Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ M. I. Makarov [et al.] // Eurasian Soil Sci. - 2013. - Vol. 46, Is. 7. - P778-787, DOI 10.1134/S1064229313070053. - Cited References: 32. - This study was supported by the Russian Foundation for Basic Research (project no. 10-04-00780). . - 10. - ISSN 1064-2293РУБ Soil Science

Аннотация: The drying of samples of mountain-meadow soils characterized by their permanently high moisture under natural conditions fundamentally changes the concentrations of the labile nitrogen and carbon compounds, as well as the patterns of their microbial transformation. When the soil samples are dried, a four- to fivefold increase in the content of the extractable organic nitrogen compounds, carbon compounds, and inorganic nitrogen compounds is observed, while the content of nitrogen and carbon of the microbial biomass decreases by two-three times. The rewetting of the dried soil launches the process of the replenishment of the nitrogen and carbon reserves in the microbial biomass. However, even after two weeks of incubation, their values were 1.5-2 times lower than the initial values typical of the natural soil. The restoration of the microbial community in the samples of the previously dried soils occurs in the absence of a deficiency of labile organic compounds and is accompanied by their active mineralization and the low uptake of ammonium nitrogen by the microorganisms.

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Makarov, M. I.
Mulyukova, O. S.
Malysheva, T. I.] Moscow MV Lomonosov State Univ, Fac Soil Sci, Moscow 119992, Russia
[Menyailo, O. V.] Russian Acad Sci, Siberian Branch, Sukachev Inst Forestry, Krasnoyarsk, Russia

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Makarov, M.I.; Mulyukova, O.S.; Malysheva, T.I.; Menyailo, O.V.

[Text] / O. V. Menyailo [et al.] // Glob. Change Biol. - 2008. - Vol. 14, Is. 10. - P2405-2419, DOI 10.1111/j.1365-2486.2008.01648.x. - Cited References: 62. - We thank Esther Surges for the isotope ratio measurements, V. Menyailo and V. Novikov for the help with field flux measurements, A. Pimenov for botanical description of the grassland and P. Frenzel for discussion of the data. We are deeply grateful to the staff of Soil Science Department of the Institute of Forest in Krasnoyarsk for creation and maintaining the afforestation experiment over the last 35 years. The work was funded by the US Civilian Research and Development Foundation (USA) and by the Alexander von Humboldt Foundation (Germany). . - 15. - ISSN 1354-1013РУБ Biodiversity Conservation + Ecology + Environmental Sciences

Аннотация: Forest ecosystems assimilate more CO(2) from the atmosphere and store more carbon in woody biomass than most nonforest ecosystems, indicating strong potential for afforestation to serve as a carbon management tool. However, converting grasslands to forests could affect ecosystem-atmosphere exchanges of other greenhouse gases, such as nitrous oxide and methane (CH(4)), effects that are rarely considered. Here, we show that afforestation on a well-aerated grassland in Siberia reduces soil CH(4) uptake by a factor of 3 after 35 years of tree growth. The decline in CH(4) oxidation was observed both in the field and in laboratory incubation studies under controlled environmental conditions, suggesting that not only physical but also biological factors are responsible for the observed effect. Using incubation experiments with (13)CH(4) and tracking (13)C incorporation into bacterial phospholipid fatty acid (PLFA), we found that, at low CH(4) concentrations, most of the (13)C was incorporated into only two PLFAs, 18 : 1 omega 7 and 16 : 0. High CH(4) concentration increased total (13)C incorporation and the number of PLFA peaks that became labeled, suggesting that the microbial assemblage oxidizing CH(4) shifts with ambient CH(4) concentration. Forests and grasslands exhibited similar labeling profiles for the high-affinity methanotrophs, suggesting that largely the same general groups of methanotrophs were active in both ecosystems. Both PLFA concentration and labeling patterns indicate a threefold decline in the biomass of active methanotrophs due to afforestation, but little change in the methanotroph community. Because the grassland consumed CH(4) at a rate five times higher than forest soils under laboratory conditions, we concluded that not only biomass but also cell-specific activity was higher in grassland than in afforested plots. While the decline in biomass of active methanotrophs can be explained by site preparation (plowing), inorganic N (especially NH(4)(+)) could be responsible for the change in cell-specific activity. Overall, the negative effect of afforestation of upland grassland on soil CH(4) uptake can be largely explained by the reduction in biomass and to a lesser extent by reduced cell-specific activity of CH(4)-oxidizing bacteria.

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[Menyailo, Oleg V.] Inst Forest SB RAS, Krasnoyarsk 660036, Russia
[Menyailo, Oleg V.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Menyailo, Oleg V.
Conrad, Ralf] Max Planck Inst Terr Microbiol, D-35043 Marburg, Germany
[Hungate, Bruce A.] No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86001 USA
[Hungate, Bruce A.] No Arizona Univ, Merriam Powell Ctr Environm Res, Flagstaff, AZ 86001 USA
[Abraham, Wolf-Rainer] Helmholtz Ctr Infect Res, D-38124 Braunschweig, Germany

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Menyailo, O.V.; Hungate, B.A.; Abraham, W.R.; Conrad, R...

/ N. -N. Zhao [et al.] // Plant Soil. - 2014. - Vol. 384, Is. 1-2. - P289-301, DOI 10.1007/s11104-014-2210-x . - ISSN 0032-079X
Аннотация: Background Meadows and shrublands are two major vegetation types on the Qinghai-Tibetan Plateau, but little is known about biochemical characteristics and its relation to decomposability of soil organic carbon (OC) under these two vegetation types. The present study was designed to evaluate effects of aspect-vegetation complex on biochemical characteristics and decomposability of soil OC. Methods Two hills were randomly selected; both with vegetation being naturally divided into southward meadows and northward shrublands by a ridge, and soils were sampled at depths of 0-15 and 15-30 cm, along contours traversing the meadow and shrubland sites. Particulate (particle size 2-0.05 mm) OC and nitrogen (N), microbial biomass C and N, non-cellulosic sugars, and CuO lignin were analyzed, and OC mineralization was measured for 49 days at 18 and 25 °C under laboratory incubation, respectively. Results More than half of soil OC was present as particulate fraction across all samples, indicating the coarse nature of soil organic matter in the region. Averaging over depths, shrublands contained 87.7 - 114.1 g OC and 7.7 - 9.3 g N per kg soil, which were 63 - 78 and 26 - 31 % higher than those in meadows, respectively. Meanwhile the C/N ratio of soil organic matter was 11.4 - 12.3 under shrublands, being 29 - 40 % higher than that under meadows. Soil OC under meadows was richer in noncellulosic carbohydrates and microbial biomass in the 0-15 and 15-30 cm depths but contained less lignin in the 15-30 cm depth. Ratios of microbially- to plant-derived monosaccharides and between acid and aldehyde of the vanillyl units were greater in soils under shrublands, showing more abundant microbially-derived sugars and microbially-transformed ligneous substances in OC as compared to meadow soils. By the end of 49 days' incubation, total CO2-C evolution from soils under meadows was 15.0-16.2 mg g-1 OC averaging over incubation temperatures and soil depths, being 27-55 % greater than that under shrublands. Across all soil samples over two sites, total CO2 - C evolved per g OC at either 18 or 25 °C was closely correlated to enrichments of noncellulosic carbohydrates and microbial biomass. This indicates that the greater soil OC decomposability under meadows was associated with its larger abundances of readily mineralizable fractions compared with shrublands. However, temperature increase effect on soil OC decomposability did not differ between the two types of vegetation. Conclusions Our findings suggest that the aspect-vegetation complex significantly affected pool size, biochemical characteristics, and decomposability of soil OC on the northeastern edge of Qinghai-Tibetan Plateau. However, the response of soil OC decomposability to temperature was similar between southward meadows and northward shrublands. © 2014 Springer International Publishing Switzerland.

