Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ N. V. Oreshkova, M. M. Belokon, S. . Jamiyansuren // Russ. J. Genet. - 2013. - Vol. 49, Is. 2. - P178-186, DOI 10.1134/S1022795412120095. - Cited References: 57. - This work was supported by the Russian Foundation for Basic Research, project nos. 10-04-00786-a, 11-04-00478-a, and 11-04-92112-YaF_a. . - 9. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: The genetic diversity of SSR markers was studied in six populations of Siberian larch, two popu-lations of Gmelin larch, and four populations of Cajander larch. Seven pairs of nuclear microsatellite loci were used for this analysis. 103 allelic variants were detected in 365 individuals of three species of larch. According to the AMOVA results, the variability proportion that characterizes the differences between three Larix species was twice as higher (15%) than the proportion that accounts for among-population differences within the species (6%). The differentiation of the populations of three species of larch based on SSR markers exceeded 12% (F (ST) = 0.121). A significant correlation of the genetic distances with the geographic distances between populations was found (r = 0.835, P = 0.01).

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Держатели документа:
[Oreshkova, N. V.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia
[Belokon, M. M.] Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 119991, Russia
[Jamiyansuren, S.] Mongolian Acad Sci, Inst Bot, Ulaanbaatar 210523, Mongol Peo Rep

Доп.точки доступа:
Oreshkova, N.V.; Belokon, M.M.; Jamiyansuren, S...

/ N. A. Tikhonova, M. A. Polezhaeva, E. A. Pimenova // Russ. J. Genet. - 2012. - Vol. 48, Is. 10. - P985-992, DOI 10.1134/S1022795412100110. - Cited References: 41 . - 8. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: Using AFLP analysis, the genetic variation in four population samples of Rhododendron ledebourii, three such samples of Rh. dauricum, two samples of Rh. sichotense, and one sample of Rh. mucronulatum was examined. Application of three selective primer pairs provided genotyping of 87 accessions from the four species at 213 loci. An analysis of molecular variance revealed a high level of differences between all four species (F (ST) = 0.172). Populations within the species were also differentiated: for Rh. dauricum F (ST) = 0.245; Rh. ledebourii F (ST) = 0.264; Rh. sichotense F (ST) = 0.034. The highest Nei distance was observed for Rh. mucronulatum with Rh. dauricum and Rh. ledebourii (D = 0.057 and 0.069, respectively). Similarity was observed between the species pairs of Rh. ledebourii-Rh. dauricum (0.032) and Rh. sichotense-Rh. mucronulatum (0.034). The Far Eastern species Rh. sichotense and Rh. mucronulatum were characterized by an elevated level of intrapopulation variation. The data from the AFLP-based analysis of differentiation and variation that were thus obtained were compared with the literature data on the morphological criteria that differentiate the species of interest and those regarding the history of the formation of their ranges. The independence of all the species we examined was confirmed.

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Держатели документа:
[Tikhonova, N. A.] Russian Acad Sci, Sukachev Forest Inst, Krasnoyarsk 660036, Russia
[Polezhaeva, M. A.] Russian Acad Sci, Inst Plant & Anim Ecol, Ekaterinburg 620144, Russia
[Pimenova, E. A.] Abramov Sikhote Alin State Biosphere Reserve, Primorsky Krai 692150, Terney, Russia

Доп.точки доступа:
Tikhonova, N.A.; Polezhaeva, M.A.; Pimenova, E.A.

[Text] / A. N. Kravchenko, A. Y. Larionova, L. I. Milyutin // Russ. J. Genet. - 2008. - Vol. 44, Is. 1. - P35-43, DOI 10.1134/S1022795408010055. - Cited References: 42 . - 9. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: Based on the analysis of 22 loci controlling allozyme variation of MDH, SKDH, 6-PGD, IDH, PEPCA, GOT, FDH, LAP, PGI, PGM, SOD, and GDH, the data on within-and among-population variation for nine cenopopulations of Siberian spruce (Picea obovata Ledeb.) located along the Yenisei mieridian, from 65 degrees 50' NL to 52 degrees 14' NL, were obtained. It was demonstrated that 86.36% of the loci, tested in the species analyzed, were polymorphic. The mean number of alleles per locus was 2.91, and the observed and expected heterozygosity constituted 0.161 and 0.168, respectively. More than 97% of total variation occurred within the populations, while the proportion of the among-population variation constituted only 2.3% (F-st =0.0230). Genetic distance (D-N) between the populations examined varied from 0.0019 to 0.0115, averaging at 0.0051. It was shown that in the part of the Siberian spruce range examined there was no close association between the level of the genetic differentiation of the populations and the geographic distance between them. It seems likely, that this finding can be associated with the fact that Siberian spruce, growing on this territory, is the intrazonal species, i.e., it is not attached to a certain forest zone, and its distribution is mostly determined by local ecological conditions.

