: материалы временных коллективов / И. Д. Гродницкая , Н. Д. Сорокин // Лесоведение. - 2010. - № 6. - С. 28-32. - Библиогр. в конце ст.
Аннотация: Полученные экспериментальные данные свидетельствуют о том, что фитопатогенные бактерии родов Pseudomonas, Xanthomonas и Erwinia обнаруживают высокую вирулентность по отношению к семенам и проросткам хвойных только при определенных экологических условиях, когда численность их достигает 10(9) - 10(10) и более ЛЩУ см2 поверхности растений. В других условиях они способны вести себя как ростстимулирующие организмы, повышая иммунитет и снижая гибель сеянцев хвойных на ранних этапах их роста, тем самым способствуя получению устойчивого лесопосадочного материала.
Держатели документа:
Институт леса им. В.Н. Сукачева Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок, 50/28
Доп.точки доступа:
Сорокин, Николай Дмитриевич; Sorokin, Nikolay Dmitriyevich; Grodnitskaya, Irina Dmitriyevna
Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН
w10=
Найдено документов в текущей БД: 13
Application of microbes to the soils of Siberian tree nurseries
[Text] / I. D. Grodnitskaya, N. D. Sorokin // Eurasian Soil Sci. - 2007. - Vol. 40, Is. 3. - P329-334, DOI 10.1134/S106422930703012X. - Cited References: 22
. - 6. - ISSN 1064-2293
РУБ Soil Science
Аннотация: The introduction of Trichoderma viride spores (10(8) CFU per 1 cm(2)) essentially changed the structure of micromycetes in the soils of tree nurseries in Krasnoyarsk region. During the first 20 days, in the variants with dark gray forest soils and podzolized chernozems, the total number of fungi decreased by 3-4 and 1.5 times, respectively, as compared to that in the control plots. During the intense development of the introduced microbes, the species composition of the soil fungi changed considerably. The treatment of Scots pine seeds with metabolites of Trichoderma fungi, as well as Pseudomonas and Bacillus bacteria, in the form of water suspensions, biopreparations, and dry spores promoted an increase in the yield of seedlings and improve their morphometric parameters. At the end of the growing period, the treatment with Trichoderma and the biopreparation on its basis increased these parameters, on average, by 18-70%, and the treatment with bacteria increased the same parameters by 13-15%. The application of microbial preparations improved the phytosanitary state of the soils in the studied tree nurseries. The use of the strains of indigenous microorganisms might be feasible for solving bioremediation problems more successfully in particular regions.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Grodnitskaya, I.D.; Sorokin, N.D.
Аннотация: The introduction of Trichoderma viride spores (10(8) CFU per 1 cm(2)) essentially changed the structure of micromycetes in the soils of tree nurseries in Krasnoyarsk region. During the first 20 days, in the variants with dark gray forest soils and podzolized chernozems, the total number of fungi decreased by 3-4 and 1.5 times, respectively, as compared to that in the control plots. During the intense development of the introduced microbes, the species composition of the soil fungi changed considerably. The treatment of Scots pine seeds with metabolites of Trichoderma fungi, as well as Pseudomonas and Bacillus bacteria, in the form of water suspensions, biopreparations, and dry spores promoted an increase in the yield of seedlings and improve their morphometric parameters. At the end of the growing period, the treatment with Trichoderma and the biopreparation on its basis increased these parameters, on average, by 18-70%, and the treatment with bacteria increased the same parameters by 13-15%. The application of microbial preparations improved the phytosanitary state of the soils in the studied tree nurseries. The use of the strains of indigenous microorganisms might be feasible for solving bioremediation problems more successfully in particular regions.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Grodnitskaya, I.D.; Sorokin, N.D.
Bacterial diseases of conifer seedlings in forest nurseries of central Siberia
[Text] / I. D. Grodnitskaya, A. B. Gukasyan // Microbiology. - 1999. - Vol. 68, Is. 2. - P189-193. - Cited References: 25
. - 5. - ISSN 0026-2617
РУБ Microbiology
Кл.слова (ненормированные):
nurseries -- conifer seedlings -- bacterioses -- phytopathogenic bacteria -- accompanying microflora
Аннотация: In forest nurseries of Siberia, the following diseases of the conifer seedlings were observed: necroses and chloroses of needles (causative agent, Xanthomonas ampelina); bacterial blight of needles and stems (Pseudomonas syringae); vascular bacteriosis (P. solanacearum); blackening and drying of terminal buds, needle bases, and stems (P. fluorescence); tumor formation at the root collar (Agrobacterium radiobacter and A. tumefaciens); wilting and lodging of the young growth due to the injury of the root system (Bacillus mycoides and B. mesentericus).
