Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / G. G. Polyakova, V. V. Stasova, N. V. Pashenova // Russ. J. Plant Physiol. - 2011. - Vol. 58, Is. 5. - P819-827, DOI 10.1134/S1021443711050177. - Cited References: 23. - This work was supported by the Russian Foundation for Basic Research, project no. 09-04-09030. . - 9. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Ophiostomatoid fungi colonize the conducting tissues of conifer stems, the phloem and the xylem. These pathogenic fungi penetrate into the stem through injuries made by xylophagous insects vectoring these pathogens. In this study the response of the phloem of Scotch pine (Pinus sylvestris L.) to wounding (treatment 1) was compared with the response to wounding combined with application of high-molecular-weight compounds isolated from the mycelium of the ophiostomatoid fungus Ceratocystis laricicola Redfern & Minter (treatment 2). Both treatments induced the appearance of necrosis in the inner bark, the formation of periderm separating living and dead tissues, and formation of the callus alongside the wound perimeter. In addition, the bark accumulated lignin, bound proanthocyanidins, and resins, with a parallel decrease in the content of free proanthocyanidins, low-molecular-weight carbohydrates, and non-lignin components of the cell wall (P > 0.95). The size of necrotic spots, as well as changes in the content of most substances, were significantly higher in the treatment 2 than in the treatment 1 (P > 0.95). The accumulation of lignin in cell walls of phloem sieve cells was delayed in the treatment 2 as compared with that in the treatment 1. This suggested that the mycelial extract temporarily inhibited lignification at the early stage of the wound response. This disturbance of the cell wall protective transformation led to the hypothesis that the fungal suppressors retard the repair of inner bark injured by insects, thereby favoring the invasion of conifer tissues by ophiostomatoid fungi.

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Держатели документа:
[Polyakova, G. G.
Stasova, V. V.
Pashenova, N. V.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Stasova, V.V.; Pashenova, N.V.

[Text] / G. G. Polyakova [et al.] // Russ. J. Plant Physiol. - 2008. - Vol. 55, Is. 4. - P496-502, DOI 10.1134/S1021443708040109. - Cited References: 25 . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: The elicitor activity of compounds extracted from the mycelia of six species of phytopathogenic fungi was assessed from the sizes of necrotic lesions on the external surface of the living trunk phloem of five coniferous species inhabiting Siberia: Siberian larch (Larix sibirica L.), Scotch pine (Pinus sylvestris L.), Siberian spruce (Picea obovata Ledeb.), Siberian fir (Abies sibirica L.), and cedar pine (Pinus sibirica (Rupr.) Mayr.). The compounds for inoculation were extracted from the mycelium of ascomycetes imperfect, and basidium fungi; the living mycelia of these fungi were also used. The fungal extract or mycelium was placed into the hollows 7 mm in diameter in the trunk bark. Infection triggered the formation of hypersensitivity necrotic lesions in the inner bark exceeding in size those appeared after control wounding of four tree species (larch, pine, spruce, and cedar); fir was an exclusion. In experiments with tree trunks and conifer calluses, a dependence of immune response parameters (the sizes of necrotic lesions and the content of lignin and bound proanthocyanidins) on the quantity of the fungal preparation was elucidated. The largest necrotic lesions appeared after injection of 500 mu g of the fungal preparation into the hollow in the trunk, and its higher quantities did not increase the indices measured. The size of the necrotic lesion on the trunk bark is supposed to be used as a promising index characterizing the level of tree immunity and tolerance under various ecological conditions.

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Держатели документа:
[Polyakova, G. G.
Pashenova, N. V.
Polyakov, V. I.
Zrazhevskaya, G. K.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Pashenova, N.V.; Polyakov, V.I.; Zrazhevskaya, G.K.

[Text] / I. N. Tretyakova, E. V. Bazhina // Ekol. Bratisl. - 2000. - Vol. 19, Is. 3. - P280-294. - Cited References: 49 . - 15. - ISSN 1335-342XРУБ Ecology

Аннотация: Structure of crown and viability of generative organs of Siberian fir were studied in disturbed forest ecosystems near Baikal Lake. The crown structure changes in damaged trees: a zone of subapical drying appears and apical dominance is lost. Needle chlorosis and necrosis appear and proceed in sprouts of male sexualization. The tree top including the female generative organs does not show degradation symptoms during about 30 years and produces seed cones. Processes of degradation in male generative zone greatly influence on fir tree pollen. Pollen viability of fir trees varied from year to year in different trees but always pollen quality of trees growing in the Khamar-Daban Mts in the air pollution zone was more poor in comparison with the pollen of trees at die Baikal Lake shore. Tree pollen growing in the Khamar-Daban Mts germinates in vitro but pollen tubes are formed short and not viable. Seed quality of fir trees is very low, A lot of seeds are sterile. Empty seeds prevail. The formation of empty seeds was caused by the low pollen germination in ovules and as a whole absence of pollen in ovules. The high sterility of generative organs and especially of Abies sibirica pollen can limit gametic selection and decrease fir diversity in forest ecosystems near Baikal Lake.

