Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / I. N. Tretyakova, N. V. Novoselova, Y. A. Cherepovskii // Russ. J. Plant Physiol. - 2004. - Vol. 51, Is. 1. - P120-126, DOI 10.1023/B:RUPP.0000011312.64979.0d. - Cited References: 12 . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Morphological and cytoembryological studies of unique forms of Siberian pine (Pinus sibirica Du Tour) with the annual cycle of ovulate cone development were carried out. The morphogenesis of female shoots in these anomalous trees with the annual cycle of development was shown to provide for an accelerated development and very rapid growth of megastrobili and accelerated formation of reproductive structures. Cytoembryological studies of ovules in these trees showed significant shortening of the free-nuclear stage of gametophyte development (up to 3 weeks instead of one year) and ultra-early formation of archegonia and egg cells. However, no fertilization of egg cells and the development of embryos occur in the anomalous forms; the egg-cell nucleus divides in the haploid state. Developing seeds are formed without embryo. The author hypothesizes that the acceleration of embryo development in the anomalous pine trees is caused by enhanced hormonal, carbohydrate, and nitrogen metabolism in ovule tissues.

Полный текст,
WOS,
Scopus

Держатели документа:
Russian Acad Sci, Siberian Div, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Tretyakova, I.N.; Novoselova, N.V.; Cherepovskii, Y.A.

/ I. N. Tret’yakova, N. V. Lukina // Russ. J. Dev. Biol. - 2016. - Vol. 47, Is. 1. - P41-48, DOI 10.1134/S1062360416010069 . - ISSN 1062-3604
Аннотация: The study of the formation of embryonic structures in Pinus sibirica forms with a one-year reproductive cycle showed that the acceleration of the embryonic process manifested itself as a reduction of the coenocytic stage of the female gametophyte development (1.5 months instead of 1 year). The egg was not fertilized because of the asynchronous maturation of male and female gametophytes. Seeds without embryos were formed. We assumed that the acceleration of the reproductive process in Pinus sibirica was caused by a mutation in the female generative organs. © 2016, Pleiades Publishing, Inc.

Scopus,
WOS

Держатели документа:
Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50, bld. 28, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Tret’yakova, I. N.; Lukina, N. V.

/ I. N. Tretyakova, A. V. Lukina // Russ. J. Dev. Biol. - 2017. - Vol. 48, Is. 5. - P340-346, DOI 10.1134/S1062360417050083. - Cited References:19. - I thank D.SC. Med. Sci. Prof. S.N. Goroshkevich for providing samples of hybrid cones of Pinus sibirica. The study was supported by the Russian Foundation for Basic Research, project no. 15-04-01427, and the Government of Krasnoyarskii krai, Krasnoyarsk Regional Fund for Support of Scientific and Scientific-Technical Activities, project no. 16-44-240509. . - ISSN 1062-3604. - ISSN 1608-3326РУБ Developmental Biology

Аннотация: Cytoembryological research of the ovules in experiments with interspecific hybridization of Pinus sibirica (pollination be the pollen of P. koraiensis, P. armandii, P. parviflora, P. strobus, P. hokkaidensis, P. wallichiana, P. monticola, and P. Nembra) revealed that the development of megagametophytes occurred in them by the usual scenario and resulted in the formation of mature archegonia. Pollen successfully germinated on the nucellus of ovules. However, disturbances were observed in the process of male gametophyte development, and pollen tubes on the nucellus were not visible by the period of archegonia maturation. Fertilization was usually absent. The development of embryonic channel is determined by egg cell maturity. The only exception was the variant of the controlled pollination of Pinus sibirica x P. Nembra, in which the embryo has been formed.

