Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / O. A. Shapchenkova, Y. N. Krasnoshchekov, S. R. Loskutov // Eurasian Soil Sci. - 2011. - Vol. 44, Is. 6. - P677-685, DOI 10.1134/S1064229311060123. - Cited References: 12. - This stugy was supported by the Russian Foundation for Basic Research project no. 08-04-00027. . - 9. - ISSN 1064-2293РУБ Soil Science

Аннотация: Experimental data on the effect of surface fires on the organic matter transformation in the gray-humus soils of pine forests were obtained in the southwestern part of the Baikal region. The application of methods of thermal analysis (such as differential scanning calorimetry and thermogravimetry) made it possible to obtain qualitative and quantitative information about the decomposition (oxidation) of the components of the soil organic matter upon their heating. It was found that the organic matter content in the soils subjected to fires of high intensity decreased by 1.9 times in comparison with the control. In the litter horizons of the undisturbed soils, thermolabile components (mostly, oligo- and polysaccharides) comprised 61% of the organic matter, and the portion of thermostable components (aromatic compounds) was 39%. A significant decrease in the content of thermolabile components and an increase in the content of thermostable components (up to 62%) were observed in the organic matter of the postpyrogenic forest litter as a result of the charcoal formation during the fire.

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Держатели документа:
[Shapchenkova, O. A.
Krasnoshchekov, Yu. N.
Loskutov, S. R.] Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Shapchenkova, O.A.; Krasnoshchekov, Y.N.; Loskutov, S.R.

[Text] / O. A. Shapchenkova, A. A. Aniskina, S. R. Loskutov // Eurasian Soil Sci. - 2011. - Vol. 44, Is. 4. - P399-406, DOI 10.1134/S1064229311040090. - Cited References: 29. - This work was supported by Integration Project no. 23 (1.3) of the Siberian Branch of the Russian Academy of Sciences (Succession Changes in the Biodiversity in Technogenically Disturbed Ecosystems of Siberia). . - 8. - ISSN 1064-2293РУБ Soil Science

Аннотация: The thermal degradation of organic matter was studied in cryogenic soils with methods of thermal analysis: differential scanning calorimetry and thermogravimetry (DSC and TG, respectively). The DSC curves of most of the samples within the temperature range from 221-247A degrees C to 600A degrees C were characterized by the presence of one wide exothermic peak (at 311-373A degrees C) with a shoulder (or without it) on the descending branch at a temperature of about 400A degrees C. This was connected mostly with the destruction of thermolabile compounds (oligo- and polysaccharides) and with the oxidation of low-aromatic complexes of plant residues and humus substances. Two exothermic peaks at 337-373A degrees C and 448-492A degrees C were found for some samples from the organic horizons. The high-temperature peaks were caused by the thermal destruction of lignin. The fraction of the thermolabile organic matter of the soil (237-261aEuro broken vertical bar 331-377A degrees C) reached 59-73% in the organic and 52-59% in the organomineral and mineral horizons.

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Держатели документа:
[Shapchenkova, O. A.
Aniskina, A. A.
Loskutov, S. R.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Shapchenkova, O.A.; Aniskina, A.A.; Loskutov, S.R.

[Text] / T. T. Efremova, S. P. Efremov, N. V. Melent'eva // Eurasian Soil Sci. - 1997. - Vol. 30, Is. 12. - P1318-1325. - Cited References: 44 . - 8. - ISSN 1064-2293РУБ Soil Science

Аннотация: The reserves of carbon in peat deposits and peaty and peat bog soils of Russia comprise 44.2 Gt C of humic substances (37.3% of the total reserve of C), 44.7 Gt C (37.8%) of insoluble residue, and 21.1 Gt C (17.8%) of polysaccharides, The carbon pool of humic acids constitutes 25.0 Gt and that of fulvic acids reaches 19.1 Gt. Humic acids predominate in low-moor peat, whereas fulvic acids predominate in high-moor peat. Transitional pear deposits contain approximately equal amounts of fulvic and humic acids. About 89-91% of the carbon of humic substances in different types of peat is represented by heterogeneous complexes of the first and third fractions.

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Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forestry, Akademgorodok 660036, Krasnoyarsk, Russia

Доп.точки доступа:
Efremova, T.T.; Efremov, S.P.; Melent'eva, N.V.

[Text] / G. F. Antonova, I. A. Chapligina ; ed.: R Lagana, S Kurjatko, , S Kurjat // WOOD STRUCTURE AND PROPERTIES' 06 : ARBORA PUBLISHERS, 2006. - 5th International Symposium Wood Structure and Properties (SEP 03-06, 2006, Sliac Siehnica, SLOVAKIA). - P25-31. - Cited References: 22 . - 7. - ISBN 978-80-968869-4-4РУБ Materials Science, Paper & Wood

