Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / I. V. Tikhonova, V. L. Semerikov // Russ. J. Ecol. - 2010. - Vol. 41, Is. 5. - P372-377, DOI 10.1134/S1067413610050024. - Cited References: 39. - The study was supported by the Krasnoyarsk Regional Science Foundation and Russian Foundation for Basic Research (project no. 07-04-96808) and the Siberian Branch of the Russian Academy of Sciences (project no. 53). . - 6. - ISSN 1067-4136РУБ Ecology

Аннотация: Polymorphism of 11 enzyme systems has been studied in dwarf Scots pine trees occurring on sand dunes and crags in two populations of southern Central Siberia. High genotypic variability of dwarfs and their similarity to normal trees in basic indices of genetic diversity have been revealed. Both populations, including normal and dwarf trees, are in the Hardy-Weinberg equilibrium. The sample of dwarf trees has proved to differ from the "norm" in showing an age-related trend toward decreased heterozygosity. The observed linkage disequilibrium at some pairs of loci can be explained by unequal contributions of trees to population reproduction or by a recent bottleneck event. The populations studied are similar to other Scots pine populations from the Asian part of the species range. Increased frequencies of some rare alleles in the populations from Tuva provide evidence for the probable presence of a glacial refugium for pine in this region.

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Держатели документа:
[Tikhonova, I. V.] Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Akademgorodok, Russia
[Semerikov, V. L.] Russian Acad Sci, Ural Branch, Inst Plant and Anim Ecol, Ekaterinburg 620144, Russia

Доп.точки доступа:
Tikhonova, I.V.; Semerikov, V.L.

[Text] / A. N. Kravchenko, A. Y. Larionova, L. I. Milyutin // Russ. J. Genet. - 2008. - Vol. 44, Is. 1. - P35-43, DOI 10.1134/S1022795408010055. - Cited References: 42 . - 9. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: Based on the analysis of 22 loci controlling allozyme variation of MDH, SKDH, 6-PGD, IDH, PEPCA, GOT, FDH, LAP, PGI, PGM, SOD, and GDH, the data on within-and among-population variation for nine cenopopulations of Siberian spruce (Picea obovata Ledeb.) located along the Yenisei mieridian, from 65 degrees 50' NL to 52 degrees 14' NL, were obtained. It was demonstrated that 86.36% of the loci, tested in the species analyzed, were polymorphic. The mean number of alleles per locus was 2.91, and the observed and expected heterozygosity constituted 0.161 and 0.168, respectively. More than 97% of total variation occurred within the populations, while the proportion of the among-population variation constituted only 2.3% (F-st =0.0230). Genetic distance (D-N) between the populations examined varied from 0.0019 to 0.0115, averaging at 0.0051. It was shown that in the part of the Siberian spruce range examined there was no close association between the level of the genetic differentiation of the populations and the geographic distance between them. It seems likely, that this finding can be associated with the fact that Siberian spruce, growing on this territory, is the intrazonal species, i.e., it is not attached to a certain forest zone, and its distribution is mostly determined by local ecological conditions.

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Держатели документа:
[Kravchenko, A. N.
Larionova, A. Ya.
Milyutin, L. I.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Kravchenko, A.N.; Larionova, A.Y.; Milyutin, L.I.

[Text] / A. Y. Larionova, N. V. Yakhneva, A. P. Abaimov // Russ. J. Genet. - 2004. - Vol. 40, Is. 10. - P1127-1133, DOI 10.1023/B:RUGE.0000044756.55722.d8. - Cited References: 32 . - 7. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: Within- and among-population diversity of Gmelin larch Larix gmelinii (Rupr.) Rupr. from Evenkia was inferred from data on 17 genes determining allozyme diversity of ten enzymes. More than 50% of the genes proved to be polymorphic. On average, each tree was heterozygous at 9.2% genes. Heterozygosity expected from the Hardy-Weinberg proportions was higher, 12.5%. A deficit of heterozygous genotypes was observed in all populations under study and attributed to inbreeding. With Wright's F statistics, average individual inbreeding was estimated at 26.6% relative to the population (F-IS) and at 27.8% relative to the species (F-IT). The greatest deficit of heterozygosity was observed for the youngest population II. Within- population variation accounted for more than 98% of the total variation, while the contribution of among-population variation was 1.66%. Genetic distance between populations varied from 0.0025 to 0.0042, averaging 0.0035.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Larionova, A.Y.; Yakhneva, N.V.; Abaimov, A.P.

