Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ E. V. Bazhina, P. I. Aminev // Russ. J. Ecol. - 2012. - Vol. 43, Is. 2. - P101-106, DOI 10.1134/S1067413612020038. - Cited References: 34. - This study was supported by the Russian Foundation for Basic Research, project no. 09-04-98000. . - 6. - ISSN 1067-4136РУБ Ecology

Аннотация: Fungal canker caused by Biatorella difformis [Fr.]Rehm. has a deleterious effect on the productivity of Scots pine. Affected trees are characterized by decreased biometric parameters of shoots and needles and impaired pollen germination and tube growth. Their pollen has reduced contents of reserve nutrients and physiologically active substances.

Полный текст

Держатели документа:
[Bazhina, E. V.] Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia
Siberian State Technol Univ, Krasnoyarsk 660049, Russia

Доп.точки доступа:
Bazhina, E.V.; Aminev, P.I.

[Text] / I. N. Tretyakova, N. E. Noskova // Russ. J. Ecol. - 2004. - Vol. 35, Is. 1. - P20-26, DOI 10.1023/B:RUSE.0000011105.90297.07. - Cited References: 28 . - 7. - ISSN 1067-4136РУБ Ecology

Аннотация: Environmental conditions causing stress have a significant effect on the generative organs of Scotch pine. The parameters characterizing pine pollen are subject to significant interannual variations and are closely connected with weather and climatic conditions in the period of pollen formation. Airborne pollutants affect the quality of pine pollen, which is especially apparent in the years favorable for the formation of microspores. The ability of Scotch pine pollen to germinate and form pollen tubes in the regions with different technogenic loads proved to be significantly lower than in tree stands of the background areas.

Полный текст,
WOS

Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Tretyakova, I.N.; Noskova, N.E.

[Text] / I. N. Tretyakova, E. V. Bazhina // Ekol. Bratisl. - 2000. - Vol. 19, Is. 3. - P280-294. - Cited References: 49 . - 15. - ISSN 1335-342XРУБ Ecology

Аннотация: Structure of crown and viability of generative organs of Siberian fir were studied in disturbed forest ecosystems near Baikal Lake. The crown structure changes in damaged trees: a zone of subapical drying appears and apical dominance is lost. Needle chlorosis and necrosis appear and proceed in sprouts of male sexualization. The tree top including the female generative organs does not show degradation symptoms during about 30 years and produces seed cones. Processes of degradation in male generative zone greatly influence on fir tree pollen. Pollen viability of fir trees varied from year to year in different trees but always pollen quality of trees growing in the Khamar-Daban Mts in the air pollution zone was more poor in comparison with the pollen of trees at die Baikal Lake shore. Tree pollen growing in the Khamar-Daban Mts germinates in vitro but pollen tubes are formed short and not viable. Seed quality of fir trees is very low, A lot of seeds are sterile. Empty seeds prevail. The formation of empty seeds was caused by the low pollen germination in ovules and as a whole absence of pollen in ovules. The high sterility of generative organs and especially of Abies sibirica pollen can limit gametic selection and decrease fir diversity in forest ecosystems near Baikal Lake.

Scopus

Держатели документа:
RAS, SB, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Tretyakova, I.N.; Bazhina, E.V.

[Text] / O. V. Masyagina, S. G. Prokushkin, M. Y. Sadilova // Contemp. Probl. Ecol. - 2009. - Vol. 2, Is. 6. - P611-619, DOI 10.1134/S1995425509060198. - Cited References: 30. - This work is partially supported with a grant from Krasnoyarsk Regional Scientific Fund and grant 08-04-00034 from the Russian Foundation for Basic Research, integrated project no. 76. . - 9. - ISSN 1995-4255РУБ Ecology

Аннотация: The influence of optimal and contrasting temperatures on the fractional composition of proteins and respiration of germinating seeds of Gmelin larch (Larix gmelinii (Rupr.) Rupr.) and Siberian larch (Larix sibirica Ledeb.) was revealed. The dominance of globulins (salt-soluble fraction) and insoluble proteins was noted in viable air-dry seeds of larch. Optimal and contrasting temperatures of germination of seeds caused significant changes in the content and dynamics of separate fractions of protein, the contrasting temperatures causing greater changes: increasing in the content of insoluble proteins, intense consumption of globulins at separate stages of germination. Respiration of the seeds during germination at contrasting temperatures also increased more than at an optimal temperature. Thermal regime of germination affected the character of relations between the respiration rate and contents of different protein fractions.

Полный текст,
WOS,
Scopus

Держатели документа:
[Masyagina, O. V.
Prokushkin, S. G.
Sadilova, M. Yu.] Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Masyagina, O.V.; Prokushkin, S.G.; Sadilova, M.Y.; Krasnoyarsk Regional Scientific Fund; Russian Foundation for Basic Research [08-04-00034]

/ N. V. Ekimova, E. N. Muratova, P. P. Silkin // Russian Journal of Genetics: Applied Research. - 2012. - Vol. 2, Is. 2. - P105-109, DOI 10.1134/S2079059712020037 . - ISSN 2079-0597
Аннотация: Chromosome numbers for some species of Central Asia steppe shrubs are given: Rhamnus erythroxylon Pall. (Rhamnaceae), Caragana buriatica Peschk. (Fabaceae), Amygdalus pedunculata Pall., Armeniaca sibirica (L.) Lam. (Rosaceae), Atraphaxis pungens (Bieb.) Jaub. et Spach, and A. frutescens (L.) C. Koch (Polygonaceae). Chromosome numbers of some species were determined for the first time. A comparative analysis of adaptive properties of polyploidy and diploid species has been conducted. It was established that natural polyploids are more adaptive to existence in extreme conditions. They possess a high potential for survival and are characterized by a higher level of intraspecific polymorphism, abundant flowering and fruitification, ability to propagate both via generative and vegetative means, and high seed germination. В© 2012 Pleiades Publishing, Ltd.

Scopus,
Полный текст

Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
V.N. Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Ekimova, N.V.; Muratova, E.N.; Silkin, P.P.