Аннотация: The deposition of cellulose, matrix polysaccharides and lignin during secondary cell wall development of early- and latewood in larch (Larix sibirica Ldb.) were studied using biochemical and histochemical methods. Active tissues at successive stages of secondary cell wall development were sampled from larch trunk and characterized by morphological parameters and the content of cellulose, lignin and matrix hemicelluloses, being the structural base for lignification. Matrix polysaccharides were divided by their solubility in 4 % and 24 % KOH and neutral medium. All data were accounted per dry weight and per cell. The amount of biomass (as cell wall cross-section area) deposited before lignification was more in earlywood tracheids than in latewood ones. In contrast. the amount of cellulose at this stage was more in latewood tracheids. The intensity of cellulose synthesis was the highest at the initial stage of lignin deposition in both earlywood and latewood and then declined towards to mature xylem. The intensity of lignin deposition was the highest at the last stage of secondary cell wall development during earlywood formation and from the outset of latewood lignification, dropping towards mature xylem. The contents of A and B hemicellulose fractions linked or not with cellulose, before and after beginning of lignification were different during earlywood and latewood formation. Before lignin deposition, the content of B-4 fraction in both earlywood and latewood was practically equal as well as of B-24, while the amount of A-4 fraction before lignification of early xylem was two times more than that of latewood. This shows different matrix base for the beginning of lignification. All together can be the reason for different physicochemical properties of two types of wood in larch.

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Держатели документа:
Russian Acad Siences, SB, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Antonova, G.F.; Chapligina, I.A.; Lagana, R \ed.\; Kurjatko, S \ed.\; Kurjat, , S \ed.\

[Text] / T. T. Efremova [et al.] // Eurasian Soil Sci. - 2009. - Vol. 42, Is. 10. - P1120-1129, DOI 10.1134/S1064229309100068. - Cited References: 13. - This study was supported by the Russian Foundation for Basic Research, project no. 08-04-92501. . - 10. - ISSN 1064-2293РУБ Soil Science

Аннотация: In the litters (peaty, weakly decomposed, and strongly decomposed) of bog birch forests, the biochemical transformation of plant material includes several discrete stages. The best prediction of the decomposition stages is the set of indices characterizing the humus status of the soils: the C/N ratio, (I HA pound + I FA) pound/C(polysaccharides), and the contents of humic and fulvic acids of fraction 1 in the groups of plant debris of different sizes. The discrimination of the litters according to the level of the biochemical transformation of the organic residues was observed when using the (I HA pound + I FA) pound/C(polysaccharides) ratio, which is 6.3 in the peaty litter and 11.2 and 18.4 in the weakly decomposed and strongly decomposed litters, respectively. The biochemical classification of litters in the bog birch forests is in agreement with the morphological classification of litters.

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Держатели документа:
[Efremova, T. T.
Avrova, A. F.
Efremov, S. P.
Melent'eva, N. V.] Russian Acad Sci, Siberian Branch, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Efremova, T.T.; Avrova, A.F.; Efremov, S.P.; Melent'eva, N.V.; Russian Foundation for Basic Research [08-04-92501]

[Text] / G. F. ANTONOVA, V. V. STASOVA // SOVIET PLANT PHYSIOLOGY. - 1990. - Vol. 37, Is. 4. - P547-554. - Cited References: 23 . - 8. - ISSN 0038-5719РУБ Plant Sciences

Аннотация: We conducted a comparative study on the composition of water soluble carbohydrate-protein complexes of primary cell walls and cytoplasm in xylem cells of Siberian larch (Larix sibirica Ldb.). Preparations were obtained in the process of isolating protoplasts from xylem cells at the stage of elongation. Water-soluble high-molecular-weight compounds were fractionated on DEAE cellulose. It was found that the dominant fraction in composition of these compounds consists of a carbohydrate-protein complex. Comparison of the composition of neutral sugars, uronic acids, and amino acids of these complexes showed that water-soluble arabinogalactan proteins were absent in the cytoplasm, but present in the solution after plasmolysis. They were bound with pectins and arabinoxylans, detected in solutions obtained after isolation and purification of protoplasts, and capable of forming a gel at low water content and low temperature. It is hypothesized that arabinogalactan proteins are components of primary cell walls, that they are located for the most part on the surface, and that their solubility in an aqueous medium depends upon binding with pectins of the middle plate and with other cell wall components.

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Доп.точки доступа:
ANTONOVA, G.F.; STASOVA, V.V.

[Text] / G. F. ANTONOVA, E. S. MALYUTINA, V. V. SHEBEKO // SOVIET PLANT PHYSIOLOGY. - 1983. - Vol. 30, Is. 1. - P120-126. - Cited References: 21 . - 7. - ISSN 0038-5719РУБ Plant Sciences


Доп.точки доступа:
ANTONOVA, G.F.; MALYUTINA, E.S.; SHEBEKO, V.V.