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Держатели документа:
School of Life Sciences, Lanzhou University, Lanzhou, 730000, China
Institute of Soil Science, Leibniz Universitat Hannover, Hannover, 30419, Germany
VN Sukachev Institute of Forest, Krasnoyarsk, 660036, Russian Federation
King Saud University, Riyadh, Saudi Arabia

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Zhao, N.-N.; Guggenberger, G.; Shibistova, O.; Thao, D.T.; Shi, W.J.; Li, X.G.

/ M. I. Makarov [et al.] // Russ. J. Ecol. - 2015. - Vol. 46, Is. 4. - P317-324, DOI 10.1134/S1067413615040116 . - ISSN 1067-4136
Аннотация: Freezing-thawing of alpine meadow soils results in a 1.5- to 2-fold increase in the contents of extractable organic and inorganic nitrogen and organic carbon compounds, whereas the contents of microbial biomass nitrogen and carbon slightly decrease. The latter are quickly restored in the course of subsequent incubation, but the processes of transformation of nitrogen compounds proceed differently in soils that are subject to freezing under natural conditions and in nonfreezing soils. In nonfreezing soil, an abrupt activation of organic nitrogen mineralization and nitrification takes place against the background of a relatively low level of microbial assimilation of inorganic nitrogen compounds by microorganisms. © 2015, Pleiades Publishing, Ltd.

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Держатели документа:
Moscow State University, Moscow, Russian Federation
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation

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Makarov, M.I.; Malysheva, T.I.; Mulyukova, O.S.; Menyailo, O.V.

/ P. Capek [et al.] // Soil Biol. Biochem. - 2015. - Vol. 90. - P19-29, DOI 10.1016/j.soilbio.2015.07.013 . - ISSN 0038-0717
Аннотация: Arctic permafrost soils contain large stocks of organic carbon (OC). Extensive cryogenic processes in these soils cause subduction of a significant part of OC-rich topsoil down into mineral soil through the process of cryoturbation. Currently, one-fourth of total permafrost OC is stored in subducted organic horizons. Predicted climate change is believed to reduce the amount of OC in permafrost soils as rising temperatures will increase decomposition of OC by soil microorganisms. To estimate the sensitivity of OC decomposition to soil temperature and oxygen levels we performed a 4-month incubation experiment in which we manipulated temperature (4-20 °C) and oxygen level of topsoil organic, subducted organic and mineral soil horizons. Carbon loss (CLOSS) was monitored and its potential biotic and abiotic drivers, including concentrations of available nutrients, microbial activity, biomass and stoichiometry, and extracellular oxidative and hydrolytic enzyme pools, were measured. We found that independently of the incubation temperature, CLOSS from subducted organic and mineral soil horizons was one to two orders of magnitude lower than in the organic topsoil horizon, both under aerobic and anaerobic conditions. This corresponds to the microbial biomass being lower by one to two orders of magnitude. We argue that enzymatic degradation of autochthonous subducted OC does not provide sufficient amounts of carbon and nutrients to sustain greater microbial biomass. The resident microbial biomass relies on allochthonous fluxes of nutrients, enzymes and carbon from the OC-rich topsoil. This results in a "negative priming effect", which protects autochthonous subducted OC from decomposition at present. The vulnerability of subducted organic carbon in cryoturbated arctic soils under future climate conditions will largely depend on the amount of allochthonous carbon and nutrient fluxes from the topsoil. © 2015 Elsevier Ltd.

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University of South Bohemia, Department of Ecosystems Biology, Branisovska 31, Ceske Budejovice, Czech Republic
Institute of Systematic Botany and Ecology, University of Ulm, Albert-Einstein-Allee 11, Ulm, Germany
University of Vienna, Department of Microbiology and Ecosystem Research, Division of Terrestrial Ecosystem Research, Althanstrasse 14, Vienna, Austria
Austrian Polar Research Institute, Althanstrasse 14, Vienna, Austria
University of Gothenburg, Department of Earth Sciences, Guldhedsgatan 5A, Gothenburg, Sweden
University of New Hampshire, Department of Natural Resources and the Environment, Durham, NH, United States
University of Vienna, Department of Ecogenomics and Systems Biology, Division of Archaea Biology and Ecogenomics, Vienna, Austria
Leibniz Universitat Hannover, Institute of Soil Science, Herrenhauser Strasse 2, Hannover, Germany
Martin-Luther-University Halle-Wittenberg, Soil Sciences, Halle, Germany
University of Stockholm, Department of Physical Geography, Stockholm, Sweden
Central Siberian Botanical Garden, Siberian Branch of the Russian Academy of Sciences, St. Zolotodolinskaya 101, Novosibirsk, Russian Federation
University of Bergen, Department of Biology, Centre for Geobiology, Thormohlensgate 53B, Bergen, Norway
Center for Geomicrobiology, Department of Bioscience, Ny Munkegade 114, Aarhus C, Denmark
VN Sukachev, Institute of Forest, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
University of Greifswald, Institute for Microbiology, Greifswald, Germany

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Capek, P.; Diakova, K.; Dickopp, J.-E.; Barta, J.; Wild, B.; Schnecker, J.; Alves, R.J.E.; Aiglsdorfer, S.; Guggenberger, G.; Gentsch, N.; Hugelius, G.; Lashchinsky, N.; Gittel, A.; Schleper, C.; Mikutta, R.; Palmtag, J.; Shibistova, O.; Urich, .; Richter, A.; Santruckova, H.

/ Z. Zhu [et al.] // Biogeosciences. - 2016. - Vol. 13, Is. 15. - P4481-4489, DOI 10.5194/bg-13-4481-2016 . - ISSN 1726-4170
Аннотация: The input of recently photosynthesized C has significant implications on soil organic C sequestration, and in paddy soils, both plants and soil microbes contribute to the overall C input. In the present study, we investigated the fate and priming effect of organic C from different sources by conducting a 300-day incubation study with four different 13C-labelled substrates: rice shoots (shoot-C), rice roots (root-C), rice rhizodeposits (rhizo-C), and microbe-assimilated C (micro-C). The efflux of both 13CO2 and 13CH4 indicated that the mineralization of C in shoot-C-, root-C-, rhizo-C-, and micro-C-treated soils rapidly increased at the beginning of the incubation and decreased gradually afterwards. The highest cumulative C mineralization was observed in root-C-treated soil (45.4%), followed by shoot-C- (31.9%), rhizo-C- (7.90%), and micro-C-treated (7.70%) soils, which corresponded with mean residence times of 39.5, 50.3, 66.2, and 195 days, respectively. Shoot and root addition increased C emission from native soil organic carbon (SOC), up to 11.4 and 2.3 times higher than that of the control soil by day 20, and decreased thereafter. Throughout the incubation period, the priming effect of shoot-C on CO2 and CH4 emission was strongly positive; however, root-C did not exhibit a significant positive priming effect. Although the total C contents of rhizo-C-(1.89%) and micro-C-treated soils (1.90%) were higher than those of untreated soil (1.81%), no significant differences in cumulative C emissions were observed. Given that about 0.3 and 0.1% of the cumulative C emission were derived from labelled rhizo-C and micro-C, we concluded that the soil organic C-derived emissions were lower in rhizo-C- and micro-C-treated soils than in untreated soil. This indicates that rhizodeposits and microbe-assimilated C could be used to reduce the mineralization of native SOC and to effectively improve soil C sequestration. The contrasting behaviour of the different photosynthesized C substrates suggests that recycling rice roots in paddies is more beneficial than recycling shoots and demonstrates the importance of increasing rhizodeposits and microbe-assimilated C in paddy soils via nutrient management. © 2016 Author(s).

