Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / I. V. Shein [et al.] // Russ. J. Plant Physiol. - 2003. - Vol. 50, Is. 5. - P634-639, DOI 10.1023/A:1025688023862. - Cited References: 27 . - 6. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Pine (Pinus sylvestris L.) callus culture was treated with the mycelium extracts from six Fusarium strains. Previously, pine seedlings were infected with a spore suspension in order to test the pathogenicity of the used strains. Callus culture infection resulted in a decrease in the free proanthocyanidin (PA) and an increase in bound PA content. After treating the calli with all strains except F. oxysporum var. orthoceras, the lignin content became lower than the control one. The most considerable changes involved the p-hydroxybenzoic acid (HBA) content, and its greatest change was observed after treating the calli with F. nivale, when the HBA concentration (1229 mug/g, dry wt) exceeded fourfold the control one. There was a positive correlation (R = 0.81) between the HBA content in the callus culture cells treated with a fungal extract and the virulence of Fusarium strains. At the same time, there was an inverse correlation (R = -0.80) between the lignin content in a callus culture and the fungal virulence; the latter did not affect the contents of both free and bound PA.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia
Ctr Forest Def Krasnoyarsk Krai, Krasnoyarsk, Russia

Доп.точки доступа:
Shein, I.V.; Andreeva, O.N.; Polyakova, G.G.; Zrazhevskaya, G.K.

/ I. N. Pavlov [et al.] // Contemp. Probl. Ecol. - 2018. - Vol. 11, Is. 4. - P406-419, DOI 10.1134/S1995425518040054. - Cited References:27. - This study was supported by the Russian Foundation for Basic Research, the Government of Krasnoyarsk krai, and the Krasnoyarsk Regional Fund for Support of Scientific and Technical Activity, project no. 16-44-242145r_ofi_m. . - ISSN 1995-4255. - ISSN 1995-4263РУБ Ecology

Аннотация: The territory of the new mass outbreak of Dendrolimus sibiricius is northernmost Siberia. The present increase in the pest numbers started in 2011-2012, 14 years after the previous mass outbreak. As a result, the area of damage to dark coniferous forests exceeded 1.4 million ha. The reasons for the emergence of the pest population from a depressive state are insufficient moisture availability in May 2011, severe droughts in June-July 2012, and increased mean monthly temperature. A significant decrease in the vegetation index of SWVI occurred in 2011-2012. The hydrothermal regime of the spring-summer period in these years promoted the transition of most of the pest populations to development over 1-year generation. One exceptional feature of the current outbreak is low damage to D. sibiricus caterpillars by diseases and parasites. Favorable weather conditions and a low number of natural entomopathogens contribute to the rapid spread of the pest in northern and eastern directions. This creates a high risk of mass outbreak of D. sibiricus in dark coniferous forests, since the area of nearby dark coniferous stands suitable in structure, climatic, and forest-growing conditions for the pest dispersal is more than 2.7 million ha. Pure cultures of the entomopathogenic fungus Beauveria bassiana were isolated from dead caterpillars of D. sibiricus. Multistage screening made it possible to select a promising strain for the creation of a long-acting bioinsecticide for the preventive treatment of forests. The main selection criteria are high virulence, high entomopathogenic activity at low temperatures (7-10A degrees C), and the ability for solid-phase fermentation of plant waste.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660049, Russia.
Fed Forestry Agcy Russian Ctr Forest Hlth, Ctr Forest Hlth Krasnoyarsk Krai, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Pavlov, I. N.; Litovka, Y. A.; Golubev, D. V.; Astapenko, S. A.; Chromogin, P. V.; Russian Foundation for Basic Research; Government of Krasnoyarsk krai; Krasnoyarsk Regional Fund for Support of Scientific and Technical Activity [16-44-242145r_ofi_m]

/ I. N. Pavlov, Y. A. Litovka, D. V. Golubev [et al.] // Contemp. Probl. Ecol. - 2020. - Vol. 13, Is. 1. - P71-84, DOI 10.1134/S1995425520010060. - Cited References:59 . - ISSN 1995-4255. - ISSN 1995-4263РУБ Ecology