/ Y. A. Litovka [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2018. - Is. 1. - С. 193-199, DOI 10.14258/jcprm.2018012729 . - ISSN 1029-5151
Аннотация: Presents the results of a research of wood-destroying properties of the basidiomycete Fomitopsis pinicola (Sw.) P. Karst. The strains were isolated in pure culture from fruiting bodies that grew on the Abies sibirica Ledeb, Pinus sylvestris Ledeb, Pinus sibirica Du Tour and Larix sibirica Ledeb in the forests of the Krasnoyarsk Territory (56°09'-56°22' N; 92°10'- 92°58' E). The cultures screened for growth parameters and the dynamics of enzymatic activity during cultivation on cellulose-containing media. The radial growth rate of Siberian strains on the agar medium and plant substrates is 1,1-3,3 mm / day; the growth coefficient is 10-42. All studied cultures synthesize extracellular cellulolytic enzymes that hydrolyze the sodium salt of carboxymethylcellulose. The enzymatic activity is in the range 0,05-0,39 U / ml with a maximum of 7-9 days. The most active strain of Fp5-15 F. pinicola used for bioconversion of coniferous plant substrates under solid-phase culture conditions. The maximum wood-destroying activity observed on pine and larch wood - the mass loss of substrates was 16 and 20%, respectively. On Pinus sylvestris sawdust, the total content of polysaccharides decreased by 1,2 times due to the fermentolysis of the hardly hydrolyzable fraction (a decrease of 1,4 times); the content of lignin substances did not change significantly. On the sawdust of Larix sibirica, the amount of polysaccharides decreased by 1,3 times in comparison with the initial substrate; lig-nin - in 1,2 times. © 2018 Altai State University. All rights reserved.

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Держатели документа:
Forest Institute, V.N. Sukachev Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 50-28, Krasnoyarsk, Russian Federation
Siberian State University of Science and Technology, Academician M.F. Reshetnev, Mira ave., 82, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Litovka, Y. A.; Pavlov, I. N.; Ryazanova, T. V.; Gazizulina, A. V.; Chuprova, N. A.

/ O. Moodley [et al.] // Bull. Environ. Contam. Toxicol. - 2018, DOI 10.1007/s00128-018-2496-0 . - Article in press. - ISSN 0007-4861
Аннотация: Alternaria oxytropis is an endophytic fungus of locoweeds that synthesizes swainsonine toxin. In this work, we evaluated the effect of A. oxytropis on soybean seedlings and quantified swainsonine in different culture conditions. Soybean (Glycine max) seeds were co-cultured with A. oxytropis (at different concentrations of mycelial suspensions) in agar media and soil culture, and swainsonine was assayed using LC–MS/MS. The results showed evidence that A. oxytropis infected soybean seedlings produced detectable swainsonine in agar culture while the toxin was undetectable or below the detection limit (0.006% of swainsonine dry weight) in soil media even at higher concentrations of the fungus. These results suggest that swainsonine detection is highly dependent on culture conditions and that soybeans co-cultured with A. oxytropis in soil could potentially be used to limit toxin production. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.

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Держатели документа:
Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin Province 130118, China
South African Department of Agriculture, Forestry and Fisheries (DAFF), Pretoria, 0001, South Africa
Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan
V.N. Sukachev Institute of Forest SB RAS, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Moodley, O.; Sun, Y.; Sossah, F. L.; Kakishima, M.; Pavlov, I. N.; Li, Y.; Wang, Q.

/ X. Huang [et al.] // Sci. Rep. - 2019. - Vol. 9, Is. 1. - Ст. 18684, DOI 10.1038/s41598-019-55174-y . - ISSN 2045-2322
Аннотация: The soil-water interfaces (SWI) in soil pores are hotspots for organic matter (OM) transformation. However, due to the heterogeneous and opaque nature of soil microenvironment, direct and continuous tracing of interfacial reactions, such as OM transformations and formation of organo-mineral associations, are rare. To investigate these processes, a new soil microarray technology (SoilChips) was developed and used. Homogeneous 800-?m-diameter SoilChips were constructed by depositing a dispersed Oxisol A horizon suspension on a patterned glass. Dissolved organic matter from the original soil was added on the SoilChips to mimic SWI processes. The effects of ammonium fertilization (90 mg N kg?1 soil) on chemical composition of SWIs were evaluated via X-ray photoelectron spectroscopy. Over 21 days, ammonium addition increased OM coatings at SWIs and modified the OM chemical structure with more alcoholic- and carboxylic-C compared to the unfertilized control. Molecular modeling of OM composition at SWIs showed that N fertilization mainly facilitated the microbial production of glucans. We demonstrated that N availability modifies the specific OM molecular processing and its immobilization on SWIs, thereby providing a direct insight into biogeochemical transformation of OM at micro-scale. © 2019, The Author(s).

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Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China
Key Laboratory of Agro-ecological Processes in the Subtropical Region, Institute of Subtropical Agriculture, The Chinese Academy of Sciences, Changsha, 410125, China
Changsha Research Station for Agricultural and Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, China
Institute of Soil Science, Leibniz Universitat Hannover, Hannover, 30419, Germany
VN Sukachev Institute of Forest, SB-RAS, Krasnoyarsk, 660036, Russian Federation
Department of Soil Science of Temperate Ecosystems, Department of Agricultural Soil Science, University of Goettingen, Gottingen, Germany
Institute of Environmental Sciences, Kazan Federal University, Kazan, 420049, Russian Federation
Agro-Technology Institute, RUDN University, Moscow, Russian Federation

Доп.точки доступа:
Huang, X.; Guggenberger, G.; Kuzyakov, Y.; Shibistova, O.; Ge, T.; Li, Y.; Liu, B.; Wu, J.

