Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ I. N. Tretyakova [et al.] // Russ. J. Dev. Biol. - 2018. - Vol. 49, Is. 6. - P370-380, DOI 10.1134/S1062360419010053. - Cited References:18. - The study was financially supported by the Russian Foundation for Basic Research, the Government of Krasnoyarsk krai, and the Krasnoyarsk Regional Science Foundation (project nos. 16-44-240509 and 18-44-243004). . - ISSN 1062-3604. - ISSN 1608-3326РУБ Developmental Biology

Аннотация: In vitro cultivation of embryogenic cultures of Siberian larch on AI medium supplemented with different concentrations of antimicrobial peptides of two Trichoderma species, T.citrinoviride (strain TYVI 4/11) and T. viride (strain 346), has been performed to achieve a direct antimicrobial effect and initiate the mechanisms of induced resistance (regulatory function of embryogenic cultures) as well as to study the morphogenesis and growth activity of regenerant plants. The experiment was arranged using four cell lines, CL4, CL5, CL6, and CL12, differing in their embryogenic activity. The effect of treatment of cell cultures with Trichoderma peptides was manifested via significant growth stimulation of proliferating embryogenic cell lines, root growth stimulation, and formation of callus excrescences on regenerant plants. No difference between the treated and untreated variants was observed in seedlings. Conceivably, such peptide treatment may provide an increased immunity of soil-grown seedlings in forest nurseries. Study of the effect of biocontrol strains' use in a plantation reforestation to stimulate the growth and development of calluses and embryos and to obtain disease-resistant regenerant plants of conifer species will provide the development of a clonal silviculture, a new trend that recently appeared abroad (Park 2002, 2014).

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Держатели документа:
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Gauze Inst New Antibiot, Moscow 119021, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia.

Доп.точки доступа:
Tretyakova, I. N.; Park, M. E.; Baranova, A. A.; Lisetskaya, I. A.; Shuklina, A. S.; Rogozhin, E. A.; Sadykova, V. S.; Russian Foundation for Basic Research; Government of Krasnoyarsk krai; Krasnoyarsk Regional Science Foundation [16-44-240509, 18-44-243004]

/ I. N. Tretyakova, E. A. Rogozhin, M. E. Pak [et al.] // Biol. Bull. - 2020. - Vol. 47, Is. 3. - P225-236, DOI 10.1134/S1062359020030097. - Cited References:38. - This work was conducted within the framework of a Budget Project of Sukachev Institute of Forests, Siberian Branch, Russian Academy of Sciences, Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences"(project no. 0356-2017-0741) and supported by the Russian Foundation for Basic Research, the Government of Krasnoyarsk region, and the Krasnoyarsk Regional Science Foundation within the framework of research project no. 16-44-240509 "The Development of Biotechnology for the Production of Embryogenic Cultures of Siberian Larch Resistant to Fungal Diseases and Pests with the Use of Protective Antimicrobial Peptides in vitro" and no. 18-44-243004 "In vitro Studies of the Effect of Biologically Active Peptides of Plant and Microbial Origin on the Growth and Development of Conifers in Early Ontogenesis." . - ISSN 1062-3590. - ISSN 1608-3059РУБ Biology

Аннотация: The effect of plant antimicrobial peptides on the initiation of callus and embryonal suspensor masses, formation of somatic embryos, and germination of regenerants of Siberian larch has been studied. Protein/peptide extracts isolated fromAmarantus retroflexus(seeds),Nigella sativa(seeds), andElytrigia elongata(spikelets) have been used as objects of plant origin. Peptides have been introduced into the nutrient media at the stage of initiation of embryogenic cultures and somatic embryo germination. The stimulating effect of peptides on the formation of embryogenic cultures of Siberian larch has been found. No other differences in the dynamics of growth in the control and experimental regenerants have been observed. This study is supposed to contribute to enhancing the immunity of the clonal planting stock of Siberian larch.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forests, Siberian Branch, Div Fed Res Ctr Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia.
Gause Inst New Antibiot, Moscow 119021, Russia.
Tyumen State Univ, Tyumen 625003, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Tretyakova, I. N.; Rogozhin, E. A.; Pak, M. E.; Petukhova, I. A.; Shuklina, A. S.; Pahomova, A. P.; Sadykova, V. S.; Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences [0356-2017-0741]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Government of Krasnoyarsk region; Krasnoyarsk Regional Science Foundation [16-44-240509, 18-44-243004]

/ I. N. Tretyakova, E. A. Rogozhin, M. E. Pak [et al.] // Biol. Bull. - 2020. - Vol. 47, Is. 3. - P225-236, DOI 10.1134/S1062359020030097 . - ISSN 1062-3590
Аннотация: Abstract: The effect of plant antimicrobial peptides on the initiation of callus and embryonal suspensor masses, formation of somatic embryos, and germination of regenerants of Siberian larch has been studied. Protein/peptide extracts isolated from Amarantus retroflexus (seeds), Nigella sativa (seeds), and Elytrigia elongata (spikelets) have been used as objects of plant origin. Peptides have been introduced into the nutrient media at the stage of initiation of embryogenic cultures and somatic embryo germination. The stimulating effect of peptides on the formation of embryogenic cultures of Siberian larch has been found. No other differences in the dynamics of growth in the control and experimental regenerants have been observed. This study is supposed to contribute to enhancing the immunity of the clonal planting stock of Siberian larch. © 2020, Pleiades Publishing, Inc.

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Держатели документа:
Sukachev Institute of Forests, Siberian Branch, Russian Academy of Sciences, Division of Federal Research Center “Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences”, Krasnoyarsk, 660036, Russian Federation
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russian Federation
Gause Institute of New Antibiotics, Moscow, 119021, Russian Federation
Tyumen State University, Tyumen, 625003, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Tretyakova, I. N.; Rogozhin, E. A.; Pak, M. E.; Petukhova, I. A.; Shuklina, A. S.; Pahomova, A. P.; Sadykova, V. S.