Кл.слова (ненормированные):
Lab on a chip -- Mollisol -- Oxisol -- Soil biogeochemical interface -- SoilChip -- X-ray photoelectron spectroscopy -- Biogeochemistry -- Biological materials -- Dynamics -- Lab-on-a-chip -- Microorganisms -- Photoelectrons -- Photons -- Soils -- X ray photoelectron spectroscopy -- Biogeochemical interfaces -- Dissolved organic matters -- Ecological process -- Integrated techniques -- Mollisols -- Oxisol -- Soil microenvironments -- SoilChip -- Underwater soils
Аннотация: Many soil functions are modulated by processes at soil biogeochemical interfaces (BGIs). However, characterizing the elemental dynamics at BGIs is hampered by the heterogeneity of soil microenvironments. In order to investigate the processes of BGI formation in an upland soil (Mollisol) and a paddy soil (Oxisol), we developed a SoilChip method by assembling dispersed soil particles onto homogeneous 800-?m-diameter microarray chips and then submerging them in a solution that contained dissolved organic matter (OM) extracted from one of the two soils. The chips with Mollisol particles were incubated at 95–100% humidity, whereas the chips with Oxisol particles were incubated at 100% humidity. Dynamics of individual elements at the soils’ BGIs were quantitatively determined using X-ray photoelectron spectroscopy (XPS). Distinct differences in the soil-microbe complexes and elemental dynamics between the Mollisol and Oxisol BGIs suggested that the formation of specific BGIs resulted from the complex interaction of physical, chemical, and microbial processes. By integrating the SoilChip and XPS, it was possible to elucidate the dynamic formation of the two different soil BGIs under standardized conditions. Therefore, the SoilChip method is a promising tool for investigating micro-ecological processes in soil. © 2017
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Держатели документа:
Key Laboratory of Agro-ecological Processes in the Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, China
University of Chinese Academy of Sciences, Beijing, China
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics – Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
Institute of Soil Science, Leibniz Universitat Hannover, Hannover, Germany
VN Sukachev Institute of Forest, Russian Academy of Sciences - Siberian Branch, Akademgorodok, Krasnoyarsk, Russian Federation
Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze River), Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University, Wuhan, China
Доп.точки доступа:
Huang, X.; Li, Y.; Liu, B.; Guggenberger, G.; Shibistova, O.; Zhu, Z.; Ge, T.; Tan, W.; Wu, J.