Аннотация: Conifer decline (Abies sibirica Ledeb. stands) as a result of complex biotic effects on the territory of Krasnoyarsk krai by 2018 reached an area of 541 400 ha; the potential area of further death of A. sibirica is up to 10 million ha. The main reason is the phytopathogenic fungi Armillaria mellea s.l. and Heterobasidion annosum s.l. combined with bark beetle Polygraphus proximus. Trees affected by root pathogens are centers of forest dieback, from which pathological outbreaks spread, including the effects of P. proximus and its phytopathogenic mycoassociates. Neonectria fuckeliana (C. Booth) Castl & Rossman is among the concomitant pathogens in the scale of the lesion, which causes a resin flow that is similar to the effects of P. proximus attack. The phytopathogenicity of pure cultures of the dominant root pathogens of Armillaria borealis Marxm. & Korhonen and Heterobasidion annosum (Fr.) Bref., as well as the stem pathogen of N. fuckeliana, has been confirmed by a series of experiments in vitro. Promising natural agents for the regulation of P. proximus abundance are highly virulent aboriginal strains of Beauveria bassiana (Bals.-Criv.) Vuill., resulting in maximum insect death (up to 100%) for 7-11 days and retaining activity in the temperature range of 16-24 +/- 1 degrees C.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660037, Russia.
Federal Forestry Agcy, Russian Ctr Forest Hlth Branch, Ctr Forest Hlth Krasnoyarsk Krai, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Pavlov, I. N.; Litovka, Y. A.; Golubev, D. V.; Astapenko, S. A.; Chromogin, P. V.; Usoltseva, Y. V.; Makolova, P. V.; Petrenko, S. M.

/ И. Д. Гродницкая, В. А. Сенашова, М. Ю. Трусова, О. Э. Пашкеева, Ю. Н. Баранчиков // Сибирский лесной журнал. - 2023. - № 1. - P70-84, DOI 10.15372/SJFS20230107 . - ISSN 2311-1410
Аннотация: В настоящее время в темнохвойных лесах Прибайкалья распространено заболевание бактериальная водянка (bacterial wetwood), вызывающее ухудшение санитарного состояния древостоев и приводящее к гибели значительных лесных массивов. Несмотря на то что оно известно еще с начала прошлого века, вопрос о его истинном возбудителе до сих пор остается открытым. Полагают, что в развитии патогенеза могут принимать участие ассоциации различных эндофитных микроорганизмов. В связи с этим представляется актуальным исследование состава эндофитного комплекса микроорганизмов древесины больных водянкой лесных пород для установления типов взаимоотношений между эндофитами и проверки их способности инициациировать патологический процесс у хвойных. Сделана попытка выявить основных представителей эндофитного микробиома «мокрой древесины» сосны кедровой сибирской ( Pinus sibirica Du Tour), пораженной бактериальной водянкой, оценить их фитопатогенные свойства и типы взаимоотношений между ними. Исследования проводились в Слюдянском лесничестве Иркутской области. В работе использованы стандартные микробиологические и фитопатологические методы для выделения чистых культур бактерий и изучения их особенностей (в том числе вирулентности), а также молекулярно-генетические методы для идентификации бактерий. Из «мокрой древесины» пораженных бактериальной водянкой деревьев сосны кедровой сибирской впервые выделен комплекс чистых культур эндофитных микроорганизмов. Среди них к условно-патогенным штаммам относятся Rouxiella chamberiensis и Ewingella americana , Stenotrophomonas rhizophila и Bacillus pumilis, проявляющие выраженные вирулентные свойства по отношению к индикаторным растениям и неоднозначно влияющие на рост и развитие сеянцев сосны обыкновенной ( Pinus sylvestris L.). В эндофитном микробиоме пораженной древесины кедра кроме взаимодействия с растением отмечено и взаимное влияние бактерий. Выраженные антагонистические свойства проявляли бактерии р. Bacillus. В основном в эндофитном сообществе преобладали взаимоотношения симбиотического характера, с возможными синтрофными связями
Currently, in the dark coniferous forests of the Baikal region, the spread of the disease bacterial wetwood disease is noted, causing the sanitary condition deterioration in the forest stands, and leading to the forest dieback on large territories. Despite the fact that this disease has been known since the beginning of the last century, the question of its’ real pathogen is still open. It is believed that associations of various endophytic microorganisms may take part in the development of pathogenesis. In this regard, it seems relevant to study the composition of the endophytic complex of microorganisms in wood with bacterial wetwood of forest species in order to establish the types of relationships between endophytes and test their ability to initiate a pathological process in conifers. An attempt was made to identify the main endophytic microbiome representatives of the «wet wood» of Siberian stone pine ( Pinus sibirica Du Tour) affected by bacterial wetwood, to assess their phytopathogenic properties and types of relationships between them. The features of the endophytic microbiome of the Siberian stone pine affected by bacterial wetwood in the Slyudyanskoye forestry of the Irkutsk region were studied. With standard microbiological and phytopathological methods we isolated pure cultures of bacteria and studied their characteristics (including virulence). For bacterias identification molecular genetic methods were used. From the infested wood of Siberian stone pine trees affected by bacterial wetwood, a complex of pure microorganisms cultures, not previously described in other studies, was isolated. Among them, opportunistic strains include Rouxiella chamberiensis, Ewingella americana, Stenotrophomonas rhizophila and Bacillus pumilus , which exhibit pronounced virulent properties in relation to indicator plants and ambiguously affect the growth and development of Scotch pine ( Pinus sylvestris L.) seedlings. Mutual impacts of bacteria on each other were noted in the endophytic microbiome of the affected Siberian stone pine wood. Bacillus bacteria showed pronounced antagonistic properties. Basically, in the endophytic community symbiotic relationships were dominated with possible syntrophic connections

