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Найдено документов в текущей БД: 7

    Microbial polymers as a degradable carrier for pesticide delivery
[Text] / O. N. Voinova [et al.] // Appl. Biochem. Microbiol. - 2009. - Vol. 45, Is. 4. - P384-388, DOI 10.1134/S0003683809040061. - Cited References: 21. - This study was supported by the Ministry of Education and Science of the Russian Federation and the U.S. Civilian Research and Development Foundation grant no. P1Me002), joint program of the Russian Foundation for Basic Research and the Krasnoyarsk Krai Science Foundation (project no. 07-08-96800- r_yenisei_a), the Krasnoyarsk Krai Science Foundation (project no. 18G142), and the Russian Science Support Foundation. . - 5. - ISSN 0003-6838
РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: The possibility of use of polyhydroxyalkanoates (PHAs), biodegradable microbial polyesters, as a carrier for pesticides (alpha-hexachlorcyclohexane and lindane) for targeted and controlled delivery of these compounds to soil was investigated. The kinetics of polymer degradation and the dynamics of pesticide release from the extended-release formulations was studied. It is shown that pesticides embedded in a degradable polymer (PHA) carrier are released gradually and slowly, without surges, as the polymer is degraded by the soil micro-flora. The microbial soil component actively responded to the addition of the polymer as an additional nutrient substrate: the latter was degraded and then utilized. The rate of the pesticide release to the soil can be regulated by varying the polymer-pesticide ratio.

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Держатели документа:
[Voinova, O. N.
Kalacheva, G. S.] Akademgorodok, Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
[Volova, T. G.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Grodnitskaya, I. D.] Akademgorodok, Russian Acad Sci, Siberian Branch, Inst Forestry, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Voinova, O.N.; Kalacheva, G.S.; Grodnitskaya, I.D.; Volova, T.G.; Ministry of Education and Science of the Russian Federation; U.S. Civilian Research and Development Foundation [P1Me002]; Russian Foundation for Basic Research; Krasnoyarsk Krai Science Foundation [07-08-96800- r_yenisei_a, 18G142]; Russian Science Support Foundation
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    Destruction of Oil by Immobilizovannoy Mikrofloroy
/ T. V. Ryazanova, O. S. Fedorova, S. R. Loskutov // J. Sib. Fed. Univ.-Chem. - 2018. - Vol. 11, Is. 2. - С. 184-196, DOI 10.17516/1998-2836-0067. - Cited References:17 . - ISSN 1998-2836. - ISSN 2313-6049
РУБ Chemistry, Multidisciplinary

Кл.слова (ненормированные):
bioremediation -- sorption -- hydrocarbon-oxidizing microorganisms -- immobilization -- biosorbent

Аннотация: In model experiments shows the efficiency immobilized oil-degrading bacteria when the concentration of contaminant in soil 5 and 15 % (vol). As immobilizing agent used, the polymer sorbent of the type "Unisorb". Using the method of chromato-mass-spectrometry we have determined the changes of the component composition of oil in the process of bioremediation. It is shown that for the nine weeks of exposure at 5 % (about) of the contaminant in the soil disposal of oil was 95 %, while 15 % (about) - 86 %. What testifies to high efficiency of the proposed method of destruction of oil using immobilized microflora - sorbent "Unisorb-Bio". The obtained results allow to recommend it for combating oil and create backup reserves in case of emergencies.

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Держатели документа:
Reshetnev Siberian State Univ Sci & Technol, 31 Krasnoyarsky Rabochy, Krasnoyarsk 660037, Russia.
RAS, Krasnoyarsk Sci Ctr, NV Sukachev Inst Forest, FRC,SB, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ryazanova, Tatyana V.; Fedorova, Olga S.; Loskutov, Sergy R.
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    FTIR spectroscopy of early and latewood of Larix gmelinii growing along the polar treeline: the correlation between absorption bands and climatic factors
/ E. A. Tyutkova, S. R. Loskutov, N. P. Shestakov // Wood Mater. Sci. Eng. - 2019, DOI 10.1080/17480272.2018.1562495 . - Article in press. - ISSN 1748-0272
Аннотация: This paper presents the results of FTIR spectroscopy of the 1988–1998 annual ring early and latewood of Larix gmelinii (Rupr.) Rupr.) found along the polar treeline (Taimyr Peninsula, 70°52?53? N, 102°58?26? E). We analyzed samples of early and latewood to identify absorption bands of groups of wood components, as well as the bands that characterized the interactions among the components. We studied bound water spectral characteristics for wood formed in different years. An analysis of the correlation between the values of spectral absorption bands and mean monthly air temperature and precipitation showed that May-August weather had the greatest influence on the synthesis of the main polymeric components of early and latewood. Air temperature had a considerable effect on polymer composition of cell walls forming in early and latewood, whereas precipitation influenced only earlywood. FTIR spectroscopy is a promising tool to develop information on the biochemical composition of the walls of early and late tracheids of annual rings and on weather and climate influences on cell wall synthesis. © 2018, © 2019 Informa UK Limited, trading as Taylor & Francis Group.

