Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

: материалы временных коллективов / A. P. Abaimov [и др.] // Proceedings of the eighth symposium on the joint Siberian permafrost studies between Japan and Russia in 1999. - Onogawa : National Institute for Environmental Studies, 2000. - С. 3-9. - рус. - Библиогр. в конце ст.
Аннотация: The obtained experimental data testify that even in the case of stands mortality as a result of ground fires it is right to count on quite successful natural regeneration of Gmelin larch on burned areas. It is right to consider genetically conditioned ability of Larix gmelini to conserve a part of quality seeds in ripe cones for 3-4 years as an adaptive reaction of the species to periodical wildfire impacts which are the main destabilizing factor in high latitudes of real zone in Eurasia.

Держатели документа:
Институт леса им. В.Н. Сукачева Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок, 50 стр. 28

Доп.точки доступа:
Abaimov, Anatoly Platonovich ; Абаимов Анатолий Платонович; Erkalov, A.V.; Еркалов А.В.; Prokushkin, Staniclav Grigor'evich; Прокушкин Станислав Григорьевич; Matsuura, Y.; Матсуура У.

: материалы временных коллективов / G. V. Kuznetsova // Eurasian Journal of Forest Research. - 2008. - Vol. 11-2. - С. 81-87. - Библиогр. в конце ст.
Аннотация: Experiments in intraspecific hybridization of Siberian stone pine, not just of this species but of different regional origins as well, have been carried out at a clone plantation in Middle Siberia (Krasnoyars forest-steppe). Crossings were realized using the principle of ecologo-geographical remoteness of populations. Siberian stone pine clones chosen for crossing had good growth and constant reproductive ability over many years. An analysis of the characteristics of hybrid female cones (weight, linear size, number of developed scales) and seeds (number, weight, seed fullness, viability) showed the positive influence of controlled pollination with combinations of different climatypes. The study of hybrid growing climatypes, resulting from crossing of the plain and mountain populations, also revealed signs of heterosis.

Держатели документа:
Институт леса им. В.Н. Сукачева Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок, 50, стр., 28

Доп.точки доступа:
Кузнецова, Галина Васильевна

= Охрана македонской сосны PINUS PEUCE GRISEB. в Македонии : материалы временных коллективов / Н. Христовская [и др.] // Сохранение лесных генетических ресурсов Сибири: материалы 3-го Международного совещания (23-29 августа 2011, Красноярск, Россия). - С. 155-156
Аннотация: In modern classification of pine rimiliyskaya refers to subsection STROBI LOD, inside thje section STROBI of the subgenus STROBUS (Haplohylon) or white pine. Feature this subsection is: the presence of a conductive vascular bundle inside the needles, needles are collected in bundles of five needles, the ledge on the scales of female cones are located closer to the outer edge, largre seeds with the impeller. The mature female cones are easy to open, releasing the seeds. The wood can grow up to 40 m high with piramidal habitus. There is five needles 7-10 sm long and 0,75 sm wide greygreen colour.

Держатели документа:
Институт леса им. В.Н. Сукачева Сибирского отделения Российской академии наук : 660036, Красноярск, Академгородок 50/28

Доп.точки доступа:
Христовская, Н.; Khristovskaya N.; Блажекович, И.; Blazhekovich I.; Настевска, И.; Nastevska I.; Кузнецова, Галина Васильевна; Kuznetsova, Galina Vasil'yevna

/ V. V. Ermolenko [et al.] // Contemp. Probl. Ecol. - 2013. - Vol. 6, Is. 2. - P242-246, DOI 10.1134/S1995425513020030. - Cited References: 12 . - 5. - ISSN 1995-4255РУБ Ecology

Аннотация: The sowing qualities of the seeds of Abies sibirica Ledeb. from trees of I to V Kraft grades and the total content of nitrogen in the seeds were determined. The best qualities are found in the seeds taken from the middle part of the cones growing in the upper part of crowns of trees of I and II Kraft grades. The nitrogen content in the seeds varies within the range 2.7-3.46 mg/g and depends on the growth and development of the trees. The correlation between these characteristics was found to be 0.81-0.94.