[Text] / A. Y. Larionova // Russ. J. Genet. - 2002. - Vol. 38, Is. 12. - P1391-1396, DOI 10.1023/A:1021639806123. - Cited References: 34 . - 6. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: The main parameters of genetic variability have been determined in an isolated natural Scotch pine population from Chita oblast (Tsasuchei Forest) by analysis of 19 genes coding for nine enzymes: GDH, IDH, LAP, PGM, AAT, ADH, MDH, 6-PGD, and DIA. Polymorphic genes constituted 63.2% of all structural genes studied in the population at the 99% polymorphism criterion. The mean number of alleles per locus was 1.63. The observed and expected heterozygosities were 0.237 and 0.251, respectively. These estimates are close to the corresponding mean values for Scotch pine according to the data on 18 or more structural genes.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Larionova, A.Y.

/ N. V. Ekimova, E. N. Muratova, P. P. Silkin // Russian Journal of Genetics: Applied Research. - 2012. - Vol. 2, Is. 2. - P105-109, DOI 10.1134/S2079059712020037 . - ISSN 2079-0597
Аннотация: Chromosome numbers for some species of Central Asia steppe shrubs are given: Rhamnus erythroxylon Pall. (Rhamnaceae), Caragana buriatica Peschk. (Fabaceae), Amygdalus pedunculata Pall., Armeniaca sibirica (L.) Lam. (Rosaceae), Atraphaxis pungens (Bieb.) Jaub. et Spach, and A. frutescens (L.) C. Koch (Polygonaceae). Chromosome numbers of some species were determined for the first time. A comparative analysis of adaptive properties of polyploidy and diploid species has been conducted. It was established that natural polyploids are more adaptive to existence in extreme conditions. They possess a high potential for survival and are characterized by a higher level of intraspecific polymorphism, abundant flowering and fruitification, ability to propagate both via generative and vegetative means, and high seed germination. В© 2012 Pleiades Publishing, Ltd.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
V.N. Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Ekimova, N.V.; Muratova, E.N.; Silkin, P.P.

/ O. V. Kvitko, E. N. Muratova // Cell and Tissue Biology. - 2010. - Vol. 4, Is. 2. - P215-222, DOI 10.1134/S1990519X10020124 . - ISSN 1990-519X
Аннотация: Data are presented on the karyotype structure of the Siberian fir (Abies sibirica Ledeb.) from five populations of the Central Siberia. The chromosome set (2n = 24) contains seven pairs of metacentric (I-VII), four pairs of submetacentric (VIII and X-XII), and one pair of intercentric (IX) chromosomes. The morphometric parameters of the identified chromosome groups had close values in the studied populations. The variation coefficients of chromosomal parameters of the Siberian fir correspond to very low and low variability. The intraspecific chromosome polymorphism of the Siberian fir is connected mainly to variations in the number and peculiarities of the nucleolar organizing regions of chromosomes. In the territory with technogenic loading, a wide spectrum of genomic mutations of the mixoploidy type was observed in the seed off-spring of the Siberian fir; triploid seedlings, as well as rare cases of somatic reduction of chromosomes, were revealed. В© 2010 Pleiades Publishing, Ltd.

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Держатели документа:
V.N. Sukachev Institute of Forest, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kvitko, O.V.; Muratova, E.N.

/ F. D. Avrov // Genetika. - 1990. - Vol. 26, Is. 12. - С. 2191-2199 . - ISSN 0016-6758

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Держатели документа:
V.N. Sukachev Institute of Wood and Forest, Siberian Division, Academy of Sciences of the USSR, Krasnoyarsk, Russia

Доп.точки доступа:
Avrov, F.D.

[Text] / V. L. Semerikov [et al.] // Russ. J. Genet. - 2014. - Vol. 50, Is. 6. - P577-585, DOI 10.1134/S1022795414040127. - Cited References: 38. - This work was supported by the Russian Foundation for Basic Researches, project nos. 11-04-92226-Mong_a and 12-04-00062-a, and by the Program of the Ural Branch of the Russian Academy of Sciences, project no. 12-I-4-2064. The authors thank the director of the Institute for Botany, Academy of Science of Mongolia, Academician Ch. Dzhugarzhav, and the director of the branch of FBI "Roslesoashchita" Centre of Forest Protection, Altai region, A.Ya. Bondarev, for help with material collection. . - ISSN 1022-7954. - ISSN 1608-3369РУБ Genetics & Heredity

