Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / G. G. Polyakova, V. V. Stasova, N. V. Pashenova // Russ. J. Plant Physiol. - 2011. - Vol. 58, Is. 5. - P819-827, DOI 10.1134/S1021443711050177. - Cited References: 23. - This work was supported by the Russian Foundation for Basic Research, project no. 09-04-09030. . - 9. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Ophiostomatoid fungi colonize the conducting tissues of conifer stems, the phloem and the xylem. These pathogenic fungi penetrate into the stem through injuries made by xylophagous insects vectoring these pathogens. In this study the response of the phloem of Scotch pine (Pinus sylvestris L.) to wounding (treatment 1) was compared with the response to wounding combined with application of high-molecular-weight compounds isolated from the mycelium of the ophiostomatoid fungus Ceratocystis laricicola Redfern & Minter (treatment 2). Both treatments induced the appearance of necrosis in the inner bark, the formation of periderm separating living and dead tissues, and formation of the callus alongside the wound perimeter. In addition, the bark accumulated lignin, bound proanthocyanidins, and resins, with a parallel decrease in the content of free proanthocyanidins, low-molecular-weight carbohydrates, and non-lignin components of the cell wall (P > 0.95). The size of necrotic spots, as well as changes in the content of most substances, were significantly higher in the treatment 2 than in the treatment 1 (P > 0.95). The accumulation of lignin in cell walls of phloem sieve cells was delayed in the treatment 2 as compared with that in the treatment 1. This suggested that the mycelial extract temporarily inhibited lignification at the early stage of the wound response. This disturbance of the cell wall protective transformation led to the hypothesis that the fungal suppressors retard the repair of inner bark injured by insects, thereby favoring the invasion of conifer tissues by ophiostomatoid fungi.

Полный текст,
WOS,
Scopus

Держатели документа:
[Polyakova, G. G.
Stasova, V. V.
Pashenova, N. V.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Stasova, V.V.; Pashenova, N.V.

[Text] / G. G. Polyakova [et al.] // Russ. J. Plant Physiol. - 2008. - Vol. 55, Is. 4. - P496-502, DOI 10.1134/S1021443708040109. - Cited References: 25 . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: The elicitor activity of compounds extracted from the mycelia of six species of phytopathogenic fungi was assessed from the sizes of necrotic lesions on the external surface of the living trunk phloem of five coniferous species inhabiting Siberia: Siberian larch (Larix sibirica L.), Scotch pine (Pinus sylvestris L.), Siberian spruce (Picea obovata Ledeb.), Siberian fir (Abies sibirica L.), and cedar pine (Pinus sibirica (Rupr.) Mayr.). The compounds for inoculation were extracted from the mycelium of ascomycetes imperfect, and basidium fungi; the living mycelia of these fungi were also used. The fungal extract or mycelium was placed into the hollows 7 mm in diameter in the trunk bark. Infection triggered the formation of hypersensitivity necrotic lesions in the inner bark exceeding in size those appeared after control wounding of four tree species (larch, pine, spruce, and cedar); fir was an exclusion. In experiments with tree trunks and conifer calluses, a dependence of immune response parameters (the sizes of necrotic lesions and the content of lignin and bound proanthocyanidins) on the quantity of the fungal preparation was elucidated. The largest necrotic lesions appeared after injection of 500 mu g of the fungal preparation into the hollow in the trunk, and its higher quantities did not increase the indices measured. The size of the necrotic lesion on the trunk bark is supposed to be used as a promising index characterizing the level of tree immunity and tolerance under various ecological conditions.

Полный текст,
WOS,
Scopus

Держатели документа:
[Polyakova, G. G.
Pashenova, N. V.
Polyakov, V. I.
Zrazhevskaya, G. K.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Pashenova, N.V.; Polyakov, V.I.; Zrazhevskaya, G.K.

[Text] / I. V. Shein [et al.] // Russ. J. Plant Physiol. - 2003. - Vol. 50, Is. 4. - P516-521, DOI 10.1023/A:1024776924788. - Cited References: 24 . - 6. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Twelve-day-old seedlings of Scots pine (Pinus sylvestris L.) were infected with a virulent spore suspension of the fungus Fusarium sporotrichiella var. sporotrichioides Bilai. 72 h after infection, the seedlings were divided into infection-susceptible and infection-resistant ones, and phenolic compounds in the seedling hypocotyls were analyzed. Susceptible seedlings contained 4.3-fold less lignins, 1.4-fold less free and bound proanthocyanidins, 12.7-fold less free hydroxybenzoic acids, 56.6-fold less bound hydroxybenzoic acids, 2-fold less quinic acid, and 5.1-fold less shikimic acid as compared to the resistant seedlings. At the same time, L-phenylalanine content was increased 3-fold and that of L-tyrosine, 1.3-fold. The activity of quinate:NAD(P)(+) oxidoreductase and L-phenylalanine ammonia-lyase in the hypocotyls of susceptible seedlings was 2.3-fold and 1.4-fold lower, respectively, whereas the activity of shikimate:NAD(P)(+) oxidoreductase remained unchanged.