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Держатели документа:
[Kravchenko, A. N.
Larionova, A. Ya.
Milyutin, L. I.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Kravchenko, A.N.; Larionova, A.Y.; Milyutin, L.I.

[Text] / A. Y. Larionova, N. V. Yakhneva, A. P. Abaimov // Russ. J. Genet. - 2004. - Vol. 40, Is. 10. - P1127-1133, DOI 10.1023/B:RUGE.0000044756.55722.d8. - Cited References: 32 . - 7. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: Within- and among-population diversity of Gmelin larch Larix gmelinii (Rupr.) Rupr. from Evenkia was inferred from data on 17 genes determining allozyme diversity of ten enzymes. More than 50% of the genes proved to be polymorphic. On average, each tree was heterozygous at 9.2% genes. Heterozygosity expected from the Hardy-Weinberg proportions was higher, 12.5%. A deficit of heterozygous genotypes was observed in all populations under study and attributed to inbreeding. With Wright's F statistics, average individual inbreeding was estimated at 26.6% relative to the population (F-IS) and at 27.8% relative to the species (F-IT). The greatest deficit of heterozygosity was observed for the youngest population II. Within- population variation accounted for more than 98% of the total variation, while the contribution of among-population variation was 1.66%. Genetic distance between populations varied from 0.0025 to 0.0042, averaging 0.0035.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Larionova, A.Y.; Yakhneva, N.V.; Abaimov, A.P.

[Text] / A. Y. Larionova // Russ. J. Genet. - 2002. - Vol. 38, Is. 12. - P1391-1396, DOI 10.1023/A:1021639806123. - Cited References: 34 . - 6. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: The main parameters of genetic variability have been determined in an isolated natural Scotch pine population from Chita oblast (Tsasuchei Forest) by analysis of 19 genes coding for nine enzymes: GDH, IDH, LAP, PGM, AAT, ADH, MDH, 6-PGD, and DIA. Polymorphic genes constituted 63.2% of all structural genes studied in the population at the 99% polymorphism criterion. The mean number of alleles per locus was 1.63. The observed and expected heterozygosities were 0.237 and 0.251, respectively. These estimates are close to the corresponding mean values for Scotch pine according to the data on 18 or more structural genes.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Larionova, A.Y.

[Text] / N. V. Pashenova, V. P. Vetrova, G. G. Polyakova ; ed.: F Lieutier, WJ Mattson, WJ Mattso // PHYSIOLOGY AND GENETICS OF TREE-PHYTOPHAGE INTERACTIONS - INTERNATIONAL SYMPOSIUM. Ser. COLLOQUES DE L INRA : INST NATL RECHERCHE AGRONOMIQUE, 1999. - International Symposium on Physiology and Genetics of Tree-Phytophage Interactions (AUG 31-SEP 05, 1997, GUJAN, FRANCE), Is. 90. - P261-271. - Cited References: 19 . - 11. - ISBN 0293-1915. - ISBN 2-7380-0883-6РУБ Plant Sciences + Forestry

Аннотация: Effect of tannin and non-volatile components of lesion resin on the growth of blue-stain fungi - Ceratocystis laricicola, C. polonica, Ophiostoma minus, Leptographium sp. and Ophiostoma sp. - the primary invaders of conifers in Siberia (Russia), has been studied. The fungi under study exhibited tolerance to plant's defensive substances in bioassays. The cultures of Leptographium sp., C. polonica, and Ophiostoma sp. proved to be the most tolerant to tannin. In the range between 0.05 and 0.15% tannin concentration their growth tended to stabilize. Less tolerant to tannin were C. laricicola and O. minus. With an increase in tannin concentration their biomass gradually decreased. The fungi more tolerant to tannin regulated the medium pH about 5.0 and higher, white the species which were less tolerant preferred lower pH values. Lesion resins of conifers differed in their inhibitory effect on fungal growth. Fir resin had the greatest inhibitory effect of all the conifer resins tested, causing reduction to 50-60% in fungal growth. When fungi were grown on the resin of any other conifer, the reduction in the linear growth rate did not exceed 20 to 30%. The assumption that fungi are more tolerant to the resin of a host plant than to that of a non-host plant has not been confirmed.

