Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ V. P. Zhelifonova [et al.] // Appl. Biochem. Microbiol. - 2019. - Vol. 55, Is. 3. - P277-283, DOI 10.1134/S0003683819030153. - Cited References:18 . - ISSN 0003-6838. - ISSN 1608-3024РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: Secondary metabolites of the basidiomycetes of Armillaria borealis Marxm. and Korhonen, A.cepistipes Velen., A. gallica Marxm., A. ostoyae (Romagn.) Herink, and A. sinapina Berube and Dessur isolated in Southern Siberia (Krasnoyarsk region and Tyva Republic) and in the Far East (Sikhote-Alin) were studied. Metabolites belonging to the class of protoilludene sesquiterpene aryl esters of the melleolides group have been identified in the species A. borealis, A. cepistipes, and A. sinapina. The strains differ in the spectrum of synthesized melleolides. A. borealis strain 74g synthesized a wider range of melleolides than other strains of the species: melleolides B, C, D and H, melledonals B and C, 5'-O-methylmelledonal, 13-hydroxy-5'-O-methylmelledonal, and armillarinin. It was shown that the composition of the medium influenced the amount of synthesized metabolites. All of the studied strains synthesizing melleolides exhibited a toxicogenic and phytopathogenic effect on seeds and seedlings of Picea abies (L.) H. Karst. and Abies sibirica Ledeb. A significant decrease in seed germination energy, laboratory and ground seed germination, the development of the root system of conifer seedlings was revealed. The maximum inhibitory effect was shown by the culture fluid of A. borealis 74g with the greatest amounts and variety of melleolides in the metabolome profile.

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Держатели документа:
Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Zhelifonova, V. P.; Antipova, T. V.; Litvinova, E. A.; Baskunov, B. P.; Litovka, Yu. A.; Pavlov, I. N.; Kozlovsky, A. G.

/ T. V. Antipova, V. P. Zhelifonova, Y. A. Litovka [et al.] // Appl. Biochem. Microbiol. - 2020. - Vol. 56, Is. 2. - P185-193, DOI 10.1134/S0003683820020039. - Cited References:30 . - ISSN 0003-6838. - ISSN 1608-3024РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: The composition of secondary metabolites from strains Heterobasidion genus isolated in central and western Siberia and in South Korea were studied. Morphological-cultural and molecular-genetic methods were used to assign the cultures to the species of H. annosum (Fr.) Bref. (five strains), H. abietinum Niemela & Korhonen (four strains), and H. ecrustosum Tokuda, T. Hatt. & Y.C. Give (one strain). Fomannoxin predominated in the metabolome profiles of three H. annosum strains and all H. abietinum strains. Two strains of H. annosum synthesized fomannoxin-related compounds: 2-(2-hydroxypropan-2-yl)-2,3-dihydrobenzofuran-5-carbaldehyde and 2-(2-hydroxypropan-2-yl)benzofuran-5-carbaldehyde. Fomannosin and its precursors were identified in H. annosum 45-2. It was shown that the composition of the fermentation medium affected the number and range of the synthesized metabolites. Under in vitro conditions, all H. annosum and H. abietinum strains exhibited phytopathogenic effects on Pinus sylvestris L. seedlings, causing necrotic damage to stems of various degrees and plant death. Higher phytopathogenicity is characteristic of H. annosum strains: maximal aggressiveness was observed in H. annosum 45-2 with the greatest diversity of compounds in the metabolome profile and active fomannoxin production.

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Держатели документа:
Russian Acad Sci, Pushchino Sci Ctr, Biol Res Fed Res Ctr, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Forest Inst, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660049, Russia.
RAS, SB, FRC Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Antipova, T. V.; Zhelifonova, V. P.; Litovka, Yu. A.; Pavlov, I. N.; Baskunov, B. P.; Timofeev, A. A.; Kozlovsky, A. G.; Timofeev, Anton

/ T. V. Antipova, V. P. Zhelifonova, Y. A. Litovka [et al.] // iForest. - 2022. - Vol. 15. - P38-46, DOI 10.103832/ifor3840014. - Cited References:28 . - ISSN 1971-7458РУБ Forestry

Аннотация: Basidiomycetes Armillaria infect deciduous, coniferous and fruit trees, causing enormous economic damage. The role of secondary metabolites (tricyclic sesquiterpene aryl esters - melleolides) in the life cycle and pathogenesis of Armillaria is under active investigation. To date, not all species of Armillaria have been tested for the biosynthesis of melleolides. We investigated the secondary metabolite profiles of six root-pathogenic species of the genus Armillaria (A. borealis Marxmuller & Korhonen, A. cepistipes Velenovsky, A. gallica Marxm, A. mellea (Vahl) P. Kummer, A. sinapina Berube & Dessur, A. ostoyae (Romagn.) Herink) distributed in Siberia (South Krasnoyarsk Krai, Republic of Tyva, Republic of Khakassia, Taimyr Peninsula), Russian Far East (Sikhote-Alin) and Crimea (Krymsky National Park, Chatyr-Dag Mountain Lower Plateau). A total of 15 compounds were identified in the metabolome profile. Two compounds (melleolide D and melledonal C) are synthesized by all investigated strains irrespective of their geographic location and host plant. The maximum spectrum of melleolides (7-8 compounds) was found in isolates of A. borealis, A. gallica, A. sinapina, A. ostoyae. In submerged culture, the maximum accumulation of melleolides varied from 2 up to 239 mg l(-1). A mixture of melleolide D and melledonal C (1:1) synthesized by the most productive strain A. mellea Cr2-17 was first found to have a phytotoxic action on the growth parameters of the callus culture Populus balsamifera and 10-day-old conifer seedlings. A 0.5% concentration of melleolides caused a credible decrease of P. balsamifera callus raw biomass; a decrease of the viability of Larix sibirica and, which is especially significant, Pinus sylvestris seedlings; inhibition of stem and root growth processes; dechromation of foliage; loss of turgor. The occurrence of a broad range of melleolides in the metabolome profile and two common compounds in all investigated strains, with a phytotoxic action at their sufficiently high concentration, enables considering the synthesis of melleolides by Armillaria fungi as one of the possible mechanisms of their pathogenicity efficiently realized in strains characterized by overproduction of melleolides under natural conditions.

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Держатели документа:
Russian Acad Sci, GK Skryabin Inst Biochem & Physiol Microorganisms, FRC Pushchino Ctr Biol Res, 5 Prosp Nauki, Pushchino 142290, Moscow Region, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, FRC KSC, Siberian Branch, 50 Akad Gorodok Str, Krasnoyarsk 660036, Russia.
FSBEIHE MF Reshetnev Siberian State Univ Sci & Te, 82 Prosp Mira, Krasnoyarsk 660037, Russia.
FSBEIHE Krasnoyarsk State Agr Univ, 90 Prosp Mira, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Antipova, Tatyana, V; Zhelifonova, Valentina P.; Litovka, Yulia A.; Pavlov, Igor N.; Baskunov, Boris P.; Kokh, Zhanna A.; Makolova, Polina, V; Timofeev, Anton A.; Kozlovsky, Anatoly G.