Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ O. . Shibistova [et al.] // PLoS One. - 2012. - Vol. 7, Is. 9. - Ст. e45540, DOI 10.1371/journal.pone.0045540. - Cited References: 87. - Financial support was given by the German Research Foundation (to G. G., DFG Gu 406/19-1). The funding agency had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - 11. - ISSN 1932-6203РУБ Multidisciplinary Sciences

Аннотация: Background: Anthropogenic disturbance of old-growth tropical forests increases the abundance of early successional tree species at the cost of late successional ones. Quantifying differences in terms of carbon allocation and the proportion of recently fixed carbon in soil CO2 efflux is crucial for addressing the carbon footprint of creeping degradation. Methodology: We compared the carbon allocation pattern of the late successional gymnosperm Podocarpus falcatus (Thunb.) Mirb. and the early successional (gap filling) angiosperm Croton macrostachyus Hochst. es Del. in an Ethiopian Afromontane forest by whole tree (CO2)-C-13 pulse labeling. Over a one-year period we monitored the temporal resolution of the label in the foliage, the phloem sap, the arbuscular mycorrhiza, and in soil-derived CO2. Further, we quantified the overall losses of assimilated C-13 with soil CO2 efflux. Principal Findings: C-13 in leaves of C. macrostachyus declined more rapidly with a larger size of a fast pool (64% vs. 50% of the assimilated carbon), having a shorter mean residence time (14 h vs. 55 h) as in leaves of P. falcatus. Phloem sap velocity was about 4 times higher for C. macrostachyus. Likewise, the label appeared earlier in the arbuscular mycorrhiza of C. macrostachyus and in the soil CO2 efflux as in case of P. falcatus (24 h vs. 72 h). Within one year soil CO2 efflux amounted to a loss of 32% of assimilated carbon for the gap filling tree and to 15% for the late successional one. Conclusions: Our results showed clear differences in carbon allocation patterns between tree species, although we caution that this experiment was unreplicated. A shift in tree species composition of tropical montane forests (e. g., by degradation) accelerates carbon allocation belowground and increases respiratory carbon losses by the autotrophic community. If ongoing disturbance keeps early successional species in dominance, the larger allocation to fast cycling compartments may deplete soil organic carbon in the long run.

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Держатели документа:
[Shibistova, Olga
Yohannes, Yonas
Boy, Jens
Guggenberger, Georg] Leibniz Univ Hannover, Inst Soil Sci, Hannover, Germany
[Shibistova, Olga] Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forest, Krasnoyarsk, Russia
[Yohannes, Yonas] Ethiopian Inst Agr Res, Addis Ababa, Ethiopia
[Richter, Andreas
Wild, Birgit
Watzka, Margarethe] Univ Vienna, Dept Chem Ecol & Ecosyst Res, Vienna, Austria

Доп.точки доступа:
Shibistova, O...; Yohannes, Y...; Boy, J...; Richter, A...; Wild, B...; Watzka, M...; Guggenberger, G...

[Text] / G. G. Polyakova, V. V. Stasova, N. V. Pashenova // Russ. J. Plant Physiol. - 2011. - Vol. 58, Is. 5. - P819-827, DOI 10.1134/S1021443711050177. - Cited References: 23. - This work was supported by the Russian Foundation for Basic Research, project no. 09-04-09030. . - 9. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Ophiostomatoid fungi colonize the conducting tissues of conifer stems, the phloem and the xylem. These pathogenic fungi penetrate into the stem through injuries made by xylophagous insects vectoring these pathogens. In this study the response of the phloem of Scotch pine (Pinus sylvestris L.) to wounding (treatment 1) was compared with the response to wounding combined with application of high-molecular-weight compounds isolated from the mycelium of the ophiostomatoid fungus Ceratocystis laricicola Redfern & Minter (treatment 2). Both treatments induced the appearance of necrosis in the inner bark, the formation of periderm separating living and dead tissues, and formation of the callus alongside the wound perimeter. In addition, the bark accumulated lignin, bound proanthocyanidins, and resins, with a parallel decrease in the content of free proanthocyanidins, low-molecular-weight carbohydrates, and non-lignin components of the cell wall (P > 0.95). The size of necrotic spots, as well as changes in the content of most substances, were significantly higher in the treatment 2 than in the treatment 1 (P > 0.95). The accumulation of lignin in cell walls of phloem sieve cells was delayed in the treatment 2 as compared with that in the treatment 1. This suggested that the mycelial extract temporarily inhibited lignification at the early stage of the wound response. This disturbance of the cell wall protective transformation led to the hypothesis that the fungal suppressors retard the repair of inner bark injured by insects, thereby favoring the invasion of conifer tissues by ophiostomatoid fungi.

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Держатели документа:
[Polyakova, G. G.
Stasova, V. V.
Pashenova, N. V.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Stasova, V.V.; Pashenova, N.V.