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Держатели документа:
[Ermolenko, V. V.
Prokushkin, S. G.
Zubareva, O. N.] Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia
[Erokhina, Z. V.] Siberian State Technol Univ, Krasnoyarsk 660049, Russia

Доп.точки доступа:
Ermolenko, V.V.; Prokushkin, S.G.; Zubareva, O.N.; Erokhina, Z.V.

[Text] / N. A. Kuz'mina // Russ. J. Ecol. - 2004. - Vol. 35, Is. 5. - P303-307, DOI 10.1023/B:RUSE.0000040683.10024.5e. - Cited References: 24 . - 5. - ISSN 1067-4136РУБ Ecology

Аннотация: Taxonometric parameters and morphological characters of cones and seeds were studied in larch trees growing in uneven-aged larch forests of the green-moss and herbaceous types. Significant differences in trunk volume and the number of cones in the crown was revealed. Differences in the parameters of cones and the quality of seeds proved to be insignificant. Correlations between the taxonometric parameters of trees and the morphological characters of cones and seeds were analyzed.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Siberian Div, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Kuz'mina, N.A.

[Text] / I. N. Tretyakova, E. V. Bazhina // Ekol. Bratisl. - 2000. - Vol. 19, Is. 3. - P280-294. - Cited References: 49 . - 15. - ISSN 1335-342XРУБ Ecology

Аннотация: Structure of crown and viability of generative organs of Siberian fir were studied in disturbed forest ecosystems near Baikal Lake. The crown structure changes in damaged trees: a zone of subapical drying appears and apical dominance is lost. Needle chlorosis and necrosis appear and proceed in sprouts of male sexualization. The tree top including the female generative organs does not show degradation symptoms during about 30 years and produces seed cones. Processes of degradation in male generative zone greatly influence on fir tree pollen. Pollen viability of fir trees varied from year to year in different trees but always pollen quality of trees growing in the Khamar-Daban Mts in the air pollution zone was more poor in comparison with the pollen of trees at die Baikal Lake shore. Tree pollen growing in the Khamar-Daban Mts germinates in vitro but pollen tubes are formed short and not viable. Seed quality of fir trees is very low, A lot of seeds are sterile. Empty seeds prevail. The formation of empty seeds was caused by the low pollen germination in ovules and as a whole absence of pollen in ovules. The high sterility of generative organs and especially of Abies sibirica pollen can limit gametic selection and decrease fir diversity in forest ecosystems near Baikal Lake.

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Держатели документа:
RAS, SB, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Tretyakova, I.N.; Bazhina, E.V.

/ I. V. Tikhonova [et al.] // Contemp. Probl. Ecol. - 2014. - Vol. 7, Is. 1. - P60-66, DOI 10.1134/S1995425514010156 . - ISSN 1995-4255
Аннотация: Studies of the phenotypic variability of cones and seeds of trees in terms of five index and six qualitative traits in the populations of Scots pine in the south of Krasnoyarsk krai, Khakassia, Tuva, and Buryatia are carried out using techniques of A.I. Vidyakin (2004). The population level of seven traits and validity of their use as markers of genetic variability in Southern Siberian pine populations is confirmed. В© 2014 Pleiades Publishing, Ltd.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Tikhonova, I.V.; Tarakanov, V.V.; Tikhonova, N.A.; Barchenkov, A.P.; Ekart, A.K.

