Рубрики:
N-15 NATURAL-ABUNDANCE
N-2 FIXATION
ACETYLENE-REDUCTION
ALTITUDINAL GRADIENT
SUBTERRANEAN CLOVER
BIOMASS PRODUCTION
PASTURE LEGUMES
WHITE CLOVER
TUNDRA
SOIL
N-15 NATURAL-ABUNDANCE
N-2 FIXATION
ACETYLENE-REDUCTION
ALTITUDINAL GRADIENT
SUBTERRANEAN CLOVER
BIOMASS PRODUCTION
PASTURE LEGUMES
WHITE CLOVER
TUNDRA
SOIL
Аннотация: The symbiotic fixation of atmospheric nitrogen by leguminous plants in the alpine community of a lichen heath at the Teberda State Biosphere Reserve is well adapted to low soil temperature characteristic for the altitude of 2800 m a.s.l. For the determination of the N fixation by isotopic methods (the method of the natural (15)N abundance and the method of isotopic (15)N dilution), Trifolium polyphyllum was taken as the control plant. This plant was used as it does not form symbiosis with the nitrogen-fixing bacteria in the highlands of the Northern Caucasus Region. The contribution of the N fixation to the N nutrition of different leguminous plant species as determined by the natural (15)N abundance method amounted to 28-73% at delta(15)N(0) = 0aEuro degrees and 46-117% at delta(15)N(0) = -1aEuro degrees; for the determination of the N fixation by the method of the isotopic label's dilution, it was 34-97%. The best correlation of the results obtained by these two isotopic methods was observed for the natural fractionation of the N isotopes in the course of the N fixation in the range of -0.5 to -0.7aEuro degrees. The determination of the nitrogenase activity of the roots by the acetylene method confirmed the absence of N fixation in T. polyphyllum and its different contribution to the N nutrition of different species of leguminous plants.
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Держатели документа:
[Makarov, M. I.
Malysheva, T. I.
Ermak, A. A.
Stepanov, A. L.] Lomonosov Moscow State Univ, Fac Soil Sci, Moscow 119991, Russia
[Onipchenko, V. G.] Lomonosov Moscow State Univ, Fac Biol, Moscow 119991, Russia
[Menyailo, O. V.] Russian Acad Sci, Siberian Branch, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Makarov, M.I.; Malysheva, T.I.; Ermak, A.A.; Onipchenko, V.G.; Stepanov, A.L.; Menyailo, O.V.