: материалы временных коллективов / А. С. Сиренко // Исследования компонентов лесных экосистем Сибири: Материалы конференции молодых ученых 3-4 марта 2009 г. - Красноярск : Институт леса им. В.Н. Сукачева СО РАН , 2009. - Вып. 10. - С. 54-57. - Библиогр. в конце ст.
Аннотация: Воздействие метаболитов на начальной стадии развития не оказало стимулирующего влияния на изолированные зародыши и рост каллусных культур сосны сибирской на индукционной среде.
Держатели документа:
Институт леса им. В.Н. Сукачева Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок 50/28
Доп.точки доступа:
Sirenko A.S.
Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН
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Найдено документов в текущей БД: 6
Сокультивирование изолированных зародышей и эмбриогенных каллусов сосны сибирской с метаболитами штаммов рода Trichoderma и Streptomyces
Patterns of formation of microbial communities of alluvial soils in the Selenga estuary
[Text] / E. O. Makushkin [et al.] // Biol. Bull. - 2005. - Vol. 32, Is. 5. - P450-455, DOI 10.1007/s10525-005-0123-1. - Cited References: 20
. - 6. - ISSN 1062-3590
РУБ Biology
Аннотация: Microbial communities of alluvial soils in the facies on islands in the Selenga estuary and near Stelynoi Dvorets and Istomino Settlements were studied. The distribution of the CFU-dominant microorganisms (Bacillus and Pseudomonas + Arthrobacter) has been revealed in the studied island soils. In addition, actinomycetes Streptomyces were found among the dominants in soils of the near-terrace floodplain. The quantitative distribution of microorganisms across soil layers was demonstrated. The effect of ecological factors in formation of microbial communities on the islands and central floodplain regions was revealed.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Inst Gen & Expt Biol, Siberian Div, Ulan Ude 670047, Russia
Russian Acad Sci, Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Makushkin, E.O.; Sorokin, N.D.; Korsunov, V.M.; Shakhmatova, E.Y.; Afanasova, E.N.
Аннотация: Microbial communities of alluvial soils in the facies on islands in the Selenga estuary and near Stelynoi Dvorets and Istomino Settlements were studied. The distribution of the CFU-dominant microorganisms (Bacillus and Pseudomonas + Arthrobacter) has been revealed in the studied island soils. In addition, actinomycetes Streptomyces were found among the dominants in soils of the near-terrace floodplain. The quantitative distribution of microorganisms across soil layers was demonstrated. The effect of ecological factors in formation of microbial communities on the islands and central floodplain regions was revealed.
Полный текст,
WOS,
Scopus
Держатели документа:
Russian Acad Sci, Inst Gen & Expt Biol, Siberian Div, Ulan Ude 670047, Russia
Russian Acad Sci, Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Makushkin, E.O.; Sorokin, N.D.; Korsunov, V.M.; Shakhmatova, E.Y.; Afanasova, E.N.
НЕКОТОРЫЕ ОСОБЕННОСТИ ФОРМИРОВАНИЯ МИКРОБНЫХ СООБЩЕСТВ В ПОЙМЕННЫХ ПОЧВАХ ДЕЛЬТЫ РЕКИ СЕЛЕНГА
[Текст] : материалы временных коллективов / Э. О. Макушкин [и др.] // Известия РАН. Серия биологическая. - 2005. - № 5. - С. 545-551. - Библиогр. в конце ст.
Аннотация: Исследовались микробные сообщества пойменных почв ряда фаций, расположенных на островах дельты р. Селенга и около сел Степной Дворец и Истомино. Выявлено распределение доминирующих КОЕ микроорганизмов: Bacillus и Pseudomonas + Arthrobacter для исследуемых почв островов. В почвах притеррасной поймы среди доминант представлены еще актиномицеты Streptomyces. Показано численное распределение микроорганизмов по горизонтам почв. Выявлено влияние экологических особенностей в формировании микробных сообществ на островах и на участках центральной поймы.
ИНФОРМАЦИЯ О ПУБЛИКАЦИИ
Держатели документа:
Институт Леса им. В.Н. Сукачева СО РАН : 660036 Красноярск, Академгородок 50/28
Доп.точки доступа:
Макушкин, Эдуард Очирович; Makushkin E.O.; Сорокин, Николай Дмитриевич; Sorokin, Nikolay Dmitriyevich; Корсунов, Владимир Михайлович; Korsunov Vladimir Mikhailovich; Шахматова, Е.Ю.; Shakhmatova Ye.Yu.; Афанасова, Елена Николаевна; Afanasova Yelena Nikolayevna
Аннотация: Исследовались микробные сообщества пойменных почв ряда фаций, расположенных на островах дельты р. Селенга и около сел Степной Дворец и Истомино. Выявлено распределение доминирующих КОЕ микроорганизмов: Bacillus и Pseudomonas + Arthrobacter для исследуемых почв островов. В почвах притеррасной поймы среди доминант представлены еще актиномицеты Streptomyces. Показано численное распределение микроорганизмов по горизонтам почв. Выявлено влияние экологических особенностей в формировании микробных сообществ на островах и на участках центральной поймы.