[Text] / I. N. Tret'yakova, E. V. Voroshilova, D. N. Shuvaev // Russ. J. Plant Physiol. - 2014. - Vol. 61, Is. 2. - P274-280, DOI 10.1134/S1021443714020162. - Cited References: 24. - This work was supported by the Russian Foundation for Basic Research, project no. 11-04-00281-a; and a r-Sibir'-a research grant, project no. 13-04-98045. . - ISSN 1021-4437. - ISSN 1608-3407РУБ Plant Sciences

Аннотация: The results of long-term work on the induction of somatic embryogenesis in Siberian pine (Pinus sibirica Du Tour) growing in a natural stand of trees and in clone grafting plantation located in the Western Sayan are shown. Controlled pollination of the clones of Siberian pine had a positive influence on the state of callus cultures. The cytological analysis of embryonal-suspensor mass made it possible to identify embryological structures morphologically close to zygotic embryos at early developmental stages; as a result, the callus tissue was recognized embryogenic. We revealed donor plants (clones), whose zygotic embryos in vitro can serve as a source of embryogenic callus tissue.

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Держатели документа:
[Tret'yakova, I. N.
Voroshilova, E. V.
Shuvaev, D. N.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia
ИЛ СО РАН

Доп.точки доступа:
Tret'yakova, I.N.; Voroshilova, E.V.; Shuvaev, D.N.; Russian Foundation for Basic Research [11-04-00281-a]; a r-Sibir'-a research grant [13-04-98045]

/ V. P. Vetrova, A. P. Barchenkov, Y. V. Savenkova // Contemp. Probl. Ecol. - 2014. - Vol. 7, Is. 2. - P192-203, DOI 10.1134/S1995425514020164 . - ISSN 1995-4263
Аннотация: The assessment of ecogeographic variability and differentiation of 22 cenopopulations of Japanese dwarf pine Pinus pumila (Pall.) Regel (Pinaceae) on the Kamchatka Peninsula and in Koryakia has been carried out in regard to morphological and phenotypic characters of mature seed cones. The viability of seeds in the cones of ten populations has been determined by a radiographic method. A latitudinal nature of phenotypic differentiation of cenopopulations in the region has been revealed. Lower seed viability related to a high percent of seeds without embryos or with underdeveloped embryos has been recorded in the northeastern populations. The average share of viable seeds in the region is 52% and in the northeastern populations it reaches only 6-24%. © 2014 Pleiades Publishing, Ltd.

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Держатели документа:
Kamchatka Branch of the Pacific Geographical Institute, Far East Branch, Russian Academy of Sciences, pr. Rybakov 19-A, Petropavlovsk-Kamchatsky, 683024, Russian Federation
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Vetrova, V.P.; Barchenkov, A.P.; Savenkova, Y.V.

/ V. P. Vetrova [et al.] // Russ. J. Genet. Appl. Res. - 2016. - Vol. 6, Is. 5. - P560-568, DOI 10.1134/S2079059716050142 . - ISSN 2079-0597
Аннотация: The structures of Picea ajanensis populations were compared based on the allozyme analysis of vegetative buds and the morphometric analysis of generative organs. Six cenopopulations of P. ajanensis were investigated in areas with various levels of volcanic impact in the Kamchatka Peninsula. The genetic structures of spruce populations and phenotypes were determined by the analysis of ten enzyme systems (PGM, GOT, HK, LAP, MDH, SKDH, IDH, GDH, PGI, and SOD). The phenotypic variability of spruce populations was estimated based on the composition of morphotypes that were identified by using the geometric morphometrics of cone-scale shapes. Pairwise comparison of samples of cones from 170 trees from six populations revealed 12 morphotypes differing in the shape of the cone scales. Comparative assessment of the variability and similarity of the populations was carried out based on the frequency of the occurrence of the phenotypes and the frequency of the alleles of the polymorphic loci. Correlations of the genetic and phenotypic distance matrices between different phenotypes were revealed. This observation was consistent with the genetic determination of the shape of the cone scales in spruce. Genetic differences between the morphotypes with regard to nine polymorphic loci (Got-2, Skdh-1, Idh-2, Pgm-2, Mdh-1, Mdh-3, Pgm-1, Pgi-2, and Hk) were not significant. Statistically significant differences between the spruce morphotypes were revealed for two loci: Pgm-2 and Mdh-1. Differences in the genetic diversity of spruce populations generally corresponded to differences in their phenotypic diversity. The high levels of genetic and phenotypic diversity characterized a stable population structure of spruce in the area of weak volcanic influence. Changes in the genetic structure and low levels of the phenotypic diversity of spruce were observed under catastrophic volcanic impact. © 2016, Pleiades Publishing, Ltd.