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Держатели документа:
RAS, SB, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Tretyakova, I.N.; Bazhina, E.V.

/ N. -S. Cho [et al.] // Journal of the Faculty of Agriculture, Kyushu University. - 2008. - Vol. 53, Is. 2. - P395-398 . - ISSN 0023-6152
Аннотация: A method for estimation of tree's condition on activity of defence response in phloem was checked in Pinus sylvestris L. stands located near Krasnoyarsk (Siberia, Russia). The length of necrosis caused by inoculation of phloem of living tree by Ceratocystis laricicola Redf. et Minter was used as the parameter being measured. The field experiments were carried out in two even-aged (about 60 years) pine stands that were approximately equal on structure, productivity, density and recreation loading, but differentiated on degree of industrial pollution. The two permanent sample plots (SP) were founded in the severely polluted stand. The other two SP were placed in the unpolluted pine forest. The amount of pine trees within each SP varied from 200 to 250. From 22 up to 37 pine-trees selected randomly within every SP were inoculated with C. laricicola mycelium (test 1) and its extract (test 2). One inoculation hole per one tree (diameter 7 mm) was made in stem at a height of 1.3 m. Application of the both agents caused necrosis in phloem around the place of inoculation. In the case of fungal inoculation (test 1), the average length of necrosis in the unpolluted forest exceeded significantly the same parameter in the polluted stand: 51.7-79.4mm and 39.4-41.3mm, correspondingly. The action of the fungal metabolites caused the opposite results: in the unpolluted stand the average size of necroses was smaller in comparison with this parameter in the polluted stands 44.5-15.3 mm and 57.9-61.8 mm. The reasons of this difference are discussed. The both agents (C. laricicola mycelium and its extract) were suitable to reveal the difference of tree's condition in polluted and unpolluted pine stands. The application of fungal extract is more preferable in comparison with fungal mycelium because of smaller variability of necrosis size. Besides, the application of extract allows controlling inoculum dose and excludes the dangerous of spreading infection in forests.

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Держатели документа:
Department of Forest and Forest Products Sciences, Laboratory of Forest Resources Management, Division of Forest Ecosphere Management, Sasaguri, Fukuoka 811-2415, Japan
Wood and Paper Science, Chungbuk National University, Cheongju 361-763, South Korea
Department of Physical and Chemical Biology and Biotechnology of Woody Plants, V. N. Sukachev Institute of Forest SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Cho, N.-S.; Pashenova, N.V.; Choi, T.-H.; Ohga, S.

[Text] / S. R. Kuzmin, N. A. Kuzmina // Russ. J. Ecol. - 2015. - Vol. 46, Is. 2. - P209-212, DOI 10.1134/S1067413615010087. - Cited References:8. - The study is supported by the Russian Foundation for Basic Research (nos. 11-04-00246-a, 11-04-00063-a and 14-04-31366) and by the Ministry of Education and Science of the Russian Federation (SFU no. 4.4290.2011). . - ISSN 1067-4136. - ISSN 1608-3334РУБ Ecology


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Держатели документа:
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
ИЛ СО РАН

Доп.точки доступа:
Kuzmin, S.R.; Kuzmina, N.A.; Russian Foundation for Basic Research [11-04-00246-a, 11-04-00063-a, 14-04-31366]; Ministry of Education and Science of the Russian Federation (SFU) [4.4290.2011]

