Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / G. G. Polyakova, V. V. Stasova, N. V. Pashenova // Russ. J. Plant Physiol. - 2011. - Vol. 58, Is. 5. - P819-827, DOI 10.1134/S1021443711050177. - Cited References: 23. - This work was supported by the Russian Foundation for Basic Research, project no. 09-04-09030. . - 9. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Ophiostomatoid fungi colonize the conducting tissues of conifer stems, the phloem and the xylem. These pathogenic fungi penetrate into the stem through injuries made by xylophagous insects vectoring these pathogens. In this study the response of the phloem of Scotch pine (Pinus sylvestris L.) to wounding (treatment 1) was compared with the response to wounding combined with application of high-molecular-weight compounds isolated from the mycelium of the ophiostomatoid fungus Ceratocystis laricicola Redfern & Minter (treatment 2). Both treatments induced the appearance of necrosis in the inner bark, the formation of periderm separating living and dead tissues, and formation of the callus alongside the wound perimeter. In addition, the bark accumulated lignin, bound proanthocyanidins, and resins, with a parallel decrease in the content of free proanthocyanidins, low-molecular-weight carbohydrates, and non-lignin components of the cell wall (P > 0.95). The size of necrotic spots, as well as changes in the content of most substances, were significantly higher in the treatment 2 than in the treatment 1 (P > 0.95). The accumulation of lignin in cell walls of phloem sieve cells was delayed in the treatment 2 as compared with that in the treatment 1. This suggested that the mycelial extract temporarily inhibited lignification at the early stage of the wound response. This disturbance of the cell wall protective transformation led to the hypothesis that the fungal suppressors retard the repair of inner bark injured by insects, thereby favoring the invasion of conifer tissues by ophiostomatoid fungi.

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Держатели документа:
[Polyakova, G. G.
Stasova, V. V.
Pashenova, N. V.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Stasova, V.V.; Pashenova, N.V.

[Text] / G. G. Polyakova [et al.] // Russ. J. Plant Physiol. - 2008. - Vol. 55, Is. 4. - P496-502, DOI 10.1134/S1021443708040109. - Cited References: 25 . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: The elicitor activity of compounds extracted from the mycelia of six species of phytopathogenic fungi was assessed from the sizes of necrotic lesions on the external surface of the living trunk phloem of five coniferous species inhabiting Siberia: Siberian larch (Larix sibirica L.), Scotch pine (Pinus sylvestris L.), Siberian spruce (Picea obovata Ledeb.), Siberian fir (Abies sibirica L.), and cedar pine (Pinus sibirica (Rupr.) Mayr.). The compounds for inoculation were extracted from the mycelium of ascomycetes imperfect, and basidium fungi; the living mycelia of these fungi were also used. The fungal extract or mycelium was placed into the hollows 7 mm in diameter in the trunk bark. Infection triggered the formation of hypersensitivity necrotic lesions in the inner bark exceeding in size those appeared after control wounding of four tree species (larch, pine, spruce, and cedar); fir was an exclusion. In experiments with tree trunks and conifer calluses, a dependence of immune response parameters (the sizes of necrotic lesions and the content of lignin and bound proanthocyanidins) on the quantity of the fungal preparation was elucidated. The largest necrotic lesions appeared after injection of 500 mu g of the fungal preparation into the hollow in the trunk, and its higher quantities did not increase the indices measured. The size of the necrotic lesion on the trunk bark is supposed to be used as a promising index characterizing the level of tree immunity and tolerance under various ecological conditions.

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Держатели документа:
[Polyakova, G. G.
Pashenova, N. V.
Polyakov, V. I.
Zrazhevskaya, G. K.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Pashenova, N.V.; Polyakov, V.I.; Zrazhevskaya, G.K.

[Text] / A. S. Belorussova, I. N. Tret'yakova // Russ. J. Dev. Biol. - 2008. - Vol. 39, Is. 2. - P83-91, DOI 10.1134/S1062360408020045. - Cited References: 33 . - 9. - ISSN 1062-3604РУБ Developmental Biology

Аннотация: Somatic embryogenesis was induced in Siberian larch by in vitro culturing zygotic embryos at different developmental stages. Cultures were grown in modified Murashige and Skoog medium supplemented with hormones 2,4-dichlorophenoxyacetic acid (2 mg/l) and 6-benzylaminopurine (0.5-1 mg/l). The success of somatic embryogenesis in this species depended on the tree genotype and developmental stage of embryos used for culturing. Somatic embryogenesis from immature zygotic embryos at the stage of cotyledon initiation was most active. After 5-10 days, such embryos formed the embryogenic tissue including two cell types-elongated highly vacuolated embryonic tubes and small embryonic cells. Somatic embryos were isolated from proliferating embryogenic tissues after 2 months of culture.