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Держатели документа:
Russian Acad Sci, Inst Forest, Microbiol Lab, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Pashenova, N.V.; Vetrova, V.P.; Polyakova, G.G.; Lieutier, F \ed.\; Mattson, WJ \ed.\; Mattso, WJ \ed.\

[Text] / V. P. Vetrova, V. V. Stasova, N. V. Pashenova ; ed.: F Lieutier, WJ Mattson, WJ Mattso // PHYSIOLOGY AND GENETICS OF TREE-PHYTOPHAGE INTERACTIONS - INTERNATIONAL SYMPOSIUM. Ser. COLLOQUES DE L INRA : INST NATL RECHERCHE AGRONOMIQUE, 1999. - International Symposium on Physiology and Genetics of Tree-Phytophage Interactions (AUG 31-SEP 05, 1997, GUJAN, FRANCE), Is. 90. - P287-297. - Cited References: 13 . - 11. - ISBN 0293-1915. - ISBN 2-7380-0883-6РУБ Plant Sciences + Forestry

Аннотация: Effect of defoliation on resistance response of Siberian fir, Abies sibirica Ledeb., to fungal inoculation was investigated within three even-aged fir stands damaged by Siberian moth, Dendrolimus superans sibiricus Tschetw., and located in the Krasnoyarsk Territory (Central Siberia). Histological changes in tissues, accompanying the resistance response were examined in 30 fir trees (90-130 years old) of different defoliation degree (50, 75, 100 %) six weeks after single inoculation with Leptographium sp, vectored by Monochamus urussovi Fisch. (Coleoptera: Cerambycidae). It was recorded that the rate and intensity of defence response of phloem and xylem tissues to inoculation as well as percent of trees infested by the insect-vector differed significantly in dependence of defoliation degree. Trees of 50 percent defoliation significantly differed from trees of more severe defoliation in all parameters of defence response and percentage of colonisation by the beetle. The trees defoliated to 75 and 100% did not differ significantly in defence response activity and the number of the trees attacked by M. urussovi Strong difference in resistance to inoculation and colonisation by the beetle was recorded between trees defoliated in the current year and in the previous year. All infested by M, urussovi trees were characterised by the epidemic level of frequency of blue-stain fungi associated with this insect. The tendency was recorded to a decreased frequency of the pathogenic Ophiostoma sp. and a more frequent occurrence of the saprophyte O. curvicollis in trees defoliated in previous year which lost ability to defend against wounding and fungal infection.

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Держатели документа:
Russian Acad Sci, Lab Forest Zool, Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Vetrova, V.P.; Stasova, V.V.; Pashenova, N.V.; Lieutier, F \ed.\; Mattson, WJ \ed.\; Mattso, WJ \ed.\

/ N. V. Ekimova, E. N. Muratova, P. P. Silkin // Russian Journal of Genetics: Applied Research. - 2012. - Vol. 2, Is. 2. - P105-109, DOI 10.1134/S2079059712020037 . - ISSN 2079-0597
Аннотация: Chromosome numbers for some species of Central Asia steppe shrubs are given: Rhamnus erythroxylon Pall. (Rhamnaceae), Caragana buriatica Peschk. (Fabaceae), Amygdalus pedunculata Pall., Armeniaca sibirica (L.) Lam. (Rosaceae), Atraphaxis pungens (Bieb.) Jaub. et Spach, and A. frutescens (L.) C. Koch (Polygonaceae). Chromosome numbers of some species were determined for the first time. A comparative analysis of adaptive properties of polyploidy and diploid species has been conducted. It was established that natural polyploids are more adaptive to existence in extreme conditions. They possess a high potential for survival and are characterized by a higher level of intraspecific polymorphism, abundant flowering and fruitification, ability to propagate both via generative and vegetative means, and high seed germination. В© 2012 Pleiades Publishing, Ltd.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
V.N. Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Ekimova, N.V.; Muratova, E.N.; Silkin, P.P.