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forest, Krasnoyarsk, Russia
Доп.точки доступа:
Grodnitskaya, I.D.; Gukasyan, A.B.
Кл.слова (ненормированные):
nurseries -- conifer seedlings -- bacterioses -- phytopathogenic bacteria -- accompanying microflora
Аннотация: In forest nurseries of Siberia, the following diseases of the conifer seedlings were observed: necroses and chloroses of needles (causative agent, Xanthomonas ampelina); bacterial blight of needles and stems (Pseudomonas syringae); vascular bacteriosis (P. solanacearum); blackening and drying of terminal buds, needle bases, and stems (P. fluorescence); tumor formation at the root collar (Agrobacterium radiobacter and A. tumefaciens); wilting and lodging of the young growth due to the injury of the root system (Bacillus mycoides and B. mesentericus).
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forest, Krasnoyarsk, Russia
Доп.точки доступа:
Grodnitskaya, I.D.; Gukasyan, A.B.
Бактериальные заболевания сеянцев хвойных в лесных питомниках Средней Сибири
[Текст] : материалы временных коллективов / И. Д. Гродницкая, А. Б. Гукасян // Микробиология. - 1999. - Т. 68, № 2. - С. 227-231. - Библиогр. в конце ст.
Кл.слова (ненормированные):
питомники -- сеянцы хвойных -- бактериозы -- фитопатогенные бактерии -- сопутствующая микрофлора
Аннотация: В лесных питомниках Сибири отмечены следующие бактериальные заболевания сеянцев хвойных: некрозы и хлорозы хвои (возбудитель - Xanthomonas ampelina); бактериальных ожог хвои и стволиков (возбудитель - Pseudomonas syringae); сосудистый бактериоз (возбудитель - P. solanacearum); почернение и усыхание верхушечных почек и оснований хвои и стволиков (возбудитель - P. fluorescence); образование опухоли у прикорневой шейки (возбудители - Agrobacterium radiobacter и A. tumefaciens); увядание и полегание всходов при поражении корневой системы (возбудители - Bacillus mycoides и B. mesentericus)
ИНФОРМАЦИЯ О ПУБЛИКАЦИИ,
Scopus
Держатели документа:
Институт леса им. В.Н. Сукачева СО РАН : 660036 Красноярск, Академгородок 50, стр. 28
Доп.точки доступа:
Гукасян, А.Б.; Gukasyan, A.B.; Grodnitskaya, Irina Dmitriyevna
Кл.слова (ненормированные):
питомники -- сеянцы хвойных -- бактериозы -- фитопатогенные бактерии -- сопутствующая микрофлора
Аннотация: В лесных питомниках Сибири отмечены следующие бактериальные заболевания сеянцев хвойных: некрозы и хлорозы хвои (возбудитель - Xanthomonas ampelina); бактериальных ожог хвои и стволиков (возбудитель - Pseudomonas syringae); сосудистый бактериоз (возбудитель - P. solanacearum); почернение и усыхание верхушечных почек и оснований хвои и стволиков (возбудитель - P. fluorescence); образование опухоли у прикорневой шейки (возбудители - Agrobacterium radiobacter и A. tumefaciens); увядание и полегание всходов при поражении корневой системы (возбудители - Bacillus mycoides и B. mesentericus)
ИНФОРМАЦИЯ О ПУБЛИКАЦИИ,
Scopus
Держатели документа:
Институт леса им. В.Н. Сукачева СО РАН : 660036 Красноярск, Академгородок 50, стр. 28
Доп.точки доступа:
Гукасян, А.Б.; Gukasyan, A.B.; Grodnitskaya, Irina Dmitriyevna
Microbial complexes of hydromorphic soils in the Selenga River delta (Baikal region)
[Text] / N. D. Sorokin [et al.] // Eurasian Soil Sci. - 2006. - Vol. 39, Is. 7. - P765-770, DOI 10.1134/S1064229306070106. - Cited References: 14
. - 6. - ISSN 1064-2293
РУБ Soil Science
Аннотация: The number and the biomass of microorganisms were determined in the soils of the floodplain and islands in the Selenga River delta. The population of fungi in the soils studied was low. The number of saprotrophic prokaryote microorganisms varied from 10(6)-10(8) CFU/g of soil in the upper horizons to 10(4)-10(5) CFU/g in the lower horizons of the soils. This pattern is typical for most zonal soils. The microbial biomass in the floodplain soils was 2-4 times as high as that in the soils of the islands. The number of microorganisms of different ecologic-trophic groups participating in the nitrogen and carbon mobilization was much lower than that in the hydromorphic soils of the Transbaikal region or in the cryogenic soils of the Angara River basin (Irkutsk district). The low coefficient of microbiological mineralization and the low coefficient showing the lack of nitrogen (coefficient of oligotrophness) in the soils indicated the weak processes of organic matter decomposition in the soils studied. During the season investigated (August-September), the bacterial complexes in all the soils were dominated by bacteria of the genera Bacillus, Pseudomonas, and Aquaspirillum. In the floodplain soils, streptomycetes constituted a considerable part of the microbial complexes of the floodplain soils, whereas, in the soils of the islands, their number was minor.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Div, Inst Gen & Expt Biol, Ulan Ude 670047, Russia
