. - Vol. 37, Is. 3. - С. 215-223, DOI 10.31857/S0233475520020127. - Cited References:41 . - ISSN 0233-4755РУБ Cell Biology

Аннотация: It was found that glycerides were dominant for both types of cell lines. It was found that triglycerides (TG) and 1,2-diglycerides (1,2-DG) accumulated 1.5-3 times more actively in embryogenic cell lines, which was probably connected with the necessity of the involvement of TG and 1,2-DG in the processes of tissue differentiation. A high relative content of free fatty acids (FFA) was also characteristic of the embryogenic lines. The found differences in the ratio of sterols to their ethers (the content of sterol esters was 1.5-3 times lower in embryogenic cell lines) can indicate that sterols get activated from their inert form to contribute to the embryogenesis processes at a stage of embryo formation.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Siberian Inst Plant Physiol & Biochem, Irkutsk 664033, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Semenova, N., V; Shmakov, V. N.; Park, M. E.; Tretyakova, I. N.; Konstantinov, Yu M.; Dudareva, L., V