WOS,
Смотреть статью

Держатели документа:
Russian Acad Sci, Special Dept Forest Inst, Krasnoyarsk Sci Ctr, Fed Res Ctr,Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Tretyakova, I. N.; Lukina, A. V.; Russian Foundation for Basic Research [15-04-01427]; Government of Krasnoyarskii krai, Krasnoyarsk Regional Fund for Support of Scientific and Scientific-Technical Activities [16-44-240509]

/ I. N. Tret'yakova, M. E. Park // Russ. J. Dev. Biol. - 2018. - Vol. 49, Is. 4. - P222-233, DOI 10.1134/S1062360418040069. - Cited References:46. - The study was carried out with the financial support of the Russian Foundation for Basic Research, the Government of Krasnoyarsk krai, and the Krasnoyarsk Territory Fund for Support of Scientific and Technical Activity within the framework of scientific projects nos. 16-44-240509 and 16-44-243068. . - ISSN 1062-3604. - ISSN 1608-3326РУБ Developmental Biology

Аннотация: The initiation of somatic embryos and their propagation in the long-term proliferating embryonal suspensor mass of Larix sibirica were studied. It was found that the increase in the number of somatic embryos in the embryogenic culture occurred as a result of cleavage of the globules of the somatic embryo and the suspensor; it less often occurred as the result of budding of the suspensor and the separation of the embryonal tubes of the suspensor. In the case of long-term proliferating cell lines (more than 8 years), the rate of cleavage did not weaken. A conclusion on the identity of morphogenic processes underlying the development of zygotic and somatic embryos of conifers is made, which is confirmed by the concept of T.B. Batygina (1999) on the parallelism of their development in vivo and in vitro.

WOS,
Смотреть статью

Держатели документа:
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Div Krasnoyarsk Sci Ctr,Fed Res Ctr, Krasnoyarsk, Russia.

Доп.точки доступа:
Tret'yakova, I. N.; Park, M. E.; Russian Foundation for Basic Research; Government of Krasnoyarsk krai; Krasnoyarsk Territory Fund for Support of Scientific and Technical Activity [16-44-240509, 16-44-243068]

/ F. Gao, C. Peng, H. Wang [et al.] // Forests. - 2020. - Vol. 11, Is. 9. - Ст. 912, DOI 10.3390/F11090912 . - ISSN 1999-4907
Аннотация: Korean pine is the dominant species of Korean pine forests. It is an economically valuable species that yields oil, high-quality timber and nuts, and it offers great prospects for further development. Complete regenerated plants of Korean pine were obtained via somatic embryogenesis using megagametophytes as the explant. The seeds of 27 families of Korean pine were collected to induce embryogenic lines. We compared the effects of explant collection time, family and medium components (concentrations of sucrose, plant growth regulators and acid-hydrolyzed casein) on embryogenic lines induction. The effects of plant growth regulators and L-glutamine contents on the proliferation and maturation of embryogenic cell lines were studied, and the germinating ability of different cell lines was evaluated. The embryogenic lines induction percentage of Korean pine reached 33.33%. When 4.52 ?mol·L-1 2,4-D and 2.2 ?mol·L-1 6-BA were added to the medium of embryogenic lines proliferation, the ability of embryo maturation was the best (cell line 001#-100 was 135.71·g-1 fresh weight). Adding 1-1.5g L-1 L-glutamine to the proliferation medium can improve the ability of embryo maturation (cell line 001#-100 was 165.63·g-1 fresh weight). The germination percentage of the three cell lines tested was significant, and the highest was 66%. We report on successful regeneration and cryopreservation methods for somatic embryos of Korean pine. This technology could be used to propagate the excellent germplasm resources of Korean pine and to establish multi-varietal forestry. © 2020 by the authors.

Scopus

Держатели документа:
State Key Laboratory of Tree Genetics and Breeding, School of Forestry, Northeast Forestry University, Harbin, 150040, China
Laboratory of Forest Genetics and Breeding, Institution of the Russian Academy of Sciences V.N., Sukachev Institute of Forest Siberian Branch of RAS, Krasnoyarsk, 660036, Russian Federation
Department of Cell Biology and Institute of Plant Physiology K.A., Timiryazev Russian Academy of Sciences, Moscow, 127276, Russian Federation
Department of Plant Physiology, Biological Faculty, Lomonosov Moscow State University, Moscow, 119991, Russian Federation
State Forestry and Grassland Administration Engineering Technology Research Center of Korean Pine, Harbin, 150040, China

Доп.точки доступа:
Gao, F.; Peng, C.; Wang, H.; Nikolaevna, I.; Mikhaylovich, A.; Shen, H.; Yang, L.

