Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / V. L. Semerikov [et al.] // Russ. J. Genet. - 2015. - Vol. 51, Is. 12. - P1199-1203, DOI 10.1134/S1022795415120108. - Cited References:20. - We thank Y.Y. Hhrunyk, A. I. Vidjakin, V.V. Tarakanov, E.V. Hantemirova, and I.V. Tikhonova for assistance with the pine material collection. The study was supported by Russian Foundation for Basic Research (grant 13-04-01028) and by Russian Federation Government (grant 14.Y26.31.0004). . - ISSN 1022-7954. - ISSN 1608-3369РУБ Genetics & Heredity

Аннотация: Fragments of genomic DNA of Scots pine (Pinus sylvestris L.) homologous to the mitochondrial DNA (mtDNA) contigs of Norway spruce (Picea abies (L.) Karst.) and loblolly pine (Pinus taeda L.) were resequenced in a sample of the Scots pine trees of European, Siberian, Mongolian, and Caucasian origin in order to develop mtDNA markers. Flanking non-coding regions of some mitochondrial genes were also investigated and resequenced. Five single nucleotide polymorphisms (SNPs) and a single minisatellite locus were identified. Caucasian samples differed from the rest by three SNPs. Two SNPs have been linked to an early described marker in the first intron of the nad7 gene, and all together revealed three haplotypes in European populations. No variable SNPs were found in the Siberian and Mongolian populations. The minisatellite locus contained 41 alleles across European, Siberian, and Mongolian populations, but, this locus demonstrated a weak population differentiation (F (ST) = 5.8), probably due to its high mutation rate.

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Держатели документа:
Russian Acad Sci, Ural Branch, Inst Plant & Anim Ecol, Ekaterinburg 620144, Russia.
Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Univ Gottingen, D-37077 Gottingen, Germany.
Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 119991, Russia.
Texas A&M Univ, College Stn, TX 77843 USA.

Доп.точки доступа:
Semerikov, V. L.; Putintseva, Yu. A.; Oreshkova, N. V.; Semerikova, S. A.; Krutovsky, K. V.; Russian Foundation for Basic Research [13-04-01028]; Russian Federation Government [14.Y26.31.0004]

/ E. I. Bondar [et al.] // BMC Bioinformatics. - 2019. - Vol. 20: 11th International Multiconference on Bioinformatics of Genome (AUG 20-25, 2018, Novosibirsk, RUSSIA). - Ст. 38, DOI 10.1186/s12859-018-2571-x. - Cited References:25. - The presented study was a part of the project "Genomic studies of major boreal coniferous forest tree species and their most dangerous pathogens in the Russian Federation" funded by the Government of the Russian Federation (grant No 14.Y26.31.0004). Publication costs are funded by the BioMed Central Membership of the University of Gottingen. . - ISSN 1471-2105РУБ Biochemical Research Methods + Biotechnology & Applied Microbiology

Аннотация: BackgroundThe main objectives of this study were sequencing, assembling, and annotation of chloroplast genome of one of the main Siberian boreal forest tree conifer species Siberian larch (Larix sibirica Ledeb.) and detection of polymorphic genetic markers - microsatellite loci or simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs).ResultsWe used thedata of the whole genome sequencing of three Siberian larch trees from different regions - theUrals, Krasnoyarsk, and Khakassia, respectively. Sequence reads were obtained using the Illumina HiSeq2000 in the Laboratory of Forest Genomics at the Genome Research and Education Center ofthe Siberian Federal University. The assembling was done using the Bowtie2 mapping program and the SPAdes genomic assembler. The genome annotation was performed using the RAST service. We used the GMATo program for the SSRs search, and the Bowtie2 and UGENE programs for the SNPs detection. Length of the assembled chloroplast genome was 122,561bp, which is similar to 122,474bp in the closely related European larch (Larix decidua Mill.). As a result of annotation and comparison of the data with theexisting data available only for three larch species - L. decidua, L. potaninii var. chinensis (complete genome 122,492bp), and L. occidentalis (partial genome of 119,680bp), we identified 110 genes, 34 of which represented tRNA, 4 rRNA, and 72 protein-coding genes. In total, 13 SNPs were detected; two of them were in the tRNA-Arg and Cell division protein FtsH genes, respectively. In addition, 23 SSR loci were identified.ConclusionsThe complete chloroplast genome sequence was obtained for Siberian larch for the first time. The reference complete chloroplast genomes, such as one described here, would greatly help in the chloroplast resequencing and search for additional genetic markers using population samples. The results of this research will be useful for further phylogenetic and gene flow studies in conifers.