Полный текст,
WOS,
Scopus

Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Shein, I.V.; Shibistova, O.B.; Zrazhevskaya, G.K.; Astrakhantseva, N.G.; Polyakova, G.G.

[Text] / I. V. Shein [et al.] // Russ. J. Plant Physiol. - 2001. - Vol. 48, Is. 2. - P216-221, DOI 10.1023/A:1009056201926. - Cited References: 23 . - 6. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Callus cultures of Siberian larch (Lar ix sibirica Ledeb.) and Siberian spruce (Picea obovata Ledeb.) were used to demonstrate the elicitor activity of two ophiostomatoid fungal species, Ceratocystis laricicola and Ceratocystis polonica, as the pioneer settlers on larch and spruce, respectively. The extract from C. laricicola mycelium stimulated the accumulation of lignin in larch cells by 37% and that of hound proanthocyanidins by 25%. In spruce callus cultures, C. laricicola and C. polonica increased the bound PA content by 25 and 46%, respectively. In the callus cultures of larch and spruce, the addition of extract of C. laricicola increased the concentration of p-hydroxybenzoic acid 13-fold and nearly 4-fold, respectively. The metabolic characteristics of accumulation of phenolic compounds in conifer cells are discussed.

Полный текст,
WOS,
Scopus

Держатели документа:
Russian Acad Sci, Sukachev Forest Inst, Siberian Div, Akademgorodok 660036, Krasnoyarsk, Russia

Доп.точки доступа:
Shein, I.V.; Polyakova, G.G.; Zrazhevskaya, G.K.; Pashenova, N.V.; Vetrova, V.P.

[Text] / G. G. POLYAKOVA [et al.] // Russ. J. Plant Physiol. - 1995. - Vol. 42, Is. 4. - P552-557. - Cited References: 21 . - 6. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: The rate of accumulation and location of proanthocyanidins and lignin was studied in fir (Abies sibirica Ledeb.) trunk phloem inoculated with two species of pathogenic micromycetes Verticillium sp. and Thysanophora sp., the usual associates of the sawyer Monochamus urussovi Fisch. Greater accumulation of the phenolic substances and more rapid localization of pathogen as a result of a hypersensitivity reaction were detected in firs infected with Verticillium sp. It was concluded that accumulation of proanthocyanidins and lignin is a part of nonspecific immune response of fir to the pathogen action and that the pathogen specific of fir can inhibit induced synthesis of defensive substances by the host plant.


Доп.точки доступа:
POLYAKOVA, G.G.; VETROVA, V.P.; PASHENOVA, N.V.; OSIPOV, V.I.

/ G. G. Polyakova, V. A. Senashova // Mikol. Fitopatol. - 2017. - Vol. 51, Is. 3. - С. 168-177 . - ISSN 0026-3648
Аннотация: Biotrophic fungus Melampsorella caryophyllacearum (Pucciniastraceae, Pucciniomycetes, Basidiomycota) developing in tissues of a fir (Abies sibirica) causes broom rust. Physiological mechanisms of plant resistance and fungus pathogenicity are not clear. The purpose of this work was an assessment of a role of secondary compounds and carbohydrates in mechanisms of interaction of Siberian fir and fungus M. caryophyllacearum. The 20-year age trees of Siberian fir grow in a mountain taiga zone in suburban forests of Krasnoyarsk (Central Siberia). Two options (one-year axes and needles of the current year without signs of damage by the fungus, and infected ones) were compared. Samples were taken on 3 trees 3 times during vegetation season: on June 21 (a phenology stage of shoot growth and a formation of pathogen etion), on July 19 (a phenology stage of summer vegetation and the period of active sporulation) and on September 10 (a phenology stage of autumn coloration of leaves and dying off the infected needles). The lignin and resin content were determined by weight method (the lignin determined with thioglycolic acid, the resins - by dissolving in pentane), carbohydrates - by the method of copper reduction using glucose as a standard, PAs - by coloring of solution after adding n-butanol / HCl mixture and heating. Starch was determined by coloring with iodine. The analysis of ANOVA proved reliable influence of various factors (phenology stage, plant organ (needles, axis), presence/absence of fungus infestation) on the content of PAs, lignin, monosaccharides (p < 0.05). The content of resin depended on plant organ only (resin content was more in axes, than in needles; p < 0.05). The tendency to starch accumulation in the infected axes during vegetation appeared to be caused by breakage of carbohydrates outflow. Infecting by the fungus caused decrease in the content of monosaccharides (p<0.05) that probably was connected with active consumption of mobile carbohydrates by the biotroph. The accumulation of PAs was revealed in needles and axes induced by M. caryophyllacearum fungus. Unlike PAs, the lignin concentration differently changed in the infected plant tissues. The accumulation of lignin was noted in diseased needles. In the infected axes in July the lignin content was significantly lower in comparison with control (p < 0.05). The found effect appeared to be manifestation of successful inhibition of plant protection by fungus. We suggested a hypothesis of the delayed lignification according to which a fungus inhibits synthesis of lignin and increases the chances in overcoming of protective barriers of a host. Perhaps, the pathogen influences carbon distribution by reducing its part for synthesis of lignin and increasing thereby synthesis of carbohydrates which further actively uses. The obtained data is agreed with concept according to which with effector molecules, biotrophs manipulate the defense machinery of the host in order to delay defense responses to gain enough time to multiply and spread into neighboring cells. © 2017 Russian Academy of Sciences. All rights reserved.