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Держатели документа:
Kamchatka Branch, Pacific Geographical Institute, Far East Branch, Russian Academy of Sciences, Petropavlovsk-Kamchatsky, Russian Federation
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vetrova, V. P.; Ekart, A. K.; Kravchenko, A. N.; Larionova, A. Y.

/ V. P. Vetrova, N. V. Sinelnikova, A. P. Barchenkov // Turczaninowia. - 2018. - Vol. 21, Is. 2. - С. 86-100, DOI 10.14258/turczaninowia.21.2.10 . - ISSN 1560-7259
Аннотация: Intra- A nd interspecifc variability of three larch species of North Asia (Larix cajanderi, L. dahurica, L. sibirica) has been analyzed based on the shape of cone scales, using geometric morphometrics. L. cajanderi was represented by nine population samples of cones from three regions: Kamchatka, Yakutia, and Magadan Oblast. L. cajanderi cones were compared with L. dahurica cones from Evenkia and the Transbaikal region. Samples of L. sibirica cones were collected in three regions of South Siberia. Patterns of shape variation of cone-scales were investigated using principal component analysis (PCA) of partial warps of scales. The PCA-values (relative warps) were used as the features of shape of scales in conducting discriminant analysis. The study revealed similar main trends in variability of cone scale shapes, suggesting parallel variability of generative organs in Larix species of North Asia. The Mahalonobis (D2) distances calculated from relative warps of scales vary from 14.7 to 25 when comparing the populations of L. sibirica and L. cajanderi. Between populations of closely related species, L. dahurica and L. cajanderi, D2 vary from 6.4 to 15.5. Between populations of L. sibirica and L. dahurica, D2 is 13.8-21.4 for the sample L. dahurica from Evenkia and 7.6-12.2 for the Transbaikal one. The shape of the cone scales of L. cajanderi differs from that of L. dahurica, proving that this is an independent species. The high degree of differentiation between the populations of L. cajanderi in Yakutia and Kamchatka indicates that they may belong to different geographical races. © 2018 Altai State University. All rights reserved.

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Держатели документа:
Kamchatka Branch, Pacifc Geographical Institute, Far East Branch, Russian Academy of Sciences, Rybakov str., 19-a, Petropavlovsk-Kamchatsky, Russian Federation
Institute of Biological Problems of the North, Far-Eastern Branch, Russian Academy of Sciences, Portovaya str., 18, Magadan, Russian Federation
V. N. Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/28, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vetrova, V. P.; Sinelnikova, N. V.; Barchenkov, A. P.

[Текст] : статья / В. И. ВОРОНИН, А. П. СОФРОНОВ, Т. И. МОРОЗОВА [и др.] // География и природные ресурсы. - 2019. - № 4. - С. 56-65 . - ISSN 0206-1619
   Перевод заглавия: IN DARK-CONIFEROUS FORESTS ON KHAMAR-DABAN RANGE (SOUTHERN CISBAIKALIA)