Доп.точки доступа:
Sorokin, N.D.; Makushkin, E.O.; Korsunov, V.M.; Afanasova, E.N.; Shakhmatova, E.Y.
Аннотация: The number and the biomass of microorganisms were determined in the soils of the floodplain and islands in the Selenga River delta. The population of fungi in the soils studied was low. The number of saprotrophic prokaryote microorganisms varied from 10(6)-10(8) CFU/g of soil in the upper horizons to 10(4)-10(5) CFU/g in the lower horizons of the soils. This pattern is typical for most zonal soils. The microbial biomass in the floodplain soils was 2-4 times as high as that in the soils of the islands. The number of microorganisms of different ecologic-trophic groups participating in the nitrogen and carbon mobilization was much lower than that in the hydromorphic soils of the Transbaikal region or in the cryogenic soils of the Angara River basin (Irkutsk district). The low coefficient of microbiological mineralization and the low coefficient showing the lack of nitrogen (coefficient of oligotrophness) in the soils indicated the weak processes of organic matter decomposition in the soils studied. During the season investigated (August-September), the bacterial complexes in all the soils were dominated by bacteria of the genera Bacillus, Pseudomonas, and Aquaspirillum. In the floodplain soils, streptomycetes constituted a considerable part of the microbial complexes of the floodplain soils, whereas, in the soils of the islands, their number was minor.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Div, Inst Gen & Expt Biol, Ulan Ude 670047, Russia
Доп.точки доступа:
Sorokin, N.D.; Makushkin, E.O.; Korsunov, V.M.; Afanasova, E.N.; Shakhmatova, E.Y.
Patterns of formation of microbial communities of alluvial soils in the Selenga estuary
[Text] / E. O. Makushkin [et al.] // Biol. Bull. - 2005. - Vol. 32, Is. 5. - P450-455, DOI 10.1007/s10525-005-0123-1. - Cited References: 20
. - 6. - ISSN 1062-3590
РУБ Biology
Аннотация: Microbial communities of alluvial soils in the facies on islands in the Selenga estuary and near Stelynoi Dvorets and Istomino Settlements were studied. The distribution of the CFU-dominant microorganisms (Bacillus and Pseudomonas + Arthrobacter) has been revealed in the studied island soils. In addition, actinomycetes Streptomyces were found among the dominants in soils of the near-terrace floodplain. The quantitative distribution of microorganisms across soil layers was demonstrated. The effect of ecological factors in formation of microbial communities on the islands and central floodplain regions was revealed.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Inst Gen & Expt Biol, Siberian Div, Ulan Ude 670047, Russia
Russian Acad Sci, Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Makushkin, E.O.; Sorokin, N.D.; Korsunov, V.M.; Shakhmatova, E.Y.; Afanasova, E.N.
Аннотация: Microbial communities of alluvial soils in the facies on islands in the Selenga estuary and near Stelynoi Dvorets and Istomino Settlements were studied. The distribution of the CFU-dominant microorganisms (Bacillus and Pseudomonas + Arthrobacter) has been revealed in the studied island soils. In addition, actinomycetes Streptomyces were found among the dominants in soils of the near-terrace floodplain. The quantitative distribution of microorganisms across soil layers was demonstrated. The effect of ecological factors in formation of microbial communities on the islands and central floodplain regions was revealed.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Inst Gen & Expt Biol, Siberian Div, Ulan Ude 670047, Russia
Russian Acad Sci, Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Makushkin, E.O.; Sorokin, N.D.; Korsunov, V.M.; Shakhmatova, E.Y.; Afanasova, E.N.