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Держатели документа:
[Belorussova, A. S.
Tret'yakova, I. N.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

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Belorussova, A.S.; Tret'yakova, I.N.

[Text] / G. F. Antonova [et al.] // Russ. J. Plant Physiol. - 2005. - Vol. 52, Is. 1. - P83-92, DOI 10.1007/s11183-005-0013-x. - Cited References: 29 . - 10. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: The contents of ascorbic acid (AA) and its oxidized form, dehydroascorbic acid (DHA), were assessed as related to the tracheid differentiation in the course of early and late wood development in the Siberianlarch (Larix sibirica Ldb.) trees. The samples of the cambium, cell enlargement zone and mature cells were collected at the successive developmental stages by scraping tissues off layer by layer from trunk segments of the 20-year-old trees according to anatomical and histochemical criteria. While cambium initials were rapidly dividing, the AA contents per dry weight and per cell considerably exceeded the corresponding values characteristic of the late xylem development; such difference corresponded to the higher number of early tracheids per annual ring, as compared to the late tracheids. The AA content decreased as cells enlarged. The radial growth of the early wood tracheids, as compared to the late wood tracheids, was accompanied with a threefold increase in the AA and a decline in the DHA contents. The AA/DHA ratio was in line with the early tracheid enlargement. The maximum AA content was observed at the early stage of the secondary cell wall thickening in the tracheids of early and late xylem preceding lignification. During this stage of early wood development, the DHA content exceeded sixfold the corresponding value in the late xylem; as a result, the initial rates of lignification were different in two tissues. The rate of lignification in a newly developing layer of the early xylem increased gradually and was the highest in the completely differentiated tracheids. In the late xylem, the lignification rate was at its highest at the very beginning and then declined in the course of tracheid maturation. The dissimilar patterns of lignification in the early and late xylem were primarily associated with the DHA content, which increased in the early xylem and decreased in the maturing late xylem. Thus, the AA content and its accessibility to oxidation in the growing and mature xylem cells exhibited the diverse developmental patterns in the early and late xylem: two tissues differed in the tracheid number and radial diameter as well as in the rate of lignification.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Antonova, G.F.; Chaplygina, I.A.; Varaksina, T.N.; Stasova, V.V.

[Text] / G. F. Antonova, I. A. Chapligina ; ed.: R Lagana, S Kurjatko, , S Kurjat // WOOD STRUCTURE AND PROPERTIES' 06 : ARBORA PUBLISHERS, 2006. - 5th International Symposium Wood Structure and Properties (SEP 03-06, 2006, Sliac Siehnica, SLOVAKIA). - P25-31. - Cited References: 22 . - 7. - ISBN 978-80-968869-4-4РУБ Materials Science, Paper & Wood

Аннотация: The deposition of cellulose, matrix polysaccharides and lignin during secondary cell wall development of early- and latewood in larch (Larix sibirica Ldb.) were studied using biochemical and histochemical methods. Active tissues at successive stages of secondary cell wall development were sampled from larch trunk and characterized by morphological parameters and the content of cellulose, lignin and matrix hemicelluloses, being the structural base for lignification. Matrix polysaccharides were divided by their solubility in 4 % and 24 % KOH and neutral medium. All data were accounted per dry weight and per cell. The amount of biomass (as cell wall cross-section area) deposited before lignification was more in earlywood tracheids than in latewood ones. In contrast. the amount of cellulose at this stage was more in latewood tracheids. The intensity of cellulose synthesis was the highest at the initial stage of lignin deposition in both earlywood and latewood and then declined towards to mature xylem. The intensity of lignin deposition was the highest at the last stage of secondary cell wall development during earlywood formation and from the outset of latewood lignification, dropping towards mature xylem. The contents of A and B hemicellulose fractions linked or not with cellulose, before and after beginning of lignification were different during earlywood and latewood formation. Before lignin deposition, the content of B-4 fraction in both earlywood and latewood was practically equal as well as of B-24, while the amount of A-4 fraction before lignification of early xylem was two times more than that of latewood. This shows different matrix base for the beginning of lignification. All together can be the reason for different physicochemical properties of two types of wood in larch.