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Держатели документа:
V.N. Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Physics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Tyutkova, E. A.; Loskutov, S. R.; Shestakov, N. P.
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    Changes in lignin structure during earlywood and latewood formation in Scots pine stems
/ G. F. Antonova [et al.] // Wood Sci Technol. - 2019. - Vol. 53, Is. 4. - P927-952, DOI 10.1007/s00226-019-01108-w . - ISSN 0043-7719
Аннотация: Lignification of earlywood and latewood during annual ring formation in Scots pine stem in the season occurs with opposite dynamics. The subject of this study was to examine the structure of lignin deposited at successive stages of secondary cell wall maturation of these two wood types. Lignin specimens derived from developing cells by the thioglycolic acid were analyzed by the methods of alkaline oxidation, alkaline and acid hydrolysis and IR Fourier spectroscopy. The composition of lignin structural subunits was found to change at each stage of lignification in dependence of the forming wood type. The molar ratio of p-hydroxyphenyl, guaiacyl and syringyl subunits in polymer was modified in earlywood and latewood oppositely. In the course of earlywood cell maturation, syringyl subunits in lignin increased in parallel with p-hydroxyphenyl units. During latewood lignification, syringyl and p-hydroxyphenyl subunits in lignin structure decreased, whereas guaiacyl subunits increased as secondary cell wall maturation. In lignin isolated from both wood types, the ether-bound carbohydrates were more than by an order of magnitude of ester-bound carbohydrates. In early xylem, the content of ether-bound carbohydrates practically did not change during lignin deposition, whereas carbohydrates with ester linkages gradually decreased toward mature xylem. Lignification in latewood was accompanied by the decline of both ester-linked and ether-linked carbohydrates with a sharp increase in the latter in mature xylem. Hemicelluloses, included in such bonds, contained mainly the residues of xylose and arabinose. The composition and the content of hydroxycinnamic acids, taking part in these linkages, also changed in dependence of lignification steps and wood type. The data received are in agreement with the alterations in absorption IR spectra of lignins isolated from the cells at the beginning of lignification and mature xylem. The reasons for the differences in the lignin structure during earlywood and latewood development are discussed. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.

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Держатели документа:
VN Sukachev Institute of Forest, SB RAS Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Solid State Physics RAS, 2 Academician Ossipyan Str., Chernogolovka, Moscow District, 142432, Russian Federation

Доп.точки доступа:
Antonova, G. F.; Varaksina, T. N.; Zheleznichenko, T. V.; Bazhenov, A. V.
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    FTIR spectroscopy of early and latewood of Larix gmelinii growing along the polar treeline: the correlation between absorption bands and climatic factors
/ E. A. Tyutkova, S. R. Loskutov, N. P. Shestakov // Wood Mater. Sci. Eng. - 2020. - Vol. 15, Is. 4. - P205-212, DOI 10.1080/17480272.2018.1562495. - Cited References:33 . - ISSN 1748-0272. - ISSN 1748-0280
РУБ Materials Science, Paper & Wood

Аннотация: This paper presents the results of FTIR spectroscopy of the 1988-1998 annual ring early and latewood of Larix gmelinii (Rupr.) Rupr.) found along the polar treeline (Taimyr Peninsula, 70 degrees 52' 53 '' N, 102 degrees 58'26 '' E). We analyzed samples of early and latewood to identify absorption bands of groups of wood components, as well as the bands that characterized the interactions among the components. We studied bound water spectral characteristics for wood formed in different years. An analysis of the correlation between the values of spectral absorption bands and mean monthly air temperature and precipitation showed that May-August weather had the greatest influence on the synthesis of the main polymeric components of early and latewood. Air temperature had a considerable effect on polymer composition of cell walls forming in early and latewood, whereas precipitation influenced only earlywood. FTIR spectroscopy is a promising tool to develop information on the biochemical composition of the walls of early and late tracheids of annual rings and on weather and climate influences on cell wall synthesis.