[Text] / G. F. Antonova, V. V. Stasova // Russ. J. Dev. Biol. - 2008. - Vol. 39, Is. 4. - P207-218, DOI 10.1134/S1062360408040024. - Cited References: 82 . - 12. - ISSN 1062-3604РУБ Developmental Biology

Аннотация: The seasonal development of phloem in the stems of Siberian larch (Larix sibirica Ldb.) was studied over two seasons on 50-60-year-old trees growing in a natural stand in the Siberian forest-steppe zone. Trees at the age of 20-25 years were used to study metabolites in differentiating and mature phloem elements, cambial zone, and radially growing xylem cells in the periods of early and late wood formation. The development of the current-year phloem in the stems of 50-60-year-old trees started, depending on climatic conditions, in the second-third decades of May, 10-20 days before the xylem formation, and ended together with the shoot growth cessation in late July. Monitoring of the seasonal activity of cambium producing phloem sieve cells and the duration of their differentiation compared to the xylem derivatives in the cambium demonstrated that the top production of phloem and xylem cells could coincide or not coincide during the season, while their differentiation activity was always in antiphase. Sieve cells in the early phloem are separated from those in the late phloem by a layer of tannin-containing cells, which are formed in the period when late xylem formation starts. The starch content in the structural elements of phloem depends on the state of annual xylem layer development. The content of low molecular weight carbohydrates, amino acids, organic acids, and phenols in phloem cells, cambial zone, and xylem derivatives of the cambium depends on the cell type and developmental stage as well as on the type of forming wood (early or late) differing by the cell wall parameters and, hence, by the requirement for assimilates. Significant differences in the dynamics of substances per dry weight and cell were observed during cell development.

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Держатели документа:
[Antonova, G. F.
Stasova, V. V.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Antonova, G.F.; Stasova, V.V.

[Text] / G. G. Polyakova [et al.] // Russ. J. Plant Physiol. - 2008. - Vol. 55, Is. 4. - P496-502, DOI 10.1134/S1021443708040109. - Cited References: 25 . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: The elicitor activity of compounds extracted from the mycelia of six species of phytopathogenic fungi was assessed from the sizes of necrotic lesions on the external surface of the living trunk phloem of five coniferous species inhabiting Siberia: Siberian larch (Larix sibirica L.), Scotch pine (Pinus sylvestris L.), Siberian spruce (Picea obovata Ledeb.), Siberian fir (Abies sibirica L.), and cedar pine (Pinus sibirica (Rupr.) Mayr.). The compounds for inoculation were extracted from the mycelium of ascomycetes imperfect, and basidium fungi; the living mycelia of these fungi were also used. The fungal extract or mycelium was placed into the hollows 7 mm in diameter in the trunk bark. Infection triggered the formation of hypersensitivity necrotic lesions in the inner bark exceeding in size those appeared after control wounding of four tree species (larch, pine, spruce, and cedar); fir was an exclusion. In experiments with tree trunks and conifer calluses, a dependence of immune response parameters (the sizes of necrotic lesions and the content of lignin and bound proanthocyanidins) on the quantity of the fungal preparation was elucidated. The largest necrotic lesions appeared after injection of 500 mu g of the fungal preparation into the hollow in the trunk, and its higher quantities did not increase the indices measured. The size of the necrotic lesion on the trunk bark is supposed to be used as a promising index characterizing the level of tree immunity and tolerance under various ecological conditions.

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Держатели документа:
[Polyakova, G. G.
Pashenova, N. V.
Polyakov, V. I.
Zrazhevskaya, G. K.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Pashenova, N.V.; Polyakov, V.I.; Zrazhevskaya, G.K.

[Text] / G. F. Antonova, V. V. Stasova, T. N. Varaksina // Russ. J. Plant Physiol. - 2009. - Vol. 56, Is. 2. - P190-199, DOI 10.1134/S1021443709020071. - Cited References: 30. - This work was supported by the Russian Foundation for Basic Research, project no. 06-04-49501. . - 10. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Changes in the levels of ascorbic acid (AA), its oxidized form, dehydroascorbic acid (DHA), and uronic acids as initial precursors for the AA synthesis were studied as related to the degree of xylem and phloem cell development in the course of early and late wood formation in the trunks of Scots pine (Pinus sylvestris L.). The cells of mature and conducting phloem, cambial zone, differently developed cells in the zones of cell enlargement and maturation were obtained by successive scraping tissue layers from trunk segments of 20-25-year-old trees; tissue identification was checked anatomically and histochemically. The contents of compounds tested were calculated per dry weight and per cell basis. We found great differences in the contents of AA and DHA and also in their ratio in dependence of the wood type developing in the pine trunks during growth period and on the stage of differentiation of xylem and phloem cells. Changes in the AA content during xylem cell differentiation were accompanied by changes in the content of uronic acids. The amounts of AA, DHA, and uronic acids were the highest at the stage of early lignification and reduced with tracheid maturation. The AA to DHA ratio changed differently in the course of early and late xylem lignification. It reduced from the start of lignification to the formation of early mature xylem and, in contrast, increased in mature late wood; this indicates a difference in the level of redox processes in these tissues.