/ I. N. Pavlov, R. Vasaitis, Y. A. Litovka [et al.] // Sci. Rep. - 2020. - Vol. 10, Is. 1. - Ст. 5597, DOI 10.1038/s41598-020-62566-y . - ISSN 2045-2322
Аннотация: During recent years, a new disease of Siberian fir (A. sibirica) emerged in Central Siberia, exhibiting symptoms of stem/branch deformation, cambium necrosis, and dieback of branches and twigs, the causal agent remaining unknown. The aim was to identify agent of the disease and to investigate its pathogenicity to A. sibirica and Norway spruce (Picea abies). Symptomatic tissues of fir were subjected to pure culture isolation of anticipated pathogen(s). Obtained isolates were subjected to molecular identification, phylogenetic analyses, and pathogenicity tests with A. sibirica saplings, and seeds and seedlings of A. sibirica and P. abies. The study demonstrated that, (i) most commonly isolated fungus from canker wounds of A. sibirica exhibited Acremonium-like anamorphs; (ii) phylogeny demonstrated that investigated fungi belong to genus Corinectria, but are genetically well separated from other worldwide known Corinectria spp.; (iii) one species of isolated fungi has the capacity to cause the disease and kill A. sibirica saplings and seedlings, but also seedlings of P. abies. Guidelines for future research were defined in order to generate needed information on species description, its origin and ecology, and estimation of potential risks upon the eventual invasion of the pathogen to new geographic areas, in particular of Europe. © 2020, The Author(s).

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Держатели документа:
V.N. Sukachev Institute of Forest SB RAS, Laboratory of Reforestation, Mycology and Plant Pathology, Krasnoyarsk, 660036, Russian Federation
Reshetnev Siberian State University of Science and Technology, Department of Chemical Technology of Wood and Biotechnology, Krasnoyarsk, 660037, Russian Federation
Swedish University of Agricultural Sciences (SLU), Department of Forest Mycology and Plant Pathology, P.O. Box 7026, Uppsala, SE-75007, Sweden
Mendel University in Brno, Department of Forest Protection and Wildlife Management Zemedelska 3, Brno, 61300, Czech Republic

Доп.точки доступа:
Pavlov, I. N.; Vasaitis, R.; Litovka, Y. A.; Stenlid, J.; Jankovsky, L.; Timofeev, A. A.; Menkis, A.

/ I. N. Pavlov, R. Vasaitis, Y. A. Litovka [et al.] // Sci Rep. - 2020. - Vol. 10, Is. 1. - Ст. 5597, DOI 10.1038/s41598-020-62566-y. - Cited References:28. - Financial support from the Swedish Research Council Formas (project no. 2019-00597) is gratefully acknowledged. R. Vasaitis acknowledges the support by the EU European Structural and Investment Funds, Operational Programme Research, Development and Education, (OP RDE project "MENDELU international development", reg. No. CZ.02.2.69/0.0/0.0/16_027/0007953), and the Ministry of Education, Youth and Sports of the Czech Republic. Open access funding provided by Swedish University of Agricultural Sciences. . - ISSN 2045-2322РУБ Multidisciplinary Sciences

Аннотация: During recent years, a new disease of Siberian fir (A. sibirica) emerged in Central Siberia, exhibiting symptoms of stem/branch deformation, cambium necrosis, and dieback of branches and twigs, the causal agent remaining unknown. The aim was to identify agent of the disease and to investigate its pathogenicity to A. sibirica and Norway spruce (Picea abies). Symptomatic tissues of fir were subjected to pure culture isolation of anticipated pathogen(s). Obtained isolates were subjected to molecular identification, phylogenetic analyses, and pathogenicity tests with A. sibirica saplings, and seeds and seedlings of A. sibirica and P. abies. The study demonstrated that, (i) most commonly isolated fungus from canker wounds of A. sibirica exhibited Acremonium-like anamorphs; (ii) phylogeny demonstrated that investigated fungi belong to genus Corinectria, but are genetically well separated from other worldwide known Corinectria spp.; (iii) one species of isolated fungi has the capacity to cause the disease and kill A. sibirica saplings and seedlings, but also seedlings of P. abies. Guidelines for future research were defined in order to generate needed information on species description, its origin and ecology, and estimation of potential risks upon the eventual invasion of the pathogen to new geographic areas, in particular of Europe.

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Держатели документа:
VN Sukachev Inst Forest SB RAS, Lab Reforestat Mycol & Plant Pathol, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Dept Chem Technol Wood & Biotechnol, Krasnoyarsk 660037, Russia.
Swedish Univ Agr Sci SLU, Dept Forest Mycol & Plant Pathol, POB 7026, SE-75007 Uppsala, Sweden.
Mendel Univ Brno, Dept Forest Protect & Wildlife Management, Zemedelska 3, Brno 61300, Czech Republic.