/ I. N. Tret'iakova, N. V. Novoselova // Ontogenez. - 2003. - Vol. 34, Is. 4. - С. 282-291 . - ISSN 0475-1450
Аннотация: Seedlings were grown in vitro from fertilized eggs and immature embryos of the Siberian stone pine. Cultivation of megagametophytes on a hormone-containing Murashige-Skoog medium from the egg formation until the globular embryo stage made it possible to manipulate fertilization and embryogenesis. Immature embryos are the most promising for in vitro cultivation. Their maturation and germination proceed within seven days of cultivation. When zygotic embryos were cultivated, adventitious buds were formed from cells at the cotyledon base and tips. When adventitious buds were subcultivated on a medium containing benzylaminopurine and naphthylacetic acid, organogenic callus and shoots were formed. Thus, cultivation of megagametophytes and embryos of the Siberian stone pine led to the completion of embryogenesis and formation of viable of seedlings.

Scopus,
Полный текст

Держатели документа:
Sukachev Institute of Forestry, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia.

Доп.точки доступа:
Tret'iakova, I.N.; Novoselova, N.V.

/ A. V. Kirdyanov [et al.] // Boreas. - 2012. - Vol. 41, Is. 1. - P56-67, DOI 10.1111/j.1502-3885.2011.00214.x . - ISSN 0300-9483
Аннотация: Ongoing climatic changes potentially affect tree-line ecosystems, but in many regions the observed changes are superimposed by human activities. We assessed how the forest-tundra ecotone has changed during the last century in the Putorana Mountains, northern Siberia, an extremely remote and untouched area in Eurasia. A space-for-time approach was used to determine the spatio-temporal dynamics of forest structure and biomass along an altitudinal transect. From the closed larch forest to the upper tree line, the mean age of Larix gmelinii (Rupr.) decreased considerably from 220 to 50 years ago. At the current upper species line, there is a strong and successful germination of larch, with 1500 saplings per hectare, indicating an ongoing filling-in, a densification of a formerly open forest and an upslope shift of the tree-line position (approximately 30 to 50m in altitude during the last century). The forest expansion coincided with large increases in winter precipitation during the 20th century. In contrast, tree growth rates were significantly positively related to summer temperatures, neither of which increased markedly. The total aboveground biomass decreased from approximately 40tha -1 in the closed larch forest to 5tha -1 at the tree line. Our study demonstrates that ongoing climatic changes lead to an upslope expansion of forests in the remote Putorana Mountains, which alters the stand structure and productivity of the forest-tundra ecotone. These vegetation changes are very probably of minor importance for aboveground carbon sequestration, but soil carbon data are needed to estimate the impact of the forest expansion on the total ecosystem carbon storage. В© 2011 The Authors. Boreas В© 2011 The Boreas Collegium.

Scopus

Держатели документа:
V. N. Sukachev Institute of Forest SB RAS, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Swiss Federal Research Institute WSL, Zurcherstrasse 111, CH-8903 Birmensdorf, Switzerland
Siberian Federal University, pr. Svobodny 79, Krasnoyarsk, 660041, Russian Federation
Institute of Plant and Animal Ecology UrB RAS, 8 Marta str. 202, Ekaterinburg, 620144, Russian Federation

Доп.точки доступа:
Kirdyanov, A.V.; Hagedorn, F.; Knorre, A.A.; Fedotova, E.V.; Vaganov, E.A.; Naurzbaev, M.M.; Moiseev, P.A.; Rigling, A.

/ I. N. Tretyakova [et al.] // Russ. J. Plant Physiol. - 2016. - Vol. 63, Is. 6. - P800-810, DOI 10.1134/S1021443716050137 . - ISSN 1021-4437
Аннотация: Morphogenesis and maturation of somatic embryos, ploidy, and genotyping of cell lines (CL) of embryogenic cultures of Larix sibirica Ledeb. in vitro were investigated during 2–6 years. It was revealed that from 2000 to 11103 globular somatic embryos were formed in proliferating CL. However, the ability of somatic embryos to the maturation and germination decreased. Cytogenetic study of embryonal-suspensor masses (ESM) of Larix sibirica demonstrated that cells of long-term cultivated cultures remained diploid. According to microsatellite analysis, proliferating CL of Siberian larch were characterized by weak allelic variability, and cell line 6 and cloned seedlings of this line were genetically stable and corresponded to the donor tree. Embryogenic cell lines composed the collection bank, which will be successfully used for plantation forest growing. © 2016, Pleiades Publishing, Ltd.

Scopus,
Смотреть статью,
WOS

Держатели документа:
Sukachev Institute of Forest, Russian Academy of Science, Siberian Branch, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Park, M. E.; Ivanitskaya, A. S.; Oreshkova, N. V.