ИНФОРМАЦИЯ О ПУБЛИКАЦИИ
Держатели документа:
Институт Леса им. В.Н. Сукачева СО РАН : 660036 Красноярск, Академгородок 50/28
Доп.точки доступа:
Макушкин, Эдуард Очирович; Makushkin E.O.; Сорокин, Николай Дмитриевич; Sorokin, Nikolay Dmitriyevich; Корсунов, Владимир Михайлович; Korsunov Vladimir Mikhailovich; Шахматова, Е.Ю.; Shakhmatova Ye.Yu.; Афанасова, Елена Николаевна; Afanasova Yelena Nikolayevna
Biological activity assessment of museum cultures of antagonist microorganisms and their use for presowing treatment of Scots pine seeds (Pinus sylvestris L.) in vitro
/ O. E. Kondakova, I. D. Grodnitskaya // Vestn. Tomsk. Gos. Univ. Biol. - 2018. - Is. 42. - С. 54-68, DOI 10.17223/19988591/42/3
. - ISSN 1998-8591
Кл.слова (ненормированные):
Antagonistic and enzymatic activity -- Bacillus -- Growthpromoting effect -- Phytopathogens -- Streptomyces -- Trichoderma
Аннотация: The microbiological method is applied for the purpose of artificial forest regeneration, as the most effective method of protecting forest planting material grown in forest nurseries. At present, literature data contain many examples of using species and genera of microorganisms belonging to different taxa in order to protect plants. The aim of the research was to establish biological (antagonistic, enzymatic and growth-stimulating) activity of the museum microorganism cultures belonging to different taxonomic groups (bacteria, fungi), and to assess their influence on the growth and development of Scots pine seeds in vitro and a decrease in the number of phytopathogenic fungi. We isolated previously selected microorganisms from the nursery soils; these microorganisms belong to different taxonomic groups, namely, Trichoderma micromycetes (T. harzianum, T. longibrachaitum, and T. lignorum), Streptomyces lateritius bacteria, Bacillus amyloliquefaciens, as well as phytopathogenic Fusarium fungi (F. oxysporum, F. moniliforme, F. proliferatum, F. moniliforme var annullatum, and F. oxysporum B3). Antagonistic activity of microbial strains was determined by the dual culture method, and the presence of enzymatic activity (lipase, proteinase and chitinase) of the tested strains was observed by qualitative express tests. We studied the growth-promoting activity by soaking pine seeds in aqueous suspensions of antagonists (106 spores/ml) (Pegalado, 2000; Cullimore, 2001; Montealegre, 2003; Asaturova, 2012). The results of the research showed that the investigated microorganisms (fungi, actinobacteria and bacteria) are biologically active. The most powerful antagonists were micromycetes of T. harzianum, T. lignorum, and T. longibrachiatum, which are also capable of exhibiting mycophilic properties (hyperparasitism). Thus, T. longibrachiatum showed mycophilia against three strains: F. moniliforme, F. moniliforme var annulatum, and F. oxysporum B3, whereas T. harzianum and T. lignorum did against two: F. moniliforme and F. proliferatum; the degree of phytopathogen inhibition (ID) varied from 30 to 100% (See Table 1). The strain of B. amiloliquefaciens bacterium was less active, the DI was 41.4%, on the average, and the slowest antagonistic properties were exhibited by actinobacterium S. lateritius - 14.8%, on the average. The investigation of the presence of the main hydrolytic enzymes (a hitinaze, a lipase, protease) showed that Trichoderma micromycetes had the average and strong hydrolytic activity (T. harzianum and T. longibrachiatum), and bacteria (S. lateritius, B. amyloliquefaciens) had the average and weak hydrolytic activity (See Table 2). Also, all the investigated strains improved Scots pine seed germination, while the strains of B. amyloliquefaciens and T. longibrachiatum showed the greatest growth-promoting activity (See Figures). Thus, we found that the investigated strains (T. harzianum, T. lignorum, T. longibrachiatum, S. lateritius, and B. amyloliquefaciens) had a high antagonistic activity, and Trichoderma micromycetes revealed the ability for mycoparasitism. The high biological (enzymatic, antagonistic, growth-stimulating) activity of the studied strains of microorganisms makes them effective agents for biological control in forest nurseries. © 2018 Tomsk State University. All rights reserved.
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Держатели документа:
Laboratory of Microbiology and Ecological Biotechnology, VN Sukachev Institute of Forest, Siberian Branch of the Russian Academy of Sciences, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Kondakova, O. E.; Grodnitskaya, I. D.
