Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[] / I. N. Tret'yakova, E. V. Voroshilova // Russ. J. Dev. Biol. - 2014. - Vol. 45, Is. 2. - P93-100, DOI 10.1134/S1062360414020064 . - ISSN 1608-3326
Аннотация: Megagametophytes of Siberian pine were cultured on an in vitro culture medium 1/2 LV supplemented with growth regulators 2,4-dichlorophenoxyacetic acid (2,4-D) and benzylaminopurine (6-BAP) to form embryos. The competency of somatic cell of explants to embryogenesis manifested itself in an organized growth and polarity. A coenocyte consisting of long vacuolated cells was formed in the megagametophyte culture. Then, the migration of the nuclei to one of the poles of the cell, their division, and formation of embryoids was observed. The megagametophyte culture of the Siberian pine differed from the zygotic embryo culture by the absence of asymmetric division in the vacuolated cell. © 2014 Pleiades Publishing, Inc.

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Держатели документа:
Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, bld. 28, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Tret'yakova, I.N.; Voroshilova, E.V.

[Text] / K. V. Krutovsky [et al.] // In Vitro Cell. Dev. Biol.-Plant. - 2014. - Vol. 50, Is. 5. - P655-664, DOI 10.1007/s11627-014-9619-z. - Cited References: 77. - This study was supported by Research Grant No. 14.Y26.31.0004 from the Government of the Russian Federation. . - ISSN 1054-5476. - ISSN 1475-2689РУБ Plant Sciences + Cell Biology + Developmental Biology

Аннотация: The objective of this study was to obtain a genetically stable haploid in vitro-derived line from Siberian larch (Larix sibirica Ledeb.) using megagametophyte explants, which then could be used for different molecular genetic studies, including whole genome de novo sequencing. However, cytogenetic analysis and genotyping of 11 microsatellite loci showed high levels of genomic instability and a high frequency of mutation in the obtained megagametophyte-derived callus cultures. All cultures contained new mutations in one or more microsatellite loci.

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Держатели документа:
[Krutovsky, Konstantin V.] Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgen Inst, D-37077 Gottingen, Germany
[Krutovsky, Konstantin V.] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA
[Krutovsky, Konstantin V.] Russian Acad Sci, NI Vavilov Inst Gen Genet, Moscow 119333, Russia
[Krutovsky, Konstantin V.
Tretyakova, Iraida N.
Oreshkova, Nataliay V.
Vaganov, Eugene A.] Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia
[Tretyakova, Iraida N.
Oreshkova, Nataliay V.
Pak, Maria E.
Kvitko, Olga V.] Russian Acad Sci, Lab Forest Genet & Select, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia
[Vaganov, Eugene A.] Siberian Fed Univ, Dept Ecol & Environm Studies, Inst Econ Management & Environm Studies, Krasnoyarsk 660041, Russia
ИЛ СО РАН

Доп.точки доступа:
Krutovsky, K.V.; Tretyakova, I.N.; Oreshkova, N.V.; Pak, M.E.; Kvitko, O.V.; Vaganov, E.A.; Government of the Russian Federation [14.Y26.31.0004]

/ I. N. Tretyakova, A. S. Shuklina, M. E. Park [et al.] // Cytologia. - 2021. - Vol. 86, Is. 1. - P55-60, DOI 10.1508/CYTOLOGIA.86.55 . - ISSN 0011-4545
Аннотация: The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in the cells was studied with immunohistochemical analysis. It was shown that the formation of embryogenic cultures in both species is associated with the content of phytohormones and their localization in cells. In L. sibirica the content of IAA in ESM was about 100 times higher than in non-embryogenic calli. At the same time, a low ABA content was characteristic of embryogenic cultures. Non-embryogenic callus contains an increased content of cytokinins and ABA. The transition of somatic cells to the path of embryogenic development is characterized by elongation of cells, their asymmetric division, and localization of IAA at one of the ends of elongated cells. Nonembryogenic callus consisted of isodiametric, actively dividing cells. © 2021 The Japan Mendel Society.

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Держатели документа:
Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Federal Research Center Krasnoyarsk Science Center SB RAS, Akademgorodok 50/28, Krasnoyarsk, 660036, Russian Federation
State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China
Institute of Biology, Russian Academy of Sciences, Ufa Research Center RAS, pr-t Oktyabrya 69, Ufa, 450054, Russian Federation

Доп.точки доступа:
Tretyakova, I. N.; Shuklina, A. S.; Park, M. E.; Yang, L.; Akhiyarova, G. R.; Kudoyarova, G. R.

/ I. N. Tretyakova, A. S. Shuklina, M. E. Park [et al.] // Cytologia. - 2021. - Vol. 86, Is. 1. - P55-60, DOI 10.1508/cytologia.86.55. - Cited References:24. - The reported study was funded by RFBR, the Government of the Krasnoyarsk Territory and the Regional Science Foundation, project number 19-44-240009. . - ISSN 0011-4545РУБ Cell Biology + Genetics & Heredity

Аннотация: The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in the cells was studied with immunohistochemical analysis. It was shown that the formation of embryogenic cultures in both species is associated with the content of phytohormones and their localization in cells. In L. sibirica the content of IAA in ESM was about 100 times higher than in non-embryogenic calli. At the same time, a low ABA content was characteristic of embryogenic cultures. Non-embryogenic callus contains an increased content of cytokinins and ABA. The transition of somatic cells to the path of embryogenic development is characterized by elongation of cells, their asymmetric division, and localization of IAA at one of the ends of elongated cells. Non-embryogenic callus consisted of isodiametric, actively dividing cells.

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Держатели документа:
Russian Acad Sci, Fed Res Ctr, Sukachev Inst Forest, Siberian Branch,Krasnoyarsk Sci Ctr SB RAS, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.
Northeast Forestry Univ, Sch Forestry, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China.
Russian Acad Sci, Ufa Res Ctr RAS, Inst Biol, Pr T Oktyabrya 69, Ufa 450054, Russia.

Доп.точки доступа:
Tretyakova, Iraida N.; Shuklina, Alla S.; Park, Maria E.; Yang, Ling; Akhiyarova, Guzel R.; Kudoyarova, Guzel R.; RFBRRussian Foundation for Basic Research (RFBR); Government of the Krasnoyarsk Territory; Regional Science Foundation [19-44-240009]