Аннотация: The variability of four microsatellite loci of chloroplast DNA was studied in 38 populations of Pinus sylvestris in the European part of Russia, in Siberia, Kazakhstan, Transbaikalia and Mongolia. High variability was observed in all regions. In total, 152 haplotypes were identified. The level of population differentiation R (ST) was 2.1%. The differentiation of three geographical groups of populations (European, Siberian-Kazakhstan and Transbaikalian-Mongolian) was insignificant (R (CT) = 0.004). At the same time, some rare haplotypes were found to be specific for a certain geographical region. Distribution of the rare haplotypes, which differentiated European populations from Asian populations and Mongolian and Transbaikalian populations from the Siberian, showed the independence of the history of these regions. This corresponds more to the hypothesis that the modern area of Pinus sylvestris originated via settlement from many origins than to the hypothesized single center of the post-glacial recolonization. The distribution of the pairwise differences between the individual specimens corresponded to the model of sudden population growth. The assessments of the age of this event for Pinus sylvestris (4.5-4.7 million years), which were obtained on the basis of this model, significantly exceeded the age of the Quaternary. Therefore, the revealed population growth is hardly due to the changes in flora related with the glaciation, but rather mirrors the moment of the species formation.

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Держатели документа:
[Semerikov, V. L.
Semerikova, S. A.
Dymshakova, O. S.] Russian Acad Sci, Inst Plant & Anim Ecol, Ural Branch, Ekaterinburg 620144, Russia
[Zatsepina, K. G.
Tarakanov, V. V.
Tikhonova, I. V.
Ekart, A. K.] Russian Acad Sci, Sukachev Forest Inst, Siberian Branch, Krasnoyarsk 660036, Russia
[Vidyakin, A. I.] Russian Acad Sci, Inst Biol, Ural Branch, Komi Sci Ctr, Kirov 610035, Russia
[Jamiyansuren, S.] Acad Sci Mongolia, Inst Bot, Ulaanbaatar 210361, Mongol Peo Rep
[Rogovtsev, R. V.] Ctr Forest Protect Novosibirsk Reg, Novosibirsk 630015, Russia
[Kalchenko, L. I.] Ctr Forest Protect Altai Reg, Barnaul 656056, Russia
ИЛ СО РАН

Доп.точки доступа:
Semerikov, V.L.; Semerikova, S.A.; Dymshakova, O.S.; Zatsepina, K.G.; Tarakanov, V.V.; Tikhonova, I.V.; Ekart, A.K.; Vidyakin, A.I.; Jamiyansuren, S...; Rogovtsev, R.V.; Kalchenko, L.I.; Russian Foundation for Basic Researches [11-04-92226-Mong_a, 12-04-00062-a]; Program of the Ural Branch of the Russian Academy of Sciences [12-I-4-2064]

/ A. K. Ekart [et al.] // Russ. J. Gen. - 2016. - Vol. 52, Is. 3. - P273-280, DOI 10.1134/S1022795416030054 . - ISSN 1022-7954
Аннотация: The variability of 21 allozyme and three microsatellite loci of chloroplast DNA (cpDNA) was studied in the populations of Siberian spruce (Picea obovata Ledeb.) from Irkutsk oblast, Magadan oblast, Buryatia, and Mongolia. It was demonstrated that the highest level of genetic diversity among the examined populations at both allozyme and microsatellite loci was observed in the Tulyushka population from Irkutsk oblast. The lowest level of genetic diversity was observed in marginal isolated populations of Bogd Uul and Magadan. In the relict spruce population from Olkhon Island, differing from the other populations in the lowest allelic diversity of both types of markers, no expected decline of expected heterozygosity and haplotype diversity was observed. In this population, the variability parameters mentioned were close to the population mean. The obtained intrapopulation and intraspecific variability parameters of allozyme and microsatellite loci of chloroplast DNA and the data on the population differentiation at these loci indicate that the given markers can be used for the analysis of the population structure of Siberian spruce. © 2016, Pleiades Publishing, Inc.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Plant and Animal Ecology, Ural Branch, Russian Academy of Sciences, Yekaterinburg, Russian Federation

Доп.точки доступа:
Ekart, A. K.; Semerikova, S. A.; Semerikov, V. L.; Larionova, A. Y.; Kravchenko, A. N.; Dymshakova, O. S.