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Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, China
Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, China
Institute of Soil Science, Leibniz Universitat Hannover, Hannover, Germany
VN Sukachev Institute of Forest, Siberian Branch, Russian Academy of Science, Krasnoyarsk, Russian Federation
College of Resources and Environment, Southwest University, Chongqing, China

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Zhu, Z.; Zeng, G.; Ge, T.; Hu, Y.; Tong, C.; Shibistova, O.; He, X.; Wang, J.; Guggenberger, G.; Wu, J.

/ O. V. Masyagina, I. V. Tokareva, A. S. Prokushkin // Sci. Total Environ. - 2016. - Vol. 573. - P1255-1264, DOI 10.1016/j.scitotenv.2016.04.195. - Cited References:52. - The reported study was partially supported by the Russian Science Foundation (14-24-00113). . - ISSN 0048-9697. - ISSN 1879-1026РУБ Environmental Sciences

Аннотация: Periodical ground fires of high frequency in permafrost forest ecosystems of Siberia (Russian Federation) are essential factors determining quantitative and qualitative parameters of permafrost soil organic matter. Specific changes in physical and chemical parameters and microbial activity of permafrost soil mineral horizons of northern taiga larch stands were revealed after heating at high temperatures (150-500 degrees C) used for imitation of different burn intensities. Burning at 150-200 degrees C resulted in decreasing of soil pH, whilst heating at 300-500 degrees C caused increase of pH compare to unheated soils. Water-soluble organic carbon concentration in permafrost soils heated at 150-200 degrees C was much higher than that of unheated soils. All these changes determined soil microbial activity in heated soils. In particular, in soils heated at 300-500 degrees C there was momentary stimulating effect on substrate-induced respiration registered and on basal respiration values in soils burned at 150 degrees C and 300-400 degrees C. Four-month laboratory incubation of permafrost soils heated at different temperatures showed stimulation of microbial activity in first several days after inoculation due to high substrate availability after heating. Then soon after that soil microbial community started to be depleted on substrate because of decreasing water-soluble organic carbon, C and N content and it continued to the end of incubation. (C) 2016 Elsevier B.V. All rights reserved.

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VN Sukachev Inst Forest SB RAS, 50-28 Akad Gorodok, Krasnoyarsk 660036, Russia.

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Masyagina, O. V.; Tokareva, I. V.; Prokushkin, A. S.; Russian Science Foundation [14-24-00113]

/ N. Bischoff [et al.] // Biogeosciences. - 2017. - Vol. 14, Is. 10. - P2627-2640, DOI 10.5194/bg-14-2627-2017. - Cited References:58. - Financial support was provided by the German Federal Ministry of Education and Research (BMBF) in the framework of the KULUNDA project (01 LL 0905). Olga Shibistova and Georg Guggenberger appreciate funding from the Russian Ministry of Education and Science (No. 14.B25.31.0031). We thank the entire KULUNDA team for great collaboration and good team spirit. We are thankful to all farmers of the Kulunda steppe for collaboration during sampling and Lukas Gerhard for indispensable assistance in the field. Thanks for laboratory assistance to Silke Bokeloh, Elke Eichmann-Prusch, Roger-Michael Klatt, Pieter Wiese and Fabian Kalks, while Leopold Sauheitl is appreciated for guidance in the laboratory. Andrea Hartmann is acknowledged for helpful support on the scanning electron microscope. Thanks to Norman Gentsch for valuable scientific discussions on the manuscript, while we acknowledge Frank Schaarschmidt for statistical support. We thank two anonymous reviewers for valuable suggestions on the manuscript. The publication of this article was funded by the Open Access Fund of Leibniz Universitat Hannover. . - ISSN 1726-4170. - ISSN 1726-4189РУБ Ecology + Geosciences, Multidisciplinary

Аннотация: Macro-aggregates especially in agricultural steppe soils are supposed to play a vital role for soil organic carbon (OC) stabilization at a decadal timescale. While most research on soil OC stabilization in steppes focused on North American prairie soils of the Great Plains with information mainly provided by short-term incubation experiments, little is known about the agricultural steppes in southwestern Siberia, though they belong to the greatest conversion areas in the world and occupy an area larger than that in the Great Plains. To quantify the proportion of macro-aggregate-protected OC under different land use as function of land use intensity and time since land use change (LUC) from pasture to arable land in Siberian steppe soils, we determined OC mineralization rates of intact (250-2000 mu m) and crushed (250 mu m) macro-aggregates in long-term incubations over 401 days (20 degrees C; 60% water holding capacity) along two agricultural chronosequences in the Siberian Kulunda steppe. Additionally, we incubated bulk soil (2000 mu m) to determine the effect of LUC and subsequent agricultural use on a fast and a slow soil OC pool (labile vs. more stable OC), as derived from fitting exponential-decay models to incubation data. We hypothesized that (i) macro-aggregate crushing leads to increased OC mineralization due to an increasing microbial accessibility of a previously occluded labile macroaggregate OC fraction, and (ii) bulk soil OC mineralization rates and the size of the fast OC pool are higher in pasture than in arable soils with decreasing bulk soil OC mineralization rates and size of the fast OC pool as land use intensity and time since LUC increase. Against our hypothesis, OC mineralization rates of crushed macro-aggregates were similar to those of intact macro-aggregates under all land use regimes. Macro-aggregate-protected OC was almost absent and accounted for 1% of the total macro-aggregate OC content and to a maximum of 8 +/- 4% of mineralized OC. In accordance to our second hypothesis, highest bulk soil OC mineralization rates and sizes of the fast OC pool were determined under pasture, but mineralization rates and pool sizes were unaffected by land use intensity and time since LUC. However, at one chronosequence mean residence times of the fast and slow OC pool tended to decrease with increasing time since establishment of arable use. We conclude that the tillage-induced breakdown of macro-aggregates has not reduced the OC contents in the soils under study. The decline of OC after LUC is probably attributed to the faster soil OC turnover under arable land as compared to pasture at a reduced plant residue input.

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Leibniz Univ Hannover, Inst Soil Sci, D-30419 Hannover, Germany.
Martin Luther Univ Halle Wittenberg, Soil Sci & Soil Protect, D-06120 Halle, Germany.
Russian Acad Sci, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Inst Water & Environm Problems, Siberian Branch, Barnaul 656038, Russia.
Altai State Univ, Fac Biol, Barnaul 656049, Russia.
Johann Heinrich von Thunen Inst, Inst Climate Smart Agr, D-38116 Braunschweig, Germany.