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Держатели документа:
[Antonova, G. F.
Stasova, V. V.
Varaksina, T. N.] Russian Acad Sci, Sukachev Inst Forest, Siberian Div, Akad Gorodok, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Antonova, G.F.; Stasova, V.V.; Varaksina, T.N.; Russian Foundation for Basic Research [06-04-49501]

[Text] / V. P. Vetrova, V. V. Stasova, N. V. Pashenova ; ed.: F Lieutier, WJ Mattson, WJ Mattso // PHYSIOLOGY AND GENETICS OF TREE-PHYTOPHAGE INTERACTIONS - INTERNATIONAL SYMPOSIUM. Ser. COLLOQUES DE L INRA : INST NATL RECHERCHE AGRONOMIQUE, 1999. - International Symposium on Physiology and Genetics of Tree-Phytophage Interactions (AUG 31-SEP 05, 1997, GUJAN, FRANCE), Is. 90. - P287-297. - Cited References: 13 . - 11. - ISBN 0293-1915. - ISBN 2-7380-0883-6РУБ Plant Sciences + Forestry

Аннотация: Effect of defoliation on resistance response of Siberian fir, Abies sibirica Ledeb., to fungal inoculation was investigated within three even-aged fir stands damaged by Siberian moth, Dendrolimus superans sibiricus Tschetw., and located in the Krasnoyarsk Territory (Central Siberia). Histological changes in tissues, accompanying the resistance response were examined in 30 fir trees (90-130 years old) of different defoliation degree (50, 75, 100 %) six weeks after single inoculation with Leptographium sp, vectored by Monochamus urussovi Fisch. (Coleoptera: Cerambycidae). It was recorded that the rate and intensity of defence response of phloem and xylem tissues to inoculation as well as percent of trees infested by the insect-vector differed significantly in dependence of defoliation degree. Trees of 50 percent defoliation significantly differed from trees of more severe defoliation in all parameters of defence response and percentage of colonisation by the beetle. The trees defoliated to 75 and 100% did not differ significantly in defence response activity and the number of the trees attacked by M. urussovi Strong difference in resistance to inoculation and colonisation by the beetle was recorded between trees defoliated in the current year and in the previous year. All infested by M, urussovi trees were characterised by the epidemic level of frequency of blue-stain fungi associated with this insect. The tendency was recorded to a decreased frequency of the pathogenic Ophiostoma sp. and a more frequent occurrence of the saprophyte O. curvicollis in trees defoliated in previous year which lost ability to defend against wounding and fungal infection.

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Держатели документа:
Russian Acad Sci, Lab Forest Zool, Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Vetrova, V.P.; Stasova, V.V.; Pashenova, N.V.; Lieutier, F \ed.\; Mattson, WJ \ed.\; Mattso, WJ \ed.\

[Text] / M. V. Skomarkova [et al.] // Trees-Struct. Funct. - 2006. - Vol. 20, Is. 5. - P571-586, DOI 10.1007/s00468-006-0072-4. - Cited References: 55 . - 16. - ISSN 0931-1890РУБ Forestry

Аннотация: We investigated the variability of tree-ring width, wood density and C-13/C-12 in beech tree rings (Fagus sylvatica L.), and analyzed the influence of climatic variables and carbohydrate storage on these parameters. Wood cores were taken from dominant beech trees in three stands in Germany and Italy. We used densitometry to obtain density profiles of tree rings and laser-ablation-combustion-GC-IRMS to estimate carbon isotope composition (delta C-13) of wood. The sensitivity of ring width, wood density and delta C-13 to climatic variables differed; with tree-ring width responding to environmental conditions (temperature or precipitation) during the first half of a growing season and maximum density correlated with temperatures in the second part of a growing season (July-September). delta C-13 variations indicate re-allocation and storage processes and effects of drought during the main growing season. About 20% of inter-annual variation of tree-ring width was explained by the tree-ring width of the previous year. This was confirmed by delta C-13 of wood which showed a contribution of stored carbohydrates to growth in spring and a storage effect that competes with growth in autumn. Only mid-season delta C-13 of wood was related to concurrent assimilation and climate. The comparison of seasonal changes in tree-ring maximum wood density and isotope composition revealed that an increasing seasonal water deficit changes the relationship between density and C-13 composition from a negative relation in years with optimal moisture to a positive relationship in years with strong water deficit. The climate signal, however, is over-ridden by effects of stand density and crown structure (e.g., by forest management). There was an unexpected high variability in mid season delta C-13 values of wood between individual trees (-31 to -24 parts per thousand) which was attributed to competition between dominant trees as indicated by crown area, and microclimatological variations within the canopy. Maximum wood density showed less variation (930-990 g cm(-3) stop). The relationship between seasonal changes in tree-ring structure and C-13 composition can be used to study carbon storage and re-allocation, which is important for improving models of tree-ring growth and carbon isotope fractionation. About 20-30% of the tree-ring is affected by storage processes. The effects of storage on tree-ring width and the effects of forest structure put an additional uncertainty on using tree rings of broad leaved trees for climate reconstruction.

