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    Application of Toxigenic Alternaria oxytropis to Soybeans and its Effect on Swainsonine Detection in Different Environments
/ O. Moodley [et al.] // Bull. Environ. Contam. Toxicol. - 2018, DOI 10.1007/s00128-018-2496-0 . - Article in press. - ISSN 0007-4861

Кл.слова (ненормированные):
Alkaloid toxin -- Alternaria oxytropis -- Locoweeds -- Soybean -- Swainsonine -- Toxin detection -- Algae -- Amino acids -- Nitrogen fixation -- Polysaccharides -- Soils -- Alternaria -- Locoweeds -- Soybean -- Swainsonine -- Toxin detection -- Toxic materials

Аннотация: Alternaria oxytropis is an endophytic fungus of locoweeds that synthesizes swainsonine toxin. In this work, we evaluated the effect of A. oxytropis on soybean seedlings and quantified swainsonine in different culture conditions. Soybean (Glycine max) seeds were co-cultured with A. oxytropis (at different concentrations of mycelial suspensions) in agar media and soil culture, and swainsonine was assayed using LC–MS/MS. The results showed evidence that A. oxytropis infected soybean seedlings produced detectable swainsonine in agar culture while the toxin was undetectable or below the detection limit (0.006% of swainsonine dry weight) in soil media even at higher concentrations of the fungus. These results suggest that swainsonine detection is highly dependent on culture conditions and that soybeans co-cultured with A. oxytropis in soil could potentially be used to limit toxin production. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.

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Держатели документа:
Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun, Jilin Province 130118, China
South African Department of Agriculture, Forestry and Fisheries (DAFF), Pretoria, 0001, South Africa
Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan
V.N. Sukachev Institute of Forest SB RAS, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Moodley, O.; Sun, Y.; Sossah, F. L.; Kakishima, M.; Pavlov, I. N.; Li, Y.; Wang, Q.