/ O. E. Kondakova, I. D. Grodnitskaya // Vestn. Tomsk. Gos. Univ. Biol. - 2018. - Is. 42. - С. 54-68, DOI 10.17223/19988591/42/3 . - ISSN 1998-8591
Аннотация: The microbiological method is applied for the purpose of artificial forest regeneration, as the most effective method of protecting forest planting material grown in forest nurseries. At present, literature data contain many examples of using species and genera of microorganisms belonging to different taxa in order to protect plants. The aim of the research was to establish biological (antagonistic, enzymatic and growth-stimulating) activity of the museum microorganism cultures belonging to different taxonomic groups (bacteria, fungi), and to assess their influence on the growth and development of Scots pine seeds in vitro and a decrease in the number of phytopathogenic fungi. We isolated previously selected microorganisms from the nursery soils; these microorganisms belong to different taxonomic groups, namely, Trichoderma micromycetes (T. harzianum, T. longibrachaitum, and T. lignorum), Streptomyces lateritius bacteria, Bacillus amyloliquefaciens, as well as phytopathogenic Fusarium fungi (F. oxysporum, F. moniliforme, F. proliferatum, F. moniliforme var annullatum, and F. oxysporum B3). Antagonistic activity of microbial strains was determined by the dual culture method, and the presence of enzymatic activity (lipase, proteinase and chitinase) of the tested strains was observed by qualitative express tests. We studied the growth-promoting activity by soaking pine seeds in aqueous suspensions of antagonists (106 spores/ml) (Pegalado, 2000; Cullimore, 2001; Montealegre, 2003; Asaturova, 2012). The results of the research showed that the investigated microorganisms (fungi, actinobacteria and bacteria) are biologically active. The most powerful antagonists were micromycetes of T. harzianum, T. lignorum, and T. longibrachiatum, which are also capable of exhibiting mycophilic properties (hyperparasitism). Thus, T. longibrachiatum showed mycophilia against three strains: F. moniliforme, F. moniliforme var annulatum, and F. oxysporum B3, whereas T. harzianum and T. lignorum did against two: F. moniliforme and F. proliferatum; the degree of phytopathogen inhibition (ID) varied from 30 to 100% (See Table 1). The strain of B. amiloliquefaciens bacterium was less active, the DI was 41.4%, on the average, and the slowest antagonistic properties were exhibited by actinobacterium S. lateritius - 14.8%, on the average. The investigation of the presence of the main hydrolytic enzymes (a hitinaze, a lipase, protease) showed that Trichoderma micromycetes had the average and strong hydrolytic activity (T. harzianum and T. longibrachiatum), and bacteria (S. lateritius, B. amyloliquefaciens) had the average and weak hydrolytic activity (See Table 2). Also, all the investigated strains improved Scots pine seed germination, while the strains of B. amyloliquefaciens and T. longibrachiatum showed the greatest growth-promoting activity (See Figures). Thus, we found that the investigated strains (T. harzianum, T. lignorum, T. longibrachiatum, S. lateritius, and B. amyloliquefaciens) had a high antagonistic activity, and Trichoderma micromycetes revealed the ability for mycoparasitism. The high biological (enzymatic, antagonistic, growth-stimulating) activity of the studied strains of microorganisms makes them effective agents for biological control in forest nurseries. © 2018 Tomsk State University. All rights reserved.

Scopus,
Смотреть статью

Держатели документа:
Laboratory of Microbiology and Ecological Biotechnology, VN Sukachev Institute of Forest, Siberian Branch of the Russian Academy of Sciences, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Kondakova, O. E.; Grodnitskaya, I. D.

/ V. P. Zhelifonova [et al.] // Appl. Biochem. Microbiol. - 2019. - Vol. 55, Is. 3. - P277-283, DOI 10.1134/S0003683819030153. - Cited References:18 . - ISSN 0003-6838. - ISSN 1608-3024РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: Secondary metabolites of the basidiomycetes of Armillaria borealis Marxm. and Korhonen, A.cepistipes Velen., A. gallica Marxm., A. ostoyae (Romagn.) Herink, and A. sinapina Berube and Dessur isolated in Southern Siberia (Krasnoyarsk region and Tyva Republic) and in the Far East (Sikhote-Alin) were studied. Metabolites belonging to the class of protoilludene sesquiterpene aryl esters of the melleolides group have been identified in the species A. borealis, A. cepistipes, and A. sinapina. The strains differ in the spectrum of synthesized melleolides. A. borealis strain 74g synthesized a wider range of melleolides than other strains of the species: melleolides B, C, D and H, melledonals B and C, 5'-O-methylmelledonal, 13-hydroxy-5'-O-methylmelledonal, and armillarinin. It was shown that the composition of the medium influenced the amount of synthesized metabolites. All of the studied strains synthesizing melleolides exhibited a toxicogenic and phytopathogenic effect on seeds and seedlings of Picea abies (L.) H. Karst. and Abies sibirica Ledeb. A significant decrease in seed germination energy, laboratory and ground seed germination, the development of the root system of conifer seedlings was revealed. The maximum inhibitory effect was shown by the culture fluid of A. borealis 74g with the greatest amounts and variety of melleolides in the metabolome profile.

WOS,
Смотреть статью,
Scopus

Держатели документа:
Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Zhelifonova, V. P.; Antipova, T. V.; Litvinova, E. A.; Baskunov, B. P.; Litovka, Yu. A.; Pavlov, I. N.; Kozlovsky, A. G.

[Текст] : статья / В. И. ВОРОНИН, А. П. СОФРОНОВ, Т. И. МОРОЗОВА [и др.] // География и природные ресурсы. - 2019. - № 4. - С. 56-65 . - ISSN 0206-1619
   Перевод заглавия: IN DARK-CONIFEROUS FORESTS ON KHAMAR-DABAN RANGE (SOUTHERN CISBAIKALIA)

УДК

911.2

528.9

632.3

630.443.3

630*561.24

Аннотация: Приводятся данные комплексного исследования причин и масштабов усыхания темнохвойных лесов в геосистемах северного макросклона хр. Хамар-Дабан с 2006 по 2009 г. Причиной усыхания стало заболевание «бактериальная во дянка», вызываемое бактерией Erwinia nimipressuralis Carter. Спусковым механизмом для развития заболевания по служило маловодье, наблюдавшееся в регионе в этот период. Для оценки масштабов повреждения темнохвойных лесов использовался ландшафтный подход. Была создана среднемасштабная карта геосистем северного макросклона хр. Ха мар-Дабан, на которой показано разнообразие геосистем региона уровня классов фаций. Выполнена работа по ранжи рованию лесов по трем степеням пораженности древостоя (от сильной до слабой) и проведена оценка территории их распространения. Основные площади нарушенных лесов сосредоточены в восточной и западной областях Хамар-Даба на, где они занимают среднюю и верхнюю полосы горно-таежного пояса. Наиболее пострадал от заболевания кедровый древостой. Пихта повреждена меньше, но в ряде мест нарушение кедрового и пихтового древостоя сопоставимо. Усыхание елового древостоя не выявлено. Резкое падение скорости прироста у кедрового древостоя приходится на 2006-2009 гг. Кроме этого, отмечается уменьшение содержания питательных веществ в пыльцевых зернах кедра и снижение активности их прорастания, а также негативная трансформация кедровых шишек в виде их избыточного засмоления и недоразвитости, что является одним из диагностических признаков бактериальной водянки. Нарушение развития пыльцы и шишек кедра негативно сказывается на вызревании семян кедра, замедляя лесовозобновление. Однако изучение состояния подроста в пострадавших от бактериальной водянки насаждениях свидетельствует об удовлетворительных в целом процессах восстановления темнохвойных лесов.
Presented are the data from a comprehensive investigation into the causes and extent of dark coniferous forest dieback in geosystems of the northern macroslope of Khamar-Daban Range for the time interval 2006-2009. Forest dieback was caused by bacteria Erwinia nimipressuralis Carter. The disease outbreak was triggered by water scarcity in the region at that period. Land scape approach was used in assessing the magnitude of damage to dark-coniferous forests. The medium-scale map of geosystems was created for the northern macroslope of Khamar-Daban Range, showing the region’s geosystem diversity at the level of classes of facies. The ranking of forests was done according to three degrees of damage to tree stands (from strong to weak), and an assessment was made of the territory covered by them. The main areas of affected forests are concentrated in the eastern and western regions of Khamar-Daban where they occupy the middle and upper parts of the mountain-taiga belt. The Siberian stone pine stands suffered the most from the disease. Fir trees were less affected; in some areas, however, the damage to Siberian stone pine and fir stands is comparable. A dramatic decrease of the rate of radial increment in Siberian stone pine stands corresponds to the time interval 2006-2009. Furthermore, there occurred a decrease in nutrient content in pollen grains of Siberian stone pine and a decrease in of the rate of their germination as well as a negative transformation of Siberian stone pine cones in the form of their excessive resinosis and immaturity, which is one of the diagnostic features of bacterial dropsy. A disturbance to the development of pollen and pine cones negatively affects the ripening of Siberian stone pine seeds thus impeding forest regenera tion. However, a study of the state of the young growth in forest stands affected by bacterial dropsy bears witness to the gener ally satisfactory processes of dark-coniferous forest rehabilitation.