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Bischoff, Norbert; Mikutta, Robert; Shibistova, Olga; Puzanov, Alexander; Silanteva, Marina; Grebennikova, Anna; Fuss, Roland; Guggenberger, Georg; German Federal Ministry of Education and Research (BMBF) of the KULUNDA project [01 LL 0905]; Russian Ministry of Education and Science [14.B25.31.0031]; Open Access Fund of Leibniz Universitat Hannover

/ M. I. Makarov [et al.] // Eurasian Soil Sci. - 2017. - Vol. 50, Is. 5. - P549-558, DOI 10.1134/S1064229317030085 . - ISSN 1064-2293
Аннотация: Concentrations of carbon and nitrogen extractable by 0.05 M K2SO4 (Cext and Next, respectively) in 14 soils of different ecosystems vary from 16 to 205 and from 4 to 53 mg/kg, respectively. The portion of Cext in soil organic matter is 0.06 to 0.38% of total carbon, and the portion of Next is 0.12–1.05% of total nitrogen. The storage of samples and their preparation to analysis differently affect the extractability of elements. The concentration of Cext is less variable than the concentration of Next. An increase in C extractability (by 1.4–6.7 times) is a common feature of all soils under drying; at the following incubation of dried soils, the extractability of C decreases by 28–56%. The extractability of N increases not only under drying (by 1.5–7.1 times) and the following incubation of samples (by 25–60% to 2–3 times), but also under freezing of most soils and at the incubation of fresh and defrozen samples. A close direct correlation is observed between the initial water content of soil and the relative increase in C extractability under drying and N extractability under freezing and drying. © 2017, Pleiades Publishing, Ltd.

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Lomonosov Moscow State University, Moscow, Russian Federation
Sukachev Institute of Forest Research, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

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Makarov, M. I.; Kuznetsova, E. Y.; Malysheva, T. I.; Maslov, M. N.; Menyailo, O. V.

/ Z. K. Zhu [et al.] // Plant Soil. - 2017. - Vol. 416, Is. 1-2. - P243-257, DOI 10.1007/s11104-017-3210-4. - Cited References:62. - The present study was supported by the National Natural Science Foundation of China (41522107; 41501321), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB15020401), and the Recruitment Program of High-End Foreign Experts of the State Administration of Foreign Experts Affairs, awarded to Prof. Georg Guggenberger (GDT20164300013). We thank the Public Service Technology Center, Institute of Subtropical Agriculture, Chinese Academy of Sciences for technical assistance. . - ISSN 0032-079X. - ISSN 1573-5036РУБ Agronomy + Plant Sciences + Soil Science

Аннотация: The turnover of plant- and microbial- derived carbon (C) plays a significant role in the soil organic C (SOC) cycle. However, there is limited information about the turnover of the recently photosynthesized plant- and soil microbe-derived C in paddy soil. We conducted an incubation study with four different C-13-labeled substrates: rice shoots (Shoot-C), rice roots (Root-C), rice rhizodeposits (Rhizo-C), and microbe-assimilated C (Micro-C). Shoot- and Root-C were initially rapidly transformed into the dissolved organic C (DOC) pool, while their recovery in microbial biomass C (MBC) and SOC increased with incubation time. There were 0.05%, 9.8% and 10.0% of shoot-C, and 0.06%, 15.9% and 16.5% of root-C recovered in DOC, MBC and SOC pools, respectively at the end of incubation. The percentages of Rhizo- and Micro-C recovered in DOC, MBC, and SOC pools slowly decreased over time. Less than 0.1% of the Rhizo- and Micro-C recovered in DOC pools at the end of experiment; while 45.2% and 33.8% of Rhizo- and Micro-C recovered in SOC pools. Shoot- and Root-C greatly increased the amount of C-13-PLFA in the initial 50 d incubation, which concerned PLFA being indicative for fungi and actinomycetes while those assigning gram-positive bacteria decreased. The dynamic of soil microbes utilizing Rhizo- and Micro-C showed an inverse pattern than those using Shoot- and Root-C. Principal component analysis of C-13-PLFA showed that microbial community composition shifted obviously in the Shoot-C and Root-C treatments over time, but that composition changed little in the Rhizo-C and Micro-C treatments. The input C substrates drive soil microbial community structure and function with respect to carbon stabilization. Rhizodeposited and microbial assimilated C have lower input rates, however, they are better stabilized than shoot- and root-derived C, and thus are preferentially involved in the formation of stable SOC in paddy soils.

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Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.
Chinese Acad Sci, Inst Subtrop Agr, Changsha Res Stn Agr & Environm Monitoring, Changsha 410125, Hunan, Peoples R China.
Leibniz Univ Hannover, Inst Soil Sci, D-30419 Hannover, Germany.
SB RAS, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zhu, Zhenke; Ge, Tida; Hu, Yajun; Zhou, Ping; Wang, Tingting; Shibistova, Olga; Guggenberger, Georg; Su, Yirong; Wu, Jinshui; National Natural Science Foundation of China [41522107, 41501321]; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB15020401]; Recruitment Program of High-End Foreign Experts of the State Administration of Foreign Experts Affairs [GDT20164300013]

/ L. Mukhortova [et al.] // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM : International Multidisciplinary Scientific Geoconference, 2017. - Vol. 17: 17th International Multidisciplinary Scientific Geoconference, SGEM 2017 (29 June 2017 through 5 July 2017, ) Conference code: 130796, Is. 32. - P829-836, DOI 10.5593/sgem2017/32/S13.107 . -
Аннотация: The aim of this study was experimental assessing the emission of greenhouse gasses (CO2, CH4) from decomposed coarse woody debris in northern boreal forests of Siberia, where the main forest-forming species is larch (Larix gmelinii (Rupr.) Rupr.). Logs samples were collected in northern boreal larch forests of Central Evenkia (64°N, 100°E) at different stages of decomposition and placed in gas-tight boxes. Gas samples were measured in dynamics: at the beginning of the experiment and after 3 hour, 24 hours, after 3 and 6 days of incubation using Picarro G2201-i analyzer. Totally 12 samples were measured. Samples were divided into the three decay classes, based on visual and physical properties. The main basis for division is wood density and presence of bark and branches: decomposition class I - wood has not lost its solidity, stems have bark and branches; decomposition class II - wood has lost some of its solidity, bark easily flakes from wood, but bark and branches are presented on the stems; decomposition class III - wood has almost fully lost its initial solidity, some bark and large branches remain on the stems. It was found that carbon dioxide concentration increased gradually during incubation for logs at all decomposition stages. Coarse woody debris at early stages of decomposition produced 3.3-11.4 µg CO2 cm-3 h-1 for the decomposition class I and 1.9-6.2 µg CO2 cm-3 h-1 for the decomposition class II. Flux of carbon dioxide from coarse woody debris at the advanced stage of decomposition (decomposition class III) was significantly higher and comprised 0.9-12.4 µg CO2 cm-3 h-1. Carbon dioxide emission showed close correlation with temperature, class of decomposition and on type of rot (white or brown rot fungi consortia decomposed logs). Concentration of methane showed gradual increase of its concentration during 6 days incubation (from 1.84 to 2.87 and 3.57 ppm for I and II decomposition classes).Rate of methane increasing was dependent on temperature. If at the temperature +5°C increasing of methane concentration was slow and was observed only for decomposition class I and II, at the temperature +25°C logs of all decomposition classes increased concentration of methane from 1.82-1.84 ppm of the initial measurement to 2.06-2.87 ppm after the 6 days of incubation. © SGEM2017. All Rights Reserved.

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Держатели документа:
V.N. Sukachev Institute of Forest FIC SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Mukhortova, L.; Evgrafova, S.; Meteleva, M.; Krivobokov, L.