/ E. N. Muratova, O. S. Bladimirova, T. V. Karpiuk // Tsitologiia. - 2004. - Vol. 46, Is. 1. - С. 79-86 . - ISSN 0041-3771
Аннотация: Results of karyological study of Picea ajanensis (Lindl. et Gord.) Fisch. ex Carr examined from 13 provenances are presented. In addition to the cytotypes with typical chromosome number (2n = 24), P. ajanensis displays cytotypes with one or two B-chromosomes (2n = 24 + 1 - 2B). Among A-chromosomes, there are 8 pairs of long metacentrics and 4 pairs of shorter meta- or submetacentrics. Among B-chromosomes there are two types of chromosomes: metacentric (B1) and submetacentric (B2) ones. There are many nucleolar chromosomes. Several chromosomes have secondary constrictions. Patterns of B-chromosome distribution within P. ajanensis are have been discussed.

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Держатели документа:
V. N. Sukachev Institute of Forest, Siberian Branch of the RAS, Krasnoyarsk.

Доп.точки доступа:
Muratova, E.N.; Bladimirova, O.S.; Karpiuk, T.V.

/ D. L. Grodnitskii // Zhurnal obshchei biologii. - 2000. - Vol. 61, Is. 4. - С. 371-380 . - ISSN 0044-4596
Аннотация: Neo-Darwinism is a result of synthesis of Darwinian concept of natural selection with Weismannian concept of germ plasma. The concept of germ plasma is based on a hypothesis that phenotypic traits are completely determined by genes. Hence, neo-Darwinism describes evolution as a process of alternation of gene frequencies under the effect of natural selection. This is an inadequate approach to the study of evolution. In the course of evolution, genes change their functions, whereas phenotypic characters change their corresponding genes. As a result, every step of evolutionary transformation changes the structure of phenotype-to-genotype correspondence. Therefore, phenotypic evolution cannot be described in genetic terms, the same as to human languages cannot be translated one into another whenever the meaning of words is constantly changing. Consequently, Weismannian germ-plasma concept adequately describes the relation of characters to genes only during stasis, but is inapplicable to evolution.

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Держатели документа:
Sukachev Institute of Forest Research, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russia.

Доп.точки доступа:
Grodnitskii, D.L.

/ A. I. Bolsunovskii [и др.] // Radiats Biol Radioecol. - 2007. - Vol. 47, Is. 1. - С. 63-73 . - ISSN 0869-8031
Аннотация: The study was devoted to investigation of the contents of radionuclides and of heavy metals and to evaluate the frequency of chromosomal aberrations in samples of Elodea canadensis, a submerged plant, collected in different parts of the Yenisei River. The samples were collected in the area subjected to radioactive impact of the Mining-and-Chemical Combine (MCC) at Zheleznogorsk and in the control area, upstream of the MCC. The investigations shown that elodea biomass in the area affected by MCC operation contained a long inventory of artificial radionuclides typical for the MCC discharges. The upstream of the MCC, in the control sampling area, the sediments and the elodea biomass contained only one artificial radionuclide--137Cs. Thus, the exposure doses to elodea shoots and roots upstream of the MCC are small (not more than 8 microGy/d) and the main contribution info the dose is made by natural radionuclides. At the MCC discharge site (the village of Atamanovo) and at the downstream of it, the total dose rate increases almost an order of magnitude, reaching its maximal values--72 microGy/d for elodea shoots and 58 microGy/d for its roots. Cytogenetic investigations of elodea roots shown that at the MCC discharge site (the village of Atamanovo) and at downstream of it the occurrence of chromosomal aberrations in ana-telophase and in metaphase cells of elodea was considerably higher than in the control area. It is highly probable that this simultaneous dramatic increase in the total exposure rate and the occurrence of chromosomal aberrations in elodea is associated with the radiation factor. It is suggested that elodea is affected not only by the radiation factor but also by the chemical factor--toxicity of heavy metals.

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Доп.точки доступа:
Bolsunovskii, A.I.; Muratova, E.N.; Sukovatyi, A.G.; Pimenov, A.V.; Sanzharaeva, E.A.; Zotina, T.A.; Sedel'nikova, T.S.; Pan'kov, E.V.; Kornilova, M.G.