/ V. P. Vetrova [et al.] // Russ. J. Genet. Appl. Res. - 2016. - Vol. 6, Is. 5. - P560-568, DOI 10.1134/S2079059716050142 . - ISSN 2079-0597
Аннотация: The structures of Picea ajanensis populations were compared based on the allozyme analysis of vegetative buds and the morphometric analysis of generative organs. Six cenopopulations of P. ajanensis were investigated in areas with various levels of volcanic impact in the Kamchatka Peninsula. The genetic structures of spruce populations and phenotypes were determined by the analysis of ten enzyme systems (PGM, GOT, HK, LAP, MDH, SKDH, IDH, GDH, PGI, and SOD). The phenotypic variability of spruce populations was estimated based on the composition of morphotypes that were identified by using the geometric morphometrics of cone-scale shapes. Pairwise comparison of samples of cones from 170 trees from six populations revealed 12 morphotypes differing in the shape of the cone scales. Comparative assessment of the variability and similarity of the populations was carried out based on the frequency of the occurrence of the phenotypes and the frequency of the alleles of the polymorphic loci. Correlations of the genetic and phenotypic distance matrices between different phenotypes were revealed. This observation was consistent with the genetic determination of the shape of the cone scales in spruce. Genetic differences between the morphotypes with regard to nine polymorphic loci (Got-2, Skdh-1, Idh-2, Pgm-2, Mdh-1, Mdh-3, Pgm-1, Pgi-2, and Hk) were not significant. Statistically significant differences between the spruce morphotypes were revealed for two loci: Pgm-2 and Mdh-1. Differences in the genetic diversity of spruce populations generally corresponded to differences in their phenotypic diversity. The high levels of genetic and phenotypic diversity characterized a stable population structure of spruce in the area of weak volcanic influence. Changes in the genetic structure and low levels of the phenotypic diversity of spruce were observed under catastrophic volcanic impact. © 2016, Pleiades Publishing, Ltd.

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Держатели документа:
Kamchatka Branch, Pacific Geographical Institute, Far East Branch, Russian Academy of Sciences, Petropavlovsk-Kamchatsky, Russian Federation
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vetrova, V. P.; Ekart, A. K.; Kravchenko, A. N.; Larionova, A. Y.

/ N. V. Oreshkova [et al.] // Russ. J. Gen. - 2017. - Vol. 53, Is. 3. - P338-347, DOI 10.1134/S1022795417020089 . - ISSN 1022-7954
Аннотация: Variability of nuclear microsatellite loci was examined in Siberian dwarf pine. Six microsatellite loci (RPS2, RPS6, RPS12, RPS124, RPS127, Pc18) demonstrated different polymorphism levels in ten populations of Siberian dwarf pine. The average number of alleles per locus was 4.88, the average observed heterozygosity was 0.465, and the average expected heterozygosity was 0.510. About 13% of total genetic variability was explained by the genetic differences between the populations (FST = 0.129). Genetic distances between the examined populations of Pinus pumila inferred from the data on the SSR marker frequencies statistically significantly correlated with the geographical distances between the population samples. The level of genetic variability of the populations from Kamchatka Peninsula was lower than that demonstrated by continental and island populations. The genetic differentiation of the Kamchatka–Magadan and other populations of Siberian dwarf pine observed in our study can be explained in terms of their formation from different Pleistocene refugial centers. © 2017, Pleiades Publishing, Inc.

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Держатели документа:
Sukachev Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Kamchatka Branch of Pacific Geographical Institute, Far East Branch, Russian Academy of Sciences, Petropavlovsk-Kamchatsky, Russian Federation
Institute of Monitoring of Climatic and Ecological Systems, Siberian Branch, Russian Academy of Sciences, Tomsk, Russian Federation

Доп.точки доступа:
Oreshkova, N. V.; Vetrova, V. P.; Goroshkevich, S. N.; Petrova, E. A.

/ N. V. Oreshkova [et al.] // Russ. J. Genet. - 2017. - Vol. 53, Is. 11. - P1194-1199, DOI 10.1134/S1022795417110096. - Cited References:12. - The study was done as a part of the project "Genomics of the Key Boreal Forest Conifer Species and Then Major Phytopathogens in the Russian Federation" funded by the Government of the Russian Federation (grant no. 14.Y26.31.0004). . - ISSN 1022-7954. - ISSN 1608-3369РУБ Genetics & Heredity

Аннотация: This special issue of the journal is devoted to the outstanding population geneticist Yuri Petrovich Altukhov, who paid much attention in his research to the development of molecular genetic markers for population studies. Over the past time markers and methods of their development have undergone significant change. Thanks to modem methods of whole genome sequencing, it has become possible to develop markers of very different types-selectively neutral, as well as functional. Among them, microsatellite markers remain the most informative, convenient, reproducible, relatively inexpensive, and polymorphic. Whole genome sequencing greatly facilitates their discovery and development. This paper is devoted to the development of new microsatellite markers for a very important species of boreal forest-Siberian larch (Larix sibirica Ledeb.). Using a draft assembly of the larch genome, several thousand contigs containing microsatellite loci with di-, tri, tetra-, and pentanucleotide motifs were selected. A total of 59 pairs of PCR primers were tested for loci with dinucleotide motifs as the most variable. From them, 11 pairs were finally selected for 11 loci with dinucleotide repeats, which showed a high level of polymorphism and can be used in various population genetic studies and to identify the origin of wood and plant material.