Tree species and moisture effects on soil sources of N2O: Quantifying contributions from nitrification and denitrification with O-18 isotopes
[Text] / O. V. Menyailo, B. A. Hungate // J. Geophys. Res.-Biogeosci. - 2006. - Vol. 111, Is. G2. - Ст. G02022, DOI 10.1029/2005JG000058. - Cited References: 36
. - 8. - ISSN 0148-0227
РУБ Environmental Sciences + Geosciences, Multidisciplinary
Аннотация: Nitrous oxide (N2O) is an important greenhouse gas and participates in the destruction of stratospheric ozone. Soil bacteria produce N2O through denitrification and nitrification, but these processes differ radically in substrate requirements and responses to the environment. Understanding the controls over N2O efflux from soils, and how N2O emissions may change with climate warming and altered precipitation, require quantifying the relative contributions from these groups of soil bacteria to the total N2O flux. Here we used ammonium nitrate (NH4NO3, including substrates for both processes) in which the nitrate has been enriched in the stable isotope of oxygen, O-18, to partition microbial sources of N2O, arguing that a molecule of N2O carrying the O-18 labeled will have been produced by denitrification. We compared the influences of six common tree species on the relative contributions of nitrification and denitrification to N2O flux from soils, using soils from the Siberian afforestation experiment. We also altered soil water content, to test whether denitrification becomes a dominant source of N2O when soil water content increases. Tree species altered the proportion of nitrifier and denitrifier-derived N2O. Wetter soils produced more N2O from denitrification, though the magnitude of this effect varied among tree species. This indicates that the roles of denitrification and nitrification vary with tree species, and, that tree species influence soil responses to increased water content.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, SB RAS, Inst Forest, Krasnoyarsk, Russia
No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA
No Arizona Univ, Merriam Powell Ctr Environm Res, Flagstaff, AZ 86011 USA
Доп.точки доступа:
Menyailo, O.V.; Hungate, B.A.
Рубрики:
ARTIFICIAL AFFORESTATION EXPERIMENT
CONTAINING NITRITE REDUCTASE
NITROUS-OXIDE
DENITRIFYING BACTERIA
STABLE-ISOTOPE
NITRIFIER DENITRIFICATION
NITROSOMONAS-EUROPAEA
PSEUDOMONAS-STUTZERI
OXYGEN
WATER
ARTIFICIAL AFFORESTATION EXPERIMENT
CONTAINING NITRITE REDUCTASE
NITROUS-OXIDE
DENITRIFYING BACTERIA
STABLE-ISOTOPE
NITRIFIER DENITRIFICATION
NITROSOMONAS-EUROPAEA
PSEUDOMONAS-STUTZERI
OXYGEN
WATER
Аннотация: Nitrous oxide (N2O) is an important greenhouse gas and participates in the destruction of stratospheric ozone. Soil bacteria produce N2O through denitrification and nitrification, but these processes differ radically in substrate requirements and responses to the environment. Understanding the controls over N2O efflux from soils, and how N2O emissions may change with climate warming and altered precipitation, require quantifying the relative contributions from these groups of soil bacteria to the total N2O flux. Here we used ammonium nitrate (NH4NO3, including substrates for both processes) in which the nitrate has been enriched in the stable isotope of oxygen, O-18, to partition microbial sources of N2O, arguing that a molecule of N2O carrying the O-18 labeled will have been produced by denitrification. We compared the influences of six common tree species on the relative contributions of nitrification and denitrification to N2O flux from soils, using soils from the Siberian afforestation experiment. We also altered soil water content, to test whether denitrification becomes a dominant source of N2O when soil water content increases. Tree species altered the proportion of nitrifier and denitrifier-derived N2O. Wetter soils produced more N2O from denitrification, though the magnitude of this effect varied among tree species. This indicates that the roles of denitrification and nitrification vary with tree species, and, that tree species influence soil responses to increased water content.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, SB RAS, Inst Forest, Krasnoyarsk, Russia
No Arizona Univ, Dept Biol Sci, Flagstaff, AZ 86011 USA
No Arizona Univ, Merriam Powell Ctr Environm Res, Flagstaff, AZ 86011 USA
Доп.точки доступа:
Menyailo, O.V.; Hungate, B.A.
