Кл.слова (ненормированные):
adenine -- drug derivative -- kinetin -- N benzyladenine -- N-benzyladenine -- naphthol derivative -- naphthyl acetate -- article -- chemistry -- culture medium -- culture technique -- cytology -- drug effect -- fertilization -- germination -- growth, development and aging -- methodology -- physiology -- pine -- plant -- plant seed -- Russian Federation -- zygote -- Adenine -- Cell Culture Techniques -- Culture Media -- Fertilization -- Germination -- Kinetin -- Naphthols -- Pinus -- Plant Shoots -- Seeds -- Siberia -- Zygote
Аннотация: Seedlings were grown in vitro from fertilized eggs and immature embryos of the Siberian stone pine. Cultivation of megagametophytes on a hormone-containing Murashige-Skoog medium from the egg formation until the globular embryo stage made it possible to manipulate fertilization and embryogenesis. Immature embryos are the most promising for in vitro cultivation. Their maturation and germination proceed within seven days of cultivation. When zygotic embryos were cultivated, adventitious buds were formed from cells at the cotyledon base and tips. When adventitious buds were subcultivated on a medium containing benzylaminopurine and naphthylacetic acid, organogenic callus and shoots were formed. Thus, cultivation of megagametophytes and embryos of the Siberian stone pine led to the completion of embryogenesis and formation of viable of seedlings.
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Держатели документа:
Sukachev Institute of Forestry, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia.
Доп.точки доступа:
Tret'iakova, I.N.; Novoselova, N.V.