Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ A. N. Kravchenko, A. K. Ekart, A. Y. Larionova // Russ. J. Gen. - 2016. - Vol. 52, Is. 11. - P1142-1148, DOI 10.1134/S1022795416090088 . - ISSN 1022-7954
Аннотация: The results of the study of 21 populations of Siberian spruce (Picea obovata Ledeb.) from different parts of the species natural range by microsatellite (SSR) analysis of nuclear DNA are presented. Using nine loci developed for Picea abies (L.) Karst. and Picea glauca (Moench) Voss and detecting variation in Picea obovata, the parameters of intra- and interpopulation genetic diversity, as well as the degree of population differentiation, were determined. It was demonstrated that the population of Siberian spruce in the study was characterized by a relatively high average level of intrapopulation variability (Ho = 0.408; He = 0.423) and low interpopulation differentiation (Fst = 0.048, P = 0.001) at this class of DNA markers. The genetic distance between populations ranged from 0.009 to 0.167, averaging 0.039. The isolated Magadan population, located in the extreme Northeast of Russia at a considerable distance from the main species range and characterized by the lowest genetic diversity among the studied populations, was maximally differentiated from the rest of the spruce populations. In addition, the steppe Ubukun population from Buryatia and the population from the Bogd Khan Uul Biosphere Reserve, Mongolia, were considerably different in the genetic structure from most populations of Siberian spruce, although to a lesser extent than the Magadan population. These findings are consistent with the results of previous studies of this species carried out using allozyme and microsatellite loci of chloroplast DNA and point to the prospects of using nuclear microsatellites as DNA markers to analyze the population genetic structure of Siberian spruce. © 2016, Pleiades Publishing, Inc.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kravchenko, A. N.; Ekart, A. K.; Larionova, A. Y.

/ M. A. Polezhaeva [et al.] // Russ. J. Genet. - 2019. - Vol. 55, Is. 6. - P762-766, DOI 10.1134/S1022795419060115. - Cited References:28. - This work (population collection) was carried out within the framework of the government order of the Research Institute of Ecology of Plants and Animals of the Ural Branch of the Russian Academy of Sciences. The molecular genetic analysis was supported by the Russian Foundation for Basic Research, grant no. 15-04-01640. . - ISSN 1022-7954. - ISSN 1608-3369РУБ Genetics & Heredity

Аннотация: SSR analysis of Rhododendron aureum Georgi in eight populations (63 samples) from Siberia (West and East Sayans) and Kamchatka was performed. Polymorphism of five SSR loci showed a high level of genetic diversity in all the populations (mean values H-o = 0.667, H-e = 0.638). The analysis of molecular variation (AMOVA) revealed that about 16% of the genetic diversity of R. aureum occurs in interpopulation differences (F-st = 0.161, p = 0.001). The Bayesian approach in the STRUCTURE program revealed that the species has a heterogeneous genetic structure-populations from Kamchatka form a separate cluster and show high homogeneity, and populations from Western and Eastern Siberia are of mixed origin. The obtained results indicate the prospects of using this type of markers for population-genetic studies of R. aureum.

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Держатели документа:
Russian Acad Sci, Inst Plant & Anim Ecol, Ural Branch, Ekaterinburg 620144, Russia.
Russian Acad Sci, Inst Biol Problems North, Far East Branch, Magadan 685000, Russia.
Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Polezhaeva, M. A.; Tikhonova, N. A.; Modorov, M., V; Ranyuk, M. N.; Korchagina, O. S.; Russian Foundation for Basic Research [15-04-01640]