НЕКОТОРЫЕ ОСОБЕННОСТИ ФОРМИРОВАНИЯ МИКРОБНЫХ СООБЩЕСТВ В ПОЙМЕННЫХ ПОЧВАХ ДЕЛЬТЫ РЕКИ СЕЛЕНГА
[Текст] : материалы временных коллективов / Э. О. Макушкин [и др.] // Известия РАН. Серия биологическая. - 2005. - № 5. - С. 545-551. - Библиогр. в конце ст.
Аннотация: Исследовались микробные сообщества пойменных почв ряда фаций, расположенных на островах дельты р. Селенга и около сел Степной Дворец и Истомино. Выявлено распределение доминирующих КОЕ микроорганизмов: Bacillus и Pseudomonas + Arthrobacter для исследуемых почв островов. В почвах притеррасной поймы среди доминант представлены еще актиномицеты Streptomyces. Показано численное распределение микроорганизмов по горизонтам почв. Выявлено влияние экологических особенностей в формировании микробных сообществ на островах и на участках центральной поймы.
ИНФОРМАЦИЯ О ПУБЛИКАЦИИ
Держатели документа:
Институт Леса им. В.Н. Сукачева СО РАН : 660036 Красноярск, Академгородок 50/28
Доп.точки доступа:
Макушкин, Эдуард Очирович; Makushkin E.O.; Сорокин, Николай Дмитриевич; Sorokin, Nikolay Dmitriyevich; Корсунов, Владимир Михайлович; Korsunov Vladimir Mikhailovich; Шахматова, Е.Ю.; Shakhmatova Ye.Yu.; Афанасова, Елена Николаевна; Afanasova Yelena Nikolayevna
Аннотация: Исследовались микробные сообщества пойменных почв ряда фаций, расположенных на островах дельты р. Селенга и около сел Степной Дворец и Истомино. Выявлено распределение доминирующих КОЕ микроорганизмов: Bacillus и Pseudomonas + Arthrobacter для исследуемых почв островов. В почвах притеррасной поймы среди доминант представлены еще актиномицеты Streptomyces. Показано численное распределение микроорганизмов по горизонтам почв. Выявлено влияние экологических особенностей в формировании микробных сообществ на островах и на участках центральной поймы.
ИНФОРМАЦИЯ О ПУБЛИКАЦИИ
Держатели документа:
Институт Леса им. В.Н. Сукачева СО РАН : 660036 Красноярск, Академгородок 50/28
Доп.точки доступа:
Макушкин, Эдуард Очирович; Makushkin E.O.; Сорокин, Николай Дмитриевич; Sorokin, Nikolay Dmitriyevich; Корсунов, Владимир Михайлович; Korsunov Vladimir Mikhailovich; Шахматова, Е.Ю.; Shakhmatova Ye.Yu.; Афанасова, Елена Николаевна; Afanasova Yelena Nikolayevna
Использование микробного антагонизма в защите сеянцев хвойных от инфекционных заболеваний в лесных питомниках
[Текст] : материалы временных коллективов / И. Д. Гродницкая, О. Э. Кондакова // Известия Санкт-Петербургской лесотехнической академии. - Санкт-Петербург : СПбГЛТА, 2014. - Вып. 207. - С. 154-163. - Библиогр. в конце ст.
Кл.слова (ненормированные):
защита сеянцев хвойных -- микробный антагонизм -- фитопатогенные микромицеты -- инфекционные заболевания
Аннотация: Из почв лесных питомников Сибири выделены и испытаны штаммы микроорганизмов, обладающие высокой биологической (антагонистической, ростстимулирующей) активностью. Внесенные в почвы лесопитомников бактерии родов Bacillus, Pseudomonas и грибы р. Trichoderma существенно снижали численность фитопатогенных микромицетов (роды Fusarium, Alternaria, Cladosporium), улучшали фитосанитарное состояние почв питомников. Предпосевная обработка семян сосны обыкновенной и лиственницы сибирской микробами-антагонистами повышала их грунтовую всхожесть (в 3,5–5,8 раз) и увеличивала сохранность сеянцев (в 4,5–12,0 раз) к концу вегетации, по сравнению с контролем.