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Держатели документа:
Max Planck Inst Biogeochem, Jena, Germany
Russian Acad Sci, Inst Forest, SB, Krasnoyarsk 660036, Russia
Univ Calif Berkeley, ESPM Dept, Berkeley, CA 94720 USA

Доп.точки доступа:
Skomarkova, M.V.; Vaganov, E.A.; Mund, M...; Knohl, A...; Linke, P...; Boerner, A...; Schulze, E.D.

/ N. -S. Cho [et al.] // Journal of the Faculty of Agriculture, Kyushu University. - 2008. - Vol. 53, Is. 2. - P395-398 . - ISSN 0023-6152
Аннотация: A method for estimation of tree's condition on activity of defence response in phloem was checked in Pinus sylvestris L. stands located near Krasnoyarsk (Siberia, Russia). The length of necrosis caused by inoculation of phloem of living tree by Ceratocystis laricicola Redf. et Minter was used as the parameter being measured. The field experiments were carried out in two even-aged (about 60 years) pine stands that were approximately equal on structure, productivity, density and recreation loading, but differentiated on degree of industrial pollution. The two permanent sample plots (SP) were founded in the severely polluted stand. The other two SP were placed in the unpolluted pine forest. The amount of pine trees within each SP varied from 200 to 250. From 22 up to 37 pine-trees selected randomly within every SP were inoculated with C. laricicola mycelium (test 1) and its extract (test 2). One inoculation hole per one tree (diameter 7 mm) was made in stem at a height of 1.3 m. Application of the both agents caused necrosis in phloem around the place of inoculation. In the case of fungal inoculation (test 1), the average length of necrosis in the unpolluted forest exceeded significantly the same parameter in the polluted stand: 51.7-79.4mm and 39.4-41.3mm, correspondingly. The action of the fungal metabolites caused the opposite results: in the unpolluted stand the average size of necroses was smaller in comparison with this parameter in the polluted stands 44.5-15.3 mm and 57.9-61.8 mm. The reasons of this difference are discussed. The both agents (C. laricicola mycelium and its extract) were suitable to reveal the difference of tree's condition in polluted and unpolluted pine stands. The application of fungal extract is more preferable in comparison with fungal mycelium because of smaller variability of necrosis size. Besides, the application of extract allows controlling inoculum dose and excludes the dangerous of spreading infection in forests.

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Держатели документа:
Department of Forest and Forest Products Sciences, Laboratory of Forest Resources Management, Division of Forest Ecosphere Management, Sasaguri, Fukuoka 811-2415, Japan
Wood and Paper Science, Chungbuk National University, Cheongju 361-763, South Korea
Department of Physical and Chemical Biology and Biotechnology of Woody Plants, V. N. Sukachev Institute of Forest SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Cho, N.-S.; Pashenova, N.V.; Choi, T.-H.; Ohga, S.

/ G. F. Antonova, V. V. Stasova // Russian Journal of Developmental Biology. - 2006. - Vol. 37, Is. 5. - P306-320, DOI 10.1134/S1062360406050043 . - ISSN 1062-3604
Аннотация: The formation of phloem was studied for two years in stems of 50 to 60 year old trees of Scots pine (Pinus sylvestris L.) growing in nature. The development of phloem of the current year begins 10 to 20 days before the xylem formation and is completed with the termination of shoot growth in the end of June. Observations over the seasonal activity of cambium producing sieve cells of phloem and duration of their differentiation as compared to the xylem derivatives of cambium have shown that the maxima of formation of phloem and xylem cells could coincide or not coincide by season, while the activities of their differentiation were always in antiphase. The sieve cells of early phloem were separated from those of late phloem by a layer of tannin-containing cells, which are formed simultaneously with the formation of late xylem cells by the cambium. Seasonal dynamics of accumulation of starch grain in structural elements of the phloem is related to the xylem development. The content of metabolites in differentiating and mature phloem elements, in the cambium zone, and in the xylem cells growing in the radial direction depended on cell specificity, stage of their development, and type of forming wood, early or late, which differ in the cell wall parameters and, hence, requirement of assimilates. Significant differences were described between the content of low molecular weigh carbohydrates, amino acids, organic acids, and phenol compounds using two methods of calculation: per dry weight and per cell. В© Nauka/Interperiodica 2006.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Antonova, G.F.; Stasova, V.V.

[Text] / G. G. POLYAKOVA [et al.] // Russ. J. Plant Physiol. - 1995. - Vol. 42, Is. 4. - P552-557. - Cited References: 21 . - 6. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: The rate of accumulation and location of proanthocyanidins and lignin was studied in fir (Abies sibirica Ledeb.) trunk phloem inoculated with two species of pathogenic micromycetes Verticillium sp. and Thysanophora sp., the usual associates of the sawyer Monochamus urussovi Fisch. Greater accumulation of the phenolic substances and more rapid localization of pathogen as a result of a hypersensitivity reaction were detected in firs infected with Verticillium sp. It was concluded that accumulation of proanthocyanidins and lignin is a part of nonspecific immune response of fir to the pathogen action and that the pathogen specific of fir can inhibit induced synthesis of defensive substances by the host plant.