УДК

911.2

528.9

632.3

630.443.3

630*561.24

Аннотация: Приводятся данные комплексного исследования причин и масштабов усыхания темнохвойных лесов в геосистемах северного макросклона хр. Хамар-Дабан с 2006 по 2009 г. Причиной усыхания стало заболевание «бактериальная во дянка», вызываемое бактерией Erwinia nimipressuralis Carter. Спусковым механизмом для развития заболевания по служило маловодье, наблюдавшееся в регионе в этот период. Для оценки масштабов повреждения темнохвойных лесов использовался ландшафтный подход. Была создана среднемасштабная карта геосистем северного макросклона хр. Ха мар-Дабан, на которой показано разнообразие геосистем региона уровня классов фаций. Выполнена работа по ранжи рованию лесов по трем степеням пораженности древостоя (от сильной до слабой) и проведена оценка территории их распространения. Основные площади нарушенных лесов сосредоточены в восточной и западной областях Хамар-Даба на, где они занимают среднюю и верхнюю полосы горно-таежного пояса. Наиболее пострадал от заболевания кедровый древостой. Пихта повреждена меньше, но в ряде мест нарушение кедрового и пихтового древостоя сопоставимо. Усыхание елового древостоя не выявлено. Резкое падение скорости прироста у кедрового древостоя приходится на 2006-2009 гг. Кроме этого, отмечается уменьшение содержания питательных веществ в пыльцевых зернах кедра и снижение активности их прорастания, а также негативная трансформация кедровых шишек в виде их избыточного засмоления и недоразвитости, что является одним из диагностических признаков бактериальной водянки. Нарушение развития пыльцы и шишек кедра негативно сказывается на вызревании семян кедра, замедляя лесовозобновление. Однако изучение состояния подроста в пострадавших от бактериальной водянки насаждениях свидетельствует об удовлетворительных в целом процессах восстановления темнохвойных лесов.
Presented are the data from a comprehensive investigation into the causes and extent of dark coniferous forest dieback in geosystems of the northern macroslope of Khamar-Daban Range for the time interval 2006-2009. Forest dieback was caused by bacteria Erwinia nimipressuralis Carter. The disease outbreak was triggered by water scarcity in the region at that period. Land scape approach was used in assessing the magnitude of damage to dark-coniferous forests. The medium-scale map of geosystems was created for the northern macroslope of Khamar-Daban Range, showing the region’s geosystem diversity at the level of classes of facies. The ranking of forests was done according to three degrees of damage to tree stands (from strong to weak), and an assessment was made of the territory covered by them. The main areas of affected forests are concentrated in the eastern and western regions of Khamar-Daban where they occupy the middle and upper parts of the mountain-taiga belt. The Siberian stone pine stands suffered the most from the disease. Fir trees were less affected; in some areas, however, the damage to Siberian stone pine and fir stands is comparable. A dramatic decrease of the rate of radial increment in Siberian stone pine stands corresponds to the time interval 2006-2009. Furthermore, there occurred a decrease in nutrient content in pollen grains of Siberian stone pine and a decrease in of the rate of their germination as well as a negative transformation of Siberian stone pine cones in the form of their excessive resinosis and immaturity, which is one of the diagnostic features of bacterial dropsy. A disturbance to the development of pollen and pine cones negatively affects the ripening of Siberian stone pine seeds thus impeding forest regenera tion. However, a study of the state of the young growth in forest stands affected by bacterial dropsy bears witness to the gener ally satisfactory processes of dark-coniferous forest rehabilitation.

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Держатели документа:
Институт географии им. В.Б. Сочавы СО РАН
Институт леса им. В.Н. Сукачёва СО РАН
Красноярский научный центр СО РАН
Педагогический институт Иркутского государственного университета
Сибирский институт физиологии и биохимии растений СО РАН

Доп.точки доступа:
ВОРОНИН, В.И.; VORONIN V.I.; СОФРОНОВ, А.П.; SOFRONOV A.P.; МОРОЗОВА, Т.И.; MOROZOVA T.I.; ОСКОЛКОВ, В.А.; OSKOLKOV V.A.; Суховольский, Владислав Григорьевич; Soukhovolsky Vladislav Grigor'yevich; КОВАЛЁВ, А.В.; KOVALEV A.V.

