Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ I. N. Pavlov, Y. A. Litovka, D. V. Golubev [et al.] // Contemp. Probl. Ecol. - 2020. - Vol. 13, Is. 1. - P71-84, DOI 10.1134/S1995425520010060. - Cited References:59 . - ISSN 1995-4255. - ISSN 1995-4263РУБ Ecology

Аннотация: Conifer decline (Abies sibirica Ledeb. stands) as a result of complex biotic effects on the territory of Krasnoyarsk krai by 2018 reached an area of 541 400 ha; the potential area of further death of A. sibirica is up to 10 million ha. The main reason is the phytopathogenic fungi Armillaria mellea s.l. and Heterobasidion annosum s.l. combined with bark beetle Polygraphus proximus. Trees affected by root pathogens are centers of forest dieback, from which pathological outbreaks spread, including the effects of P. proximus and its phytopathogenic mycoassociates. Neonectria fuckeliana (C. Booth) Castl & Rossman is among the concomitant pathogens in the scale of the lesion, which causes a resin flow that is similar to the effects of P. proximus attack. The phytopathogenicity of pure cultures of the dominant root pathogens of Armillaria borealis Marxm. & Korhonen and Heterobasidion annosum (Fr.) Bref., as well as the stem pathogen of N. fuckeliana, has been confirmed by a series of experiments in vitro. Promising natural agents for the regulation of P. proximus abundance are highly virulent aboriginal strains of Beauveria bassiana (Bals.-Criv.) Vuill., resulting in maximum insect death (up to 100%) for 7-11 days and retaining activity in the temperature range of 16-24 +/- 1 degrees C.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660037, Russia.
Federal Forestry Agcy, Russian Ctr Forest Hlth Branch, Ctr Forest Hlth Krasnoyarsk Krai, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Pavlov, I. N.; Litovka, Y. A.; Golubev, D. V.; Astapenko, S. A.; Chromogin, P. V.; Usoltseva, Y. V.; Makolova, P. V.; Petrenko, S. M.

/ T. V. Antipova, V. P. Zhelifonova, Y. A. Litovka [et al.] // Appl. Biochem. Microbiol. - 2020. - Vol. 56, Is. 2. - P185-193, DOI 10.1134/S0003683820020039. - Cited References:30 . - ISSN 0003-6838. - ISSN 1608-3024РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: The composition of secondary metabolites from strains Heterobasidion genus isolated in central and western Siberia and in South Korea were studied. Morphological-cultural and molecular-genetic methods were used to assign the cultures to the species of H. annosum (Fr.) Bref. (five strains), H. abietinum Niemela & Korhonen (four strains), and H. ecrustosum Tokuda, T. Hatt. & Y.C. Give (one strain). Fomannoxin predominated in the metabolome profiles of three H. annosum strains and all H. abietinum strains. Two strains of H. annosum synthesized fomannoxin-related compounds: 2-(2-hydroxypropan-2-yl)-2,3-dihydrobenzofuran-5-carbaldehyde and 2-(2-hydroxypropan-2-yl)benzofuran-5-carbaldehyde. Fomannosin and its precursors were identified in H. annosum 45-2. It was shown that the composition of the fermentation medium affected the number and range of the synthesized metabolites. Under in vitro conditions, all H. annosum and H. abietinum strains exhibited phytopathogenic effects on Pinus sylvestris L. seedlings, causing necrotic damage to stems of various degrees and plant death. Higher phytopathogenicity is characteristic of H. annosum strains: maximal aggressiveness was observed in H. annosum 45-2 with the greatest diversity of compounds in the metabolome profile and active fomannoxin production.