РИНЦ

Держатели документа:
Институт географии им. В.Б. Сочавы СО РАН
Институт леса им. В.Н. Сукачёва СО РАН
Красноярский научный центр СО РАН
Педагогический институт Иркутского государственного университета
Сибирский институт физиологии и биохимии растений СО РАН

Доп.точки доступа:
ВОРОНИН, В.И.; VORONIN V.I.; СОФРОНОВ, А.П.; SOFRONOV A.P.; МОРОЗОВА, Т.И.; MOROZOVA T.I.; ОСКОЛКОВ, В.А.; OSKOLKOV V.A.; Суховольский, Владислав Григорьевич; Soukhovolsky Vladislav Grigor'yevich; КОВАЛЁВ, А.В.; KOVALEV A.V.

/ I. N. Tretyakova, E. A. Rogozhin, M. E. Pak [et al.] // Biol. Bull. - 2020. - Vol. 47, Is. 3. - P225-236, DOI 10.1134/S1062359020030097. - Cited References:38. - This work was conducted within the framework of a Budget Project of Sukachev Institute of Forests, Siberian Branch, Russian Academy of Sciences, Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences"(project no. 0356-2017-0741) and supported by the Russian Foundation for Basic Research, the Government of Krasnoyarsk region, and the Krasnoyarsk Regional Science Foundation within the framework of research project no. 16-44-240509 "The Development of Biotechnology for the Production of Embryogenic Cultures of Siberian Larch Resistant to Fungal Diseases and Pests with the Use of Protective Antimicrobial Peptides in vitro" and no. 18-44-243004 "In vitro Studies of the Effect of Biologically Active Peptides of Plant and Microbial Origin on the Growth and Development of Conifers in Early Ontogenesis." . - ISSN 1062-3590. - ISSN 1608-3059РУБ Biology

Аннотация: The effect of plant antimicrobial peptides on the initiation of callus and embryonal suspensor masses, formation of somatic embryos, and germination of regenerants of Siberian larch has been studied. Protein/peptide extracts isolated fromAmarantus retroflexus(seeds),Nigella sativa(seeds), andElytrigia elongata(spikelets) have been used as objects of plant origin. Peptides have been introduced into the nutrient media at the stage of initiation of embryogenic cultures and somatic embryo germination. The stimulating effect of peptides on the formation of embryogenic cultures of Siberian larch has been found. No other differences in the dynamics of growth in the control and experimental regenerants have been observed. This study is supposed to contribute to enhancing the immunity of the clonal planting stock of Siberian larch.

WOS

Держатели документа:
Russian Acad Sci, Sukachev Inst Forests, Siberian Branch, Div Fed Res Ctr Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia.
Gause Inst New Antibiot, Moscow 119021, Russia.
Tyumen State Univ, Tyumen 625003, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Tretyakova, I. N.; Rogozhin, E. A.; Pak, M. E.; Petukhova, I. A.; Shuklina, A. S.; Pahomova, A. P.; Sadykova, V. S.; Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences [0356-2017-0741]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Government of Krasnoyarsk region; Krasnoyarsk Regional Science Foundation [16-44-240509, 18-44-243004]

/ I. N. Tretyakova, E. A. Rogozhin, M. E. Pak [et al.] // Biol. Bull. - 2020. - Vol. 47, Is. 3. - P225-236, DOI 10.1134/S1062359020030097 . - ISSN 1062-3590
Аннотация: Abstract: The effect of plant antimicrobial peptides on the initiation of callus and embryonal suspensor masses, formation of somatic embryos, and germination of regenerants of Siberian larch has been studied. Protein/peptide extracts isolated from Amarantus retroflexus (seeds), Nigella sativa (seeds), and Elytrigia elongata (spikelets) have been used as objects of plant origin. Peptides have been introduced into the nutrient media at the stage of initiation of embryogenic cultures and somatic embryo germination. The stimulating effect of peptides on the formation of embryogenic cultures of Siberian larch has been found. No other differences in the dynamics of growth in the control and experimental regenerants have been observed. This study is supposed to contribute to enhancing the immunity of the clonal planting stock of Siberian larch. © 2020, Pleiades Publishing, Inc.

Scopus

Держатели документа:
Sukachev Institute of Forests, Siberian Branch, Russian Academy of Sciences, Division of Federal Research Center “Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences”, Krasnoyarsk, 660036, Russian Federation
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russian Federation
Gause Institute of New Antibiotics, Moscow, 119021, Russian Federation
Tyumen State University, Tyumen, 625003, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Tretyakova, I. N.; Rogozhin, E. A.; Pak, M. E.; Petukhova, I. A.; Shuklina, A. S.; Pahomova, A. P.; Sadykova, V. S.