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Держатели документа:
Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Georg August Univ Gottingen, D-37077 Gottingen, Germany.
Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 119333, Russia.
Texas A&M Univ, College Stn, TX 77843 USA.

Доп.точки доступа:
Oreshkova, N. V.; Putintseva, Yu. A.; Sharov, V. V.; Kuzmin, D. A.; Krutovsky, K. V.; Krutovsky, Konstantin; Government of the Russian Federation [14.Y26.31.0004]

/ M. A. Polezhaeva [et al.] // Russ. J. Genet. - 2019. - Vol. 55, Is. 6. - P762-766, DOI 10.1134/S1022795419060115. - Cited References:28. - This work (population collection) was carried out within the framework of the government order of the Research Institute of Ecology of Plants and Animals of the Ural Branch of the Russian Academy of Sciences. The molecular genetic analysis was supported by the Russian Foundation for Basic Research, grant no. 15-04-01640. . - ISSN 1022-7954. - ISSN 1608-3369РУБ Genetics & Heredity

Аннотация: SSR analysis of Rhododendron aureum Georgi in eight populations (63 samples) from Siberia (West and East Sayans) and Kamchatka was performed. Polymorphism of five SSR loci showed a high level of genetic diversity in all the populations (mean values H-o = 0.667, H-e = 0.638). The analysis of molecular variation (AMOVA) revealed that about 16% of the genetic diversity of R. aureum occurs in interpopulation differences (F-st = 0.161, p = 0.001). The Bayesian approach in the STRUCTURE program revealed that the species has a heterogeneous genetic structure-populations from Kamchatka form a separate cluster and show high homogeneity, and populations from Western and Eastern Siberia are of mixed origin. The obtained results indicate the prospects of using this type of markers for population-genetic studies of R. aureum.

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Держатели документа:
Russian Acad Sci, Inst Plant & Anim Ecol, Ural Branch, Ekaterinburg 620144, Russia.
Russian Acad Sci, Inst Biol Problems North, Far East Branch, Magadan 685000, Russia.
Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Polezhaeva, M. A.; Tikhonova, N. A.; Modorov, M., V; Ranyuk, M. N.; Korchagina, O. S.; Russian Foundation for Basic Research [15-04-01640]

[Текст] : статья / Л. И. Милютин, Т. Н. Новикова // Лесоведение. - 2019. - № 6. - С. 585-589 . - ISSN 0024-1148
   Перевод заглавия: Open Discussions in Forest Genetics and Breeding

УДК

630*165;

Аннотация: Целью работы является анализ дискуссионных проблем в современной лесной генетике и селекции. Рассматриваются вопросы взаимоотношений отбора лучших генотипов и популяций с одной стороны и сохранения уровня полиморфизма популяций, с другой. Анализируются и подвергаются сомнению выводы о доле внутрипопуляционной и межпопуляционной (географической) изменчивости в общей генетической изменчивости древесных растений. Обсуждается вопрос о внутривидовых таксонах, выделяемых в результате анализа географической изменчивости, в частности о генетической идентификации климатических экотипов (климатипов). Рассматривается проблема разработки лесосеменного районирования древесных растений на генетической основе.
The recent controversial topics in forest genetics and breeding were reported. Challenges of the selection of the best genotypes and populations and the preservation of polymorphism in populations were discussed. The current understanding of the contribution of intrapopulation and interpopulation (geographic) variability to genetic variability of trees was criticized. The question of intraspecific taxa, recognized on the basis of geographic variability, and, in particular, of the genetic identification of climatic ecotypes (climatypes), was discussed. The challenges of forest seed sources zonation of woody plants on a genetic basis were considered.

РИНЦ

Держатели документа:
Институт леса им. В.Н. Сукачева СО РАН

Доп.точки доступа:
Милютин, Леонид Иосифович; Milyutin, Leonid Iosifovich; Новикова, Татьяна Николаевна; Novikova, Tat'yana Nikolayevna

/ L. I. Mflyutin, T. N. Novikova // Russ. J. For. Sci. - 2019. - Vol. 2019, Is. 6. - С. 585-589, DOI 10.1134/S0024114819060068 . - ISSN 0024-1148
Аннотация: The recent controversial topics in forest genetics and breeding were reported. Challenges of the selection of the best genotypes and populations and the preservation of polymorphism in populations were discussed. The current understanding of the contribution of intrapopulation and interpopulation (geographic) variability to genetic variability of trees was criticized. The question of intraspecific taxa, recognized on the basis of geographic variability, and, in particular, of the genetic identification of climatic ecotypes (climatypes), was dis­cussed. The challenges of forest seed sources zonation of woody plants on a genetic basis were considered. © 2019, Russian Academy of Sciences. All rights reserved.