[Text] / A. N. Tashev, T. S. Sedel'nikova, A. V. Pimenov // Cytol. Genet. - 2014. - Vol. 48, Is. 3. - P160-165, DOI 10.3103/S0095452714030116. - Cited References: 41 . - ISSN 0095-4527. - ISSN 1934-9440РУБ Genetics & Heredity

Аннотация: Investigations on B chromosomes found for the first time for Picea abies (L.) H. Karst. have been conducted. Seeds of Picea abies from two populations of Western Rhodopes (Bulgaria) located at the southern border of species range, and protected according to Bern Convention and EC Habitat Directive were collected for this study. Mixoploidy was detected in some germinating seeds of Picea abies. It was found that metaphase cells of germinating seeds contain 0-4 B chromosomes of both metacentric and submetacentric types. The variability of B chromosomes number and their occurrence was observed. Along with B chromosomes, some chromosome aberrations such as fragments and ring chromosomes were revealed in metaphase cells of Picea abies from studied populations. The possible adaptive role of B chromosomes presence for Picea spp. is discussed.

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[Tashev, A. N.] Univ Forestry, Sofia 1756, Bulgaria
[Sedel'nikova, T. S.
Pimenov, A. V.] Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia
ИЛ СО РАН

Доп.точки доступа:
Tashev, A.N.; Sedel'nikova, T.S.; Pimenov, A.V.

[Text] / V. L. Semerikov [et al.] // Russ. J. Genet. - 2014. - Vol. 50, Is. 6. - P577-585, DOI 10.1134/S1022795414040127. - Cited References: 38. - This work was supported by the Russian Foundation for Basic Researches, project nos. 11-04-92226-Mong_a and 12-04-00062-a, and by the Program of the Ural Branch of the Russian Academy of Sciences, project no. 12-I-4-2064. The authors thank the director of the Institute for Botany, Academy of Science of Mongolia, Academician Ch. Dzhugarzhav, and the director of the branch of FBI "Roslesoashchita" Centre of Forest Protection, Altai region, A.Ya. Bondarev, for help with material collection. . - ISSN 1022-7954. - ISSN 1608-3369РУБ Genetics & Heredity

Аннотация: The variability of four microsatellite loci of chloroplast DNA was studied in 38 populations of Pinus sylvestris in the European part of Russia, in Siberia, Kazakhstan, Transbaikalia and Mongolia. High variability was observed in all regions. In total, 152 haplotypes were identified. The level of population differentiation R (ST) was 2.1%. The differentiation of three geographical groups of populations (European, Siberian-Kazakhstan and Transbaikalian-Mongolian) was insignificant (R (CT) = 0.004). At the same time, some rare haplotypes were found to be specific for a certain geographical region. Distribution of the rare haplotypes, which differentiated European populations from Asian populations and Mongolian and Transbaikalian populations from the Siberian, showed the independence of the history of these regions. This corresponds more to the hypothesis that the modern area of Pinus sylvestris originated via settlement from many origins than to the hypothesized single center of the post-glacial recolonization. The distribution of the pairwise differences between the individual specimens corresponded to the model of sudden population growth. The assessments of the age of this event for Pinus sylvestris (4.5-4.7 million years), which were obtained on the basis of this model, significantly exceeded the age of the Quaternary. Therefore, the revealed population growth is hardly due to the changes in flora related with the glaciation, but rather mirrors the moment of the species formation.

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Держатели документа:
[Semerikov, V. L.
Semerikova, S. A.
Dymshakova, O. S.] Russian Acad Sci, Inst Plant & Anim Ecol, Ural Branch, Ekaterinburg 620144, Russia
[Zatsepina, K. G.
Tarakanov, V. V.
Tikhonova, I. V.
Ekart, A. K.] Russian Acad Sci, Sukachev Forest Inst, Siberian Branch, Krasnoyarsk 660036, Russia
[Vidyakin, A. I.] Russian Acad Sci, Inst Biol, Ural Branch, Komi Sci Ctr, Kirov 610035, Russia
[Jamiyansuren, S.] Acad Sci Mongolia, Inst Bot, Ulaanbaatar 210361, Mongol Peo Rep
[Rogovtsev, R. V.] Ctr Forest Protect Novosibirsk Reg, Novosibirsk 630015, Russia
[Kalchenko, L. I.] Ctr Forest Protect Altai Reg, Barnaul 656056, Russia
ИЛ СО РАН

Доп.точки доступа:
Semerikov, V.L.; Semerikova, S.A.; Dymshakova, O.S.; Zatsepina, K.G.; Tarakanov, V.V.; Tikhonova, I.V.; Ekart, A.K.; Vidyakin, A.I.; Jamiyansuren, S...; Rogovtsev, R.V.; Kalchenko, L.I.; Russian Foundation for Basic Researches [11-04-92226-Mong_a, 12-04-00062-a]; Program of the Ural Branch of the Russian Academy of Sciences [12-I-4-2064]