/ F. Gao, C. Peng, H. Wang [et al.] // Forests. - 2020. - Vol. 11, Is. 9. - Ст. 912, DOI 10.3390/F11090912 . - ISSN 1999-4907
Аннотация: Korean pine is the dominant species of Korean pine forests. It is an economically valuable species that yields oil, high-quality timber and nuts, and it offers great prospects for further development. Complete regenerated plants of Korean pine were obtained via somatic embryogenesis using megagametophytes as the explant. The seeds of 27 families of Korean pine were collected to induce embryogenic lines. We compared the effects of explant collection time, family and medium components (concentrations of sucrose, plant growth regulators and acid-hydrolyzed casein) on embryogenic lines induction. The effects of plant growth regulators and L-glutamine contents on the proliferation and maturation of embryogenic cell lines were studied, and the germinating ability of different cell lines was evaluated. The embryogenic lines induction percentage of Korean pine reached 33.33%. When 4.52 ?mol·L-1 2,4-D and 2.2 ?mol·L-1 6-BA were added to the medium of embryogenic lines proliferation, the ability of embryo maturation was the best (cell line 001#-100 was 135.71·g-1 fresh weight). Adding 1-1.5g L-1 L-glutamine to the proliferation medium can improve the ability of embryo maturation (cell line 001#-100 was 165.63·g-1 fresh weight). The germination percentage of the three cell lines tested was significant, and the highest was 66%. We report on successful regeneration and cryopreservation methods for somatic embryos of Korean pine. This technology could be used to propagate the excellent germplasm resources of Korean pine and to establish multi-varietal forestry. © 2020 by the authors.

Scopus

Держатели документа:
State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China
Laboratory of Forest Genetics and Breeding, Institution of the Russian Academy of Sciences V.N., Sukachev Institute of Forest Siberian Branch of RAS, Krasnoyarsk, 660036, Russian Federation
Department of Cell Biology and Institute of Plant Physiology K.A., Timiryazev Russian Academy of Sciences, Moscow, 127276, Russian Federation
Department of Plant Physiology, Biological Faculty, Lomonosov Moscow State University, Moscow, 119991, Russian Federation
State Forestry and Grassland Administration Engineering Technology Research Center of Korean Pine, Harbin, 150040, China

Доп.точки доступа:
Gao, F.; Peng, C.; Wang, H.; Nikolaevna, I.; Mikhaylovich, A.; Shen, H.; Yang, L.

/ F. Gao, C. X. Peng, H. Wang [et al.] // Forests. - 2020. - Vol. 11, Is. 9. - Ст. 912, DOI 10.3390/f11090912. - Cited References:37. - The work was supported by the National Key R&D Program of China (2017YFD0600600), and the Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University, 2016C01). . - ISSN 1999-4907РУБ Forestry

Аннотация: Korean pine is the dominant species of Korean pine forests. It is an economically valuable species that yields oil, high-quality timber and nuts, and it offers great prospects for further development. Complete regenerated plants of Korean pine were obtained via somatic embryogenesis using megagametophytes as the explant. The seeds of 27 families of Korean pine were collected to induce embryogenic lines. We compared the effects of explant collection time, family and medium components (concentrations of sucrose, plant growth regulators and acid-hydrolyzed casein) on embryogenic lines induction. The effects of plant growth regulators and L-glutamine contents on the proliferation and maturation of embryogenic cell lines were studied, and the germinating ability of different cell lines was evaluated. The embryogenic lines induction percentage of Korean pine reached 33.33%. When 4.52 mu mol center dot L(-1)2,4-D and 2.2 mu mol center dot L(-1)6-BA were added to the medium of embryogenic lines proliferation, the ability of embryo maturation was the best (cell line 001#-100 was 135.71 center dot g(-1)fresh weight). Adding 1-1.5g L-1L-glutamine to the proliferation medium can improve the ability of embryo maturation (cell line 001#-100 was 165.63 center dot g(-1)fresh weight). The germination percentage of the three cell lines tested was significant, and the highest was 66%. We report on successful regeneration and cryopreservation methods for somatic embryos of Korean pine. This technology could be used to propagate the excellent germplasm resources of Korean pine and to establish multi-varietal forestry.

WOS

Держатели документа:
Northeast Forestry Univ, Sch Forestry, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China.
RAS, Forest Siberian Branch, Sukachev Inst, Lab Forest Genet & Breeding,Inst Russian Acad Sci, Krasnoyarsk 660036, Russia.
Timiryazev Russian Acad Sci, Dept Cell Biol, Moscow 127276, Russia.
Timiryazev Russian Acad Sci, Inst Plant Physiol KA, Moscow 127276, Russia.
Lomonosov Moscow State Univ, Fac Biol, Dept Plant Physiol, Moscow 119991, Russia.
State Forestry & Grassland Adm Engn Technol Res C, Harbin 150040, Peoples R China.

Доп.точки доступа:
Gao, Fang; Peng, Chunxue; Wang, Hao; Tretyakova, Iraida Nikolaevna; Nosov, Alexander Mikhaylovich; Shen, Hailong; Yang, Ling; National Key R&D Program of China [2017YFD0600600]; Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) [2016C01]

[Текст] : статья / О. Э. Пашкеева, И. Д. Гродницкая, Г. И. Антонов [и др.] // Лесоведение. - 2021. - № 2. - С. 143-155 . - ISSN 0024-1148