Полный текст
Доп.точки доступа:
Кондакова, Оксана Эриковна; Kondakova, O. E; Grodnitskaya, Irina Dmitriyevna
Кл.слова (ненормированные):
защита сеянцев хвойных -- микробный антагонизм -- фитопатогенные микромицеты -- инфекционные заболевания
Аннотация: Из почв лесных питомников Сибири выделены и испытаны штаммы микроорганизмов, обладающие высокой биологической (антагонистической, ростстимулирующей) активностью. Внесенные в почвы лесопитомников бактерии родов Bacillus, Pseudomonas и грибы р. Trichoderma существенно снижали численность фитопатогенных микромицетов (роды Fusarium, Alternaria, Cladosporium), улучшали фитосанитарное состояние почв питомников. Предпосевная обработка семян сосны обыкновенной и лиственницы сибирской микробами-антагонистами повышала их грунтовую всхожесть (в 3,5–5,8 раз) и увеличивала сохранность сеянцев (в 4,5–12,0 раз) к концу вегетации, по сравнению с контролем.
Полный текст
Доп.точки доступа:
Кондакова, Оксана Эриковна; Kondakova, O. E; Grodnitskaya, Irina Dmitriyevna
Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
/ S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180
. - ISSN 0045-6535
Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation
Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.
Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation
Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.
Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards "green" bioplastics
/ S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180. - Cited References:101. - This study (polymer synthesis and investigation) was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning", and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (investigation of polymer degradation in soils of Evenkia).
. - ISSN 0045-6535. - ISSN 1879-1298
РУБ Environmental Sciences
Кл.слова (ненормированные):
Siberian cryogenic soils -- P(3HB) bioplastic -- metabolic activity -- structure of microbial community -- P(3HB)-degrading strains -- P(3HB) -- properties
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, VN Sukachev Inst Forest, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Melnikov Permafrost Inst, 36 Merzlotnaya St, Yakutsk 677010, Russia.
Доп.точки доступа:
Prudnikova, Svetlana, V; Evgrafova, Svetlana Yu; Volova, Tatiana G.; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Кл.слова (ненормированные):
Siberian cryogenic soils -- P(3HB) bioplastic -- metabolic activity -- structure of microbial community -- P(3HB)-degrading strains -- P(3HB) -- properties
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, VN Sukachev Inst Forest, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Melnikov Permafrost Inst, 36 Merzlotnaya St, Yakutsk 677010, Russia.
Доп.точки доступа:
Prudnikova, Svetlana, V; Evgrafova, Svetlana Yu; Volova, Tatiana G.; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Antimicrobial and anti-radical activity of individual fractions of Pinus sibirica du tour and Abies sibirica Ledeb., native to the Siberian region
/ A. A. Efremov, I. D. Zykova, V. A. Senashova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 203-210, DOI 10.14258/JCPRM.2020047505
. - ISSN 1029-5151
Перевод заглавия: АНТИМИКРОБНАЯ И АНТИРАДИКАЛЬНАЯ АКТИВНОСТЬ ОТДЕЛЬНЫХ ФРАКЦИЙ PINUS SIBIRICA DU TOUR И ABIES SIBIRICA LEDEB., ПРОИЗРАСТАЮЩИХ В СИБИРСКОМ РЕГИОНЕ
Кл.слова (ненормированные):
2,2-diphenyl-1-picrilhydrazyl -- Antimicrobial activity -- Antiradical activity -- Essential oil -- Siberian fir (Abies sibirica Ledeb.) -- Siberian pine (Pinus sibirica Du Tour)
Аннотация: By the method of exhaustive hydroparodistillation, essential oil was obtained from Siberian pine (Pinus sibirica Du Tour) and Siberian fir (Abies sibirica Ledeb.), growing on the territory of the Krasnoyarsk territory. Separate fractions of oil were obtained: the first one after 45 min from the beginning of distillation, the second - after 2 hours, the third - after 5 hours, the fourth-after 10 hours, the fifth fraction was collected after the end of hydrodistillation. The antimicrobial activity of separate fractions of essential oil of P. sibirica and A. sibirica was studied against strains of opportunistic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus 209p, Micrococcus luteus, Acinetobacter baumanii, Candida albicans. The performed studies showed that all the studied samples of essential oils showed either bactericidal or bacteriostatic activity against the strains of microorganisms taken in the experiment, except Pseudomonas aeruginosa. The type of activity displayed depended on the type of strain and sample of essential oil. It is noted that the sensitivity of the experimental strains to the components of the essential oils of P. sibirica and A. sibirica decreases during the transition from the first to the last fraction. We assume that, apparently, this is due to a decrease in the number of monoterpenes in the composition of oils.To study the antiradical activity, the reaction of essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. Antiradical activity of both whole essential oils of P. sibirica and A. sibirica and their separate fractions was established. There was an increase in antiradical activity with a decrease in the content of monoterpenes in the composition of essential oil. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Siberian Federal University, Svobodny pr., 79, Krasnoyarsk, 660049, Russian Federation
Special Design and Technology Bureau “Nauka, The Federal Research Center of KNC SB RAS, Akademgorodok, 50, p. 45, Krasnoyarsk, 660036, Russian Federation
Institute of Forest. V. N. Sukacheva Federal Research Center KNC SB RAS, Akademgorodok, 50, p. 28, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Efremov, A. A.; Zykova, I. D.; Senashova, V. A.; Grodnickaya, I. D.; Pashenova, N. V.