[Text] / N. V. Oreshkova [et al.] // Russ. J. Genet. - 2014. - Vol. 50, Is. 9. - P934-941, DOI 10.1134/S1022795414090105. - Cited References: 36. - The authors gratefully acknowledge the scientific director of the Center for Genomic Research of the Siberian State University (Krasnoyarsk), Senior Investigator at the Vavilov Institute of General Genetics, Russian Academy of Sciences (Moscow), Professor of Texas Agromechanical University (USA), and the University of Goettingen (Germany), Konstantin V. Krutovsky for assistance in interpreting the data and advice in writing this article. This work was supported by the Program of the Presidium of Russian Academy of Sciences no. 30 "Wildlife: Current Status and Problems of Development." . - ISSN 1022-7954. - ISSN 1608-3369РУБ Genetics & Heredity

Аннотация: We evaluated the population structure of the bog and dry land populations of the Siberian pine Pinus sibirica (P. sibrica) in Western Siberia using nuclear genome markers. Six pairs of nuclear microsatellite loci were used for this analysis. We detected 30 allelic variants in 120 individuals of four populations of P. sibirica. We established that the studied populations differ by genetic structure. The most essential differences were identified between the Siberian pine population from oligotrophic bog and the group of populations from dry land within eutrophic bogs and near settlements P. sibirica forest (F (ST) = 0.019; D (N) = 0.053). We estimated that diversification of the West Siberian populations of P. sibirica exceeded 2.4% (F (ST) = 0.024), based on an analysis of SSR markers.

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Держатели документа:
[Oreshkova, N. V.
Sedel'nikova, T. S.
Pimenov, A. V.
Efremov, S. P.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia
ИЛ СО РАН

Доп.точки доступа:
Oreshkova, N.V.; Sedel'nikova, T.S.; Pimenov, A.V.; Efremov, S.P.; Presidium of Russian Academy of Sciences [30]

[Text] / V. L. Semerikov [et al.] // Russ. J. Genet. - 2015. - Vol. 51, Is. 12. - P1199-1203, DOI 10.1134/S1022795415120108. - Cited References:20. - We thank Y.Y. Hhrunyk, A. I. Vidjakin, V.V. Tarakanov, E.V. Hantemirova, and I.V. Tikhonova for assistance with the pine material collection. The study was supported by Russian Foundation for Basic Research (grant 13-04-01028) and by Russian Federation Government (grant 14.Y26.31.0004). . - ISSN 1022-7954. - ISSN 1608-3369РУБ Genetics & Heredity

Аннотация: Fragments of genomic DNA of Scots pine (Pinus sylvestris L.) homologous to the mitochondrial DNA (mtDNA) contigs of Norway spruce (Picea abies (L.) Karst.) and loblolly pine (Pinus taeda L.) were resequenced in a sample of the Scots pine trees of European, Siberian, Mongolian, and Caucasian origin in order to develop mtDNA markers. Flanking non-coding regions of some mitochondrial genes were also investigated and resequenced. Five single nucleotide polymorphisms (SNPs) and a single minisatellite locus were identified. Caucasian samples differed from the rest by three SNPs. Two SNPs have been linked to an early described marker in the first intron of the nad7 gene, and all together revealed three haplotypes in European populations. No variable SNPs were found in the Siberian and Mongolian populations. The minisatellite locus contained 41 alleles across European, Siberian, and Mongolian populations, but, this locus demonstrated a weak population differentiation (F (ST) = 5.8), probably due to its high mutation rate.

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Держатели документа:
Russian Acad Sci, Ural Branch, Inst Plant & Anim Ecol, Ekaterinburg 620144, Russia.
Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Univ Gottingen, D-37077 Gottingen, Germany.
Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 119991, Russia.
Texas A&M Univ, College Stn, TX 77843 USA.