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Держатели документа:
Russian Acad Siences, SB, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Antonova, G.F.; Chapligina, I.A.; Lagana, R \ed.\; Kurjatko, S \ed.\; Kurjat, , S \ed.\

/ V. P. Zhelifonova [et al.] // Appl. Biochem. Microbiol. - 2019. - Vol. 55, Is. 3. - P277-283, DOI 10.1134/S0003683819030153. - Cited References:18 . - ISSN 0003-6838. - ISSN 1608-3024РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: Secondary metabolites of the basidiomycetes of Armillaria borealis Marxm. and Korhonen, A.cepistipes Velen., A. gallica Marxm., A. ostoyae (Romagn.) Herink, and A. sinapina Berube and Dessur isolated in Southern Siberia (Krasnoyarsk region and Tyva Republic) and in the Far East (Sikhote-Alin) were studied. Metabolites belonging to the class of protoilludene sesquiterpene aryl esters of the melleolides group have been identified in the species A. borealis, A. cepistipes, and A. sinapina. The strains differ in the spectrum of synthesized melleolides. A. borealis strain 74g synthesized a wider range of melleolides than other strains of the species: melleolides B, C, D and H, melledonals B and C, 5'-O-methylmelledonal, 13-hydroxy-5'-O-methylmelledonal, and armillarinin. It was shown that the composition of the medium influenced the amount of synthesized metabolites. All of the studied strains synthesizing melleolides exhibited a toxicogenic and phytopathogenic effect on seeds and seedlings of Picea abies (L.) H. Karst. and Abies sibirica Ledeb. A significant decrease in seed germination energy, laboratory and ground seed germination, the development of the root system of conifer seedlings was revealed. The maximum inhibitory effect was shown by the culture fluid of A. borealis 74g with the greatest amounts and variety of melleolides in the metabolome profile.

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Держатели документа:
Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Zhelifonova, V. P.; Antipova, T. V.; Litvinova, E. A.; Baskunov, B. P.; Litovka, Yu. A.; Pavlov, I. N.; Kozlovsky, A. G.

/ G. G. Polyakova, N. V. Pashenova, V. A. Senashova // Biol. Bull. - 2020. - Vol. 47, Is. 1. - P49-55, DOI 10.1134/S1062359020010100. - Cited References:28. - This work was supported by the Russian Foundation for Basic Research, project no. 15-04-06575. . - ISSN 1062-3590. - ISSN 1608-3059РУБ Biology

Аннотация: In the long-term explosion of mass reproduction of the larch casebearer (Coleophora sibiricella Falkovitsh) in a pure larch stand, the stem phloem responses of larch trees (Larix sibirica Ledeb.) to wounding damage or inoculation of the trunk with the fungal inductor were studied. The mycelium of the ophiostomatoid fungus Ceratocystis laricicola Redfern & Minter and its extractive and the protein fraction obtained from the extractive were used as a fungal inductor. The content of lignin, starch, and condensed tannins (proanthocyanidins) in the phloem was determined at the beginning of the experiment (zero days), and after seven and 14 days. Using ANOVA analysis, it was shown that the response parameters of the phloem did not depend on the kind of the fungal inoculum (P = 0.918), but depended on other factors (P

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Держатели документа:
Sukachev Inst Forest, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Polyakova, G. G.; Pashenova, N. V.; Senashova, V. A.; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [15-04-06575]

/ F. Gao, C. X. Peng, H. Wang [et al.] // Forests. - 2020. - Vol. 11, Is. 9. - Ст. 912, DOI 10.3390/f11090912. - Cited References:37. - The work was supported by the National Key R&D Program of China (2017YFD0600600), and the Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University, 2016C01). . - ISSN 1999-4907РУБ Forestry