/ S. Evgrafova [et al.] // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM : International Multidisciplinary Scientific Geoconference, 2017. - Vol. 17: 17th International Multidisciplinary Scientific Geoconference, SGEM 2017 (29 June 2017 through 5 July 2017, ) Conference code: 130796, Is. 32. - P257-264, DOI 10.5593/sgem2017/32/S13.034 . -
Аннотация: We aimed at identifying the microbial response and associated release of CO2 and CH4 in/from thawing soil that has been permanently frozen. For that we performed an in situ field-based incubation experiment in a rim of ice-wedge polygon on Samoylov island, Lena Delta, Russia (72°22’N, 126°28’E). Frozen "buried' organic matter were taken from eroded Lena river bank and transferred to the soil surface in a rim of ice-wedge polygon. The principle includes that formerly frozen soil is moved to the active layer, but still residing in the subsoil in order to mimic cryoturbation processes. The mean seasonal methane efflux from soil surface with the transplaced permafrost soil, as measured in the vegetation period after experiment set up, was 0.55±0.07 mg CH4 m-2 h-1; whereas the mean seasonal methane efflux from plots without buried organic material (i.e., disturbance control) was 0.50±0.02 mg CH4 m-2 h-1. Hence, differences were minor. CO2 emission measured by dark chambers did not differ in magnitude during 4 weeks from the beginning of the vegetation period, and then was approximately 1.5 times larger in plots containing organic material. The release of CO2 from soil was mainly responding to soil temperature, as the Pearson's coefficient for correlation between heterotrophic respiration rate and soil and air temperature was r=0.63, r=0.38, respectively. We conclude that the heterotrophic part of microbial community needs some period for adaptation to the chemical properties of the introduced organic matter (approximately 3-4 weeks). Consequently, due to the short vegetation period in this ecosystem we expect that the acceleration of carbon release is possibly not pronounced. © SGEM2017. All Rights Reserved.

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Держатели документа:
V.N. Sukachev Institute of Forest FIC SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Soil Science, Leibniz University of Hannover, Germany

Доп.точки доступа:
Evgrafova, S.; Novikov, O.; Meteleva, M.; Guggenberger, G.

/ Z. Zhu [et al.] // Soil Biol. Biochem. - 2018. - Vol. 121. - P67-76, DOI 10.1016/j.soilbio.2018.03.003 . - ISSN 0038-0717
Аннотация: Nitrogen (N) and phosphorus (P) availability plays a crucial role in carbon (C) cycling in terrestrial ecosystems. However, the C:N:P stoichiometric regulation of microbial mineralization of plant residues and its impact on the soil priming effect (PE), measured as CO2 and CH4 emission, in paddy soils remain unclear. In this study, the effect of soil C:N:P stoichiometry (regulated by the application of N and P fertilizers) on the mineralization of 13C-labelled rice straw and the subsequent PE was investigated in a 100-day incubation experiment in flooded paddy soil. N and P additions increased straw mineralization by approximately 25% and 10%, respectively. Additions of both N and P led to higher CO2 efflux, but lower CH4 emission. With an increase in the ratios of DOC:NH4 +-N, DOC:Olsen P, and microbial biomass C:N, 13CO2 efflux increased exponentially to a maximum. Compared with sole straw addition, exclusive N addition led to a weaker PE for CO2 emission, whereas exclusive P addition induced a stronger PE for CO2 emission. In contrast, CH4 emitted from native soil organic matter (SOM) was reduced by 7.4% and 46.1% following P and NP application, respectively. Structural equation models suggest that available N had dominant and direct positive effects, whereas microbial biomass stoichiometry mainly exerted negative indirect effects on PE. The stoichiometry of soil enzyme activity directly down-regulated CH4 emission from SOM. Microbes obviously regulate soil C turnover via stoichiometric flexibility to maintain an elemental stoichiometric balance between resources and microbial requirements. The addition of straw in combination with N and P fertilization in paddy soils could therefore meet microbial stoichiometric requirements and regulate microbial activity and extracellular enzyme production, resulting in co-metabolism of fresh C and native SOM. © 2018 Elsevier Ltd

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Держатели документа:
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, China
Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, China
Institute of Soil and Water Resources and Environmental Science, Zhejiang Provincial Key Laboratory of Agricultural Resources and Environment, Zhejiang University, Hangzhou, China
Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, China
Institute of Soil Science, Leibniz Universitat Hannover, Hannover, Germany
VN Sukachev Institute of Forest, SB-RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Zhu, Z.; Ge, T.; Luo, Y.; Liu, S.; Xu, X.; Tong, C.; Shibistova, O.; Guggenberger, G.; Wu, J.

/ Y. H. Liu [et al.] // Appl. Soil Ecol. - 2018. - Vol. 127. - P51-57, DOI 10.1016/j.apsoil.2018.02.012. - Cited References:53. - This study was financially supported by the National Key Research and Development program (2016YFD0300902), the National Natural Science Foundation of China (41522107; 41671253; 31470629), Chinese Academy of Sciences President's International Fellowship Initiative to Georg Guggenberger (2018VCA0031) and Youth Innovation Team Project of ISA, CAS (2017QNCXTD_GTD). We also thank to Public Service Technology Center, Institute of Subtropical Agriculture, Chinese Academy of Sciences for the technique support and Jiangxi Normal University domestic (abroad) visit programs funded support. . - ISSN 0929-1393. - ISSN 1873-0272РУБ Soil Science

Аннотация: Paddy soils have experienced intensive fertilization in recent decades. However, our understanding of the effects of fertilization on the carbon (C) cycle remains incomplete. In the present study, we investigated soil organic matter (SOM) decomposition in a 60-day incubation in response to N, P, K, Ca, and S addition to nutrient-limited paddy soil at three low and three high concentrations. High levels of nutrient addition decreased CO2 emission, qCO(2), and microbial biomass. CO2 emissions increased (12-17%) owing to low levels of nutrient addition, whereas it decreased (3-21%) in response to high levels of nutrient addition. Microbial biomass and nutrient turnover rates increased after low levels of nutrient addition. Positive priming effect occurs under nutrient-limited conditions owing to the stimulation of microbial biomass production after low amount of exogenous nutrient input. In contrast, high levels of nutrient addition decreased microbial biomass and net N mineralization. This high N, P, K, Ca, and S addition could satisfy the needs of microbial growth, thereby decreasing the dependency of the organisms on the original nutrients from SOM decomposition. Therefore, negative priming was observed after high-level nutrient addition. In conclusion, intensive fertilization (with N, P, K, Ca, and S) reduces SOM decomposition through increased microbial turnover in paddy soils, which might positively affect C sequestration.