УДК	632.93+579.264

Аннотация: Исследования проводили на территории опытного питомника экспериментально-опытного хозяйства “Погорельский бор” Института леса им. В.Н. Сукачева СО РАН. В модельных полевых экспериментах изучали влияние предпосевной обработки семян сосны обыкновенной (<i>Pinus sylvestris</i> L.) водными суспензиями аборигенных микроорганизмов, обладающих антагонистической активностью к фитопатогенам, и фитопрепаратами, полученными из растительного сырья, на микробную биомассу, ферментативную активность почвы и сохранность сеянцев хвойных. Обработку семян сосны проводили штаммами микромицетов <i>Trichoderma harzianum</i>, <i>T. longibrachiatum</i>, <i>T. lignorum</i>, их смесью; бактериями <i>Bacillus amyloliquefaciens</i>, смесью бактерий (<i>B. amyloliquefaciens</i>, <i>B.</i> <i>subtilis</i>, <i>Pseudomonas</i> sp.) и фитопрепаратами (пять вариантов). Показано, что предпосевная обработка семян сосны способствовала улучшению их всхожести, сохранности сеянцев к концу сезона вегетации, морфометрических параметров сеянцев по сравнению с контролем в среднем на 25%. Интродуцированные с семенами популяции бактерий и микромицетов повышали продуктивность, содержание микробной биомассы и ферментативную активность почвы лесного питомника в 1.3–2.0 раза. Установлено, что в контрольной почве на протяжении всего периода вегетации преобладала олиготрофная группа микроорганизмов, а при обработке семян сосны (микроорганизмы и фитопрепараты) уже через два месяца после посева семян доминировала гидролитико-копиотрофная группа. Внесение микробов-антагонистов (<i>B. amyloliquefaciens</i>, <i>T. longibrachiatum</i>, Смесь бактерий) и фитопрепаратов также благоприятно влияло на функционирование почвенного микробного сообщества, что проиллюстрировано уменьшением значений микробного метаболического коэффициента в среднем почти в 2 раза по сравнению с контролем. Результаты исследования можно рекомендовать для использования в практике лесного хозяйства при выращивании сеянцев хвойных.
The studies were carried out on the experimental farm “Pogorelsky bor” of the Sukachеv Institute of Forest, SB RAS. The effect of pre-sowing treatment of Scots pine seeds (<i>Pinus sylvestris</i> L.) by aqueous suspensions of indigenous microorganisms with antagonistic activity towards phytopathogens and phytopreparations obtained from plant materials on soil biogenicity (microbial biomass and enzymatic activity) and safety of coniferous seedlings was studied in model field experiments. Strains of micromycetes <i>Trichoderma harzianum</i>, <i>Trichoderma longibrachiatum</i>, <i>Trichoderma lignorum</i> and their mixture (<i>T. harzianum, T. longibrachiatum, T. lignorum</i>); Bacteria <i>Bacillus amyloliquefaciens</i>, their mixture (<i>B. amyloliquefaciens</i>, <i>B. subtilis</i>, <i>Pseudomonas</i> sp.), five phytopreparations were variants of Scots pine seed treatments. It was shown that the pre-sowing treatment of Scots pine seeds contributed to the improvement of their germination, preservation of seedlings by the end of the vegetation season, and improved morphometric parameters of the seedlings compared with the control by 24.5% on the average. The bacteria and micromycetes introduced with the seeds increased the productivity and biogenicity of the forest nursery soil by 1.3–2.0 times. It was established that the oligotrophic group of microorganisms prevailed in the control soil throughout the vegetation season, while the hydrolytic-copyotrophic group of microorganisms prevailed after the treatment of Scots pine seeds with microorganisms and phytopreparations two months after sowing seeds. The introduction of antagonist microbes (<i>B.amyloliquefaciens</i>, <i>T. longibrachiatum</i>, and a mixture of bacteria) and phytopreparations also favorably affected the restoration of the ecophysiological norm of functioning of the soil microbial community (returning the value of the microbial metabolic coefficient to the background ones). A 2-fold decrease in the values of the microbial metabolic coefficient (<i>q</i>CO₂) was observed in the soil of the studied areas. The obtained results can be recommended for using in forestry practice when growing coniferous seedlings.

РИНЦ

Держатели документа:
Институт леса им. В.Н. Сукачева СО РАН
Институт химии твердого тела и механохимии СО РАН
Сибирский федеральный университет

Доп.точки доступа:
Пашкеева, О.Э.; Гродницкая, И.Д.; Антонов, Г.И.; Ломовский, О.И.; Гайдашева, И.И.

/ Y. A. Litovka, P. V. Makolova, A. A. Leonenko [et al.] // IOP Conference Series: Earth and Environmental Science : IOP Publishing Ltd, 2021. - Vol. 848: 5th International Workshop on Innovations in Agro and Food Technologies, WIAFT-V 2021 (17 June 2021 through 18 June 2021, ) Conference code: 172027, Is. 1. - Ст. 012163, DOI 10.1088/1755-1315/848/1/012163 . -
Аннотация: A phytopathogenic complex of the genus Fusarium, common on 19 wheat varieties cultivated in Siberia, was investigated. It was found eleven fungal species in wheat grain (Fusarium avenaceum, F. culmorum, F. diversisporum, F. heterosporum, F. oxysporum, F.poae, F. sambucinum, F. incarnatum, F. sporotrichioides, F. tricinctum, Neocosmospora solani), with the dominant one being F. sporotrichioides (from 23 to 68 % of the total number of species). Seven species were found in the rhizosphere of wheat (F. avenaceum, F.incarnatum, F. oxysporum, F. sambucinum, F. sporotrichioides, F. tricinctum, N. solani), with the most common being F. oxysporum (up to 39 %) and F. sporotrichioides (up to 35%). Biotesting a mixture of metabolites in wheat seeds and seedlings revealed highly toxic species, namely F. sporotrichioides and F. oxysporum (a decrease of in vitro seed germination by more than 55 % and a prolonged inhibitory effect on seedling development). F. avenaceum, N.solani and F. culmorum are moderately toxic species. The greatest danger for the development of wheat fusariosis in Siberia in terms of the total frequency of occurrence, phytotoxic and phytopathogenic properties is represented by F. sporotrichioides. © Published under licence by IOP Publishing Ltd.

Scopus

Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Reshetnev Siberian State University of Science and Technology, 31, Krasnoyarsky rabochy prospect, Krasnoyarsk, 660037, Russian Federation

Доп.точки доступа:
Litovka, Y. A.; Makolova, P. V.; Leonenko, A. A.; Vasilieva, A. A.; Kokorin, A. N.; Pavlov, I. N.