Доп.точки доступа:
POLYAKOVA, G.G.; VETROVA, V.P.; PASHENOVA, N.V.; OSIPOV, V.I.

/ N. E. Sudachkova [et al.] // Contemp. Probl. Ecol. - 2015. - Vol. 8, Is. 1. - P21-27, DOI 10.1134/S199542551501014X . - ISSN 1995-4255
Аннотация: The effect of spring defoliation on different aspects of growth and metabolism of 10-year Scots pine trees (Pinus silvestris L.) in natural stands of the Krasnoyarsk forest-steppe region have been studied. These aspects include the structure of annual xylem and phloem increments, the content of starch and photosynthetic pigments in phloem, and the activity of antioxidative enzymes in xylem and phloem. It is shown that defoliation reduces shoot elongation, as well as the length and mass of needles and width of annual rings in xylem and phloem. It also stimulates late wood formation, an increase in total contents of photosynthetic pigments in the needles of newly formed shoots, and a reduction of starch contents in stem phloem. A significant increase of peroxidase activity in the xylem of defoliated trees can be a sign of oxidative stress. Defoliation has an evident negative effect on the formation of the annual rings of xylem, but does not have that strong an impact on phloem genesis. In general, during defoliation, the reserve function of the trees prevails over the growth function.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/28Krasnoyarsk, Russian Federation
ИЛ СО РАН

Доп.точки доступа:
Sudachkova, N.E.; Milyutina, I.L.; Romanova, L.I.; Astrakhantseva, N.V.

[Text] / K. T. Rinne [et al.] // Tree Physiol. - 2015. - Vol. 35, Is. 11. - P1192-1205, DOI 10.1093/treephys/tpv096. - Cited References:61. - This work was supported by Swiss National Science Foundation (REQIP, 206021_128761 to R.T.W.S., 200021_121838, 200020_134864, 206021_128761), Russian Science Foundation (14-14-00295 to A.V.K. and M.V.B.) and Paul Scherrer Institute (Instrumental upgrade funds to R.T.W.S.); N.J.L. acknowledges the UK Natural Environment Research Council (NE/501504), Climate Change Consortium for Wales (C3W) and European Commission 6th Framework Project 'Millennium' (017008). . - ISSN 0829-318X. - ISSN 1758-4469РУБ Forestry

Аннотация: Significant gaps still exist in our knowledge about post-photosynthetic leaf level and downstream metabolic processes and isotopic fractionations. This includes their impact on the isotopic climate signal stored in the carbon isotope composition (delta C-13) of leaf assimilates and tree rings. For the first time, we compared the seasonal delta C-13 variability of leaf sucrose with intra-annual, high-resolution delta C-13 signature of tree rings from larch (Larix gmelinii Rupr.). The trees were growing at two sites in the continuous permafrost zone of Siberia with different growth conditions. Our results indicate very similar low-frequency intra-seasonal trends of the sucrose and tree ring delta C-13 records with little or no indication for the use of 'old' photosynthates formed during the previous year(s). The comparison of leaf sucrose delta C-13 values with that in other leaf sugars and in tree rings elucidates the cause for the reported C-13-enrichment of sink organs compared with leaves. We observed that while the average delta C-13 of all needle sugars was 1.2% more negative than delta C-13 value of wood, the delta C-13 value of the transport sugar sucrose was on an average 1.0% more positive than that of wood. Our study shows a high potential of the combined use of compound-specific isotope analysis of sugars (leaf and phloem) with intra-annual tree ring delta C-13 measurements for deepening our understanding about the mechanisms controlling the isotope variability in tree rings under different environmental conditions.

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Держатели документа:
Paul Scherrer Inst, Lab Atmospher Chem, CH-5232 Villigen, Switzerland.
SB RAS, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Swansea Univ, Dept Geog, Swansea SA2 8PP, W Glam, Wales.
ETH, Inst Agr Sci, Zurich, Switzerland.

Доп.точки доступа:
Rinne, K. T.; Saurer, M.; Kirdyanov, A. V.; Loader, N. J.; Bryukhanova, M. V.; Werner, R. A.; Siegwolf, R. T. W.; Swiss National Science Foundation (REQIP) [206021_128761, 200021_121838, 200020_134864]; Russian Science Foundation [14-14-00295]; Paul Scherrer Institute; European Commission [017008]

[Text] : научное издание / G.F. Antonova, V.V. Stasova, N.V. Astrakhantseva // Сибирский лесной журнал. - 2017. - № : 1. - P25-36 : табл., DOI 10.15372/SJFS20170103. - Библиогр. в конце ст. . - ISSN 2311-1410

