Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ S. P. Makarenko [et al.] // Russ. J. Plant Physiol. - 2016. - Vol. 63, Is. 2. - P252-258, DOI 10.1134/S1021443716020102 . - ISSN 1021-4437
Аннотация: Fatty acid (FA) composition of total lipids in embryogenic and nonembryogenic calli of Siberian larch (Larix sibirica Ledeb.) was investigated by the method of GC-MS. We detected a high content of oleic acid in total lipids of embryogenic cell culture (32–56% by weight of total FA), which apparently depends on a high activity of acyl carrier protein (stearoyl-ACP-Δ9-desat-urase). At the same time, activity of Δ12-desaturase in the cells of embryogenic calli was considerably (2–3 times) lower than in nonembryogenic calli. We discuss a possibility that concentration of FA (oleic and linoleic acids) may be used as a marker of embryogenic potential when promising cell lines of Siberian larch are screened in the stage of early embryogenesis. © 2016, Pleiades Publishing, Ltd.

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Держатели документа:
Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, Russian Federation
Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Akademgorodok 50/28, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Makarenko, S. P.; Shmakov, V. N.; Dudareva, L. V.; Stolbikova, A. V.; Semenova, N. V.; Tret’yakova, I. N.; Konstantinov, Y. M.

/ N. V. Semenova, V. N. Shmakov, M. E. Park [и др.] // Biol. Membr. - 2020. - Vol. 37, Is. 3. - С. 215-223, DOI 10.31857/S0233475520020127. - Cited References:41 . - ISSN 0233-4755РУБ Cell Biology

Аннотация: It was found that glycerides were dominant for both types of cell lines. It was found that triglycerides (TG) and 1,2-diglycerides (1,2-DG) accumulated 1.5-3 times more actively in embryogenic cell lines, which was probably connected with the necessity of the involvement of TG and 1,2-DG in the processes of tissue differentiation. A high relative content of free fatty acids (FFA) was also characteristic of the embryogenic lines. The found differences in the ratio of sterols to their ethers (the content of sterol esters was 1.5-3 times lower in embryogenic cell lines) can indicate that sterols get activated from their inert form to contribute to the embryogenesis processes at a stage of embryo formation.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Siberian Inst Plant Physiol & Biochem, Irkutsk 664033, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Semenova, N., V; Shmakov, V. N.; Park, M. E.; Tretyakova, I. N.; Konstantinov, Yu M.; Dudareva, L., V

/ Zh. A. Koh, Yu. A. Litovka, P. V. Makolova [и др.] // Khimiya Rastitel'nogo Syr'ya. - 2021. - Is. 4. - С. 395-403, DOI 10.14258/JCPRM.2020048530 . - ISSN 1029-5151
   Перевод заглавия: Биохимический состав вегетативных эксплантов и каллусов pinus sibirica du tour
Аннотация: The methods of sterilization of annual shoots Pinus sibirica Du Tour and the conditions for their introduction into in vitro culture were studied. Induction of callusogenesis of aseptically viable explants of P. sibirica proceeds more intensively on the modified Murasige-Skoog medium: hormonal supply of 0.4% kinetin and 0.25% 6-benzylaminopurine; reduced sucrose concentration of 1.5%. The frequency of callus formation was 83%. Close quantitative indicators of extractive substances were established (36 and 33% of absolutely dry weight for callus and explant, respectively); easily hydrolyzable polysaccharides (18 and 16%) and proteins (11 and 10%).Callus P. sibirica has a higher content of ascorbic acid, flavanoids, tocopherols and ash elements compared to explants and a low amount of hard-hydrolyzable polysaccharides, lipids, tannins, pigments, and essential oils.The electrophoretic spectrum of water-soluble callus proteins is represented by eleven fractions: 63% of the total water-soluble proteins are fractions with a molecular weight of 33 kD and above. Fractions with molecular weights of 50 and 62 kD (20 and 17%, respectively) are represented as much as possible.In the explants of P. sibirica, low molecular weight fractions of proteins with molecular masses of 5 kD and lower (59%) predominate. The amino acid composition of calli and explants of P. sibirica is identical and is represented by fifteen individual amino acids. Callus tissue has a higher content of glutamic acid and two hydrophobic amino acids (proline and isoleucine) compared to the vegetative part of the plant and low tyrosine content. © 2020 Altai State University. All rights reserved.

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Держатели документа:
Institute of Forest Named After V.N. Sukachev, SB, RAS, FRC, KSC, SB, RAS, Akademgorodok, 50/28, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agrarian University, pr. Mira, 90, Krasnoyarsk, 660049, Russian Federation
Siberian State University of Science and Technology Named After Academician M.F. Reshetneva, pr. Mira, 82, Krasnoyarsk, 660049, Russian Federation

Доп.точки доступа:
Koh, Zh. A.; Litovka, Yu. A.; Makolova, P. V.; Shabanova, K. A.; Pavlov, I. N.