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Держатели документа:
Siberian Fed Univ, Genome Res & Educ Ctr, Lab Forest Genom, Krasnoyarsk 660036, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, Lab Forest Genet & Select, Siberian Branch, Krasnoyarsk 660036, Russia.
Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany.
Russian Acad Sci, Vavilov Inst Gen Genet, Lab Populat Genet, Moscow 119333, Russia.
Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA.

Доп.точки доступа:
Bondar, Eugeniya I.; Putintseva, Yuliya A.; Oreshkova, Nataliya V.; Krutovsky, Konstantin V.; Krutovsky, Konstantin; Government of the Russian Federation [14.Y26.31.0004]; University of Gottingen

[Текст] : научное издание / С. В. Новикова, Н. В. Орешкова, В. В. Шаров, В. Л. Семериков, К. В. Крутовский // Сибирский экологический журнал. - 2023. - Т. 30, № 5. - С. 675-691, DOI 10.15372/SEJ20230509 . - ISSN 0869-8619
   Перевод заглавия: GENETIC STRUCTURE AND GEOGRAPHICAL DIFFERENTIATION OF SIBERIAN LARCH (LARIX SIBIRICA LEDEB.) POPULATIONS BASED ON GENOME GENOTYPING BY SEQUENCING

ГРНТИ

68.47.03

Аннотация: Приведены результаты исследования генетической дифференциации популяций лиственницы сибирской (Larix sibirica Ledeb.) в широтном градиенте климатических условий, полученные на основе генотипирования генома с помощью высокопроизводительного секвенирования геномных районов ДНК, ассоциированных с сайтами рестрикции (ddRADseq). Изучена также корреляция пяти основных климатических переменных с изменчивостью 47 929 генетических маркеров - однонуклеотидных полиморфизмов или “снипов” (от английского SNPs - single nucleotide polymorphisms). Всего изучено 125 деревьев: 61 дерево в четырех популяциях вдоль западной географической трансекты и 64 дерева в четырех популяциях вдоль восточной географической трансекты. Выявлен 21 SNPs с признаками отбора, включая 9 SNPs аутлайеров, чья изменчивость не может быть объяснена селективно-нейтральными процессами, и 12 SNPs, чья изменчивость коррелировала с изменчивостью некоторых климатических факторов. Семь SNPs расположены в интронах митохондриальных генов, три расположены вблизи митохондриальных генов, кодирующих NAD2 и рибосомальные белки S7 и S11, один на отдалении от ядерного гена, кодирующего белок, гомологичный связанному с микротрубочками futsch-подобному белку Arabidopsis thaliana, два в белковых генах неизвестной природы и три в контигах, не содержащих гены, и для которых не найдены гомологичные последовательности в NCBI GenBank.
The genetic differentiation of Siberian larch (Larix sibirica Ledeb.) populations in the latitudinal gradient of climatic conditions was studied based on high-throughput double digest restriction-site associated DNA sequencing (ddRADseq) data. We studied the correlation of five main climatic variables with the variability of 47,929 single nucleotide polymorphisms (SNPs). A total of 125 trees were studied: 61 trees in four populations along the western geographic transect and 64 trees in four populations along the eastern geographic transect. 21 SNPs with signatures of selection were identified, including 9 outlier SNPs whose variability cannot be explained by selectively neutral processes, and 12 SNPs whose variability correlated with the environmental factors. Seven SNPs are located in the introns of mitochondrial genes, three are located relatively close to the mitochondrial genes encoding NAD2 and ribosomal proteins S7 and S11, one is located at a distance from the nuclear gene encoding a protein homologous to the microtubule-associated futsch-like protein of Arabidopsis thaliana, two in the protein genes of an unknown nature and three in contigs containing no genes, and for which no homologous sequences were found in the NCBI GenBank.

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Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Орешкова, Наталья Викторовна; Шаров, В.В.; Семериков, В.Л.; Semerikov V.L.; Крутовский, Константин Валерьевич; Krutovsky, Konstantin V.