/ Y. A. Putintseva, E. I. Bondar, E. P. Simonov [et al.] // BMC Genomics. - 2020. -
Vol. 21,
Is. 1. - P654,
DOI 10.1186/s12864-020-07061-4
. - ISSN 1471-2164
Аннотация: BACKGROUND: Plant mitochondrial genomes (mitogenomes) can be structurally complex while their size can vary from ~?222 Kbp in Brassica napus to 11.3 Mbp in Silene conica. To date, in comparison with the number of plant species, only a few plant mitogenomes have been sequenced and released, particularly for conifers (the Pinaceae family). Conifers cover an ancient group of land plants that includes about 600 species, and which are of great ecological and economical value. Among them, Siberian larch (Larix sibirica Ledeb.) represents one of the keystone species in Siberian boreal forests. Yet, despite its importance for evolutionary and population studies, the mitogenome of Siberian larch has not yet been assembled and studied. RESULTS: Two sources of DNA sequences were used to
search for mitochondrial DNA (mtDNA) sequences: mtDNA enriched samples and nucleotide reads generated in the de novo whole genome sequencing project, respectively. The assembly of the Siberian larch mitogenome contained nine contigs, with the shortest and the largest contigs being 24,767?bp and 4,008,762?bp, respectively. The total size of the genome was estimated at 11.7 Mbp. In total, 40 protein-coding, 34 tRNA, and 3 rRNA genes and numerous repetitive elements (REs) were annotated in this mitogenome. In total, 864 C-to-U RNA editing sites were found for 38 out of 40 protein-coding genes. The immense size of this genome, currently the largest reported, can be partly explained by variable numbers of mobile genetic elements, and introns, but unlikely by plasmid-related sequences. We found few plasmid-like insertions representing only 0.11% of the entire Siberian larch mitogenome. CONCLUSIONS: Our study showed that the size of the Siberian larch mitogenome is much larger than in other so far studied Gymnosperms, and in the same range as for the annual flowering plant Silene conica (11.3 Mbp). Similar to other species, the Siberian larch mitogenome contains relatively few genes, and despite its huge size, the repeated and low complexity regions cover only 14.46% of the mitogenome sequence.
Scopus Держатели документа: Laboratory of Forest Genomics, Genome Research and Education Center, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, 660036, Russian Federation
Laboratory of Genomic Research and Biotechnology, Federal Research Center "Krasnoyarsk Science Center", Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation
Institute of Environmental and Agricultural Biology (X-BIO), University of Tyumen, Tyumen, 625003, Russian Federation
Department of High Performance Computing, Institute of Space and Information Technologies, Siberian Federal University, Krasnoyarsk, 660074, Russian Federation
Laboratory of Forest Genetics and Selection, V. N. Sukachev Institute of Forest, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation
Laboratory of Plant Genetic Engineering, Siberian Institute of Plant Physiology and Biochemistry, Russian Academy of Sciences, Siberian Branch, Irkutsk, 664033, Russian Federation
Institute of Computational Modeling, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation
Department of Plant Physiology, UPSC, Umea University, Umea, S-90187, Sweden
Department of Forest Genetics and Forest Tree Breeding, Georg-August University of Gottingen, Gottingen37077, Germany
Center for Integrated Breeding Research, George-August University of Gottingen, Gottingen37075, Germany
Laboratory of Population Genetics, N.I. Vavilov Institute of General Genetics, Russian Academy of SciencesMoscow 119333, Russian Federation
Department of Ecosystem Science and Management, Texas A&M University, TX, College Station, United States
Доп.точки доступа: Putintseva, Y. A.; Bondar, E. I.; Simonov, E. P.; Sharov, V. V.; Oreshkova, N. V.; Kuzmin, D. A.; Konstantinov, Y. M.; Shmakov, V. N.; Belkov, V. I.; Sadovsky, M. G.; Keech, O.; Krutovsky, K. V.