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Держатели документа:
Forest Institute, Siberian Branch of the Russian Academy of Sciences, Acaclemgorodok 50 bldg. 28, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Mflyutin, L. I.; Novikova, T. N.

[Текст] : статья / А. В. Пименов, А. Е. Коновалова, М. Е. Коновалова [и др.] // Лесоведение. - 2020. - № 6. - С. 493-502 . - ISSN 0024-1148

УДК

574.38

674.032.475.442

Аннотация: Впервые проанализированы связи соотношения числа деревьев желто- и краснопыльниковой форм сосны обыкновенной (Pinus sylvestris L.) и экотопических условий с использованием взаимной энтропии подсистем и частной информации. Установлено, что структура популяций сосны обыкновенной имеет статистически значимую связь с характеристиками экотопа. Долевое участие деревьев краснопыльниковой формы увеличивается в пессимальных для вида условиях произрастания. Исследования проведены в двух географо-климатических зонах юга Сибири.
For the first time the correlation of the number of yellow and red anther forms of Scots pine (Pinus sylvestris L.) and the ecotope conditions was studied using the relative entropy of subsystems and self-information. It was determined that the structure of the pine populations has a statistically relevant correlation with the ecotope characteristics. The fraction of the red anther form of pine increases in the pessimal growth conditions. The studies were conducted within the two geographical climatic zones of the South of Siberia.

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Держатели документа:
Институт леса им. В.Н. Сукачева СО РАН – обособленное подразделение ФИЦ КНЦ СО РАН

Доп.точки доступа:
Пименов, Александр Владимирович; Коновалова, А.Е.; Коновалова, Мария Евгеньевна; Кофман, Григорий Борисович; Седельникова, Тамара Станиславовна; Ефремов, Станислав Петрович

/ N. V. Oreshkova, T. S. Sedel’nikova, S. P. Efremov, A. V. Pimenov // Contemp. Probl. Ecol. - 2020. - Vol. 13, Is. 6. - P569-576, DOI 10.1134/S1995425520060116 . - ISSN 1995-4255
Аннотация: Abstract: The DNA polymorphism of seven coenopopulations of Siberian stone pine (Pinus sibirica Du Tour) growing in a region which is typical for mountain taiga pine forests—the northeastern part of the Kuznetsk Alatau—has been studied. According to the data of 11 nuclear microsatellite loci, 44 allelic variants which significantly differed between the studied coenopopulations in composition and frequency of occurrence are identified. The highest level of allelic diversity is found in the Ps_80612 and Ps_1502048 loci, in which eight and seven alleles are detected, respectively. The calculation of the main parameters of genetic diversity have shown, on the whole, a relatively low level of polymorphism of the studied samples (NA = 3.078, NE = 1.877, HE = 0.445, and HO = 0.401). An analysis of the degree of subdivision of coenopopulations has revealed that about 95% of the total genetic diversity is concentrated within populations, while the interpopulation component accounts for only about 5% (FST = 0.049). The diagnosed differences in the level of P. sibirica genetic polymorphism are determined by several factors: the presence of orographic and phytocenotic barriers between the coenopopulations, which define the directions of seed propagation vectors along the watercourses of river basins that form the Chulym River in the eastern sector of the Kuznetsk Alatau and Tom River in the western sector; a significant level of bioclimatic and environmental extremeness of habitats of coenopopulations in accordance with the height above sea level; and anthropogenic pressure, including periodic deforestation and recreational impact on coenopopulations located near settlements. These factors, which have a significant microevolutionary component, determine the adaptation trends of coenopopulations of P. sibirica and morphological singularities and reproductive potential of trees and, ultimately, they control their genetic polymorphism in the studied part of the Kuznetsk Alatau. © 2020, Pleiades Publishing, Ltd.

Scopus

Держатели документа:
Federal Research Center, Kola Scientific Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Sukachev Institute of Forestry, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Oreshkova, N. V.; Sedel’nikova, T. S.; Efremov, S. P.; Pimenov, A. V.