/ X. Z. Huang, G. Guggenberger, Y. Kuzyakov [et al.] // Sci Rep. - 2019. - Vol. 9. - Ст. 18684, DOI 10.1038/s41598-019-55174-y. - Cited References:47. - The authors gratefully acknowledge financial support from the National Natural Science Foundation of China (No. 41090283; No. 41430860; No. 41807051) as well as and National Science Foundation of China and Russian Foundation of Basic Research joint project (N 19-54-53026) granted to T.G. and O.S. The publication was supported by the Russian Government Program of Competitive Growth of Kazan Federal University and with the support of the "RUDN University program 5-100". Contribution of YK was supported by the Russian Science Foundation (project No. 19-77-30012). . - ISSN 2045-2322РУБ Multidisciplinary Sciences

Аннотация: The soil-water interfaces (SWI) in soil pores are hotspots for organic matter (OM) transformation. However, due to the heterogeneous and opaque nature of soil microenvironment, direct and continuous tracing of interfacial reactions, such as OM transformations and formation of organo-mineral associations, are rare. To investigate these processes, a new soil microarray technology (SoilChips) was developed and used. Homogeneous 800-mu m-diameter SoilChips were constructed by depositing a dispersed Oxisol A horizon suspension on a patterned glass. Dissolved organic matter from the original soil was added on the SoilChips to mimic SWI processes. The effects of ammonium fertilization (90 mg N kg(-1) soil) on chemical composition of SWIs were evaluated via X-ray photoelectron spectroscopy. Over 21 days, ammonium addition increased OM coatings at SWIs and modified the OM chemical structure with more alcoholic- and carboxylic-C compared to the unfertilized control. Molecular modeling of OM composition at SWIs showed that N fertilization mainly facilitated the microbial production of glucans. We demonstrated that N availability modifies the specific OM molecular processing and its immobilization on SWIs, thereby providing a direct insight into biogeochemical transformation of OM at micro-scale.

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Держатели документа:
Huazhong Univ Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Britton Chance Ctr Biomed Photon,Wuhan Natl Lab O, Dept Biomed Engn,Coll Life Sci & Technol,Syst Bio, Wuhan, Hubei, Peoples R China.
Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Hunan, Peoples R China.
Chinese Acad Sci, Inst Subtrop Agr, Changsha Res Stn Agr & Environm Monitoring, Hunan 410125, Peoples R China.
Leibniz Univ Hannover, Inst Soil Sci, D-30419 Hannover, Germany.
RAS, VN Sukachev Inst Forest, SB, Krasnoyarsk 660036, Russia.
Univ Goettingen, Dept Agr Soil Sci, Dept Soil Sci Temperate Ecosyst, Gottingen, Germany.
Kazan Fed Univ, Inst Environm Sci, Kazan 420049, Russia.
RUDN Univ, Agrotechnol Inst, Moscow, Russia.

Доп.точки доступа:
Huang, Xizhi; Guggenberger, Georg; Kuzyakov, Yakov; Shibistova, Olga; Ge, Tida; Li, Yiwei; Liu, Bifeng; Wu, Jinshui; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41090283, 41430860, 41807051]; National Science Foundation of ChinaNational Natural Science Foundation of China [N 19-54-53026]; Russian Foundation of Basic ResearchRussian Foundation for Basic Research (RFBR) [N 19-54-53026]; Russian Government Program of Competitive Growth of Kazan Federal University; Russian Science FoundationRussian Science Foundation (RSF) [19-77-30012]; RUDN University program 5-100

/ Zh. A. Koh, Yu. A. Litovka, P. V. Makolova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 395-403, DOI 10.14258/JCPRM.2020048530 . - ISSN 1029-5151
   Перевод заглавия: Биохимический состав вегетативных эксплантов и каллусов pinus sibirica du tour
Аннотация: The methods of sterilization of annual shoots Pinus sibirica Du Tour and the conditions for their introduction into in vitro culture were studied. Induction of callusogenesis of aseptically viable explants of P. sibirica proceeds more intensively on the modified Murasige-Skoog medium: hormonal supply of 0.4% kinetin and 0.25% 6-benzylaminopurine; reduced sucrose concentration of 1.5%. The frequency of callus formation was 83%. Close quantitative indicators of extractive substances were established (36 and 33% of absolutely dry weight for callus and explant, respectively); easily hydrolyzable polysaccharides (18 and 16%) and proteins (11 and 10%).Callus P. sibirica has a higher content of ascorbic acid, flavanoids, tocopherols and ash elements compared to explants and a low amount of hard-hydrolyzable polysaccharides, lipids, tannins, pigments, and essential oils.The electrophoretic spectrum of water-soluble callus proteins is represented by eleven fractions: 63% of the total water-soluble proteins are fractions with a molecular weight of 33 kD and above. Fractions with molecular weights of 50 and 62 kD (20 and 17%, respectively) are represented as much as possible.In the explants of P. sibirica, low molecular weight fractions of proteins with molecular masses of 5 kD and lower (59%) predominate. The amino acid composition of calli and explants of P. sibirica is identical and is represented by fifteen individual amino acids. Callus tissue has a higher content of glutamic acid and two hydrophobic amino acids (proline and isoleucine) compared to the vegetative part of the plant and low tyrosine content. © 2020 Altai State University. All rights reserved.