/ V. P. Vetrova, A. P. Barchenkov, N. V. Sinelnikova // Vestn. Tomsk. Gos. Univ. Biol. - 2021. - Is. 53. - С. 47-67, DOI 10.17223/19988591/53/3. - Cited References:38 . - ISSN 1998-8591. - ISSN 2311-2077РУБ Biology + Ecology

Аннотация: Geometric morphometric analysis of shape variation in the cone scales of two closely related larch species, Larix dahurica Laws. (=Larix gmelinii (Rupr.) Rupr) and L. cajanderi Mayr, was carried out. The data on the taxonomy and distribution of L. dahurica and L. cajanderi are contradictory. The taxonomic status of L. cajanderi has been confirmed by the genetic and morphological studies performed in Russia and based on considerable evidence, but the species has not been recognized internationally, being considered as a synonym of Larix gmelinii var. gmelinii. In the systematics of larch, morphological characters of the generative organs are mainly used as diagnostic markers, among the most important being the shape variation of the cone scales. The aim of this study was to test geometric morphometrics as a tool for analyzing differentiation of L. dahurica and L. cajanderi in the shape of their cone scales. Characterization of shape variations in cone scales using geometric morphometric methods consists in digitizing points along an outline of scales followed by analysis of partial warps, describing individual differences in coordinates of the outline points. We studied the populations of L. dahurica from Evenkia and the Trans-Baikal region and six L. cajanderi populations from Yakutia and Magadan Oblast. In each population, we analyzed samples of 100-150 cones collected from 20-30 trees. Scales taken from the middle part of the cones were scanned using an Epson Perfection V500 Photo. On the scanned images, outline points were placed with a TPSDig program (Rolf, 2010), using angular algorithm (Oreshkova et al., 2015). The data were processed and analyzed using Integrated Morphometrics Programs (IMP) software (http://www.canisius.edu/similar to sheets/morphsoft.html, Sheets, 2001), following the guidelines on geometric morphometrics in biology (Pavlinov, Mikeshina, 2002; Zelditch et al., 2004). Initial coordinates of the scale landmarks were aligned with the mean structure for L. dahurica and L. cajanderi cone scales using Procrustes superimposition in the CoordGen6 program. PCA based on covariances of partial warp scores was applied to reveal directions of variation in the shape of the cone scales. The relative deformations of the cone scales (PCA scores) were used as shape variables for statistical comparisons of these two larch species with canonical discriminant analysis. Morphotypes of the cone scales were distinguished in L. dahurica populations by pairwise comparison of samples from trees in the TwoGroup6h program using Bootstrap resampling-based Goodall's F-test (Sheets, 2001). Samples from the trees in which the cone scales differed significantly (p 0.01) were considered to belong to different morphotypes. Morphotypes distinguished in L. dahurica populations were compared with the morphotypes that we had previously determined in L. cajanderi populations. The composition and the frequency of occurrence of morphotypes were used to determine phenotypic distances between populations (Zhivotovskii, 1991). Multidimensional scaling matrix of the phenotypic distances was applied for ordination of larch populations. In this research, we revealed differentiation of L. dahurica and L. cajanderi using geometric morphometric analysis of the shape variation of cone scales. The results of PCA of partial warp scores exposed four principal components, which account for 90% of total explained variance in the shape of the cone scales in the two larch species. Graphical representations of these shape transformations in the vector form characterized directions of shape variability in scales corresponding to the maximum and minimum values of four principal components (See Fig. 2). PCA-ordination of the larch populations revealed some difference in the shape variation of the cone scales in L. dahurica and L. cajanderi (See Fig. 3). The results of canonical discriminant analysis of relative deformations of scales showed differentiation of the populations of the two larch species (See Fig. 4). Eleven morphotypes were identified in L. dahurica cones from Evenkia and nine morphotypes in the Ingoda population, three of the morphotypes being common for both populations (See Fig. 5). The shape of L. dahurica cone scales varied from spatulate to oval and their apical margins from weakly sinuate to distinctly sinuate. The Trans-Baikal population was dominated by scales with obtuse (truncate) and rounded apexes. The obtained morphotypes were compared with 25 cone scale morphotypes previously distinguished in the Yakut and the Magadan L. cajanderi populations (See Fig. 3). Four similar morphotypes of cone scales were revealed in the North-Yeniseisk population of L. dahurica and the Yakut populations of L. cajanderi. The differences between them in the populations of the two larch species were nonsignificant (p 0.01). All morphotypes of cone scales from the Ingoda population of L. dahurica differed significantly from L. cajanderi cone scale morphotypes. The results of multidimensional scaling phenotypic distance matrix calculated based on the similarity of morphotypes of L. dahurica and L. cajanderi populations were consistent with the results of their differentiation based on relative deformations of scales obtained using canonical discriminant analysis (See Fig. 4 and Fig. 7). In spite of the differences in the shape of the cone scales between the North-Yeniseisk and the Trans-Baikal populations of L. dahurica, they both differed from L. cajanderi populations. Thus, phenotypic analysis confirmed differentiation of these two larch species. Despite the similarities between a number of morphotypes, the Yakut L. cajanderi populations were differentiated from L. dahurica populations. Significant differences were noted between intraspecific groups: between L. cajanderi populations from Okhotsk-Kolyma Upland and Yakutia and between L. dahurica populations from Evenkia and the Trans-Baikal region (See Fig. 4). The similarities between species and intraspecific differences may be attributed to the ongoing processes of hybridization and species formation in the region where the ranges of the larches overlap with the ranges of L. czekanowskii Szafer and L. dahuricax L. cajanderi hybrids. Geometric morphometrics can be used as an effective tool for analyzing differentiation of L. dahurica and L. cajanderi in the shape of their cone scales. The paper contains 7 Figures, 1 Table and 38 References.