УДК

581.176

581.19

577.164.2

Аннотация: The contents of ascorbate (AsA) and dehydroascorbate (DHA) and their ratio, showing cellular redox state of AsA, were studied in the cells of the separate tissues at different levels of <i>Pinus sylvestris</i> L. stem during early- and latewood formation. Morphological status of the cells in the tissues and the content of soluble carbohydrates were also estimated. The cellular redox potential of AsA has been found to depend on the type of tissue, cell development degree, the level of stem and the type of forming wood. The content of AsA and AsA/DHA ratio in the cells of non-conducting phloem along the stem were higher than in mature xylem and less during earlywood than latewood formation. The cells of conducting phloem and forming xylem, as the principal tissues taking part in annual ring wood formation, differed in the content of acids in the course of early and late xylem formation. Along the stem, the content of AsA decreased in conducting phloem cells and increased in the cells of forming xylem during both early- and latewood formation. The AsA/DHA of conducting phloem during earlywood formation was greatest below the stem and diminished to the top of the tree, while in the course of latewood development it was similar at all levels. In forming xylem AsA/DHA increased to the top of tree during the early xylem formation and decreased in late xylem that indicates the differences in oxidation-reduction reactions into the cells of two type of forming wood. The data are discussed according to morphological development of cells and the content of carbohydrates.
The contents of ascorbate (AsA) and dehydroascorbate (DHA) and their ratio, showing cellular redox state of AsA, were studied in the cells of the separate tissues at different levels of <i>Pinus sylvestris</i> L. stem during early- and latewood formation. Morphological status of the cells in the tissues and the content of soluble carbohydrates were also estimated. The cellular redox potential of AsA has been found to depend on the type of tissue, cell development degree, the level of stem and the type of forming wood. The content of AsA and AsA/DHA ratio in the cells of non-conducting phloem along the stem were higher than in mature xylem and less during earlywood than latewood formation. The cells of conducting phloem and forming xylem, as the principal tissues taking part in annual ring wood formation, differed in the content of acids in the course of early and late xylem formation. Along the stem, the content of AsA decreased in conducting phloem cells and increased in the cells of forming xylem during both early- and latewood formation. The AsA/DHA of conducting phloem during earlywood formation was greatest below the stem and diminished to the top of the tree, while in the course of latewood development it was similar at all levels. In forming xylem AsA/DHA increased to the top of tree during the early xylem formation and decreased in late xylem that indicates the differences in oxidation-reduction reactions into the cells of two type of forming wood. The data are discussed according to morphological development of cells and the content of carbohydrates.

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Solitary Unit V. N. Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch

Доп.точки доступа:
Antonova, Galina Feodosiyevna; Stasova, Victoria Victorovna; Стасова, Виктория Викторовна; Astrakhantseva, Natalia Vladimirovna; Астраханцева, Наталья Владимировна; Антонова, Галина Феодосиевна

/ N. V. Pashenova [et al.] // Russ. J. Biol. Invasions. - 2018. - Vol. 9, Is. 1. - P63-74, DOI 10.1134/S2075111718010137 . - ISSN 2075-1117
Аннотация: The study is aimed at investigation of ophiostomatoid fungi, associated with the four-eyed fir bark beetle (Polygraphus proximus Blandford, Coleoptera, Curculionidae)—an invasive pest of Siberian fir Abies sibirica. In the Russian Far East, eight fungal species are associated with this bark beetle. All of these fungi (except Leptographium taigense and Grosmannia europhioides) were brought by P. proximus to its secondary range (Southern Siberia and Moscow oblast). In the secondary range, a complex of ophiostomatoid fungi connected with P. proximus consists mainly of the Far Eastern species adapted to new habitats. Phytopathogenic activity of the fungi associated with bark beetle was studied. G. aoshimae and Ophiostoma nikkoense demonstrated high phytopathogenic activity after inoculation into phloem of Siberian fir. The culture of G. aoshimae and O. subalpinum produced extensive necroses in stems of Siberian larch Larix sibirica. In general, G. aoshimae is the main fungal associate of P. proximus, considering the frequency and stability of its occurrence in samples, as well as its aggressiveness toward coniferous species of Southern Siberia. It demonstrated high aggressiveness toward Siberian fir and Siberian larch; Scots pine Pinus sylvestris is also susceptible to this fungus. Siberian spruce Picea obovata and Siberian pine Pinus sibirica are resistant. There is a high possibility of P. proximus mycobiota enrichment with a complex of fungi connected with fir sawyer beetle Monochamus urussovi Fisch., an aboriginal pest of fir in Siberia. © 2018, Pleiades Publishing, Ltd.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk, Russian Federation
Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, pr. Lavrent’eva 10, Novosibirsk, Russian Federation

Доп.точки доступа:
Pashenova, N. V.; Kononov, A. V.; Ustyantsev, K. V.; Blinov, A. G.; Pertsovaya, A. A.; Baranchikov, Y. N.

