Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / G. G. Polyakova [et al.] // Russ. J. Plant Physiol. - 2008. - Vol. 55, Is. 4. - P496-502, DOI 10.1134/S1021443708040109. - Cited References: 25 . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: The elicitor activity of compounds extracted from the mycelia of six species of phytopathogenic fungi was assessed from the sizes of necrotic lesions on the external surface of the living trunk phloem of five coniferous species inhabiting Siberia: Siberian larch (Larix sibirica L.), Scotch pine (Pinus sylvestris L.), Siberian spruce (Picea obovata Ledeb.), Siberian fir (Abies sibirica L.), and cedar pine (Pinus sibirica (Rupr.) Mayr.). The compounds for inoculation were extracted from the mycelium of ascomycetes imperfect, and basidium fungi; the living mycelia of these fungi were also used. The fungal extract or mycelium was placed into the hollows 7 mm in diameter in the trunk bark. Infection triggered the formation of hypersensitivity necrotic lesions in the inner bark exceeding in size those appeared after control wounding of four tree species (larch, pine, spruce, and cedar); fir was an exclusion. In experiments with tree trunks and conifer calluses, a dependence of immune response parameters (the sizes of necrotic lesions and the content of lignin and bound proanthocyanidins) on the quantity of the fungal preparation was elucidated. The largest necrotic lesions appeared after injection of 500 mu g of the fungal preparation into the hollow in the trunk, and its higher quantities did not increase the indices measured. The size of the necrotic lesion on the trunk bark is supposed to be used as a promising index characterizing the level of tree immunity and tolerance under various ecological conditions.

Полный текст,
WOS,
Scopus

Держатели документа:
[Polyakova, G. G.
Pashenova, N. V.
Polyakov, V. I.
Zrazhevskaya, G. K.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Pashenova, N.V.; Polyakov, V.I.; Zrazhevskaya, G.K.

[Text] / I. V. Shein [et al.] // Russ. J. Plant Physiol. - 2003. - Vol. 50, Is. 5. - P634-639, DOI 10.1023/A:1025688023862. - Cited References: 27 . - 6. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Pine (Pinus sylvestris L.) callus culture was treated with the mycelium extracts from six Fusarium strains. Previously, pine seedlings were infected with a spore suspension in order to test the pathogenicity of the used strains. Callus culture infection resulted in a decrease in the free proanthocyanidin (PA) and an increase in bound PA content. After treating the calli with all strains except F. oxysporum var. orthoceras, the lignin content became lower than the control one. The most considerable changes involved the p-hydroxybenzoic acid (HBA) content, and its greatest change was observed after treating the calli with F. nivale, when the HBA concentration (1229 mug/g, dry wt) exceeded fourfold the control one. There was a positive correlation (R = 0.81) between the HBA content in the callus culture cells treated with a fungal extract and the virulence of Fusarium strains. At the same time, there was an inverse correlation (R = -0.80) between the lignin content in a callus culture and the fungal virulence; the latter did not affect the contents of both free and bound PA.

Полный текст,
WOS,
Scopus

Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia
Ctr Forest Def Krasnoyarsk Krai, Krasnoyarsk, Russia

Доп.точки доступа:
Shein, I.V.; Andreeva, O.N.; Polyakova, G.G.; Zrazhevskaya, G.K.

[Text] / I. V. Shein [et al.] // Russ. J. Plant Physiol. - 2001. - Vol. 48, Is. 2. - P216-221, DOI 10.1023/A:1009056201926. - Cited References: 23 . - 6. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Callus cultures of Siberian larch (Lar ix sibirica Ledeb.) and Siberian spruce (Picea obovata Ledeb.) were used to demonstrate the elicitor activity of two ophiostomatoid fungal species, Ceratocystis laricicola and Ceratocystis polonica, as the pioneer settlers on larch and spruce, respectively. The extract from C. laricicola mycelium stimulated the accumulation of lignin in larch cells by 37% and that of hound proanthocyanidins by 25%. In spruce callus cultures, C. laricicola and C. polonica increased the bound PA content by 25 and 46%, respectively. In the callus cultures of larch and spruce, the addition of extract of C. laricicola increased the concentration of p-hydroxybenzoic acid 13-fold and nearly 4-fold, respectively. The metabolic characteristics of accumulation of phenolic compounds in conifer cells are discussed.

Полный текст,
WOS,
Scopus

Держатели документа:
Russian Acad Sci, Sukachev Forest Inst, Siberian Div, Akademgorodok 660036, Krasnoyarsk, Russia

Доп.точки доступа:
Shein, I.V.; Polyakova, G.G.; Zrazhevskaya, G.K.; Pashenova, N.V.; Vetrova, V.P.