/ N. V. Oreshkova, T. S. Sedel’nikova, S. P. Efremov, A. V. Pimenov // Contemp. Probl. Ecol. - 2020. - Vol. 13, Is. 6. - P569-576, DOI 10.1134/S1995425520060116 . - ISSN 1995-4255
Аннотация: Abstract: The DNA polymorphism of seven coenopopulations of Siberian stone pine (Pinus sibirica Du Tour) growing in a region which is typical for mountain taiga pine forests—the northeastern part of the Kuznetsk Alatau—has been studied. According to the data of 11 nuclear microsatellite loci, 44 allelic variants which significantly differed between the studied coenopopulations in composition and frequency of occurrence are identified. The highest level of allelic diversity is found in the Ps_80612 and Ps_1502048 loci, in which eight and seven alleles are detected, respectively. The calculation of the main parameters of genetic diversity have shown, on the whole, a relatively low level of polymorphism of the studied samples (NA = 3.078, NE = 1.877, HE = 0.445, and HO = 0.401). An analysis of the degree of subdivision of coenopopulations has revealed that about 95% of the total genetic diversity is concentrated within populations, while the interpopulation component accounts for only about 5% (FST = 0.049). The diagnosed differences in the level of P. sibirica genetic polymorphism are determined by several factors: the presence of orographic and phytocenotic barriers between the coenopopulations, which define the directions of seed propagation vectors along the watercourses of river basins that form the Chulym River in the eastern sector of the Kuznetsk Alatau and Tom River in the western sector; a significant level of bioclimatic and environmental extremeness of habitats of coenopopulations in accordance with the height above sea level; and anthropogenic pressure, including periodic deforestation and recreational impact on coenopopulations located near settlements. These factors, which have a significant microevolutionary component, determine the adaptation trends of coenopopulations of P. sibirica and morphological singularities and reproductive potential of trees and, ultimately, they control their genetic polymorphism in the studied part of the Kuznetsk Alatau. © 2020, Pleiades Publishing, Ltd.

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Держатели документа:
Federal Research Center, Kola Scientific Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Oreshkova, N. V.; Sedel’nikova, T. S.; Efremov, S. P.; Pimenov, A. V.

/ N. V. Oreshkova, T. S. Sedel'nikova, S. P. Efremov, A. V. Pimenov // Contemp. Probl. Ecol. - 2020. - Vol. 13, Is. 6. - P569-576, DOI 10.1134/S1995425520060116. - Cited References:28 . - ISSN 1995-4255. - ISSN 1995-4263РУБ Ecology

Аннотация: The DNA polymorphism of seven coenopopulations of Siberian stone pine (Pinus sibirica Du Tour) growing in a region which is typical for mountain taiga pine forests-the northeastern part of the Kuznetsk Alatau-has been studied. According to the data of 11 nuclear microsatellite loci, 44 allelic variants which significantly differed between the studied coenopopulations in composition and frequency of occurrence are identified. The highest level of allelic diversity is found in the Ps_80612 and Ps_1502048 loci, in which eight and seven alleles are detected, respectively. The calculation of the main parameters of genetic diversity have shown, on the whole, a relatively low level of polymorphism of the studied samples (N-A = 3.078, N-E = 1.877, H-E = 0.445, and H-O = 0.401). An analysis of the degree of subdivision of coenopopulations has revealed that about 95% of the total genetic diversity is concentrated within populations, while the interpopulation component accounts for only about 5% (F-ST = 0.049). The diagnosed differences in the level of P. sibirica genetic polymorphism are determined by several factors: the presence of orographic and phytocenotic barriers between the coenopopulations, which define the directions of seed propagation vectors along the watercourses of river basins that form the Chulym River in the eastern sector of the Kuznetsk Alatau and Tom River in the western sector; a significant level of bioclimatic and environmental extremeness of habitats of coenopopulations in accordance with the height above sea level; and anthropogenic pressure, including periodic deforestation and recreational impact on coenopopulations located near settlements. These factors, which have a significant microevolutionary component, determine the adaptation trends of coenopopulations of P. sibirica and morphological singularities and reproductive potential of trees and, ultimately, they control their genetic polymorphism in the studied part of the Kuznetsk Alatau.

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Держатели документа:
Russian Acad Sci, Fed Res Ctr, Kola Sci Ctr, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Oreshkova, N., V; Sedel'nikova, T. S.; Efremov, S. P.; Pimenov, A., V

/ M. A. Polezhaeva, N. A. Tikhonova, E. A. Marchuk [et al.] // J. Plant Res. - 2021, DOI 10.1007/s10265-020-01241-9 . - Article in press. - ISSN 0918-9440
Аннотация: The vast territory of East Asia, including southwestern Beringia, is considered to have been almost ice free during the Pleistocene. Cold-resistant flora may have persisted in this region expanding or contracting its range during the climate cooling. Only a few plant genera have been studied with a sampling area across their entire geographic range in East Asia; therefore, the understanding of the biogeographic history of alpine flora in this region remains limited. In the present study, genetic variation and population structure in 21 populations of the alpine shrub Rhododendron aureum across its range in East Asia were assessed using 18 microsatellite loci. Phylogenetic analyses revealed three main genetic groups: Siberia, Northeast, and North Pacific. According to the geographical pattern of genetic diversity, the North Pacific group includes populations from Kamchatka, south of Russian Far East, and territories close to central Japan. This group is the most diverse and likely diverged earlier than the Siberia and Northeast groups. Ecological niche modeling predicts range expansion of this species during the period of cooling and, together with demographic history, suggests that the divergence between the three main genetic groups predated the Last Glacial Maximum. Similar to other cold-resistant species such as Larix sibirica and Juniperus communis, the pattern of genetic diversity of R. aureum supports the survival of the species at high latitudes during the Pleistocene with limited contribution of the southern populations to expansion of the species range to the Northeast region and Siberia. © 2021, The Botanical Society of Japan.