Доп.точки доступа:
Pavlov, Igor N.; Vasaitis, Rimvydas; Litovka, Yulia A.; Stenlid, Jan; Jankovsky, Libor; Timofeev, Anton A.; Menkis, Audrius; Timofeev, Anton; Swedish Research Council FormasSwedish Research CouncilSwedish Research Council Formas [2019-00597]; EU European Structural and Investment Funds, Operational Programme Research, Development and Education, (OP RDE project "MENDELU international development") [CZ.02.2.69/0.0/0.0/16_027/0007953]; Ministry of Education, Youth and Sports of the Czech RepublicMinistry of Education, Youth & Sports - Czech Republic; Swedish University of Agricultural Sciences

[Текст] / Н. В. Пашенова, Л. Г. Серая, Ю. Н. Баранчиков // Сибирский лесной журнал. - 2023. - № 1. - С. 58-69, DOI 10.15372/SJFS20230106 . - ISSN 2311-1410

ГРНТИ

68

68.47.37

34.33.19

Аннотация: Лабораторный метод изучения грибной фитотоксичности на высечках из листьев апробирован на грибе Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz & Hosoya - возбудителе усыхания ясеня ( Fraxinus L.). Использовали 12 культур гриба, происходящие из исходного и инвазийного ареалов возбудителя, и листья ясеней маньчжурского ( Fraxinus mandshurica Rupr.) и пенсильванского ( F. pennsylvanica Marsh.), различающихся по устойчивости к данному фитопатогену. После роста грибов на жидких питательных средах фильтраты культур наносили на высечки из листьев ясеней, помещенные во влажные камеры. Некротизация фотосинтезирующих тканей отмечена после действия экзометаболитов некоторых культур. Крупные некрозы развивались только на высечках из листьев ясеня пенсильванского. Это свидетельствует о том, что данный вид менее устойчив к H. fraxineus по сравнению с ясенем маньчжурским. Географическое происхождение и состав питательной среды не влияли на способность культур вызывать некроз. Анализ результатов указывал на вероятную положительную связь между некротизирующей активностью культуральной жидкости и уровнем урожайности биомассы. Можно предположить, что факторы, индуцирующие некроз, появились в культурах на стационарной стадии роста гриба. Не обнаружено совпадения результатов лабораторных опытов с листовыми высечками и полевых опытов по инокуляции мицелия H. fraxineus в стволы молодых ясеней. Обсуждается дефицит знаний о физиологии H. fraxineus и механизмах взаимодействия этого фитопатогена с хозяином. Сделан вывод о пригодности лабораторного метода с использованием высечек из листьев для изучения факторов фитопатогенности H. fraxineus , действующих при заселении фотосинтетической части кроны у чувствительных видов ясеня
A laboratory method for studying the fungal phytotoxicity with cut-offs from leaves was tested for the fungus Hymenoscyphus fraxineus the causative agent of ash dieback ( Fraxinus L.) disease. We used 12 cultures of the fungus originating from the native and invasive ranges of the pathogen, and leaves of two species of ash Manchurian ( Fraxinus mandshurica Rupr.) and ash green ( F. pennsylvanica Marsh.) that differed in resistance to this phytopathogen. After cultivation of fungi on liquid nutrient media, the cultural filtrates were applied to cut-offs from ash leaves placed in moist chambers. Necrotization of photosynthetic tissues was noted after the action of exometabolites of some cultures. At the same time, large necrosis have developed only on the cut-offs from the leaves of ash green, which corresponds to the known fact that this species is less resistant to H. fraxineus in comparison with of ash Manchurian. The geographical origin and composition of the culture medium did not affect the ability of cultures to induce necrosis. The analysis of the results indicated a probable positive relationship between the necrotizing activity of the culture liquid and the indicators of the crop biomass yield. It can be assumed that the necrosis inducing factors appeared in cultures at the stationary stage of the fungus growth. No concurrence was found between the results of laboratory tests with leaf cut-offs and field experiments on the inoculation of H. fraxineus mycelium into the trunks of young ash trees. The deficiency of knowledge about the H. fraxineus physiology and the mechanisms of interaction of this phytopathogen with the host are discussed. A conclusion was made about the suitability of the laboratory technique with leaf cut-offs for factors of H. fraxineus phytopathogenicity investigation, which act during the colonization of the photosynthetic part of the crown in sensitive ash species

РИНЦ

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Серая, Лидия Георгиевна; Seraya, Lidiya Georgievna; Баранчиков, Юрий Николаевич; Baranchikov, Yury Nikolayevich; Pashenova, Natal'ya Veniaminovna