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Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation

Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Makolova, P. V.; Shabanova, K. A.; Pavlov, I. N.

/ Zh. A. Koh, Yu. A. Litovka, R. Kh. Enazarov [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 359-369, DOI 10.14258/JCPRM.2020048392 . - ISSN 1029-5151
   Перевод заглавия: Биотехнологические аспекты утилизации послеэкстракционной биомассы и клеточной культуры orthilia secunda (L.) house базидиальными грибами
Аннотация: The possibility of bioconversion after the extraction residue of the medicinal plant Orthilia secunda (initial plant biomass and callus after extraction of biologically active substances) by fast growing strains of basidiomycetes Tv2-16K Trametes versicolor and Pe-17T Pleurotus eryngii was studied. The main target products are: a mycelial-plant product with a reduced content of the indigestible fraction and enriched in protein and full-fledged fruit bodies. Fungi colonize plant waste with a radial growth rate of 2.0-2.3 mm / day and a growth rate of 65-77 units. Phenol oxidase activity is 0.7-1.2 units/g·s, depending on the type after the extraction residue. In substrates after biodegradation, the proportion of difficult hydrolysable polysaccharides, easily hydrolysable polysaccharides, and lignin is on average 1.6 times lower than in the original substrate. The content of protein and extractive substances is significantly higher, especially after the extraction residue of O. secunda callus under the influence of the T. versicolor strain Tv2-16K: 12.8 and 24.3%, respectively, of the mass of dry substrate. Fruit bodies of P. eryngii strain Pe-17T were obtained on various compositions of plant substrates. The maximum fruit formation was noted on four-component substrates containing two types after extraction residues of O. secunda, wheat bran, birch or aspen sawdust: the average weight of fruit bodies from one block was 230-236 g; biological efficiency - 46-47.2%. © 2020 Altai State University. All rights reserved.

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Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation

Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Enazarov, R. Kh.; Makolova, P. V.; Shimova, Yu. S.; Pochekutov, I. S.; Pavlov, I. N.

/ Yu. A. Litovka, I. N. Pavlov, P. V. Makolova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 371-383, DOI 10.14258/JCPRM.2020048396 . - ISSN 1029-5151
   Перевод заглавия: Биотехнологический потенциал сибирских штаммов базидиальных грибов - продуцентов ферментов лигноцеллюлазного действия
Аннотация: The results of a study of the wood-destroying properties of Siberian strains of xylotrophic basidiomycetes (Armillaria, Ganoderma, Fomitopsis, Heterobasidion and Porodaedalea) are presented. The growth parameters and enzymatic activity of the strains were determined during solid-phase and deep cultivation. Fomitopsis pinicola and Ganoderma lucidum are fast-growing fungi on cellulose-, tannin-containing nutrient media, coniferous and deciduous plant substrates (source and hydrodynamically activated). The growth coefficient on media with tannin and Na-carboxymethyl cellulose is more than 45; on plant substrates - more than 30. The strains exhibit a different growth reaction to the preliminary activation of birch sawdust (growth acceleration / growth slowdown / indifference). The maximum wood-destroying activity on wood of A. sibirica noted for the fungi A. borealis, G. tsugae, G. lucidum, F. pinicola, and P. niemelaei. The decrease in substrate mass was 8-11%; the amount of polysaccharides decreased on average 1.4 times mainly due to the fermentolysis of hard-hydrolyzable polysaccharides. High enzymatic activity of fungi observed during solid-phase and deep cultivation with an inducer. The maximum activity of phenol oxidase is characteristic of G. tsugae (1.21 units/g·s); carboxymethyl cellulase - for F.pinicola and G. lucidum strains (11.8 and 10.3 units/ml, respectively); xylanases - for H. abietinum (3.8 u/ml). The maximum accumulation of extracellular protein observed in F. pinicola (0.89 mg/ml). According to the totality of rapid test indicators, quantitative determination of enzymatic activity, growth parameters on lignocellulosic substrates and the degree of wood bioconversion, the most promising producer of lignolytic enzymes in vitro is the Gl4-16A Ganoderma lucidum strain; cellulolytic enzymes - strain Fp6-17 Fomitopsis pinicola. © 2020 Altai State University. All rights reserved.

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Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Institute of Physics L.V. Kirensky, SB, RAS, Akademgorodok, 50/43, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Litovka, Yu. A.; Pavlov, I. N.; Makolova, P. V.; Timofeev, A. A.; Litvinova, E. A.; Vasil'Eva, A. A.; Shabanov, A. V.