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Jiangxi Normal Univ, Coll Life Sci, Nanchang 330022, Jiangxi, Peoples R China.
Chinese Acad Sci, Key Lab Agroecol Proc Subtrop Reg, Inst Subtrop Agr, Beijing 410125, Hunan, Peoples R China.
Chinese Acad Sci, Changsha Res Stn Agr & Environm Monitoring, Inst Subtrop Agr, Beijing 410125, Hunan, Peoples R China.
Univ Goettingen, Dept Agr Soil Sci, Dept Soil Sci Temperate Ecosyst, D-37077 Gottingen, Germany.
Cent South Univ Forestry & Technol, Coll Environm Sci & Engn, Changsha 410004, Hunan, Peoples R China.
Leibniz Univ Hannover, Inst Soil Sci, D-30419 Hannover, Germany.
SB RAS, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Liu, Yuhuai; Zang, Huadong; Ge, Tida; Bai, Jing; Lu, Shunbao; Zhou, Ping; Peng, Peiqing; Shibistova, Olga; Zhu, Zhenke; Wu, Jinshui; Guggenberger, Georg; National Key Research and Development program [2016YFD0300902]; National Natural Science Foundation of China [41522107, 41671253, 31470629]; Chinese Academy of Sciences President's International Fellowship Initiative [2018VCA0031]; Youth Innovation Team Project of ISA, CAS [2017QNCXTD_GTD]; Jiangxi Normal University domestic (abroad) visit programs

/ N. Gentsch [et al.] // Glob. Change Biol. - 2018. - Vol. 24, Is. 8. - P3401-3415, DOI 10.1111/gcb.14316. - Cited References:91. - German Federal Ministry of Education and Research, Grant/Award Number: 03F0616A; Russian Ministry of Education and Science, Grant/Award Number: 14.B25.31.0031; Austrian Science Fund, Grant/Award Number: FWF - I370-B17; Czech Science Foundation, Grant/Award Number: n.16-18453S . - ISSN 1354-1013. - ISSN 1365-2486РУБ Biodiversity Conservation + Ecology + Environmental Sciences

Аннотация: Climate change in Arctic ecosystems fosters permafrost thaw and makes massive amounts of ancient soil organic carbon (OC) available to microbial breakdown. However, fractions of the organic matter (OM) may be protected from rapid decomposition by their association with minerals. Little is known about the effects of mineral-organic associations (MOA) on the microbial accessibility of OM in permafrost soils and it is not clear which factors control its temperature sensitivity. In order to investigate if and how permafrost soil OC turnover is affected by mineral controls, the heavy fraction (HF) representing mostly MOA was obtained by density fractionation from 27 permafrost soil profiles of the Siberian Arctic. In parallel laboratory incubations, the unfractionated soils (bulk) and their HF were comparatively incubated for 175 days at 5 and 15 degrees C. The HF was equivalent to 70 +/- 9% of the bulk CO2 respiration as compared to a share of 63 +/- 1% of bulk OC that was stored in the HF. Significant reduction of OC mineralization was found in all treatments with increasing OC content of the HF (HF-OC), clay-size minerals and Fe or Al oxyhydroxides. Temperature sensitivity (Q10) decreased with increasing soil depth from 2.4 to 1.4 in the bulk soil and from 2.9 to 1.5 in the HF. A concurrent increase in the metal-to-HF-OC ratios with soil depth suggests a stronger bonding of OM to minerals in the subsoil. There, the younger C-14 signature in CO2 than that of the OC indicates a preferential decomposition of the more recent OM and the existence of a MOA fraction with limited access of OM to decomposers. These results indicate strong mineral controls on the decomposability of OM after permafrost thaw and on its temperature sensitivity. Thus, we here provide evidence that OM temperature sensitivity can be attenuated by MOA in permafrost soils.

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Leibniz Univ Hannover, Inst Soil Sci, Hannover, Germany.
Univ Vienna, Dept Microbiol & EcoSyst Sci, Vienna, Austria.
Austrian Polar Res Inst, Vienna, Austria.
Stockholm Univ, Dept Environm Sci & Analyt Chem, S-10691 Stockholm, Sweden.
Stockholm Univ, Bolin Ctr Climate Res, S-10691 Stockholm, Sweden.
Martin Luther Univ Halle Wittenberg, Soil Sci & Soil Protect, Halle, Germany.
Univ South Bohemia, Dept Ecosyst Biol, Ceske Budejovice, Czech Republic.
Max Planck Inst Biogeochem, Jena, Germany.
Fed Inst Geosci & Nat Resources BGR, Hannover, Germany.
Univ Bayreuth, Soil Ecol, Bayreuth, Germany.
Leibniz Univ Hannover, Inst Biostat, Hannover, Germany.
Siberian Branch Russian Acad Sci, VN Sukachev Inst Forest, Krasnoyarsk, Russia.
Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA.
Univ Vienna, Dept Ecogen & Syst Biol, Vienna, Austria.
Ernst Moritz Arndt Univ Greifswald, Inst Microbiol, Greifswald, Germany.
Univ Bergen, Ctr Geobiol, Dept Biol, Bergen, Norway.
Ctr Geomicrobiol, Dept Biosci, Aarhus, Denmark.
Siberian Branch Russian Acad Sci, Ctr Siberian Bot Garden, Novosibirsk, Russia.
Thunen Inst Climate Smart Agr, Braunschweig, Germany.

Доп.точки доступа:
Gentsch, Norman; Wild, Birgit; Mikutta, Robert; Capek, Petr; Diakova, Katka; Schrumpf, Marion; Turner, Stephanie; Minnich, Cynthia; Schaarschmidt, Frank; Shibistova, Olga; Schnecker, Joerg; Urich, Tim; Gittel, Antje; Santruckova, Hana; Barta, Jiri; Lashchinskiy, Nikolay; Fuss, Roland; Richter, Andreas; Guggenberger, Georg; Schnecker, Jorg; German Federal Ministry of Education and Research [03F0616A]; Russian Ministry of Education and Science [14.B25.31.0031]; Austrian Science Fund [FWF - I370-B17]; Czech Science Foundation [n.16-18453S]

/ S. Evgrafova [et al.] // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM : International Multidisciplinary Scientific Geoconference, 2018. - Vol. 18: 18th International Multidisciplinary Scientific Geoconference, SGEM 2018 (2 July 2018 through 8 July 2018, ) Conference code: 142896, Is. 3.2. - P213-218, DOI 10.5593/sgem2018/3.2/S13.028 . -
Аннотация: A fundamental research question related to the impact of thawing permafrost on global change is, how fast organic matter in the thawing permafrost can be converted to CO2 and CH4 and released into the atmosphere. Current estimates on the degradability of thawing organic matter in permafrost are based on incubation studies which are highly artificial and probably overestimate the greenhouse gas production under in situ conditions. We aimed at identifying the microbial response and associated release of CO2 and CH4 from thawing soil that has previously been permanently frozen. For this, we performed an in situ field-based incubation experiment in a rim of an ice-wedge polygon on Samoylov island in the Lena River Delta, Russia, at 72°22’N, 126°28’E. We moved formerly frozen soil to the active layer. This material was either placed partly in the subsoil, to mimic the cryoturbation processes, or was exposed to the soil surface to simulate an eroded river bank. Data from the incubation experiment showed low intensity of gas emission which indicates a weak involvement of the buried soil in the present-day processes of microbial decomposition. © SGEM 2018.

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Держатели документа:
V.N. Sukachev Institute, of Forest FIC SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Soil Science, Leibniz University of Hannover, Germany

Доп.точки доступа:
Evgrafova, S.; Novikov, O.; Meteleva, M.; Guggenberger, G.