[Текст] / В. А. Сенашова, А. А. Анискина, Г. Г. Полякова // Сибирский экологический журнал. - 2023. - Т. 30, № 4. - С. 446-458, DOI 10.15372/SEJ20230404 . - ISSN 0869-8619

ГРНТИ

68.47

68.47.03

Аннотация: Рассмотрено взаимодействие в системе "эпифитные микроорганизмы - растение-хозяин - патоген" на примере сеянцев сосны обыкновенной (Pinus sylvestris L.), искусственно зараженных факультативным сапротрофом Lophodermium seditiosum Minter, Staley & Millar. При этом исследовали изменение численности эпифитных микроорганизмов, состава и концентрации летучих соединений, морфологических параметров сосны в разных вариантах опыта. Рассматривали влияние на исследуемые характеристики двух факторов: заражение L. seditiosum и внесение микокомпоста в почву. Использовали стандартные фитопатологические и микробиологические методы. Летучие соединения изучали с помощью хромато-масс-спектрометрии, фитонцидную активность - адаптированным методом Токина. На стадии прорастания аскоспоры отмечено увеличение количества летучих компонентов, выделяемых пораженной хвоей, и уменьшение числа таковых, выделяемых корнями зараженных растений. При этом содержание α-пинена в летучей фракции хвоинок с некротическими пятнами снижено на 24 % по сравнению со здоровыми. В опытных образцах доли некоторых терпенов увеличиваются, так, содержание 3-карена превысило в 2,3 раза контрольные значения. На фоне изменения компонентного состава летучих соединений установлено повышение фитонцидной активности листовой и корневой систем растений с признаками заболевания по сравнению с экземплярами без признаков повреждений. Об этом же свидетельствуют и результаты микробиологических посевов: в вариантах с заражением по сравнению с контрольными отмечено достоверное снижение численности эпифитных микроорганизмов. Установлено достоверное увеличение массы хвоинок в вариантах с инфицированием и при внесении микокомпоста, подтвержденное анализом ANOVA. Зарегистрированные эффекты соответствуют адаптационному синдрому Селье (активации физиологических процессов на раннем этапе неблагоприятного воздействия, сменяющейся истощением организма, если нагрузка продолжается)
The interaction in the system "epiphytic microorganisms - host plant-pathogen" was considered on the example of Scotch pine (Pinus sylvestris L.) seedlings which artificially infected with the facultative saprotroph Lophodermium seditiosum Minter, Staley & Millar. At the same time, the change of quantity of the epiphytic microorganisms, the composition and concentration of volatile compounds and the morphological parameters of pine in different variants of the experiment were studied. The influence of two factors on the studied characteristics was considered: L. seditiosum infection and the introduction of mycocompost into the soil. Classical phytopathological and microbiological methods were used. Volatile compounds were studied using chromato-mass spectrometry, phytoncide activity was studied using the Tokin method adapted to our conditions. At the stage of ascospore germination, an increase in the amount of volatile components released by the affected needles and a decrease in the number of those released by the roots of infected plants were noted. Against the background of changes in the composition of volatile compounds, an increase in the phytoncide activity of the leaf and root systems of plants with signs of the disease was found compared to specimens without signs of damage. This is also evidenced by the results of microbiological cultures: in the variants with infection, compared with the control, a significant decrease in the number of epiphytic microorganisms was noted. A significant increase in the mass of needles was noted in the variants with infection and with the adding of mycocompost, confirmed by ANOVA analysis. Registered correspond to Selye’s adaptive syndrome (activation of physiological processes at an early stage of adverse effects, followed by exhaustion of the body if the load continues)

РИНЦ

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Анискина, Антонина Александровна; Aniskina Antonina Alexandrovna; Полякова, Галина Геннадьевна; Polyakova Galina Gennad'yevna; Syenashova Vera Alexandrovna

[Текст] / И. Д. Гродницкая, В. А. Сенашова, Г. И. Антонов, Г. Г. Полякова [и др.] // Почвоведение. - 2023. - № 9. - С. 1173-1189, DOI 10.31857/S0032180X23600415 . - ISSN 0032-180X
Аннотация: Биологическую активность темно-серой почвы исследовали в 100-летнем сосняке разнотравно-зеленомошном Погорельского бора Красноярской лесостепи. В 2017 г. в сосняке были проведены выборочные рубки, а в мае 2022 г. произошел сильный пожар. Для улучшения лесовосстановления и повышения биологической продуктивности почвы на вырубленные и горевшие участки вносили биоудобрение на основе опилочно-почвенного субстата с микопродуктом и мочевиной (ОПСМ + М). На экспериментальных участках (пасека, волок, фон) проводили ежегодный учет самосева сосны. Биоиндикацию состояния почвы оценивали на основании общей численности и соотношения долей эколого-трофических групп микроорганизмов, активности ферментов, содержания микробной биомассы, интенсивности базального дыхания и удельного дыхания микробной биомассы. Внесение биоудобрения на вырубленные участки способствовало подщелачиванию почвы на 0.2–0.4 ед., сохранению влажности, увеличению содержания азота (на 5–14%) и микробной биомассы (в 1.2–1.6 раза) по сравнению с контрольными вариантами. Воздействие биоудобрений на всхожесть и рост самосева сосны отмечено на второй год после внесения: на опытных участках самосева было в 4–6 раз больше, чем на контрольных. Поступление в почву обгоревших растительных остатков, углей и золы в первую неделю после пожара привело к активизации микроорганизмов-карботрофов, которые увеличивали общую численность микроорганизмов, микробную биомассу, активность уреазы и инвертазы. Однако к концу вегетационного периода отмечали снижение микробиологической активности, что указывало на постпирогенную депрессию микробоценозов. Внесение биоудобрения на сгоревшую поверхность участков нивелировало влияние пирогенного воздействия и стимулировало образование всходов сосны обыкновенной, количество которых было достоверно больше, чем на контрольных участках. Установлено, что универсальными биоиндикаторами, адекватно отражающими состояние почвы после всех антропогенных воздействий, были микробная биомасса, удельное микробное дыхание, ферментативная активность и общая численность микроорганизмов. Специфической биоиндикацией состояния почвы после пожара являлось увеличение доли бактерий Serratia plymuthica, Bacillus mycoides и грибов родов Trichoderma, Penicillium и Mortierela.
3The biological activity of dark gray soil was studied in a 100-year-old pine forb-green-moss forest of the Pogorelsky pine forest of the Krasnoyarsk forest-steppe. In 2017, selective cuttings were carried out in the pine forest, and in May 2022 there was a strong fire. To improve reforestation and increase the biological productivity of the soil, bio-fertilizer based on sawdust-soil substrate with the addition of urea and mycoproduct (SSSU + M) was applied to cut and burned areas. On the experimental plots (Paseka, Volok, Fon), an annual count of self-seeding of pine was carried out. Bioindication of the soil condition was assessed based on the total number and ratio of ecological-trophic groups of microorganisms, enzyme activity, microbial biomass content, intensity of basal respiration and specific respiration of microbial biomass. The application of biofertilizer to the cut areas alkalized the soil by 0.2–0.4 units, retained moisture, increased the content of nitrogen (by 5–14%) and microbial biomass (by 1.2–1.6 times), compared with the control options. The impact of biofertilizers on the germination and growth of self-seeding of pine was noted in the second year after application – in the experimental plots of self-seeding it was 4–6 times greater than in the control ones. The entry of burnt plant residues, coals and ash into the soil in the first week after the fire led to an increase in some microbiological indicators, the activity of urease and invertase, and the activation of carbotrophic microorganisms. However, by the end of the growing season, a decrease in microbiological activity was noted, which indicated a post-pyrogenic depression of microbocenoses. The introduction of biofertilizer on the burnt surface of the plots leveled the effect of pyrogenic effects and stimulated the formation of shoots of scots pine, the number of which was significantly higher than in the control plots. It was found that the universal bioindicators that adequately reflect the state of the soil after all anthropogenic impacts were microbial biomass, specific microbial respiration, enzymatic activity and the total number of microorganisms. A specific bioindication of the soil condition after the fire was an increase in the proportion of bacteria Serratia plymuthica, Bacillus mycoides and fungi of the genera Trichoderma, Penicillium and Mortierela