/ I. N. Pavlov, Yu. A. Litovka, E. A. Litvinova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 385-394, DOI 10.14258/JCPRM.2020048400 . - ISSN 1029-5151
   Перевод заглавия: Перспективы глубинного культивирования fomitopsis pinicola (SW.) P. karst. на гидродинамически активированных растительных отходах
Аннотация: The results of solid-phase and deep cultivation of the Siberian strain Fp6-17 Fomitopsis pinicola (Sw.) P. Karst. are presented. Growth parameters were determined on cellulose-containing agarized media and plant substrates with the addition of a solid phase after hydrodynamic activation of fir needles and sawdust of birch. Radial growth rate 3.0-4.3 mm / day; growth coefficient 37-64. Changes in polysaccharides in activated birch sawdust during fermentation noted, despite the low growth coefficient of the fungus. After hydrodynamic activation, the content of easily hydrolysable polysaccharides increased by 9 %, the proportion of hardly hydrolysable polysaccharides decreased by 8%. After further solid-phase cultivation, the content of easily and hardly hydrolysable polysaccharides decreased by 10 and 23%, respectively. The conditions for the deep cultivation of the strain Fp6-17 on a modified Norkrans medium with microcrystalline cellulose selected. At 26 °C and a pH of 4.6 on the seventh day, the maximum activity of carboxymethyl cellulase was 13.6 units / ml; biomass yield - 16.2 g/l. The optimal composition of the solid phase medium was selected after hydrodynamic processing of fir needles and sawdust of birch with enrichment of (NH4)2SO4. The maximum activity of the enzyme was 14.2 u/ml. With high enzymatic activity in the culture fluid, the formation of small globules with a diameter of up to 5 mm and a smaller amount of biomass is noted. At lower enzyme indices, in most cases a greater increase in biomass recorded due to the formation of large ellipsoid pellets with a fringed edge. © 2020 Altai State University. All rights reserved.

Scopus

Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk Scientific Center, SB, RAS, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Pavlov, I. N.; Litovka, Yu. A.; Litvinova, E. A.; Petrenko, S. M.; Enazarov, R. Kh.

/ T. V. Ryazanova, V. S. Fedorov, E. V. Kharyanova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 351-358, DOI 10.14258/JCPRM.2020048520 . - ISSN 1029-5151
   Перевод заглавия: ПЕРОКСИДНАЯ ДЕЛИГНИФИКАЦИЯ ВЕГЕТАТИВНОЙ ЧАСТИ ТОПИНАМБУРА В СРЕДЕ УКСУСНАЯ КИСЛОТА - ВОДА
Аннотация: The purpose of this work was to evaluate the vegetative part of Jerusalem artichoke as a raw material for the production of cellulose using redox-organosolvent delignification in the medium "CH3СOOН - H2O2 - H2O". The chemical composition of the vegetative part of Jerusalem artichoke and the influence of technological parameters of the delignification process on the yield and properties of the cellulose product were studied. It was found that the vegetative part of Jerusalem artichoke collected in October (stems without leaves) with a chemical composition of more than 50 wt.% of polysaccharides in which presented the Kurshner-Hoffer cellulose more than 90 wt.% was more promising for processing. Experimental methods have been used to determine the parameters of the process of delignification of the vegetative part of Jerusalem artichoke, which provide a high yield of cellulose (42.1 wt.%) with a low content of residual lignin (1.1 wt.%): content of H2O2 - 6.5 wt.%, CH3COOH - 23.4 wt.%, GM 15, duration - 2.5 h, catalyst H2SO4 - 0.2 wt.%. It is shown that pre-extraction of the vegetative part of Jerusalem artichoke with a 0.3% solution of dicarboxylic acids at a temperature of 80 °C with a liquid modulus of 10 for 3 hours reduces the content of residual lignin in the cellulose product to 0.5 wt.% and obtain an extract whose yield is - 7.8 wt.%, including a PB of 4.2 wt.%, which makes it promising for biochemical processing. The obtained results expand the areas of use of the vegetative part of Jerusalem artichoke and show the prospects for further more depth research to optimize the process of delignification and study the properties of the cellulose product. © 2020 Altai State University. All rights reserved.

Scopus

Держатели документа:
Siberian State University of Science and Technology named after Academician M.F. Reshetnev, pr. Krasnoyarskiy rabochiy, 31, Krasnoyarsk, 660037, Russian Federation
Institute of Forest named after V.N. Sukachev SB RAS, Federal Research Center "KSC SB RAS", Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Ryazanova, T. V.; Fedorov, V. S.; Kharyanova, E. V.; Loskutov, S. R.; Kournikova, A. V.

/ O. O. Mamaeva, E. V. Isaeva, S. R. Loskutov, M. A. Plyashechnik // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 1. - С. 277-285, DOI 10.14258/JCPRM.2021018851 . - ISSN 1029-5151
Аннотация: Today, plant waste, including fallen poplar leaves (litter), are a promising raw material for the production of useful prod-ucts using bioconversion, such as protein feed additives. The aim of this study was to study the component composition of the products obtained as a result of the bioconversion of leaf litter. The strain PP-3.2 Pleurotus pulmonarius (Fr.) Quel. In the process of substrate conversion, strain PP-3.2 primarily utilizes extractives and easily hydrolyzable polysaccharides, the amount of which decreases by 44 and 36%, respectively. The total content of polysaccharides is reduced by 20%, lignin substances-9.4%. The loss of substrate mass based on leaf litter was 23%. The product obtained after cultivation contains 28% protein. Analysis of the amino acid composition of the protein showed a high rate of phenylalanine with tyrosine (115), threonine (117.5), valine (110) and isoleucine (105%). Also, the biodegradation product has a high content of glutamic and aspartic acids (11.2 and 25.3% of the total amino acids, respectively). The levels of cadmium, lead and copper revealed as a result of studies do not exceed the maximum permissible concentrations established by the veterinary department. At the same time, there is a high content of iron (166.0 Eg/kg) and zinc (256.7 mg/kg) in the feed product. The digestibility of the product is 54%, the content of nucleic substances is up to 0.3%. Thus, the results obtained show the possibility of using the product of the conversion of poplar fallen leaves as a protein feed additive. © 2021 Altai State University. All rights reserved.