/ S. Evgrafova [et al.] // International Multidisciplinary Scientific GeoConference Surveying Geology and Mining Ecology Management, SGEM : International Multidisciplinary Scientific Geoconference, 2019. - Vol. 19: 19th International Multidisciplinary Scientific Geoconference, SGEM 2019 (30 June 2019 through 6 July 2019, ) Conference code: 150487, Is. 3.2. - P293-300, DOI 10.5593/sgem2019/3.2/S13.039 . -
Аннотация: Methanotrophic bacteria are unique group of microorganisms structurally and functionally adapted to use methane as a source of carbon, which is of great interest due to their ability to oxidize atmospheric methane. Methanotrophs are known to associate with mosses, which provide bacteria by habitat and protection. Methanotrophic bacteria provide mosses with carbon dioxide resulting of methane oxidation, whose content in moss tissues can reach 32%. We studied mosses and lichens sampled in Eastern Siberia permafrost ecosystems for methane oxidizing ability of associated bacteria, at concentrations of methane close to atmospheric. The consumption of methane in consortia of mosses and lichens and associated microorganisms was measured in laboratory incubation experiments. The methanotrophic activity registrated using gas analyzer Picarro 2201-i (Picarro Inc., USA) as a shift in the isotopic composition ?13C in methane. It was shown that samples collected in permafrost soils have a larger ability to methanotrophy than sample collected in non-permafrost soils. In addition, we measured methanotrophic ability of the individual species of mosses and lichens. It has been shown that methanotrophs associated with lichens Cladonia stelaris and Cetraria laevigata have great methanotrophic ability on a level of methanotrophs associated with mosses Rhytidium rugosum and Dicranum polysetum. © SGEM2019 All Rights Reserved.

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Держатели документа:
V.N. Sukachev Institute of Forest, FRC KSC SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Evgrafova, S.; Kadutskiy, V.; Mukhortova, L.; Prudnikova, S.

/ J. Cui, Z. Zhu, X. Xu [et al.] // Soil Biol. Biochem. - 2020. - Vol. 142. - Ст. 107720, DOI 10.1016/j.soilbio.2020.107720 . - ISSN 0038-0717
Аннотация: The impact of increasing amounts of labile C input on priming effects (PE) on soil organic matter (SOM) mineralization remains unclear, particularly under anoxic conditions and under high C input common in microbial hotspots. PE and their mechanisms were investigated by a 60-day incubation of three flooded paddy soils amended with13C-labeled glucose equivalent to 50–500% of microbial biomass C (MBC). PE (14–55% of unamended soil) peaked at moderate glucose addition rates (i.e., 50–300% of MBC). Glucose addition above 300% of MBC suppressed SOM mineralization but intensified microbial N acquisition, which contradicted the common PE mechanism of accelerating SOM decomposition for N-supply (frequently termed as “N mining”). Particularly at glucose input rate higher than 3 g kg?1 (i.e., 300–500% of MBC), mineral N content dropped on day 2 close to zero (1.1–2.5 mg N kg?1) because of microbial N immobilization. To cope with the N limitation, microorganisms greatly increased N-acetyl glucosaminidase and leucine aminopeptidase activities, while SOM decomposition decreased. Several discrete peaks of glucose-derived CO2 (contributing >80% to total CO2) were observed between days 13–30 under high glucose input (300–500% of MBC), concurrently with CH4 peaks. Such CO2 dynamics was distinct from the common exponential decay pattern, implicating the recycling and mineralization of 13C-enriched microbial necromass driven by glucose addition. Therefore, N recycling from necromass was hypothesized as a major mechanism to alleviate microbial N deficiency without SOM priming under excess labile C input. Compound-specific 13C-PLFA confirmed the redistribution of glucose-derived C among microbial groups, i.e., necromass recycling. Following glucose input, more than 4/5 of total 13C-PLFA was in the gram-negative and some non-specific bacteria, suggesting these microorganisms as r-strategists capable of rapidly utilizing the most labile C. However, their 13C-PLFA content decreased by 70% after 60 days, probably as a result of death of these r-strategists. On the contrary, the 13C-PLFA in gram-positive bacteria, actinomycetes and fungi (K-strategists) was initially minimal but increased by 0.5–5 folds between days 2 and 60. Consequently, the necromass of dead r-strategists provided a high-quality C–N source to the K-strategists. We conclude that under severe C excess, N recycling from necromass is a much more efficient microbial strategy to cover the acute N demand than N acquisition from the recalcitrant SOM. © 2020 Elsevier Ltd

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Держатели документа:
Key Laboratory of Agro-ecological Processes in Subtropical Region & Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China
State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Gongdong, 510640, China
Jiangsu Provincial Key Laboratory for Bioresources of Coastal Saline Soils, Jiangsu Coastal Biological Agriculture Synthetic Innovation Center, Yancheng Teachers' University, Yancheng, 224002, China
Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing, 100101, China
School of Environment, Natural Resources & Geography, Bangor University, Gwynedd, LL57 2UW, United Kingdom
Department of Agricultural Soil Science, Department of Soil Science of Temperate Ecosystems, University of G?ttingen, G?ttingen, Germany
Institute of Environmental Sciences, Kazan Federal University, Kazan, 420049, Russian Federation
Agro-Technological Institute, RUDN University, Moscow, 117198, Russian Federation
Departamento de Ciencias Quimicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile
VN Sukachev Institute of Forest, SB-RAS, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Cui, J.; Zhu, Z.; Xu, X.; Liu, S.; Jones, D. L.; Kuzyakov, Y.; Shibistova, O.; Wu, J.; Ge, T.

/ J. Cui, Z. K. Zhu, X. L. Xu [et al.] // Soil Biol. Biochem. - 2020. - Vol. 142. - Ст. 107720, DOI 10.1016/j.soilbio.2020.107720. - Cited References:80. - The study was supported by the National Key Research and Development Program of China (2017YFD0800104), the National Natural Science Foundation of China (41430860, 41771337, 41977093 and 31872695), State Key Laboratory of Organic Geochemistry, GIGCAS (SKLOG-201728), Hunan Province Base for Scientific and Technological Innovation Cooperation (2018WK4012), the Youth Innovation Team Project of Institute of Subtropical Agriculture, Chinese Academy of Sciences (2017QNCXTD_GTD), NSFC-RFBR joint project (N 19-54-53026) and Innovation Groups of National Natural Science Foundation of Hunan Province (2019JJ10003). We thank the Public Service Technology Center, Institute of Subtropical Agriculture, Chinese Academy of Sciences for technical assistance. The publication was supported by the Government Program of Competitive Growth of Kazan Federal University and with the support of the "RUDN University program 5-100." . - ISSN 0038-0717РУБ Soil Science

Аннотация: The impact of increasing amounts of labile C input on priming effects (PE) on soil organic matter (SOM) mineralization remains unclear, particularly under anoxic conditions and under high C input common in microbial hotspots. PE and their mechanisms were investigated by a 60-day incubation of three flooded paddy soils amended with(13)C-labeled glucose equivalent to 50-500% of microbial biomass C (MBC). PE (14-55% of unamended soil) peaked at moderate glucose addition rates (i.e., 50-300% of MBC). Glucose addition above 300% of MBC suppressed SOM mineralization but intensified microbial N acquisition, which contradicted the common PE mechanism of accelerating SOM decomposition for N-supply (frequently termed as "N mining"). Particularly at glucose input rate higher than 3 g kg(-1) (i.e., 300-500% of MBC), mineral N content dropped on day 2 close to zero (1.1-2.5 mg N kg(-1)) because of microbial N immobilization. To cope with the N limitation, microorganisms greatly increased N-acetyl glucosaminidase and leucine aminopeptidase activities, while SOM decomposition decreased. Several discrete peaks of glucose-derived CO2 (contributing >80% to total CO2) were observed between days 13-30 under high glucose input (300-500% of MBC), concurrently with CH4 peaks. Such CO2 dynamics was distinct from the common exponential decay pattern, implicating the recycling and mineralization of C-13-enriched microbial necromass driven by glucose addition. Therefore, N recycling from necromass was hypothesized as a major mechanism to alleviate microbial N deficiency without SOM priming under excess labile C input. Compound-specific C-13-PLFA confirmed the redistribution of glucose-derived C among microbial groups, i.e., necromass recycling. Following glucose input, more than 4/5 of total C-13-PLFA was in the gram-negative and some non-specific bacteria, suggesting these microorganisms as r-strategists capable of rapidly utilizing the most labile C. However, their C-13-PLFA content decreased by 70% after 60 days, probably as a result of death of these r-strategists. On the contrary, the C-13-PLFA in gram-positive bacteria, actinomycetes and fungi (K-strategists) was initially minimal but increased by 0.5-5 folds between days 2 and 60. Consequently, the necromass of dead r-strategists provided a high-quality C-N source to the K-strategists. We conclude that under severe C excess, N recycling from necromass is a much more efficient microbial strategy to cover the acute N demand than N acquisition from the recalcitrant SOM.