РИНЦ

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Сенашова, Вера Александровна; Syenashova Vera Alexandrovna; Антонов, Георгий Иванович; Antonov Georgy Ivanovich; Полякова, Галина Геннадьевна; Polyakova Galina Gennad'yevna; Пашкеева, Оксана Эриковна; Пашенова, Наталья Вениаминовна; Pashenova, Natal'ya Veniaminovna; Grodnitskaya, Irina Dmitriyevna

[Текст] : научное издание / Е. В. Бажина, Л. Н. Скрипальщикова, А. С. Шушпанов // Сибирский экологический журнал. - 2023. - Т. 30, № 5. - С. 626-634, DOI 10.15372/SEJ20230505 . - ISSN 0869-8619

ГРНТИ

68.47.03

Аннотация: Береза повислая (Betula pendula Roth.) характеризуется как устойчивый к техногенному загрязнению среды вид древесных растений. Известно, что генеративные процессы растений и, в частности, формирование и жизнеспособность пыльцы крайне чувствительны к воздействию техногенного загрязнения. Исследования жизнеспособности пыльцы проводились в условиях Средней Сибири, в наиболее распространенной группе типов леса - березняках разнотравных, произрастающих в пригородной зоне крупного промышленного центра г. Красноярска, на восьми пробных площадях. Несмотря на устойчивость вида, мужские генеративные органы B. pendula оказались чувствительными к воздействию промышленного загрязнения. Исследования показали снижение качества пыльцы при усилении степени техногенной нагрузки, достоверных различий по диаметру пыльцевых зерен в различных условиях произрастания, как и деформированных пыльцевых зерен не обнаружено. Уровень изменчивости размеров пыльцевых зерен по диаметру в биоценозе не превышал 9,3-9,9 %. Максимальное число проросших пыльцевых зерен отмечалось в фоновых условиях, минимальное - на восточной окраине города, по основному направлению переноса техногенных аэрозолей. Длина пыльцевых трубок у деревьев, растущих в этих условиях, уменьшалась более чем в 2 раза по отношению к критическому значению (диаметр пыльцевого зерна 18-20 мкм). Выявлены взаимосвязи прорастания пыльцы и количества пыли, аккумулированной на листовых пластинках, а также содержания в ассимиляционной массе свинца и алюминия ( r = - 0,1-0,3; р ≤ 0,05). Функциональные показатели пыльцы березы повислой можно рекомендовать в качестве биоиндикационного показателя при проведении мониторинга окружающей среды.
Betula pendula Roth. is tolerant to tehchnogenic air pollutants. However, the viability of pollen decreases under stress conditions, as well as under air pollution. The researches were conducted in Middle Siberia on eight plots of motley birches near industrial city of Krasnoyarsk. In each plot on west side of 15-20 trees, male twigs were cut at pollination time. Pollen was extracted from the male generative organs for size and viability (potential, by starch test, and realized, by in vitro germination) determination. The results were analyzed by statistical methods. The researches have revealed reduction of pollen viability under technogenic pollution and reliable differences in pollen grain diameters under different growing conditions, whereas deformed pollen grain were not revealed. The variability of pollen grains in diameter in the biocenosis did not exceed 9.3-9.9 %. Maximum number of sprouted pollen grains observed under background conditions, and minimum at the eastern border of city in the main direction of the transfer of technogenic aerosols. The length of pollen tubes in trees growing under these conditions decreased by more than twice in comparison to the critical value (namely, pollen grain diameter of 18-20 µm). Regression analysis has shown correlations between pollen germination and accumulation of dust on foliage, as well as of lead and aluminum content in the foliage ( r = - 0.1-0.3, р ≥ 0.05)). In spite of tolerance of the species to air pollution, Betula pendula male generative structures are sensitive to the contamination. Pollen viability can be used as a bioindicator of environmental pollution at monitoring.

РИНЦ

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Скрипальщикова, Лариса Николаевна; Skripalshchikova, Larisa Nikolayevna; Шушпанов, Александр Сергеевич; SHUSHPANOV A.S.; Bazhina, Elena Vasil'yevna