Доп.точки доступа:
Semerikov, V. L.; Putintseva, Yu. A.; Oreshkova, N. V.; Semerikova, S. A.; Krutovsky, K. V.; Russian Foundation for Basic Research [13-04-01028]; Russian Federation Government [14.Y26.31.0004]

/ E. A. Babushkina [et al.] // Dendrochronologia. - 2016. - Vol. 38. - P26-37, DOI 10.1016/j.dendro.2016.02.005 . - ISSN 1125-7865
Аннотация: The genetic mechanisms underlying the relationship of individual heterozygosity (IndHet) with heterosis and homeostasis are not fully understood. Such an understanding, however, would have enormous value as it could be used to identify trees better adapted to environmental stress. Dendrochronology data, in particular the individual average radial increment growth of wood measured as the average tree ring width (AvTRW) and the variance of tree ring width (VarTRW) were used as proxies for heterosis (growth rate measured as AvTRW) and homeostasis (stability of the radial growth of individual trees measured as VarTRW), respectively. These traits were then used to test the hypothesis that IndHet can be used to predict heterosis and homeostasis of individual trees. Wood core and needle samples were collected from 100 trees of Siberian larch (Larix sibirica Ledeb.) across two populations located in Eastern Siberia. DNA samples were obtained from the needles of each individual tree and genotyped for eight highly polymorphic microsatellite loci. Then mean IndHet calculated based on the genotypes of eight loci for each tree was correlated with the statistical characteristics of the measured radial growth (AvTRW and VarTRW) and the individual standardized chronologies. The analysis did not reveal significant relationships between the studied parameters. In order to account for the strong dependence of the radial growth on tree age the age curves were examined. An original approach was employed to sort trees into groups based on the distance between these age curves. No relationship was found between these groups and the groups formed based on heterozygosity. However, further work with more genetic markers and increased sample sizes is needed to test this novel approach for estimating heterosis and homeostasis. © 2016 Elsevier GmbH.

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Держатели документа:
Khakasia Technical Institute, Siberian Federal University, 27 Shchetinkina St., Abakan, Russian Federation
Siberian Federal University, Pr. Svobodniy 79, Krasnoyarsk, Russian Federation
V.N. Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50/28, Krasnoyarsk, Russian Federation
Georg-August-University of Gottingen, Busgenweg 2, Gottingen, Germany
N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, 3 Gubkina St., Moscow, Russian Federation
Texas AandM University, College Station, TX, United States
Institute of Geography, Russian Academy of Sciences, 29 Staromonetniy Pereulok, Moscow, Russian Federation

Доп.точки доступа:
Babushkina, E. A.; Vaganov, E. A.; Grachev, A. M.; Oreshkova, N. V.; Belokopytova, L. V.; Kostyakova, T. V.; Krutovsky, K. V.

/ A. K. Ekart [et al.] // Russ. J. Gen. - 2016. - Vol. 52, Is. 3. - P273-280, DOI 10.1134/S1022795416030054 . - ISSN 1022-7954
Аннотация: The variability of 21 allozyme and three microsatellite loci of chloroplast DNA (cpDNA) was studied in the populations of Siberian spruce (Picea obovata Ledeb.) from Irkutsk oblast, Magadan oblast, Buryatia, and Mongolia. It was demonstrated that the highest level of genetic diversity among the examined populations at both allozyme and microsatellite loci was observed in the Tulyushka population from Irkutsk oblast. The lowest level of genetic diversity was observed in marginal isolated populations of Bogd Uul and Magadan. In the relict spruce population from Olkhon Island, differing from the other populations in the lowest allelic diversity of both types of markers, no expected decline of expected heterozygosity and haplotype diversity was observed. In this population, the variability parameters mentioned were close to the population mean. The obtained intrapopulation and intraspecific variability parameters of allozyme and microsatellite loci of chloroplast DNA and the data on the population differentiation at these loci indicate that the given markers can be used for the analysis of the population structure of Siberian spruce. © 2016, Pleiades Publishing, Inc.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Plant and Animal Ecology, Ural Branch, Russian Academy of Sciences, Yekaterinburg, Russian Federation

Доп.точки доступа:
Ekart, A. K.; Semerikova, S. A.; Semerikov, V. L.; Larionova, A. Y.; Kravchenko, A. N.; Dymshakova, O. S.