[Текст] : статья / Г. Г. Полякова, Н. В. Пашенова, В. А. Сенашова // Известия РАН. Серия Биологическая. - 2020. - № 1. - С. 44-50, DOI 10.31857/S0002332920010105 . - ISSN 0002-3329
   Перевод заглавия: Influence of Chronical Partial Defoliation of Larch by Casebeareron Stem Physiological Parameters

УДК	581.1:632.938

Аннотация: В очаге лиственничной чехликовой моли Coleophora sibiricella Falkovitsh в чистом лиственничнике исследован защитный ответ флоэмы ствола лиственницы сибирской Larix sibirica Ledeb. на поранение или инокуляцию ствола грибным индуктором. В качестве грибного инокулята использовали мицелий офиостомового гриба Ceratocystis laricicola Redfern & Minter, его экстракт и белковую фракцию, полученную из экстракта. Содержание лигнина, крахмала, конденсированных дубильных веществ (проантоцианидинов) во флоэме определено в начале опыта (0 сут), через 7 и 14 сут. С помощью анализа ANOVA показано, что параметры ответа флоэмы не зависели от формы грибного инокулята (р ≥ 0.918), но зависели от других факторов (р ≤ 0.005) – повреждения кроны молью, времени от начала опыта, наличия инокулята в ране
In the long-term explosion of mass reproduction of larch casebearer Coleophora sibiricella Falkovitsh in pure larch stand the stem phloem responses of larch trees (Larix sibirica Ledeb.) to wounding damage or inoculation of the trunk with the fungal inductor. Mycelium of the ophiostomoid fungus Ceratocystis laricicola Redfern & Minter, its extractive and protein fraction obtained from the extractive were used as a fungal inductor. The content of lignin, starch, condensed tannins (proanthocyanidins) in the phloem was determined at the beginning of the experiment (0 days), after 7 and 14 days. Using ANOVA analysis, it was shown that the response parameters of the phloem did not depend on the shape of the fungal inoculum (p ≥ 0.918), but depended on other factors (p ≤ 0.005) - damage to the crown by the moth, time from the start of the experiment, and the presence of the inoculum in the wound

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Держатели документа:
Институт леса им. В.Н. Сукачева СО РАН

Доп.точки доступа:
Полякова, Галина Геннадьевна; Polyakova Galina Gennad'yevna; Пашенова, Наталья Вениаминовна; Pashenova, Natal'ya Veniaminovna; Сенашова, Вера Александровна; Syenashova Vera Alexandrovna

/ G. G. Polyakova, N. V. Pashenova, V. A. Senashova // Biol. Bull. - 2020. - Vol. 47, Is. 1. - P49-55, DOI 10.1134/S1062359020010100. - Cited References:28. - This work was supported by the Russian Foundation for Basic Research, project no. 15-04-06575. . - ISSN 1062-3590. - ISSN 1608-3059РУБ Biology

Аннотация: In the long-term explosion of mass reproduction of the larch casebearer (Coleophora sibiricella Falkovitsh) in a pure larch stand, the stem phloem responses of larch trees (Larix sibirica Ledeb.) to wounding damage or inoculation of the trunk with the fungal inductor were studied. The mycelium of the ophiostomatoid fungus Ceratocystis laricicola Redfern & Minter and its extractive and the protein fraction obtained from the extractive were used as a fungal inductor. The content of lignin, starch, and condensed tannins (proanthocyanidins) in the phloem was determined at the beginning of the experiment (zero days), and after seven and 14 days. Using ANOVA analysis, it was shown that the response parameters of the phloem did not depend on the kind of the fungal inoculum (P = 0.918), but depended on other factors (P

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Держатели документа:
Sukachev Inst Forest, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Polyakova, G. G.; Pashenova, N. V.; Senashova, V. A.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [15-04-06575]

/ G. Polyakova, N. Pashenova, V. Senashova [et al.] // Environments. - 2020. - Vol. 7, Is. 4. - Ст. 28, DOI 10.3390/environments7040028. - Cited References:35. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, projects 0356-2018-0742 and 0356-2017-0017, and by RFBR grant 15-04-06575, RFBR-Krasnoyarsk Regional Foundation N 18-44-240004, grant 18-44-242002. . - ISSN 2076-3298РУБ Environmental Sciences

Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middle-aged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich's model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye's adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown.

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Держатели документа:
FRC KSC SB RAS, Sukachev Inst Forest SB RAS, Krasnoyarsk 660036, Russia.
Nonstate Educ Inst Higher Profess Educ Siberian I, Krasnoyarsk 660069, Russia.
FRC KSC SB RAS, Photobiol Lab, Inst Biophys SB RAS, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Biophys Dept, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Polyakova, Galina; Pashenova, Natalia; Senashova, Vera; Podolyak, Natalia; Kudryasheva, Nadezhda; Russian Academy of SciencesRussian Academy of Sciences [0356-2018-0742, 0356-2017-0017]; RFBRRussian Foundation for Basic Research (RFBR) [15-04-06575]; RFBR-Krasnoyarsk Regional Foundation [18-44-240004, 18-44-242002]

/ G. Polyakova, N. Pashenova, V. Senashova [et al.] // Environ. - MDPI. - 2020. - Vol. 7, Is. 4. - Ст. 28, DOI 10.3390/environments7040028 . - ISSN 2076-3298
Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middleaged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich‘s model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye‘s adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown. © 2020 by the authors.

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Держатели документа:
Sukachev Institute of Forest SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Non-State Educational Institution of Higher Professional Education Siberian Institute of Business, Management and Psychology, Krasnoyarsk, 660069, Russian Federation
Photobiology Lab, Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, 660036, Russian Federation
Biophysics Department, Siberian Federal University, Krasnoyarsk, 660049, Russian Federation

Доп.точки доступа:
Polyakova, G.; Pashenova, N.; Senashova, V.; Podolyak, N.; Kudryasheva, N.