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Держатели документа:
ИЛ СО РАН : 660036, Красноярск, Академгородок, 50, стр. 28

Доп.точки доступа:
Сенашова, Вера Александровна; Syenashova Vera Alexandrovna; Трусова, Мария Юрьевна; Trusova M.Yu.; Пашкеева, Оксана Эриковна; Баранчиков, Юрий Николаевич; Baranchikov, Yury Nikolayevich; Grodnitskaya, Irina Dmitriyevna

/ V. S. Akulova, V. V. Sharov, A. I. Aksyonova [et al.] // BMC Genomics. - 2020. - Vol. 21. - Ст. 534, DOI 10.1186/s12864-020-06964-6. - Cited References:48. - This work including the study and collection, analysis and interpretation of data, and writing the manuscript was supported by research grant. 14.Y26.31.0004 from the Government of the Russian Federation with partial funding from the Federal Research Center "Krasnoyarsk Scientific Center", Siberian Branch, Russian Academy of Sciences (grants No 0287-2019-0002, No 0356-2016-0704, and No 0356-2019-0024). The funding agencies played no role in the design of the study and collection material, analysis and interpretation of data, and in writing the manuscript. Publication cost have been funded by the Open Access Publication Funds of the University of Gottingen. . - ISSN 1471-2164РУБ Biotechnology & Applied Microbiology + Genetics & Heredity

Аннотация: Background: Massive forest decline has been observed almost everywhere as a result of negative anthropogenic and climatic effects, which can interact with pests, fungi and other phytopathogens and aggravate their effects. Climatic changes can weaken trees and make fungi, such as Armillaria more destructive. Armillaria borealis (Marxm. & Korhonen) is a fungus from the Physalacriaceae family (Basidiomycota) widely distributed in Eurasia, including Siberia and the Far East. Species from this genus cause the root white rot disease that weakens and often kills woody plants. However, little is known about ecological behavior and genetics of A. borealis. According to field research data, A. borealis is less pathogenic than A. ostoyae, and its aggressive behavior is quite rare. Mainly A. borealis behaves as a secondary pathogen killing trees already weakened by other factors. However, changing environment might cause unpredictable effects in fungus behavior. ResultsThe de novo genome assembly and annotation were performed for the A. borealis species for the first time and presented in this study. The A. borealis genome assembly contained similar to 68 Mbp and was comparable with similar to 60 and similar to 79.5 Mbp for the A. ostoyae and A. mellea genomes, respectively. The N50 for contigs equaled 50,544bp. Functional annotation analysis revealed 21,969 protein coding genes and provided data for further comparative analysis. Repetitive sequences were also identified. The main focus for further study and comparative analysis will be on the enzymes and regulatory factors associated with pathogenicity. ConclusionsPathogenic fungi such as Armillaria are currently one of the main problems in forest conservation. A comprehensive study of these species and their pathogenicity is of great importance and needs good genomic resources. The assembled genome of A. borealis presented in this study is of sufficiently good quality for further detailed comparative study on the composition of enzymes in other Armillaria species. There is also a fundamental problem with the identification and classification of species of the Armillaria genus, where the study of repetitive sequences in the genomes of basidiomycetes and their comparative analysis will help us identify more accurately taxonomy of these species and reveal their evolutionary relationships.