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Держатели документа:
Russian Acad Sci, Pushchino Sci Ctr, Biol Res Fed Res Ctr, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Forest Inst, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660049, Russia.
RAS, SB, FRC Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Antipova, T. V.; Zhelifonova, V. P.; Litovka, Yu. A.; Pavlov, I. N.; Baskunov, B. P.; Timofeev, A. A.; Kozlovsky, A. G.; Timofeev, Anton

[Текст] / Н. В. Пашенова, Л. Г. Серая, Ю. Н. Баранчиков // Сибирский лесной журнал. - 2023. - № 1. - С. 58-69, DOI 10.15372/SJFS20230106 . - ISSN 2311-1410

ГРНТИ

68

68.47.37

34.33.19

Аннотация: Лабораторный метод изучения грибной фитотоксичности на высечках из листьев апробирован на грибе Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz & Hosoya - возбудителе усыхания ясеня ( Fraxinus L.). Использовали 12 культур гриба, происходящие из исходного и инвазийного ареалов возбудителя, и листья ясеней маньчжурского ( Fraxinus mandshurica Rupr.) и пенсильванского ( F. pennsylvanica Marsh.), различающихся по устойчивости к данному фитопатогену. После роста грибов на жидких питательных средах фильтраты культур наносили на высечки из листьев ясеней, помещенные во влажные камеры. Некротизация фотосинтезирующих тканей отмечена после действия экзометаболитов некоторых культур. Крупные некрозы развивались только на высечках из листьев ясеня пенсильванского. Это свидетельствует о том, что данный вид менее устойчив к H. fraxineus по сравнению с ясенем маньчжурским. Географическое происхождение и состав питательной среды не влияли на способность культур вызывать некроз. Анализ результатов указывал на вероятную положительную связь между некротизирующей активностью культуральной жидкости и уровнем урожайности биомассы. Можно предположить, что факторы, индуцирующие некроз, появились в культурах на стационарной стадии роста гриба. Не обнаружено совпадения результатов лабораторных опытов с листовыми высечками и полевых опытов по инокуляции мицелия H. fraxineus в стволы молодых ясеней. Обсуждается дефицит знаний о физиологии H. fraxineus и механизмах взаимодействия этого фитопатогена с хозяином. Сделан вывод о пригодности лабораторного метода с использованием высечек из листьев для изучения факторов фитопатогенности H. fraxineus , действующих при заселении фотосинтетической части кроны у чувствительных видов ясеня
A laboratory method for studying the fungal phytotoxicity with cut-offs from leaves was tested for the fungus Hymenoscyphus fraxineus the causative agent of ash dieback ( Fraxinus L.) disease. We used 12 cultures of the fungus originating from the native and invasive ranges of the pathogen, and leaves of two species of ash Manchurian ( Fraxinus mandshurica Rupr.) and ash green ( F. pennsylvanica Marsh.) that differed in resistance to this phytopathogen. After cultivation of fungi on liquid nutrient media, the cultural filtrates were applied to cut-offs from ash leaves placed in moist chambers. Necrotization of photosynthetic tissues was noted after the action of exometabolites of some cultures. At the same time, large necrosis have developed only on the cut-offs from the leaves of ash green, which corresponds to the known fact that this species is less resistant to H. fraxineus in comparison with of ash Manchurian. The geographical origin and composition of the culture medium did not affect the ability of cultures to induce necrosis. The analysis of the results indicated a probable positive relationship between the necrotizing activity of the culture liquid and the indicators of the crop biomass yield. It can be assumed that the necrosis inducing factors appeared in cultures at the stationary stage of the fungus growth. No concurrence was found between the results of laboratory tests with leaf cut-offs and field experiments on the inoculation of H. fraxineus mycelium into the trunks of young ash trees. The deficiency of knowledge about the H. fraxineus physiology and the mechanisms of interaction of this phytopathogen with the host are discussed. A conclusion was made about the suitability of the laboratory technique with leaf cut-offs for factors of H. fraxineus phytopathogenicity investigation, which act during the colonization of the photosynthetic part of the crown in sensitive ash species

РИНЦ

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Серая, Лидия Георгиевна; Seraya, Lidiya Georgievna; Баранчиков, Юрий Николаевич; Baranchikov, Yury Nikolayevich; Pashenova, Natal'ya Veniaminovna