/ Н. В. Астраханцева, Л. Г. Серая, Н. В. Пашенова, А. А. Коженкова, Ю. Н. Баранчиков // Сибирский лесной журнал. - 2023. - № 5. - С. 43-59DOI 10.15372/SJFS20230507
   Перевод заглавия: ANATOMICAL FEATURES OF THE BARK AS A FACTOR OF RESISTANCE OF FIR SPECIES TO INFESTATION BY THE FOUR-EYED FIR BARK BEETLE

ГРНТИ

68.47.03

Аннотация: Для выяснения причин различной видовой повреждаемости пихт ( Abies Mill.) уссурийским полиграфом ( Polygraphus proximus Blandford) изучали строение коры представителей секций Balsamea , Momi , Abies , Grandis в коллекциях Главного ботанического сада им. Н. В. Цицина РАН. Исследовали особенности структуры покровных тканей (перидермы и ритидома) и внутренней (живой) коры, состоявшей из первичной коры (кортекса) и вторичной флоэмы. Виды пихт, покровные ткани ствола которых образованы перидермами, включающими слои губчатой и каменистой (лигнифицированной) феллемы, - пихта цельнолистная ( Abies holophylla Maxim.), п. равночешуйчатая ( A. homolepis Siebold & Zucc.) , п. Нордмана ( A. nordmanniana (Steven) Spach), а также виды, рано формирующие корку, - п. Нордмана, полиграфом практически не заселялись. Виды пихт, покровные ткани которых имели малую толщину и были представлены однотипными суберинизированными клетками феллемы, оказались наиболее привлекательны для втачивания полиграфа (виды из секции Balsamea ). Отмечена связь повреждаемости уссурийским полиграфом с особенностями строения и соотношения компонентов первичной коры и вторичной флоэмы, где основным фактором служила степень склерификации. Наименее привлекательными для уссурийского полиграфа были виды, имеющие высокую степень склерификации внешнего и среднего слоя внутренней коры. Степень конституционной устойчивости к уссурийскому полиграфу повышалась с увеличением степени срастания и размеров склереидных групп, равномерности и плотности их распределения. Наиболее уязвимыми были пихты с низкой степенью склерификации внешнего и среднего слоя и/или неравномерным и неплотным распределением групп склереид, и пихты, имеющие повышенное и высокое содержание слизевых клеток (п. сибирская ( A. sibirica Ledeb.)). Отмечена обратная связь между степенью склерификации и содержанием слизевых клеток. Четкой зависимости между повреждаемостью полиграфом и содержанием кристаллов оксалата кальция, а также особенностями смолоносной системы не установлено.
To elucidate the reasons for the differences Fir species ( Abies . Mill.) damage by four-eyed fir bark beetle ( Polygraphus proximus Blandford), we studied the structure of fir bark in sections Balsamea , Momi , Abies , Grandis from the collections of the Tsitsin Main botanical garden of the Russian Academy of Sciences. In the bark, the features of the structure of the outer bark tissues (periderm and rhytidome) and the inner bark, which consisted of the cortex and secondary phloem, were studied. Species of fir, the outer bark of which are formed by periderms including layers of spongy and stony (lignified) phellem (phellem of Manchurian fir ( Abies holophylla Maxim.), Nikko fir ( A. homolepis Siebold & Zucc.), Nordmann fir ( A. nordmanniana (Steven) Spach)), as well as species that early formed a rhytidome (Nordmann fir), are practically not colonized by the P. proximus . The cork of fir species, the phellem of which is represented by the same type of suberinized cells, and having a small thickness (species from section Balsamea ), is more attractive for invasion by the P. proximus . The relationship between damage by the P. proximus and the structural features and the ratio of the components of the outer and middle layers of the inner bark, where the degree of sclerification is the main factor, is noted. The least attractive for P. proximus are the fir species that have a high degree of sclerification of the outer and middle layers of the inner bark. The degree of constitutional resistance to P. proximus increases with an increase in the degree of accretion and size of sclereid groups, the uniformity and density of their distribution. The most vulnerable are firs with a low degree of sclerification of the outer and middle layers and/or an uneven and loose distribution of sclereid groups, and firs with an increased and high content of mucilage cells (Siberian fir ( A. sibirica Ledeb.)). An inverse relationship was noted between the degree of sclerification and the content of mucilage cells. A clear relationship between P. proximus and the content of calcium oxalate crystals, as well as the characteristics of the resinous system, has not been established.

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ИЛ СО РАН : 660036, Красноярск, Академгородок 50/28

Доп.точки доступа:
Серая, Лидия Георгиевна; Seraya, Lidiya Georgievna; Пашенова, Наталья Вениаминовна; Pashenova, Natal'ya Veniaminovna; Коженкова, А.А.; Баранчиков, Юрий Николаевич; Baranchikov, Yury Nikolayevich; Astrakhantseva Natal'ya Vladimirovna