Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / O. V. Kvitko, E. N. Muratova, E. V. Bazhina // Contemp. Probl. Ecol. - 2011. - Vol. 4, Is. 6. - P641-646, DOI 10.1134/S1995425511060129. - Cited References: 25. - The study has been carried out under support of the Russian Foundation of Basic Research (grants no. 08-04-90001Bel_a, 09-04-98000). . - 6. - ISSN 1995-4255РУБ Ecology

Аннотация: The cytogenetic study of Abies sibirica seed progenies in declining fir stands of West Sayan High Mountains has been carried out. The increased amount of chromosome and genome mutations, pathologies of mitotic cycle and cells with micronuclei were found at studying. The revealed irregularities may be the result of a long-term exposure to extreme environmental factors and probably reflect the high degree of disturbance of the ecosystem in this region.

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Держатели документа:
[Kvitko, O. V.
Muratova, E. N.
Bazhina, E. V.] Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Kvitko, O.V.; Muratova, E.N.; Bazhina, E.V.

/ O. V. Goryachkina [et al.] // Cytologia. - 2018. - Vol. 83, Is. 3. - P322-328, DOI 10.1508/cytologia.83.323. - Cited References:34. - Authors express sincerely thanks to Dr. Oreshkova N.V., V. N. Sukachev Institute of Forest SB RAS, for supplying of L. sibirica genomic DNA, and Dr. Shcherban A.B., The Federal Research Center Institute of Cytology and Genetics SB RAS for preparation of 5S rDNA probe. The study was funded by Russian Foundation for Basic Research and Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support (project No 16-44-243068), Russian Foundation for Basic Research and Belarus (project 18-54-00010), Investment Foundation FASIE (project 0040522). . - ISSN 0011-4545РУБ Cell Biology + Genetics & Heredity

Аннотация: We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91-96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica.

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Russian Acad Sci, Sukachev Inst Forest, Krasnoyarsk Sci Ctr, Siberian Branch,Fed Res Ctr, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Vavilov Inst Gen Genet, Gubkin Str 3, Moscow 119333, Russia.

Доп.точки доступа:
Goryachkina, Olga V.; Park, Maria E.; Tretyakova, Iraida N.; Badaeva, Ekaterina D.; Muratova, Elena N.; Russian Foundation for Basic Research; Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support [16-44-243068]; Investment Foundation FASIE [0040522]; [18-54-00010]

/ A. Bolsunovsky [et al.] // J. Environ. Radioact. - 2019. - Vol. 207. - P1-6, DOI 10.1016/j.jenvrad.2019.05.014 . - ISSN 0265-931X
Аннотация: The Allium-test is commonly used to assess genotoxicity of chemical and physical factors. In the present study, the roots of germinating onion (Allium cepa) were exposed to 0.02–13 Gy of ?-radiation. The dose dependencies of the frequency of chromosomal aberrations and micronuclei were nonlinear. An increase in the frequency of chromosomal aberrations in germinating seed root cells was first found under exposure to low doses of ?-radiation (0.05 and 0.1 Gy). Micronuclei inductions at low doses of radiation were not significantly different from the control. Our study suggests that germinating onion seed roots are a sensitive bioassay material for assessing the genotoxic effects of low-dose ?-radiation. © 2019 Elsevier Ltd

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Institute of Biophysics Siberian Branch of Russian Academy of Sciences, 50-50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Forest Siberian Branch of Russian Academy of Sciences, 50-28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Budker Institute of Nuclear Physics of the Siberian Branch of the Russian Academy of Sciences, 11 Lavrentyev Ave., Novosibirsk, 630090, Russian Federation

Доп.точки доступа:
Bolsunovsky, A.; Dementyev, D.; Trofimova, E.; Iniatkina, E.; Kladko, Y.; Petrichenkov, M.