Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ O. V. Goryachkina [et al.] // Plant Syst. Evol. - 2013. - Vol. 299, Is. 2. - P471-479, DOI 10.1007/s00606-012-0737-y. - Cited References: 36. - The authors thank Aleksander P. Isaev Ph.D. (Institute for Biological Problems of Cryolithozone SB RAS, Yakutsk), Alexey P. Barchenkov Ph.D. (V.N. Sukachev Institute of Forest SB RAS, Krasnoyarsk), and Candagdorj Jamiyansuren Ph.D. (Institute of Botany, Mongolian Academy of Sciences, Ulaanbaatar) for supplying the seed samples. This work was supported by grant no. 76 from the Integration Program of the Siberian Branch of the Russian Academy of Sciences and the Russian Foundation for Basic Research (Projects No. 11-04-00063 and 10-04-90780). . - 9. - ISSN 0378-2697РУБ Plant Sciences + Evolutionary Biology

Аннотация: Karyotypes of three Larix species (L. sibirica, L. gmelinii, and L. cajanderi) were analyzed using fluorescence in situ hybridization (FISH) with 45S and 5S ribosomal RNA gene probes and 4',6-diamidino-2-phenylindole (DAPI) staining. Two major 45S ribosomal DNA (rDNA) loci (per haploid genome) have been observed in the intercalary regions of two metacentric chromosomes, III and IV, of L. sibirica; in addition to them, minor nucleolus organizing regions (NORs) were mapped in pericentromeric regions of chromosomes I, II, VI, and XII. Two closely related species, L. gmelinii and L. cajanderi, showed similar hybridization patterns; both species possessed an additional major locus of 45S rDNA in the distal region of the long arm of submetacentric chromosome VII that is absent in L. sibirica. Only one locus of the 5S rDNA was found in all larch species we studied; it was located in the distal region of the chromosome III short arm, which also carried the major NOR in the opposite arm. This chromosome containing major loci of the two ribosomal RNA gene families can serve as a marker of the genus Larix. The intra- and interspecific karyotype diversity in the genus Larix is discussed.

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Держатели документа:
[Goryachkina, Olga V.
Muratova, Elena N.] Russian Acad Sci, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia
[Badaeva, Ekaterina D.
Zelenin, Alexandr V.] Russian Acad Sci, VA Engelhardt Mol Biol Inst, Moscow 119991, Russia

Доп.точки доступа:
Goryachkina, O.V.; Badaeva, E.D.; Muratova, E.N.; Zelenin, A.V.

/ O. V. Goryachkina [et al.] // Cytologia. - 2018. - Vol. 83, Is. 3. - P322-328, DOI 10.1508/cytologia.83.323. - Cited References:34. - Authors express sincerely thanks to Dr. Oreshkova N.V., V. N. Sukachev Institute of Forest SB RAS, for supplying of L. sibirica genomic DNA, and Dr. Shcherban A.B., The Federal Research Center Institute of Cytology and Genetics SB RAS for preparation of 5S rDNA probe. The study was funded by Russian Foundation for Basic Research and Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support (project No 16-44-243068), Russian Foundation for Basic Research and Belarus (project 18-54-00010), Investment Foundation FASIE (project 0040522). . - ISSN 0011-4545РУБ Cell Biology + Genetics & Heredity

Аннотация: We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91-96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Krasnoyarsk Sci Ctr, Siberian Branch,Fed Res Ctr, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Vavilov Inst Gen Genet, Gubkin Str 3, Moscow 119333, Russia.

Доп.точки доступа:
Goryachkina, Olga V.; Park, Maria E.; Tretyakova, Iraida N.; Badaeva, Ekaterina D.; Muratova, Elena N.; Russian Foundation for Basic Research; Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support [16-44-243068]; Investment Foundation FASIE [0040522]; [18-54-00010]

/ S. N. Kirpotin, T. V. Callaghan, A. M. Peregon [et al.] // Ambio. - 2021, DOI 10.1007/s13280-021-01570-6 . - Article in press. - ISSN 0044-7447
Аннотация: Biological diversity is the basis for, and an indicator of biosphere integrity. Together with climate change, its loss is one of the two most important planetary boundaries. A halt in biodiversity loss is one of the UN Sustainable Development Goals. Current changes in biodiversity in the vast landmass of Siberia are at an initial stage of inventory, even though the Siberian environment is experiencing rapid climate change, weather extremes and transformation of land use and management. Biodiversity changes affect traditional land use by Indigenous People and multiple ecosystem services with implications for local and national economies. Here we review and analyse a large number of scientific publications, which are little known outside Russia, and we provide insights into Siberian biodiversity issues for the wider international research community. Case studies are presented on biodiversity changes for insect pests, fish, amphibians and reptiles, birds, mammals and steppe vegetation, and we discuss their causes and consequences. © 2021, Royal Swedish Academy of Sciences.