Перевод заглавия: АНТИМИКРОБНАЯ И АНТИРАДИКАЛЬНАЯ АКТИВНОСТЬ ОТДЕЛЬНЫХ ФРАКЦИЙ PINUS SIBIRICA DU TOUR И ABIES SIBIRICA LEDEB., ПРОИЗРАСТАЮЩИХ В СИБИРСКОМ РЕГИОНЕ
Кл.слова (ненормированные):
2,2-diphenyl-1-picrilhydrazyl -- Antimicrobial activity -- Antiradical activity -- Essential oil -- Siberian fir (Abies sibirica Ledeb.) -- Siberian pine (Pinus sibirica Du Tour)
Аннотация: By the method of exhaustive hydroparodistillation, essential oil was obtained from Siberian pine (Pinus sibirica Du Tour) and Siberian fir (Abies sibirica Ledeb.), growing on the territory of the Krasnoyarsk territory. Separate fractions of oil were obtained: the first one after 45 min from the beginning of distillation, the second - after 2 hours, the third - after 5 hours, the fourth-after 10 hours, the fifth fraction was collected after the end of hydrodistillation. The antimicrobial activity of separate fractions of essential oil of P. sibirica and A. sibirica was studied against strains of opportunistic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus 209p, Micrococcus luteus, Acinetobacter baumanii, Candida albicans. The performed studies showed that all the studied samples of essential oils showed either bactericidal or bacteriostatic activity against the strains of microorganisms taken in the experiment, except Pseudomonas aeruginosa. The type of activity displayed depended on the type of strain and sample of essential oil. It is noted that the sensitivity of the experimental strains to the components of the essential oils of P. sibirica and A. sibirica decreases during the transition from the first to the last fraction. We assume that, apparently, this is due to a decrease in the number of monoterpenes in the composition of oils.To study the antiradical activity, the reaction of essential oil components with a stable free 2,2-diphenyl-1-picrylhydrazyl radical was used. Antiradical activity of both whole essential oils of P. sibirica and A. sibirica and their separate fractions was established. There was an increase in antiradical activity with a decrease in the content of monoterpenes in the composition of essential oil. © 2020 Altai State University. All rights reserved.
Scopus
Держатели документа:
Siberian Federal University, Svobodny pr., 79, Krasnoyarsk, 660049, Russian Federation
Special Design and Technology Bureau “Nauka, The Federal Research Center of KNC SB RAS, Akademgorodok, 50, p. 45, Krasnoyarsk, 660036, Russian Federation
Institute of Forest. V. N. Sukacheva Federal Research Center KNC SB RAS, Akademgorodok, 50, p. 28, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Efremov, A. A.; Zykova, I. D.; Senashova, V. A.; Grodnickaya, I. D.; Pashenova, N. V.