/ И. Н. Третьякова // Лесоведение. - 2023. - № 6. - С. 608-616DOI 10.31857/S0024114823060104
   Перевод заглавия: BIOLOGICAL PROPERTIES OF THE SIBERIAN PINE’S SEEDS
Аннотация: Проведено изучение биологических свойств семян сосны сибирской (Pinus sibirica Du Tour). В работе использовали четыре фракции семян, отличающиеся по степени крупности: фракцию крупных семян (длина 11–14 мм), фракцию средних семян (длина 9–10 мм), фракцию мелких семян (длина 7–8 мм) и фракцию очень мелких семян (длина <6 мм). Изучение качества семян по данным рентгенографии и гистохимического анализа показало, что по размеру семян можно судить об их дефектности. Фракции мелких и очень мелких семян сосны сибирской оказались полностью стерильными, большая часть семян крупной и средней фракции – жизнеспособными. Цитоэмбриологические исследования показали, что у деревьев, формирующих пустые и недоразвитые семена (фракции средних и крупных семян), наблюдаются нарушения в развитии эмбриологических структур. Эти нарушения проходят на стадии гаметофитогенеза и развития архегониев. У деревьев с крупными семенами гаметогенез идет без отклонений, в семяпочках формируется четыре архегония, и образуются полиэмбриональные семена (до 16 эмбрионов в зародышевом канале). У деревьев сосны сибирской с однолетним репродуктивным циклом размер семян варьирует от очень мелких до крупных. У этих деревьев развитие женского гаметофита завершается и образуются архегонии. Однако в семяпочках деревьев с однолетним репродуктивным циклом оплодотворение яйцеклеток не происходит, и образуются семена без зародыша. За период стратификации (четыре месяца) у большинства семян средней и крупной фракции внутрисеменной рост зародыша полностью завершается. В культуре in vitro на среде MS c низкой концентрацией гормонов рост зародышевой оси осуществляется за семь дней культивирования. Зародыши успешно прорастают. Таким образом, на основании опытов по культивированию зародышей сосны сибирской можно сделать вывод о наличии у семян данного вида органического (морфофизиологического) покоя. При создании оптимальных условий для прорастания (питательного субстрата, температуры 24 ± 1°C) семена сосны сибирской способны прорастать.
A study of the biological properties of the Siberian pine (Pinus sibirica Du Tour) seeds was carried out. Four seed categories were used in the work, differing in size: large (11–14 mm in length), medium (9–10 mm), small (7–8 mm) and very small seeds (<6 mm). The seeds quality study using the X-ray and the histochemical analyses showed that the size of the seeds can indicate their defectiveness. Fractions of small and very small seeds of Siberian pine turned out to be completely sterile, most of the seeds of large and medium fractions were viable. Cytoembryological studies have shown that in trees that form empty and underdeveloped seeds (fractions of medium and large seeds), disturbances in the embryological structures development can be observed. These disturbances take place at the stage of gametophytogenesis and the archegonia development. In trees with large seeds, gametogenesis proceeds without deviations, four archegonia are formed in the ovules, and polyembryonic seeds are formed (up to 16 embryos in the germinal canal). In Siberian pine trees with a one-year reproductive cycle, the seed size varies from very small to large. In these trees, the development of the female gametophyte is completed and archegonia are formed. However, in the ovules of trees with a one-year reproductive cycle, fertilization of eggs does not occur and seeds without an embryo are formed. During the period of stratification (four months), in most seeds of the medium and large fractions the intraseed, growth of the embryo was completely completed. In in vitro culture on MS medium with a low concentration of hormones, the growth of the germinal axis was carried out in seven days of cultivation. The embryos germinated successfully. Thus, based on experiments on the Siberian pine embryos cultivation, it can be concluded that the seeds of this species can enter organic (morphophysiological) dormancy. When creating optimal conditions for germination (nutrient substrat, temperature 24 ± 1°C), Siberian pine seeds are able to germinate.

РИНЦ

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50/28

Доп.точки доступа:
Tret'yakova, Iraida Nikolayevna