Scopus

Держатели документа:
Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Polyakova, G. G.; Senashova, V. A.

[Текст] : статья / Г. Г. Полякова, Н. В. Пашенова, В. А. Сенашова // Известия РАН. Серия Биологическая. - 2020. - № 1. - С. 44-50, DOI 10.31857/S0002332920010105 . - ISSN 0002-3329
   Перевод заглавия: Influence of Chronical Partial Defoliation of Larch by Casebeareron Stem Physiological Parameters

УДК	581.1:632.938

Аннотация: В очаге лиственничной чехликовой моли Coleophora sibiricella Falkovitsh в чистом лиственничнике исследован защитный ответ флоэмы ствола лиственницы сибирской Larix sibirica Ledeb. на поранение или инокуляцию ствола грибным индуктором. В качестве грибного инокулята использовали мицелий офиостомового гриба Ceratocystis laricicola Redfern & Minter, его экстракт и белковую фракцию, полученную из экстракта. Содержание лигнина, крахмала, конденсированных дубильных веществ (проантоцианидинов) во флоэме определено в начале опыта (0 сут), через 7 и 14 сут. С помощью анализа ANOVA показано, что параметры ответа флоэмы не зависели от формы грибного инокулята (р ≥ 0.918), но зависели от других факторов (р ≤ 0.005) – повреждения кроны молью, времени от начала опыта, наличия инокулята в ране
In the long-term explosion of mass reproduction of larch casebearer Coleophora sibiricella Falkovitsh in pure larch stand the stem phloem responses of larch trees (Larix sibirica Ledeb.) to wounding damage or inoculation of the trunk with the fungal inductor. Mycelium of the ophiostomoid fungus Ceratocystis laricicola Redfern & Minter, its extractive and protein fraction obtained from the extractive were used as a fungal inductor. The content of lignin, starch, condensed tannins (proanthocyanidins) in the phloem was determined at the beginning of the experiment (0 days), after 7 and 14 days. Using ANOVA analysis, it was shown that the response parameters of the phloem did not depend on the shape of the fungal inoculum (p ≥ 0.918), but depended on other factors (p ≤ 0.005) - damage to the crown by the moth, time from the start of the experiment, and the presence of the inoculum in the wound

РИНЦ

Держатели документа:
Институт леса им. В.Н. Сукачева СО РАН

Доп.точки доступа:
Полякова, Галина Геннадьевна; Polyakova Galina Gennad'yevna; Пашенова, Наталья Вениаминовна; Pashenova, Natal'ya Veniaminovna; Сенашова, Вера Александровна; Syenashova Vera Alexandrovna

/ G. G. Polyakova, N. V. Pashenova, V. A. Senashova // Biol. Bull. - 2020. - Vol. 47, Is. 1. - P49-55, DOI 10.1134/S1062359020010100. - Cited References:28. - This work was supported by the Russian Foundation for Basic Research, project no. 15-04-06575. . - ISSN 1062-3590. - ISSN 1608-3059РУБ Biology

Аннотация: In the long-term explosion of mass reproduction of the larch casebearer (Coleophora sibiricella Falkovitsh) in a pure larch stand, the stem phloem responses of larch trees (Larix sibirica Ledeb.) to wounding damage or inoculation of the trunk with the fungal inductor were studied. The mycelium of the ophiostomatoid fungus Ceratocystis laricicola Redfern & Minter and its extractive and the protein fraction obtained from the extractive were used as a fungal inductor. The content of lignin, starch, and condensed tannins (proanthocyanidins) in the phloem was determined at the beginning of the experiment (zero days), and after seven and 14 days. Using ANOVA analysis, it was shown that the response parameters of the phloem did not depend on the kind of the fungal inoculum (P = 0.918), but depended on other factors (P

WOS

Держатели документа:
Sukachev Inst Forest, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Polyakova, G. G.; Pashenova, N. V.; Senashova, V. A.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [15-04-06575]