Antimicrobial and Antiradical Activities of Individual Fractions of Pinus sibirica Du Tour and Abies sibirica Ledeb. Growing in Siberia
/ A. A. Efremov, I. D. Zykova, V. A. Senashova [et al.] // Russ. J. Bioorg. Chem. - 2021. - Vol. 47, Is. 7. - P1439-1444, DOI 10.1134/S1068162021070062. - Cited References:22
. - ISSN 1068-1620. - ISSN 1608-330X
РУБ Biochemistry & Molecular Biology + Chemistry, Organic
Аннотация: Essential oil from the spruce branches of Siberian pine (Pinus sibirica Du Tour) and Siberian fir (Abies sibirica Ledeb.) growing on the territory of Krasnoyarsk krai has been obtained by exhaustive hydrosteam distillation. Five separate oil fractions have been extracted: the first was collected 45 min after the onset of distillation; the second, at 2 h; the third, at 5 h; the fourth, at 6 h after the onset of distillation, and the fifth fraction was collected after the termination of hydro-steam distillation. The antimicrobial activity of separate fractions of essential oil from P sibirica and A. sibirica toward the strains of conditionally pathogenic bacteria has been tested: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus 209p, Micrococcus luteus, Acinetobacter baumanii, and Candida albicans. The results of the study have shown that all samples of essential oils exhibit either bactericidal or bacteriostatic activity toward microbial strains used in the experiment except for Pseudomonas aeruginosa. The type of activity depends on the microbial strain and the sample of essential oil. It has been noted that the sensitivity of the strains to the components of P sibirica and A. sibirica essential oils diminishes on going from the first to the last fraction. It has been assumed that this is due to a decrease in the content of monoterpenes in the oil composition. The antiradical activity has been examined by the reaction of essential oil components with the stable free 2,2-diphenyl-1-picrylhydrazyl radical. It has been found that both whole essential oils from P sibirica and A. sibirica and their individual fractions exhibit antiradical activity. It has been noted that the antiradical activity increases with decreasing content of monoterpenes in the composition of the essential oil.
WOS
Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660049, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Special Design & Technol Bur Nauka, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Sukhachev Forest Inst, Akademgorodok 660036, Russia.
Доп.точки доступа:
Efremov, A. A.; Zykova, I. D.; Senashova, V. A.; Grodnitckaya, I. D.; Pashenova, N., V
Рубрики:
ESSENTIAL OILS
ANTIOXIDANT
Кл.слова (ненормированные):
Siberian pine (Pinus sibirica Du Tour) -- Siberian fir (Abies sibirica -- Ledeb.) -- individual fractions of essential oil -- antimicrobial activity -- antiradical activity -- 2,2-diphenyl-1-picrylhydrazyl
ESSENTIAL OILS
ANTIOXIDANT
Кл.слова (ненормированные):
Siberian pine (Pinus sibirica Du Tour) -- Siberian fir (Abies sibirica -- Ledeb.) -- individual fractions of essential oil -- antimicrobial activity -- antiradical activity -- 2,2-diphenyl-1-picrylhydrazyl
Аннотация: Essential oil from the spruce branches of Siberian pine (Pinus sibirica Du Tour) and Siberian fir (Abies sibirica Ledeb.) growing on the territory of Krasnoyarsk krai has been obtained by exhaustive hydrosteam distillation. Five separate oil fractions have been extracted: the first was collected 45 min after the onset of distillation; the second, at 2 h; the third, at 5 h; the fourth, at 6 h after the onset of distillation, and the fifth fraction was collected after the termination of hydro-steam distillation. The antimicrobial activity of separate fractions of essential oil from P sibirica and A. sibirica toward the strains of conditionally pathogenic bacteria has been tested: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus 209p, Micrococcus luteus, Acinetobacter baumanii, and Candida albicans. The results of the study have shown that all samples of essential oils exhibit either bactericidal or bacteriostatic activity toward microbial strains used in the experiment except for Pseudomonas aeruginosa. The type of activity depends on the microbial strain and the sample of essential oil. It has been noted that the sensitivity of the strains to the components of P sibirica and A. sibirica essential oils diminishes on going from the first to the last fraction. It has been assumed that this is due to a decrease in the content of monoterpenes in the oil composition. The antiradical activity has been examined by the reaction of essential oil components with the stable free 2,2-diphenyl-1-picrylhydrazyl radical. It has been found that both whole essential oils from P sibirica and A. sibirica and their individual fractions exhibit antiradical activity. It has been noted that the antiradical activity increases with decreasing content of monoterpenes in the composition of the essential oil.
WOS
Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660049, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Special Design & Technol Bur Nauka, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Sukhachev Forest Inst, Akademgorodok 660036, Russia.
Доп.точки доступа:
Efremov, A. A.; Zykova, I. D.; Senashova, V. A.; Grodnitckaya, I. D.; Pashenova, N., V