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Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Lab Forest Genom, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Krasnoyarsk Sci Ctr, Siberian Branch, Lab Genom Res & Biotechnol,Fed Res Ctr, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Inst Space & Informat Technol, Dept High Performance Comp, Krasnoyarsk 660074, Russia.
Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forest, Lab Forest Genet & Select, Krasnoyarsk 660036, Russia.
Natl Res Tech Univ, Dept Informat, Irkutsk 664074, Russia.
Russian Acad Sci, Siberian Branch, Limnol Inst, Irkutsk 664033, Russia.
Russian Acad Sci, Siberian Branch, VN Sukachev Inst Forest, Lab Reforestat Mycol & Plant Pathol, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Dept Chem Technol Wood & Biotechnol, Krasnoyarsk 660049, Russia.
Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, D-37077 Gottingen, Germany.
George August Univ Gottingen, Ctr Integrated Breeding Res, D-37075 Gottingen, Germany.
Russian Acad Sci, NI Vavilov Inst Gen Genet, Lab Populat Genet, Moscow 119333, Russia.
Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA.

Доп.точки доступа:
Akulova, Vasilina S.; Sharov, Vadim V.; Aksyonova, Anastasiya I.; Putintseva, Yuliya A.; Oreshkova, Natalya V.; Feranchuk, Sergey I.; Kuzmin, Dmitry A.; Pavlov, Igor N.; Litovka, Yulia A.; Krutovsky, Konstantin V.; Krutovsky, Konstantin; Government of the Russian Federation [14.Y26.31.0004]; Federal Research Center "Krasnoyarsk Scientific Center", Siberian Branch, Russian Academy of Sciences [0287-2019-0002, 0356-2016-0704, 0356-2019-0024]; University of Gottingen

/ T. V. Antipova, V. P. Zhelifonova, Y. A. Litovka [et al.] // iForest. - 2022. - Vol. 15. - P38-46, DOI 10.103832/ifor3840014. - Cited References:28 . - ISSN 1971-7458РУБ Forestry

Аннотация: Basidiomycetes Armillaria infect deciduous, coniferous and fruit trees, causing enormous economic damage. The role of secondary metabolites (tricyclic sesquiterpene aryl esters - melleolides) in the life cycle and pathogenesis of Armillaria is under active investigation. To date, not all species of Armillaria have been tested for the biosynthesis of melleolides. We investigated the secondary metabolite profiles of six root-pathogenic species of the genus Armillaria (A. borealis Marxmuller & Korhonen, A. cepistipes Velenovsky, A. gallica Marxm, A. mellea (Vahl) P. Kummer, A. sinapina Berube & Dessur, A. ostoyae (Romagn.) Herink) distributed in Siberia (South Krasnoyarsk Krai, Republic of Tyva, Republic of Khakassia, Taimyr Peninsula), Russian Far East (Sikhote-Alin) and Crimea (Krymsky National Park, Chatyr-Dag Mountain Lower Plateau). A total of 15 compounds were identified in the metabolome profile. Two compounds (melleolide D and melledonal C) are synthesized by all investigated strains irrespective of their geographic location and host plant. The maximum spectrum of melleolides (7-8 compounds) was found in isolates of A. borealis, A. gallica, A. sinapina, A. ostoyae. In submerged culture, the maximum accumulation of melleolides varied from 2 up to 239 mg l(-1). A mixture of melleolide D and melledonal C (1:1) synthesized by the most productive strain A. mellea Cr2-17 was first found to have a phytotoxic action on the growth parameters of the callus culture Populus balsamifera and 10-day-old conifer seedlings. A 0.5% concentration of melleolides caused a credible decrease of P. balsamifera callus raw biomass; a decrease of the viability of Larix sibirica and, which is especially significant, Pinus sylvestris seedlings; inhibition of stem and root growth processes; dechromation of foliage; loss of turgor. The occurrence of a broad range of melleolides in the metabolome profile and two common compounds in all investigated strains, with a phytotoxic action at their sufficiently high concentration, enables considering the synthesis of melleolides by Armillaria fungi as one of the possible mechanisms of their pathogenicity efficiently realized in strains characterized by overproduction of melleolides under natural conditions.

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Держатели документа:
Russian Acad Sci, GK Skryabin Inst Biochem & Physiol Microorganisms, FRC Pushchino Ctr Biol Res, 5 Prosp Nauki, Pushchino 142290, Moscow Region, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, FRC KSC, Siberian Branch, 50 Akad Gorodok Str, Krasnoyarsk 660036, Russia.
FSBEIHE MF Reshetnev Siberian State Univ Sci & Te, 82 Prosp Mira, Krasnoyarsk 660037, Russia.
FSBEIHE Krasnoyarsk State Agr Univ, 90 Prosp Mira, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Antipova, Tatyana, V; Zhelifonova, Valentina P.; Litovka, Yulia A.; Pavlov, Igor N.; Baskunov, Boris P.; Kokh, Zhanna A.; Makolova, Polina, V; Timofeev, Anton A.; Kozlovsky, Anatoly G.