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Держатели документа:
Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.
Chinese Acad Sci, Inst Subtrop Agr, Changsha Res Stn Agr & Environm Monitoring, Changsha 410125, Hunan, Peoples R China.
Chinese Acad Sci, State Key Lab Organ Geochem, Guangzhou Inst Geochem, Gongdong 510640, Peoples R China.
Yancheng Teachers Univ, Jiangsu Prov Key Lab Bioresources Coastal Saline, Jiangsu Coastal Biol Agr Synthet Innovat Ctr, Yancheng 224002, Peoples R China.
Chinese Acad Sci, Key Lab Ecosyst Network Observat & Modeling, Inst Geog Sci & Nat Resources Res, Beijing, Peoples R China.
Bangor Univ, Sch Environm Nat Resources & Geog, Bangor LL57 2UW, Gwynedd, Wales.
Univ Gottingen, Dept Agr Soil Sci, Dept Soil Sci Temperate Ecosyst, Gottingen, Germany.
Kazan Fed Univ, Inst Environm Sci, Kazan 420049, Russia.
RUDN Univ, Agrotechnol Inst, Moscow 117198, Russia.
Univ La Frontera, Dept Ciencias Quim & Recursos Nat, Temuco, Chile.
RAS, SB, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Cui, Jun; Zhu, Zhenke; Xu, Xingliang; Liu, Shoulong; Jones, Davey L.; Kuzyakov, Yakov; Shibistova, Olga; Wu, Jinshui; Ge, Tida; National Key Research and Development Program of China [2017YFD0800104]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41430860, 41771337, 41977093, 31872695]; State Key Laboratory of Organic Geochemistry, GIGCAS [SKLOG-201728]; Hunan Province Base for Scientific and Technological Innovation Cooperation [2018WK4012]; Youth Innovation Team Project of Institute of Subtropical Agriculture, Chinese Academy of Sciences [2017QNCXTD_GTD]; NSFC-RFBR joint project [N 19-54-53026]; Innovation Groups of National Natural Science Foundation of Hunan Province [2019JJ10003]; Government Program of Competitive Growth of Kazan Federal University; RUDN University program 5-100

/ X. M. Wei, Z. K. Zhu, Y. Liu [et al.] // Biol. Fertil. Soils. - 2020, DOI 10.1007/s00374-020-01468-7. - Cited References:78. - This study was financially supported by the National Natural Science Foundation of China (41430860, 41877104, and 41761134095); Innovative Research Groups of the Natural Science Foundation of Hunan Province (2019JJ10003); Natural Science Foundation of Hunan Province (2019JJ30028); the Youth Innovation Team Project of the Institute of Subtropical Agriculture, Chinese Academy of Sciences (2017QNCXTD_GTD); the Youth Innovation Promotion Association (2019357); the China Scholarship Council (201904910049); and the Chinese Academy of Sciences President's International Fellowship Initiative to Georg Guggenberger (2018VCA0031). . - Article in press. - ISSN 0178-2762. - ISSN 1432-0789РУБ Soil Science

Аннотация: Stoichiometric control of input substrate (glucose) and native soil organic C (SOC) mineralization was assessed by performing a manipulation experiment based on N or P fertilization in paddy soil. Glucose mineralization increased with nutrient addition up to 11.6% with combined N and P application compared with that without nutrient addition. During 100 days of incubation, approximately 4.5% of SOC was mineralized and was stimulated by glucose addition. Glucose and SOC mineralization increased exponentially with dissolved organic C (DOC):NH4+-N, DOC:Olsen P, and microbial biomass (MB)C:MBN ratios. The relative abundances of Clostridia and beta-Proteobacteria (r-strategists) were increased with combined C and NP application at the beginning of the experiment, while the relative abundances of Acidobacteria (K-strategists) were enhanced with the exhaustion of available resource at the end of incubation. The bacteria abundance and diversity were negatively related to the DOC:NH4+-N and DOC:Olsen P, which had direct positive effects (+ 0.63) on SOC mineralization. Combined glucose and NP application decreased the network density of the bacterial community. Moreover, P addition significantly decreased the negative associations among bacterial taxa, which suggested that microbial competition for nutrients was alleviated. The relative abundances of keystone species showed significant positive correlations with SOC mineralization in the soils without P application, revealing that microbes increased their activity for mining of limited nutrients from soil organic matter. Hence, bacteria shifted their community composition and their interactions to acquire necessary elements by increasing SOC mineralization to maintain the microbial biomass C:N:P stoichiometric balance in response to changes in resource stoichiometry.

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Держатели документа:
Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changde 410125, Hunan, Peoples R China.
Chinese Acad Sci, Inst Subtrop Agr, Changsha Res Stn Agr & Environm Monitoring, Changde 410125, Hunan, Peoples R China.
Univ Chinese Acad Sci, Beijing 100049, Peoples R China.
Zhejiang Univ, Inst Soil & Water Resources & Environm Sci, Zhejiang Prov Key Lab Agr Resources & Environm, Hangzhou 310058, Peoples R China.
Jiangxi Univ Sci & Technol, Sch Resources & Environm Engn, Ganzhou 341000, Peoples R China.
Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing 100101, Peoples R China.
Univ Vienna, Ctr Microbiol & Ecosyst Sci, A-1090 Vienna, Austria.
Leibniz Univ Hannover, Inst Soil Sci, D-30419 Hannover, Germany.
SB RAS, VN Sukachev Inst Forest, Krasnoyarsk, Russia.

Доп.точки доступа:
Wei, Xiaomeng; Zhu, Zhenke; Liu, Y.i.; Luo, Y.u.; Deng, Yangwu; Xu, Xingliang; Liu, Shoulong; Richter, Andreas; Shibistova, Olga; Guggenberger, Georg; Wu, Jinshui; Ge, Tida; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41430860, 41877104, 41761134095]; Innovative Research Groups of the Natural Science Foundation of Hunan Province [2019JJ10003]; Natural Science Foundation of Hunan ProvinceNatural Science Foundation of Hunan Province [2019JJ30028]; Youth Innovation Team Project of the Institute of Subtropical Agriculture, Chinese Academy of Sciences [2017QNCXTD_GTD]; Youth Innovation Promotion Association [2019357]; China Scholarship CouncilChina Scholarship Council [201904910049]; Chinese Academy of Sciences President's International Fellowship Initiative [2018VCA0031]