Scopus

Держатели документа:
Reshetnev Siberian State University of Science and Technology, pr. Krasnoyarskii Rabochii, 31, Krasnoyarsk, 660037, Russian Federation
Sukachev Institute of Forest SB RAS, Federal Research Center «Krasnoyarsk Scientific Center SB RAS», Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Mamaeva, O. O.; Isaeva, E. V.; Loskutov, S. R.; Plyashechnik, M. A.

/ T. T. Dao, R. Mikutta, L. Sauheitl [et al.] // J. Geophys. Res.-Biogeosci. - 2022. - Vol. 127, Is. 1. - Ст. e2020JG006181, DOI 10.1029/2020JG006181. - Cited References:122. - Financial support was provided by the German Federal Ministry of Education and Research (03F0616A) within the ERANET EUROPOLAR project CryoCARB. T.T. Dao is grateful for financial support from Vietnamese Education, O. Shibistova acknowledges funding from the National Science Foundation of China and Russian Foundation for Basic Research (NSFC-RFBR joint project No 19-54-53026), and A. Richter, B. Wild and J. Schnecker appreciate the financial support from the Austrian Science Fund (FWF - I370-B17). We thank all members of the CryoCARB project team for their incredible team spirit. We are grateful to the technical staff of the Institute of Soil Science in Hannover for great laboratory assistance. Open access funding enabled and organized by Projekt DEAL. . - ISSN 2169-8953. - ISSN 2169-8961РУБ Environmental Sciences + Geosciences, Multidisciplinary

Аннотация: Permafrost-affected soils in the northern circumpolar region store more than 1,000 Pg soil organic carbon (OC), and are strongly vulnerable to climatic warming. However, the extent to which changing soil environmental conditions with permafrost thaw affects different compounds of soil organic matter (OM) is poorly understood. Here, we assessed the fate of lignin and non-cellulosic carbohydrates in density fractionated soils (light fraction, LF vs. heavy fraction, HF) from three permafrost regions with decreasing continentality, expanding from east to west of northern Siberia (Cherskiy, Logata, Tazovskiy, respectively). In soils at the Tazovskiy site with thicker active layers, the LF showed smaller OC-normalized contents of lignin-derived phenols and plant-derived sugars and a decrease of these compounds with soil depth, while a constant or even increasing trend was observed in soils with shallower active layers (Cherskiy and Logata). Also in the HF, soils at the Tazovskiy site had smaller contents of OC-normalized lignin-derived phenols and plant-derived sugars along with more pronounced indicators of oxidative lignin decomposition and production of microbial-derived sugars. Active layer deepening, thus, likely favors the decomposition of lignin and plant-derived sugars, that is, lignocelluloses, by increasing water drainage and aeration. Our study suggests that climate-induced degradation of permafrost soils may promote carbon losses from lignin and associated polysaccharides by abolishing context-specific preservation mechanisms. However, relations of OC-based lignin-derived phenols and sugars in the HF with mineralogical properties suggest that future OM transformation and carbon losses will be modulated in addition by reactive soil minerals.

WOS

Держатели документа:
Leibniz Univ Hannover, Inst Soil Sci, Hannover, Germany.
Martin Luther Univ Halle Wittenberg, Soil Sci & Soil Protect, Halle, Germany.
VN Sukachev Inst Forest, Krasnoyarsk, Russia.
Univ Vienna, Dept Microbiol & Ecosyst Sci, Vienna, Austria.
Stockholm Univ, Dept Environm Sci & Analyt Chem, Stockholm, Sweden.
Stockholm Univ, Bolin Ctr Climate Res, Stockholm, Sweden.
Univ South Bohemia, Dept Ecosyst Biol, Ceske Budejovice, Czech Republic.
Univ South Bohemia, Fac Sci, Ctr Polar Ecol, Ceske Budejovice, Czech Republic.
Univ Bergen, Ctr Geobiol, Dept Biol, Bergen, Norway.
Ctr Geomicrobiol, Dept Biosci, Aarhus, Denmark.
Russian Acad Sci, Cent Siberian Bot Garden, Siberian Branch, Novosibirsk, Russia.
Ernst Moritz Arndt Univ, Inst Microbiol, Greifswald, Germany.
Austrian Polar Res Inst, Vienna, Austria.