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Держатели документа:
Russian Acad Sci, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk, Russia.
Russian Acad Sci, Inst Phys, Siberian Branch, Krasnoyarsk, Russia.

Доп.точки доступа:
Tyutkova, E. A.; Loskutov, S. R.; Shestakov, N. P.
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    Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
/ S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180 . - ISSN 0045-6535

Кл.слова (ненормированные):
metabolic activity -- P(3HB) bioplastic -- P(3HB) properties -- P(3HB)-degrading strains -- Siberian cryogenic soils -- structure of microbial community -- Aspergillus -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Cryogenics -- Crystallinity -- Metabolism -- Polymer films -- Reinforced plastics -- RNA -- Soils -- Aspergillus fumigatus -- Degree of crystallinity -- Microbial communities -- Nucleotide sequences -- Poly-3-hydroxybutyrate -- Polymer biodegradation -- Soil microbial community -- Surface microstructures -- Bacteria -- bacterial RNA -- fungal RNA -- mineral -- plastic -- poly(3 hydroxybutyric acid) -- polymer -- ribosome RNA -- RNA 16S -- RNA 18S -- RNA 28S -- RNA 5.8S -- abundance -- bacterium -- biodegradation -- biomass -- community structure -- concentration (composition) -- crystallinity -- fungus -- microbial community -- microstructure -- plastic -- polymer -- soil temperature -- subarctic region -- Actinobacteria -- Agrobacterium tumefaciens -- Antarctica -- Arctic -- Article -- Aspergillus fumigatus -- Aspergillus niger -- Bacilli -- Bacillus cereus -- Bacillus pumilus -- bacterial gene -- bacterium isolate -- biodegradability -- biodegradation -- biomass -- Chryseobacterium ioostei -- colony forming unit -- community structure -- concentration (parameter) -- cryogenic soil -- crystallization -- Cupriavidus necator -- ecosystem -- Escherichia coli -- Flavobacteria -- Flavobacterium -- fungal community -- fungal gene -- Fusarium fujikuroi -- Gammaproteobacteria -- green chemistry -- Lactobacterium helveticus -- metabolism -- microbial biomass -- microbial community -- molecular weight -- Mortierella alpina -- Mycobacterium -- Mycobacterium pseudoshotsii -- Nocardioides -- nucleotide sequence -- nucleotide sequence -- Paenibacillus -- Paraburkholderia -- Penicillium -- Penicillium arenicola -- Penicillium glabrum -- Penicillium lanosum -- Penicillium restrictum -- Penicillium spinulosum -- Penicillium thomii -- phylogeny -- Pseudomonas -- Rhizopus oryzae -- Rhodococcus -- RNA sequence -- Russian Federation -- soil -- soil microflora -- soil temperature -- species composition -- Stenotrophomonas -- Streptomyces -- Streptomyces prunicolor -- surface property -- temperature dependence -- thawing -- Variovorax paradoxus -- zpseudomonas lutea -- Siberia -- Aspergillus fumigatus -- Bacillus pumilus -- Bacteria (microorganisms) -- Fungi -- Penicillium thomii -- Pseudomonas sp. -- Rhodococcus sp. -- Stenotrophomonas rhizophila -- Streptomyces prunicolor -- Variovorax paradoxus

Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd

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Держатели документа:
Siberian Federal University, 79 Svobodny Pr, Krasnoyarsk, 660041, Russian Federation
V.N. Sukachev Institute of Forest, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/28 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Melnikov Permafrost Institute, SB RAS, 36 Merzlotnaya St., Yakutsk, 677010, Russian Federation

Доп.точки доступа:
Prudnikova, S. V.; Evgrafova, S. Y.; Volova, T. G.
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    Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards "green" bioplastics
/ S. V. Prudnikova, S. Y. Evgrafova, T. G. Volova // Chemosphere. - 2021. - Vol. 263. - Ст. 128180, DOI 10.1016/j.chemosphere.2020.128180. - Cited References:101. - This study (polymer synthesis and investigation) was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning", and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (investigation of polymer degradation in soils of Evenkia). . - ISSN 0045-6535. - ISSN 1879-1298
РУБ Environmental Sciences

Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.

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Держатели документа:
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, VN Sukachev Inst Forest, 50-28 Akademgorodok, Krasnoyarsk 660036, Russia.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
SB RAS, Melnikov Permafrost Inst, 36 Merzlotnaya St, Yakutsk 677010, Russia.

Доп.точки доступа:
Prudnikova, Svetlana, V; Evgrafova, Svetlana Yu; Volova, Tatiana G.; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
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