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Держатели документа:
Institute of Plant and Animal Ecology, Ural Branch of the Russian Academy of Sciences, Ekaterinburg, Russian Federation
V. N. Sukachev Institute of Forest, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Botanical Garden-Institute, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russian Federation
Institute of the Biological Problems of the North, Far Eastern Branch of the Russian Academy of Sciences, Magadan, Russian Federation
Institute of General and Experimental Biology, Siberian Branch of the Russian Academy of Sciences, Ulan-Ude, Russian Federation

Доп.точки доступа:
Polezhaeva, M. A.; Tikhonova, N. A.; Marchuk, E. A.; Modorov, M. V.; Ranyuk, M. N.; Polezhaev, A. N.; Badmayeva, N. K.; Semerikov, V. L.

/ M. A. Polezhaeva, N. A. Tikhonova, E. A. Marchuk [et al.] // J. Plant Res. - 2021, DOI 10.1007/s10265-020-01241-9. - Cited References:77. - We are grateful to A. Berkutenko, D. Krivenko, A. Shirayev, L. Andriyanova, M. Khoreva, P. Krestov, T. Polyakova, A. Efimova, N. Molokova for the help with material collections. We thank V. Mikryukov for the help with MAXENT figures presentation. We also thank two anonymous reviewers for helpful comments on the manuscript. The collection of samples was supported by the State Contract of the Institute of Plant and Animal Ecology, UB RAS. The laboratory treatments were supported by the Russian Science Foundation for Basic Research (Project No. 20-04-00417 A). . - Article in press. - ISSN 0918-9440. - ISSN 1618-0860РУБ Plant Sciences

Аннотация: The vast territory of East Asia, including southwestern Beringia, is considered to have been almost ice free during the Pleistocene. Cold-resistant flora may have persisted in this region expanding or contracting its range during the climate cooling. Only a few plant genera have been studied with a sampling area across their entire geographic range in East Asia; therefore, the understanding of the biogeographic history of alpine flora in this region remains limited. In the present study, genetic variation and population structure in 21 populations of the alpine shrub Rhododendron aureum across its range in East Asia were assessed using 18 microsatellite loci. Phylogenetic analyses revealed three main genetic groups: Siberia, Northeast, and North Pacific. According to the geographical pattern of genetic diversity, the North Pacific group includes populations from Kamchatka, south of Russian Far East, and territories close to central Japan. This group is the most diverse and likely diverged earlier than the Siberia and Northeast groups. Ecological niche modeling predicts range expansion of this species during the period of cooling and, together with demographic history, suggests that the divergence between the three main genetic groups predated the Last Glacial Maximum. Similar to other cold-resistant species such as Larix sibirica and Juniperus communis, the pattern of genetic diversity of R. aureum supports the survival of the species at high latitudes during the Pleistocene with limited contribution of the southern populations to expansion of the species range to the Northeast region and Siberia.

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Держатели документа:
Russian Acad Sci, Inst Plant & Anim Ecol, Ural Branch, Ekaterinburg, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk, Russia.
Russian Acad Sci, Bot Garden Inst, Far Eastern Branch, Vladivostok, Russia.
Russian Acad Sci, Inst Biol Problems North, Far Eastern Branch, Magadan, Russia.
Russian Acad Sci, Inst Gen & Expt Biol, Siberian Branch, Ulan Ude, Russia.