/ V. P. Vetrova [et al.] // Russ. J. Genet. Appl. Res. - 2016. - Vol. 6, Is. 5. - P560-568, DOI 10.1134/S2079059716050142 . - ISSN 2079-0597
Аннотация: The structures of Picea ajanensis populations were compared based on the allozyme analysis of vegetative buds and the morphometric analysis of generative organs. Six cenopopulations of P. ajanensis were investigated in areas with various levels of volcanic impact in the Kamchatka Peninsula. The genetic structures of spruce populations and phenotypes were determined by the analysis of ten enzyme systems (PGM, GOT, HK, LAP, MDH, SKDH, IDH, GDH, PGI, and SOD). The phenotypic variability of spruce populations was estimated based on the composition of morphotypes that were identified by using the geometric morphometrics of cone-scale shapes. Pairwise comparison of samples of cones from 170 trees from six populations revealed 12 morphotypes differing in the shape of the cone scales. Comparative assessment of the variability and similarity of the populations was carried out based on the frequency of the occurrence of the phenotypes and the frequency of the alleles of the polymorphic loci. Correlations of the genetic and phenotypic distance matrices between different phenotypes were revealed. This observation was consistent with the genetic determination of the shape of the cone scales in spruce. Genetic differences between the morphotypes with regard to nine polymorphic loci (Got-2, Skdh-1, Idh-2, Pgm-2, Mdh-1, Mdh-3, Pgm-1, Pgi-2, and Hk) were not significant. Statistically significant differences between the spruce morphotypes were revealed for two loci: Pgm-2 and Mdh-1. Differences in the genetic diversity of spruce populations generally corresponded to differences in their phenotypic diversity. The high levels of genetic and phenotypic diversity characterized a stable population structure of spruce in the area of weak volcanic influence. Changes in the genetic structure and low levels of the phenotypic diversity of spruce were observed under catastrophic volcanic impact. © 2016, Pleiades Publishing, Ltd.

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Держатели документа:
Kamchatka Branch, Pacific Geographical Institute, Far East Branch, Russian Academy of Sciences, Petropavlovsk-Kamchatsky, Russian Federation
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vetrova, V. P.; Ekart, A. K.; Kravchenko, A. N.; Larionova, A. Y.

/ I. V. Tikhonova [et al.] // Russ. J. Gen. - 2016. - Vol. 52, Is. 9. - P926-938, DOI 10.1134/S1022795416090155 . - ISSN 1022-7954
Аннотация: We performed an analysis of linkage disequilibrium of alleles of 15 allozyme loci in 35 populations of Scots pine in the Siberian part of the range between 50° and 56° N and 79° and 107° E. We found a significant deviation in the distribution of genotypes of 1–9 pairs of loci in the investigated samples. We established correlations between frequencies of certain pairs of linked loci with ecological conditions and geographical coordinates of habitats of the pine populations. We present the results of comparative analysis of the differences in certain morphological features of trees with significantly predominant genotypes for pairs of linked loci in three isolated populations: in the south of Krasnoyarsk krai, Khakassia, and Tuva. © 2016, Pleiades Publishing, Inc.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Tikhonova, I. V.; Ekart, A. K.; Kravchenko, A. N.; Zatsepina, K. G.

/ A. N. Kravchenko, A. K. Ekart, A. Y. Larionova // Russ. J. Gen. - 2016. - Vol. 52, Is. 11. - P1142-1148, DOI 10.1134/S1022795416090088 . - ISSN 1022-7954
Аннотация: The results of the study of 21 populations of Siberian spruce (Picea obovata Ledeb.) from different parts of the species natural range by microsatellite (SSR) analysis of nuclear DNA are presented. Using nine loci developed for Picea abies (L.) Karst. and Picea glauca (Moench) Voss and detecting variation in Picea obovata, the parameters of intra- and interpopulation genetic diversity, as well as the degree of population differentiation, were determined. It was demonstrated that the population of Siberian spruce in the study was characterized by a relatively high average level of intrapopulation variability (Ho = 0.408; He = 0.423) and low interpopulation differentiation (Fst = 0.048, P = 0.001) at this class of DNA markers. The genetic distance between populations ranged from 0.009 to 0.167, averaging 0.039. The isolated Magadan population, located in the extreme Northeast of Russia at a considerable distance from the main species range and characterized by the lowest genetic diversity among the studied populations, was maximally differentiated from the rest of the spruce populations. In addition, the steppe Ubukun population from Buryatia and the population from the Bogd Khan Uul Biosphere Reserve, Mongolia, were considerably different in the genetic structure from most populations of Siberian spruce, although to a lesser extent than the Magadan population. These findings are consistent with the results of previous studies of this species carried out using allozyme and microsatellite loci of chloroplast DNA and point to the prospects of using nuclear microsatellites as DNA markers to analyze the population genetic structure of Siberian spruce. © 2016, Pleiades Publishing, Inc.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kravchenko, A. N.; Ekart, A. K.; Larionova, A. Y.