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Держатели документа:
Tuvan State University, 36 Lenina St., Kyzyl, Republic of Tuva 667000, Russian Federation
Tomsk State University, 36 Lenina Pr., Tomsk, 634050, Russian Federation
University of Sheffield, Alfred Denny Building, Western Bank, Sheffield, S10 2TN, United Kingdom
Institute of Soil Science and Agrochemistry, Siberian Branch of the Russian Academy of Sciences (ISSA SB RAS), Prospect Akademika Lavrentyeva, 8/2, Novosibirsk, 630090, Russian Federation
Institute of the Biological Problem of the North of the Far Eastern Branch of the RAS, 18 Portovaya St, Magadan, 685000, Russian Federation
Siberian Center of State Research Center for Space Hydrometeorology « Planeta», 30, Sovetskaya St, Novosibirsk, 630099, Russian Federation
Novosibirsk Branch of the Russian Federal Research Institute of Fisheries and Oceanography, 1 Pisareva, Novosibirsk, 630091, Russian Federation
Institute of Monitoring of Climatic and Ecological Systems SB RAS, 10/3 Academichesky Pr, Tomsk, 634055, Russian Federation
Sukachev Institute of Forests, Siberian Branch of the Russian Academy of Sciences, 50-28, Academgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodny Pr., 82A, Krasnoyarsk, 660041, Russian Federation
Joint Directorate of Taimyr Nature Reserves, 22 Talnakhskaya St, Norilsk, 663305, Russian Federation
Tomsk State Pedagogical University, 60 Kievskaya St, Tomsk, 634061, Russian Federation

Доп.точки доступа:
Kirpotin, S. N.; Callaghan, T. V.; Peregon, A. M.; Babenko, A. S.; Berman, D. I.; Bulakhova, N. A.; Byzaakay, A. A.; Chernykh, T. M.; Chursin, V.; Interesova, E. A.; Gureev, S. P.; Kerchev, I. A.; Kharuk, V. I.; Khovalyg, A. O.; Kolpashchikov, L. A.; Krivets, S. A.; Kvasnikova, Z. N.; Kuzhevskaia, I. V.; Merzlyakov, O. E.; Nekhoroshev, O. G.; Popkov, V. K.; Pyak, A. I.; Valevich, T. O.; Volkov, I. V.; Volkova, I. I.

/ S. N. Kirpotin, T. V. Callaghan, A. M. Peregon [et al.] // Ambio. - 2021, DOI 10.1007/s13280-021-01570-6. - Cited References:140. - SK, AP, APyak, ZK and AK are grateful for support of the Russian Science Foundation, Grant 20-67-46,018. The work of AP was carried out according to the State assignment of ISSA SB RAS. The research was partly supported by the State assignment of the Ministry of Science and Higher Education of the Russian Federation (Project No. 0721-2020-0019) and RFBR Grants No. 20-04-00587, 19-04-00312A, 18-45-240003, 18-05-00432. Finally, we thank the EU project INTERACT II GA 730938. We also thank Guest Editor Olga Shaduyko for constructive comments and perspectives. . - Article in press. - ISSN 0044-7447. - ISSN 1654-7209РУБ Engineering, Environmental + Environmental Sciences

Аннотация: Biological diversity is the basis for, and an indicator of biosphere integrity. Together with climate change, its loss is one of the two most important planetary boundaries. A halt in biodiversity loss is one of the UN Sustainable Development Goals. Current changes in biodiversity in the vast landmass of Siberia are at an initial stage of inventory, even though the Siberian environment is experiencing rapid climate change, weather extremes and transformation of land use and management. Biodiversity changes affect traditional land use by Indigenous People and multiple ecosystem services with implications for local and national economies. Here we review and analyse a large number of scientific publications, which are little known outside Russia, and we provide insights into Siberian biodiversity issues for the wider international research community. Case studies are presented on biodiversity changes for insect pests, fish, amphibians and reptiles, birds, mammals and steppe vegetation, and we discuss their causes and consequences.