/ N. V. Oreshkova, T. S. Sedel'nikova, S. P. Efremov, A. V. Pimenov // Contemp. Probl. Ecol. - 2020. - Vol. 13, Is. 6. - P569-576, DOI 10.1134/S1995425520060116. - Cited References:28 . - ISSN 1995-4255. - ISSN 1995-4263РУБ Ecology

Аннотация: The DNA polymorphism of seven coenopopulations of Siberian stone pine (Pinus sibirica Du Tour) growing in a region which is typical for mountain taiga pine forests-the northeastern part of the Kuznetsk Alatau-has been studied. According to the data of 11 nuclear microsatellite loci, 44 allelic variants which significantly differed between the studied coenopopulations in composition and frequency of occurrence are identified. The highest level of allelic diversity is found in the Ps_80612 and Ps_1502048 loci, in which eight and seven alleles are detected, respectively. The calculation of the main parameters of genetic diversity have shown, on the whole, a relatively low level of polymorphism of the studied samples (N-A = 3.078, N-E = 1.877, H-E = 0.445, and H-O = 0.401). An analysis of the degree of subdivision of coenopopulations has revealed that about 95% of the total genetic diversity is concentrated within populations, while the interpopulation component accounts for only about 5% (F-ST = 0.049). The diagnosed differences in the level of P. sibirica genetic polymorphism are determined by several factors: the presence of orographic and phytocenotic barriers between the coenopopulations, which define the directions of seed propagation vectors along the watercourses of river basins that form the Chulym River in the eastern sector of the Kuznetsk Alatau and Tom River in the western sector; a significant level of bioclimatic and environmental extremeness of habitats of coenopopulations in accordance with the height above sea level; and anthropogenic pressure, including periodic deforestation and recreational impact on coenopopulations located near settlements. These factors, which have a significant microevolutionary component, determine the adaptation trends of coenopopulations of P. sibirica and morphological singularities and reproductive potential of trees and, ultimately, they control their genetic polymorphism in the studied part of the Kuznetsk Alatau.

WOS

Держатели документа:
Russian Acad Sci, Fed Res Ctr, Kola Sci Ctr, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Oreshkova, N., V; Sedel'nikova, T. S.; Efremov, S. P.; Pimenov, A., V

[Текст] : статья / Наталья Викторовна Орешкова, Тамара Станиславовна Седельникова, Станислав Петрович Ефремов, Александр Владимирович Пименов // Сибирский экологический журнал. - 2020. - Т. 27, № 6. - С. 677-688 . - ISSN 0869-8619

УДК

575.174.015.3

582.475.2

Аннотация: Исследован полиморфизм ДНК семи ценопопуляций сосны сибирской кедровой (Pinus sibirica Du Tour), произрастающих в характерном для горно-таежных кедровников регионе - северо-восточной части Кузнецкого Алатау. По 11 ядерным микросателлитным локусам выявлено 44 аллельных варианта, существенно отличающихся у изученных ценопопуляций по составу и частотам встречаемости. Самый высокий уровень аллельного разнообразия имеют локусы Ps _80612, Ps _1502048, в которых выявлено 8 и 7 аллелей соответственно. Расчет основных параметров генетического разнообразия показал в целом сравнительно невысокий уровень полиморфизма исследованных выборок ( NA = 3,078; NE = 1,877; HE = 0,445; HO = 0,401). Анализ степени подразделенности ценопопуляций выявил, что внутри популяций сосредоточено около 95 % всего генетического разнообразия, тогда как на межпопуляционную составляющую приходится всего около 5 % (F ST = 0,049). Диагностированные различия в уровне генетического полиморфизма P. sibirica определяются: наличием между ценопопуляциями орографических и фитоценотических барьеров, обусловливающих направления векторов распространения семян вдоль водотоков бассейнов рек, формирующих р. Чулым в восточном секторе Кузнецкого Алатау и р. Томь - в западном; значительным уровнем биоклиматической и экологической экстремальности местопроизрастаний ценопопуляций в соответствии с высотой над уровнем моря; антропогенной нагрузкой, включающей периодические рубки и рекреационное воздействие на расположенные вблизи населенных пунктов ценопопуляции. Данные факторы, имеющие выраженную микроэволюционную составляющую, детерминируют адаптивные тренды ценопопуляций P. sibirica, морфологическое своеобразие и репродуктивный потенциал деревьев и, в конечном счете, контролируют их генетический полиморфизм в исследованной части Кузнецкого Алатау.
The DNA polymorphism of 7 coenopopulations of Siberian stone pine ( Pinus sibirica Du Tour), growing in the region which is typical for mountain taiga pine forests - the north-eastern part of the Kuznetsk Alatau, was studied. According to the data of 11 nuclear microsatellite loci, 44 allelic variants, which significantly differed between the studied cenopopulations in composition and frequency of occurrence were identified. The highest level of allelic diversity is found in the Ps_80612, Ps_1502048 loci, in which 8 and 7 alleles were detected, respectively. The calculation of the main parameters of genetic diversity showed, on the whole, a relatively low level of polymorphism of the studied samples ( NA = 3,078; NE = 1,877; HE = 0,445; HO = 0,401). An analysis of the degree of subdivision of cenopopulations revealed that about 95 % of the total genetic diversity is concentrated within populations, while the interpopulation component accounts for only about 5 % ( FST = 0,049). The diagnosed differences in the level of P. sibirica genetic polymorphism are determined by several factors: the presence of orographic and phytocenotic barriers between the coenopopulations which defined the directions of seed propagation vectors along the watercourses of river basins that formed the Chulym river in the eastern sector of the Kuznetsk Alatau and Tom river in the western sector; a significant level of bioclimatic and environmental extremeness of habitats of coenopopulations in accordance with the height above sea level; anthropogenic pressure, including periodic deforestation and recreational impact on coenopopulations located near settlements. These factors, which have a significant microevolutionary component, determine the adaptation trends of cenopopulations of P. sibirica , morphological singularities and reproductive potential of trees and, ultimately, control their genetic polymorphism in the studied part of the Kuznetsk Alatau