Кл.слова (ненормированные):
Antagonistic and enzymatic activity -- Bacillus -- Growthpromoting effect -- Phytopathogens -- Streptomyces -- Trichoderma
Аннотация: The microbiological method is applied for the purpose of artificial forest regeneration, as the most effective method of protecting forest planting material grown in forest nurseries. At present, literature data contain many examples of using species and genera of microorganisms belonging to different taxa in order to protect plants. The aim of the research was to establish biological (antagonistic, enzymatic and growth-stimulating) activity of the museum microorganism cultures belonging to different taxonomic groups (bacteria, fungi), and to assess their influence on the growth and development of Scots pine seeds in vitro and a decrease in the number of phytopathogenic fungi. We isolated previously selected microorganisms from the nursery soils; these microorganisms belong to different taxonomic groups, namely, Trichoderma micromycetes (T. harzianum, T. longibrachaitum, and T. lignorum), Streptomyces lateritius bacteria, Bacillus amyloliquefaciens, as well as phytopathogenic Fusarium fungi (F. oxysporum, F. moniliforme, F. proliferatum, F. moniliforme var annullatum, and F. oxysporum B3). Antagonistic activity of microbial strains was determined by the dual culture method, and the presence of enzymatic activity (lipase, proteinase and chitinase) of the tested strains was observed by qualitative express tests. We studied the growth-promoting activity by soaking pine seeds in aqueous suspensions of antagonists (106 spores/ml) (Pegalado, 2000; Cullimore, 2001; Montealegre, 2003; Asaturova, 2012). The results of the research showed that the investigated microorganisms (fungi, actinobacteria and bacteria) are biologically active. The most powerful antagonists were micromycetes of T. harzianum, T. lignorum, and T. longibrachiatum, which are also capable of exhibiting mycophilic properties (hyperparasitism). Thus, T. longibrachiatum showed mycophilia against three strains: F. moniliforme, F. moniliforme var annulatum, and F. oxysporum B3, whereas T. harzianum and T. lignorum did against two: F. moniliforme and F. proliferatum; the degree of phytopathogen inhibition (ID) varied from 30 to 100% (See Table 1). The strain of B. amiloliquefaciens bacterium was less active, the DI was 41.4%, on the average, and the slowest antagonistic properties were exhibited by actinobacterium S. lateritius - 14.8%, on the average. The investigation of the presence of the main hydrolytic enzymes (a hitinaze, a lipase, protease) showed that Trichoderma micromycetes had the average and strong hydrolytic activity (T. harzianum and T. longibrachiatum), and bacteria (S. lateritius, B. amyloliquefaciens) had the average and weak hydrolytic activity (See Table 2). Also, all the investigated strains improved Scots pine seed germination, while the strains of B. amyloliquefaciens and T. longibrachiatum showed the greatest growth-promoting activity (See Figures). Thus, we found that the investigated strains (T. harzianum, T. lignorum, T. longibrachiatum, S. lateritius, and B. amyloliquefaciens) had a high antagonistic activity, and Trichoderma micromycetes revealed the ability for mycoparasitism. The high biological (enzymatic, antagonistic, growth-stimulating) activity of the studied strains of microorganisms makes them effective agents for biological control in forest nurseries. © 2018 Tomsk State University. All rights reserved.
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Смотреть статью
Держатели документа:
Laboratory of Microbiology and Ecological Biotechnology, VN Sukachev Institute of Forest, Siberian Branch of the Russian Academy of Sciences, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Доп.точки доступа:
Kondakova, O. E.; Grodnitskaya, I. D.
Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
/ S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180
. - ISSN 0045-6535
Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation
Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.
Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation
Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.
Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards "green" bioplastics
/ S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180. - Cited References:101. - This study (polymer synthesis and investigation) was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning", and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (investigation of polymer degradation in soils of Evenkia).
. - ISSN 0045-6535. - ISSN 1879-1298
РУБ Environmental Sciences
Кл.слова (ненормированные):
Siberian cryogenic soils -- P(3HB) bioplastic -- metabolic activity -- structure of microbial community -- P(3HB)-degrading strains -- P(3HB) -- properties
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, VN Sukachev Inst Forest, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Melnikov Permafrost Inst, 36 Merzlotnaya St, Yakutsk 677010, Russia.
Доп.точки доступа:
Prudnikova, Svetlana, V; Evgrafova, Svetlana Yu; Volova, Tatiana G.; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Кл.слова (ненормированные):
Siberian cryogenic soils -- P(3HB) bioplastic -- metabolic activity -- structure of microbial community -- P(3HB)-degrading strains -- P(3HB) -- properties
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
WOS
Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, VN Sukachev Inst Forest, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Melnikov Permafrost Inst, 36 Merzlotnaya St, Yakutsk 677010, Russia.
Доп.точки доступа:
Prudnikova, Svetlana, V; Evgrafova, Svetlana Yu; Volova, Tatiana G.; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]