Доп.точки доступа:
Dao, Thao Thi; Mikutta, Robert; Sauheitl, Leopold; Gentsch, Norman; Shibistova, Olga; Wild, Birgit; Schnecker, Joerg; Barta, Jiri; Capek, Petr; Gittel, Antje; Lashchinskiy, Nikolay; Urich, Tim; Santruckova, Hana; Richter, Andreas; Guggenberger, Georg; German Federal Ministry of Education and Research within the ERANET EUROPOLAR project CryoCARB [03F0616A]; Vietnamese Education; National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [19-54-53026]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-54-53026]; Austrian Science FundAustrian Science Fund (FWF) [FWF - I370-B17]; Projekt DEAL

/ V. S. Fedorov, T. V. Ryazanova, Y. A. Litovka [и др.] // J. Sib. Fed. Univ.-Chem. - 2022. - Vol. 15, Is. 1. - С. 90-101, DOI 10.17516/1998-2836-0274. - Cited References:30. - The research was conducted under the governmental task issued by the Ministry of Education and Science of Russia for the "Technology and Equipment for the Chemical Processing of the Plant Biomass Material" project by the Plant Material Deep Conversion Laboratory (topic number FEFE-2020-0016) . . - ISSN 1998-2836. - ISSN 2313-6049РУБ Chemistry, Multidisciplinary

Аннотация: The results of a study on the use of sawdust of common pine Pinus sylvestris L. with their preliminary activation in a cavitation water-impact dispersant for 25 minutes as a substrate for cultivating the G14-16A strain of Ganoderma lucid= are presented. it was found that the G14-16A strain of Ganoderma lucidum colonizes the substrate more efficiently with the introduction of (NH4)(2)SO4 and Na2HPO4 salts into its composition. At the same time, the average growth rate of basidiomycete on the substrate without the introduction of salts was 1.39 +/- 0.53 mm/day, and on the substrate with nitrogen salts - 3.22 +/- 0.48 mm/day, and complete fouling of the substrate occurred on day 13. It was found that during the cultivation of the G14-16A strain of Ganoderma lucidum on a substrate with nitrogen salts, the content of extractive substances in it increases from 4.37 % to 6.32 %, where more than 65 % is accounted for by substances extracted with water. There is a decrease in the content of difficult-to-hydrolyze polysaccharides by 13.3 %, and an increase in the non-hydrolyzable part by 2.4 %, which is 6.8 % per organic mass. The content of easily hydrolyzable polysaccharides (LGA) decreases by 2.4 %, but this change is not significant in terms of organic mass and the share of LGA both before and after bioconversion is about 15.7 %. The obtained data can expand the field of use of sawdust, which is a promising direction, both in the chemical and pharmaceutical industries.

WOS

Держатели документа:
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, Krasnoyarsk, Russia.

Доп.точки доступа:
Fedorov, Vladimir S.; Ryazanova, Tatyana, V; Litovka, Yulia A.; Pavlova, Igor N.; Litvinova, Ekaterina A.; Petrunina, Elena A.; Loskutov, Sergey R.; Ermolin, Vladimir N.; Bayandin, Mikhail A.; Ministry of Education and Science of Russia for the "Technology and Equipment for the Chemical Processing of the Plant Biomass Material" project by the Plant Material Deep Conversion Laboratory [FEFE-2020-0016]

[Текст] / O. A. Shapchenkova, S. R. Loskutov, M. A. Plyashechnik, Z. Pasztory // Сибирский лесной журнал. - 2022. - № 5. - С. 56-69, DOI 10.15372/SJFS20220505 . - ISSN 2311-1410

ГРНТИ

68

Аннотация: Fossil wood that is ca 7 million years old from Bükkábrány (Hungary) was analyzed by thermogravimetry (TG), differential scanning calorimetry (DSC) and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS) to evaluate alterations of its chemical composition. A wood sample of bald cypress ( Taxodium distichum (L.) Rich.) from West Hungary was taken as a reference. The fossil wood was characterized by higher contents of total carbon (58.05 %) and total nitrogen (0.44 %) compared to recent wood. TG of fossil wood showed a high heterogeneity of wood substance, significant loss of polysaccharides and enrichment by lignin including more thermally stable components ( 500 °C). The enthalpy change (∆H) of combustion (thermo-oxidation) for fossil wood was significantly higher than for recent wood (-18.17 kJ/g vs. -11.41 kJ/g). Py-GC/MS analysis of fossil wood showed a significant depletion of polysaccharide pyrolysis products and an increase in lignin pyrolysis products compared to recent wood. The pyrolytic H/L ratio indicates a preferential loss of polysaccharides in fossil wood. Polysaccharide pyrolysis products were rare and represented mainly by levoglucosan. Lignin also underwent substantial changes. A dramatic decrease in monomers, an increase in short side chain compounds and the presence of demethylated/demethoxylated compounds in the composition of lignin pyrolysis products are indicative of lignin alteration (degradation). Moreover, a high abundance of styrene, cresols, phenol and phenolic compounds was observed.

РИНЦ

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Loskutov, Sergey Redzhinal'dovich; Plyashechnik, Mariya Anatol'yevna; Pasztory, Zoltan