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Держатели документа:
Russian Acad Sci, Lab Plant Ecol, Kamchatka Branch Pacific Geog Inst, Far Eastern Branch, 19-A Rybakov Ave, Petropavlovsk Kamchatski 683024, Russia.
Russian Acad Sci, Lab Forest Genet & Breeding, VN Sukachev Inst Forest, Siberian Branch, 50-28 Academgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Lab Biogeochem Ecosyst, 79 Svobodny Ave, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Inst Biol Problems North, Lab Bot, Far Eastern Branch, 18 Portovaya Str, Magadan 685000, Russia.

Доп.точки доступа:
Vetrova, Valentina P.; Barchenkov, Alexey P.; Sinelnikova, Nadezhda, V

/ I. N. Tretyakova, M. E. Park, A. P. Pakhomova [и др.] // Vestn. Tomsk. Gos. Univ. Biol. - 2021. - Is. 54. - С. 6-20, DOI 10.17223/19988591/54/1. - Cited References:0. - The reported study was carried out within the framework of the basic project IL SB RAS-2021-2025 No. 0356-2021-0009 and partially financed by the Russian Foundation for Basic Research, Government of Krasnoyarsk Krai, Krasnoyarsk Region Science and Technology Support Fund (Project No. 19-44-240009). . - ISSN 1998-8591. - ISSN 2311-2077РУБ Biology + Ecology