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Держатели документа:
Tuvan State Univ, 36 Lenina St, Kyzyl 667000, Republic Of Tuv, Russia.
Tomsk State Univ, 36 Lenina Pr, Tomsk 634050, Russia.
Univ Sheffield, Alfred Denny Bldg,Western Bank, Sheffield S10 2TN, S Yorkshire, England.
Russian Acad Sci ISSA SB RAS, Inst Soil Sci & Agrochem, Siberian Branch, Prospect Akad Lavrentyeva 8-2, Novosibirsk 630090, Russia.
Siberian Ctr State Res Ctr Space Hydrometeorol Pl, 30 Sovetskaya St, Novosibirsk 630099, Russia.
RAS, Inst Biol Problem, North Far Eastern Branch, 18 Portovaya St, Magadan 685000, Russia.
Russian Fed Res Inst Fisheries & Oceanog, Novosibirsk Branch, 1 Pisareva, Novosibirsk 630091, Russia.
Inst Monitoring Climat & Ecol Syst SB RAS, 10-3 Acad Sky Pr, Tomsk 634055, Russia.
Russian Acad Sci, Sukachev Inst Forests, Siberian Branch, 50-28 Academgorodok, Krasnoyarsk 5028, Russia.
Siberian Fed Univ, Svobodny Pr 82A, Krasnoyarsk 660041, Russia.
Joint Directorate Taimyr Nat Reserves, 22 Talnakhskaya St, Norilsk 663305, Russia.
Tomsk State Pedag Univ, 60 Kievskaya St, Tomsk 634061, Russia.

Доп.точки доступа:
Kirpotin, Sergey N.; Callaghan, Terry V.; Peregon, Anna M.; Babenko, Andrei S.; Berman, Daniil I.; Bulakhova, Nina A.; Byzaakay, Arysia A.; Chernykh, Tatiana M.; Chursin, Vladislav; Interesova, Elena A.; Gureev, Sergey P.; Kerchev, Ivan A.; Kharuk, Viacheslav I.; Khovalyg, Aldynai O.; Kolpashchikov, Leonid A.; Krivets, Svetlana A.; Kvasnikova, Zoya N.; Kuzhevskaia, Irina V.; Merzlyakov, Oleg E.; Nekhoroshev, Oleg G.; Popkov, Viktor K.; Pyak, Andrei I.; Valevich, Tatyana O.; Volkov, Igor V.; Volkova, Irina I.; Russian Science FoundationRussian Science Foundation (RSF) [20-67-46,018]; Ministry of Science and Higher Education of the Russian Federation [0721-2020-0019]; RFBRRussian Foundation for Basic Research (RFBR) [20-04-00587, 19-04-00312A, 18-45-240003, 18-05-00432]; EU project INTERACT II [GA 730938]

[Текст] : научное издание / Е. Н. Муратова, Т. С. Седельникова, О. В. Горячкина, А. В. Пименов // сибирский экологический журнал. - 2023. - Т. 30, № 5. - С. 591-602, DOI 10.15372/SEJ20230502 . - ISSN 0869-8619

ГРНТИ

68.47.03

Аннотация: Обобщены результаты кариологических и цитогенетических исследований в популяциях видов хвойных семейств Pinaceae и Cupressaceae из экстремальных условий произрастания. У хвойных, произрастающих у южной и северной границ ареалов на экологическом пределе распространения, в антропогенно нарушенных экосистемах, в условиях интродукции, выявлена хромосомная изменчивость, обнаружены геномные и хромосомные мутации различного типа. Отмечены высокая частота изменений числа хромосом (миксоплоидия, анеуплоидия и полиплоидия) и их морфологии. Найдены различные типы хромосомных аномалий (кольцевые и полицентрические хромосомы, фрагменты, множественные хромосомные аномалии), агглютинация хромосом, появление В-хромосом, нарушения митоза и мейоза. С использованием классических методов и флуоресцентной гибридизации in situ (FISH) проведены исследования полиморфизма нуклеолярных районов хромосом, выявлены особенности локусов генов 5S и 45S рибосомной РНК.
Results of karyological and cytogenetical studies in populations of conifer species of Pinaceae and Cupressaceae families from extreme conditions are summarized. In conifers growing in southern and northern borders of areas, on ecological limits of distribution, in anthropogenically disturbed ecosystems, at the introduction, chromosome variability is revealed. A high frequency of changes in the number of chromosomes (mixoploidy, aneuploidy and polyploidy) and their morphology were noted. Different types of chromosome anomalies (ring and polycentric chromosomes, fragments, multiple chromosome irregularities), B-chromosomes occurrence, irregularities of mitosis and meiosis are found. Using classical methods and fluorescent in situ hybridization (FISH), studies of the polymorphism of the nucleolar regions of chromosomes were carried out, and features of the loci of the 5S and 45S ribosomal RNA genes were revealed.

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Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Седельникова, Тамара Станиславовна; Sedel'nikova, Tamara Stanislavovna; Горячкина, Ольга Викторовна; Пименов, Александр Владимирович; Pimenov, Alexandr Vladimirovich; Muratova, Elena Nikolayevna