РИНЦ

Держатели документа:
Институт леса им. В. Н. Сукачева СО РАН
Сибирский федеральный университет
ФИЦ КНЦ СО РАН

Доп.точки доступа:
Орешкова, Наталья Викторовна; Седельникова, Тамара Станиславовна; Ефремов, Станислав Петрович; Пименов, Александр Владимирович

/ И. В. Тихонова, А. К. Экарт, А. Н. Кравченко, Н. А. Тихонова // Генетика. - 2021. - Т. 57, № 3. - С. 296-310, DOI 10.31857/S0016675821030139 . - ISSN 0016-6758

УДК	630*46:575.174.015.3:582.47

Аннотация: Исследована изменчивость полиморфных ферментных локусов у 1180 особей подроста в четырех популяциях Pinus sylvestris L., Picea obovata Ledeb. и Abies sibirica Ledeb., произрастающих в условиях южной тайги Средней Сибири, на участках, пройденных рубками разной интенсивности, и в контроле (в том числе, в 15 выборках сосны обыкновенной, 13 выборках ели сибирской и 16 выборках пихты сибирской). Результаты сравнительного анализа данных подтверждают существенное сокращение генетического разнообразия у молодого поколения деревьев трех хвойных видов на месте сплошных широколесосечных и выборочных вырубок высокой интенсивности (на 8–30% – по числу аллелей и уровню полиморфности, на 14–75% – по числу редких аллелей). Отмечено, что генофонды популяций темнохвойных видов более чувствительны к антропогенным воздействиям по сравнению со светлохвойным (сосной обыкновенной). Особенности генетической изменчивости изоферментов в популяциях пихты сибирской свидетельствуют о значительной утрате видом внутривидового аллельного разнообразия ферментных локусов, что требует особого подхода к анализу влияния лесопользования на популяции данного вида и сохранению его генетического разнообразия. Из использованных показателей генетической изменчивости наиболее информативны для целей генетического мониторинга хвойных лесов редкие аллели полиморфных локусов в целом и особенно аллели низкополиморфных локусов
We studied the variability of polymorphic isozyme loci in 1180 individuals of undergrowth in 4 populations of Scots pine (Pinus sylvestris L.), Siberian spruce (Picea obovata Ledeb.) and Siberian fir (Abies sibirica Ledeb.), growing in the southern taiga of Central Siberia, on the sites passed by logging of different intensities and in the control. In total, 15 samples of Scots pine, 13 samples of Siberian spruce, and 16 samples of Siberian fir were studied within the populations. A comparative analysis of the data confirms a significant reduction in genetic diversity of young generation of trees of three coniferous species (by 8-30% in the number of alleles and the level of polymorphism of loci, by 14-75% in the number of rare alleles) in the place of clear large-scale cuttings and selective cuttings of high intensity. It was noted that the gene pools of populations of dark coniferous species are more sensitive to anthropogenic impacts compared with light coniferous (Scots pine). Features of the genetic variation of allozymes in Siberian fir populations indicate a significant loss by the species of the intraspecific allelic diversity of the enzyme loci, therefore it is necessary to use another markers and approach to the analysis of the impact of forest management on the population of this species as well as greater care for the conservation of its genetic diversity. Of the used indicators of genetic variation the most informative for the genetic monitoring of coniferous forests are rare alleles of polymorphic loci in general, and especially rare alleles of loci characterized by a low level of polymorphism. Of the used indicators of genetic variability, the most informative for the purposes of genetic monitoring of coniferous forests are rare alleles of polymorphic loci in general, and especially alleles of low polymorphic loci

Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Тихонова, Ирина Васильевна; Tikhonova Irina Vasil'yevna; Экарт, Александр Карлович; Ekart, Alexandr Karlovich; Кравченко, Анна Николаевна; Kravchenko, Anna Nikolayevna; Тихонова, Наталья Александровна; Tikhonova Natal'ya Alexandrovna