Аннотация: The biotechnology of somatic embryogenesis in in vitro culture is the most promising direction in the reproduction of conifers. The use of this technology makes it possible not only to massively propagate the best genotypes of trees, but also serves a model for studying the structural, physiological and molecular and genetic mechanisms of both somatic and zygotic embryogenesis in conifers. The main aim of this research was to obtain embryogenic cultures (ECs) producing somatic embryos and embryonic suspension mass (ESM) of Picea obovata. The studies were carried out in 2014-2019 on 30 Siberian spruce trees growing in the vicinity of the city of Krasnoyarsk. To detect genotypes competent for somatic embryogenesis, new donor trees were selected every year for the experiment. 3-10 cones were collected from each tree at different stages of embryo development: globular embryo (the first decade of July), the initiation stage cotyledons (second decade of July), the stage of developed cotyledons (third decade of July) and mature embryos (August). Sterilized explants (zygotic embryos at different stages of development) were introduced into in vitro culture on basic media DCR (Gupta PK and Durzan DJ, 1985), 1/2LN (Litvay JD et al., 1985), MS (Murashige T and Skoog F, 1962) and AI (Tretyakova IN, 2012). All media were supplemented with myo-inositol - 100 mg/L, casein hydrolyzate - 500-1000 mg/L, L-glutamine - 500 mg/L, sucrose - 30 g/L and agar - 7 g/L. Ascorbic acid at a concentration of 400 mg/L was used as an antioxidant. The level of growth regulators was: 2,4-dichlorophenoxyacetic acid (2,4-D) - 2 mg/L and N-6-benzoaminopurine (BAP) - 1 mg/L. For the proliferation of the ESM, DCR and AI basic media containing 2,4-D (2 mg/L), BAP (0.5 mg/L) and sucrose (20 g/L) were used. The pH was adjusted to pH = 5.8. All culture medium and components were sterilized depending on their termolabile properties. Under aseptic conditions, embryos were removed from megagametophytes and inoculated into nutrient media, 10 explants per flask in 25 replicates. The cultures were incubated in the dark at 24 +/- 1 degrees C. Subcultivation to fresh nutrient medium was carried out every 14 days. To control the quality of cell lines (CL) during subculturing, we performed cytological analyzes using temporary preparations (3-5 preparations for each CL). We evaluated the quality of the embryogenicity of the cultures by the presence of even single structures with pronounced polarity - a globular embryo with a suspensor. The results of the study showed that the induction of callus cultures of Siberian spruce is influenced by such factors as the development stage of the explant, the nutrient medium and the genotype of the donor tree. The introduction of P. obovata immature zygotic embryos into in vitro culture at the stage of the globular embryo, both with megagametophytes and extracted from them, turned out to be ineffective. The induction of callus cultures in Siberian spruce was significantly reduced when mature zygotic embryos were introduced into the culture in vitro. The highest response of explants of Siberian spruce was at the stage of developed cotyledons (See Table 1). In the DCR medium, 90% of explants formed callus (See Table 2). The mineral composition of the media did not significantly affect the induction of callus formation in Siberian spruce. The exception was the MS medium, in which callus cultures were formed only in 41% of explants (See Table 2). The growth of callus cultures was most active in the DCR medium. After 6 months of cultivation, 15-32% of calli remained viable (See Table 2). Cytological analysis of callus cultures showed that they include cells of different types (See Fig. 1 and 2). The first type of cells consisted of elongated cells reaching a length of 10 +/- 3 gm, others consisted of isodiametric cells with a diameter of 60 +/- 3.5 gm. The somatic embryo globule and embryonic tubes were formed from elongated cells. Isodiametric cells were actively dividing and forming callus. Only 3 cell lines (out of 300 cell lines) belonging to two donor trees had an active ability to proliferate. Globular somatic embryos were actively forming in these cell lines (See Fig. 3). An actively proliferating ESM was formed. Thus, we carried out a comprehensive assessment of the factors influencing the induction of somatic embryogenesis in Siberian spruce. The results obtained indicate that for the successful formation of somatic embryos, the determining factor is not only the choice of donor plants, but also the development stage of the explant. We found that the best stage in the development of zygotic embryos when introduced into in vitro culture of Siberian spruce is the stage of immature embryos with formed cotyledons, while the DCR, 1/2LN and AI nutrient medium supplemented with growth regulators (2.4-D and BAP) is optimal.

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Держатели документа:
Russian Acad Sci, VN Sukachev Inst Forest, Siberian Branch, Lab Forest Genet & Breeding, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Siberian Fed Univ, Dept Aquat & Terr Ecosyst, 79 Svobodny Ave, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Tretyakova, Iraida N.; Park, Maria E.; Pakhomova, Angelica P.; Sheveleva, Irina S.; Muratova, Elena N.; Russian Foundation for Basic Research, Government of Krasnoyarsk Krai [IL SB RAS-2021-2025, 0356-2021-0009]; Krasnoyarsk Region Science and Technology Support Fund [19-44-240009]