Доп.точки доступа:
Polezhaeva, Maria A.; Tikhonova, Natalya A.; Marchuk, Elena A.; Modorov, Makar, V; Ranyuk, Maryana N.; Polezhaev, Alexey N.; Badmayeva, Natalya K.; Semerikov, Vladimir L.; State Contract of the Institute of Plant and Animal Ecology, UB RAS; Russian Science Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00417 A]

[Текст] : статья / Наталья Викторовна Орешкова, Тамара Станиславовна Седельникова, Станислав Петрович Ефремов, Александр Владимирович Пименов // Сибирский экологический журнал. - 2020. - Т. 27, № 6. - С. 677-688 . - ISSN 0869-8619

УДК

575.174.015.3

582.475.2

Аннотация: Исследован полиморфизм ДНК семи ценопопуляций сосны сибирской кедровой (Pinus sibirica Du Tour), произрастающих в характерном для горно-таежных кедровников регионе - северо-восточной части Кузнецкого Алатау. По 11 ядерным микросателлитным локусам выявлено 44 аллельных варианта, существенно отличающихся у изученных ценопопуляций по составу и частотам встречаемости. Самый высокий уровень аллельного разнообразия имеют локусы Ps _80612, Ps _1502048, в которых выявлено 8 и 7 аллелей соответственно. Расчет основных параметров генетического разнообразия показал в целом сравнительно невысокий уровень полиморфизма исследованных выборок ( NA = 3,078; NE = 1,877; HE = 0,445; HO = 0,401). Анализ степени подразделенности ценопопуляций выявил, что внутри популяций сосредоточено около 95 % всего генетического разнообразия, тогда как на межпопуляционную составляющую приходится всего около 5 % (F ST = 0,049). Диагностированные различия в уровне генетического полиморфизма P. sibirica определяются: наличием между ценопопуляциями орографических и фитоценотических барьеров, обусловливающих направления векторов распространения семян вдоль водотоков бассейнов рек, формирующих р. Чулым в восточном секторе Кузнецкого Алатау и р. Томь - в западном; значительным уровнем биоклиматической и экологической экстремальности местопроизрастаний ценопопуляций в соответствии с высотой над уровнем моря; антропогенной нагрузкой, включающей периодические рубки и рекреационное воздействие на расположенные вблизи населенных пунктов ценопопуляции. Данные факторы, имеющие выраженную микроэволюционную составляющую, детерминируют адаптивные тренды ценопопуляций P. sibirica, морфологическое своеобразие и репродуктивный потенциал деревьев и, в конечном счете, контролируют их генетический полиморфизм в исследованной части Кузнецкого Алатау.
The DNA polymorphism of 7 coenopopulations of Siberian stone pine ( Pinus sibirica Du Tour), growing in the region which is typical for mountain taiga pine forests - the north-eastern part of the Kuznetsk Alatau, was studied. According to the data of 11 nuclear microsatellite loci, 44 allelic variants, which significantly differed between the studied cenopopulations in composition and frequency of occurrence were identified. The highest level of allelic diversity is found in the Ps_80612, Ps_1502048 loci, in which 8 and 7 alleles were detected, respectively. The calculation of the main parameters of genetic diversity showed, on the whole, a relatively low level of polymorphism of the studied samples ( NA = 3,078; NE = 1,877; HE = 0,445; HO = 0,401). An analysis of the degree of subdivision of cenopopulations revealed that about 95 % of the total genetic diversity is concentrated within populations, while the interpopulation component accounts for only about 5 % ( FST = 0,049). The diagnosed differences in the level of P. sibirica genetic polymorphism are determined by several factors: the presence of orographic and phytocenotic barriers between the coenopopulations which defined the directions of seed propagation vectors along the watercourses of river basins that formed the Chulym river in the eastern sector of the Kuznetsk Alatau and Tom river in the western sector; a significant level of bioclimatic and environmental extremeness of habitats of coenopopulations in accordance with the height above sea level; anthropogenic pressure, including periodic deforestation and recreational impact on coenopopulations located near settlements. These factors, which have a significant microevolutionary component, determine the adaptation trends of cenopopulations of P. sibirica , morphological singularities and reproductive potential of trees and, ultimately, control their genetic polymorphism in the studied part of the Kuznetsk Alatau

РИНЦ

Держатели документа:
Институт леса им. В. Н. Сукачева СО РАН
Сибирский федеральный университет
ФИЦ КНЦ СО РАН

Доп.точки доступа:
Орешкова, Наталья Викторовна; Седельникова, Тамара Станиславовна; Ефремов, Станислав Петрович; Пименов, Александр Владимирович