Аннотация: Korean pine is the dominant species of Korean pine forests. It is an economically valuable species that yields oil, high-quality timber and nuts, and it offers great prospects for further development. Complete regenerated plants of Korean pine were obtained via somatic embryogenesis using megagametophytes as the explant. The seeds of 27 families of Korean pine were collected to induce embryogenic lines. We compared the effects of explant collection time, family and medium components (concentrations of sucrose, plant growth regulators and acid-hydrolyzed casein) on embryogenic lines induction. The effects of plant growth regulators and L-glutamine contents on the proliferation and maturation of embryogenic cell lines were studied, and the germinating ability of different cell lines was evaluated. The embryogenic lines induction percentage of Korean pine reached 33.33%. When 4.52 mu mol center dot L(-1)2,4-D and 2.2 mu mol center dot L(-1)6-BA were added to the medium of embryogenic lines proliferation, the ability of embryo maturation was the best (cell line 001#-100 was 135.71 center dot g(-1)fresh weight). Adding 1-1.5g L-1L-glutamine to the proliferation medium can improve the ability of embryo maturation (cell line 001#-100 was 165.63 center dot g(-1)fresh weight). The germination percentage of the three cell lines tested was significant, and the highest was 66%. We report on successful regeneration and cryopreservation methods for somatic embryos of Korean pine. This technology could be used to propagate the excellent germplasm resources of Korean pine and to establish multi-varietal forestry.

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Держатели документа:
Northeast Forestry Univ, Sch Forestry, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China.
RAS, Forest Siberian Branch, Sukachev Inst, Lab Forest Genet & Breeding,Inst Russian Acad Sci, Krasnoyarsk 660036, Russia.
Timiryazev Russian Acad Sci, Dept Cell Biol, Moscow 127276, Russia.
Timiryazev Russian Acad Sci, Inst Plant Physiol KA, Moscow 127276, Russia.
Lomonosov Moscow State Univ, Fac Biol, Dept Plant Physiol, Moscow 119991, Russia.
State Forestry & Grassland Adm Engn Technol Res C, Harbin 150040, Peoples R China.

Доп.точки доступа:
Gao, Fang; Peng, Chunxue; Wang, Hao; Tretyakova, Iraida Nikolaevna; Nosov, Alexander Mikhaylovich; Shen, Hailong; Yang, Ling; National Key R&D Program of China [2017YFD0600600]; Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) [2016C01]

/ T. V. Antipova, V. P. Zhelifonova, Y. A. Litovka [et al.] // iForest. - 2022. - Vol. 15. - P38-46, DOI 10.103832/ifor3840014. - Cited References:28 . - ISSN 1971-7458РУБ Forestry

Аннотация: Basidiomycetes Armillaria infect deciduous, coniferous and fruit trees, causing enormous economic damage. The role of secondary metabolites (tricyclic sesquiterpene aryl esters - melleolides) in the life cycle and pathogenesis of Armillaria is under active investigation. To date, not all species of Armillaria have been tested for the biosynthesis of melleolides. We investigated the secondary metabolite profiles of six root-pathogenic species of the genus Armillaria (A. borealis Marxmuller & Korhonen, A. cepistipes Velenovsky, A. gallica Marxm, A. mellea (Vahl) P. Kummer, A. sinapina Berube & Dessur, A. ostoyae (Romagn.) Herink) distributed in Siberia (South Krasnoyarsk Krai, Republic of Tyva, Republic of Khakassia, Taimyr Peninsula), Russian Far East (Sikhote-Alin) and Crimea (Krymsky National Park, Chatyr-Dag Mountain Lower Plateau). A total of 15 compounds were identified in the metabolome profile. Two compounds (melleolide D and melledonal C) are synthesized by all investigated strains irrespective of their geographic location and host plant. The maximum spectrum of melleolides (7-8 compounds) was found in isolates of A. borealis, A. gallica, A. sinapina, A. ostoyae. In submerged culture, the maximum accumulation of melleolides varied from 2 up to 239 mg l(-1). A mixture of melleolide D and melledonal C (1:1) synthesized by the most productive strain A. mellea Cr2-17 was first found to have a phytotoxic action on the growth parameters of the callus culture Populus balsamifera and 10-day-old conifer seedlings. A 0.5% concentration of melleolides caused a credible decrease of P. balsamifera callus raw biomass; a decrease of the viability of Larix sibirica and, which is especially significant, Pinus sylvestris seedlings; inhibition of stem and root growth processes; dechromation of foliage; loss of turgor. The occurrence of a broad range of melleolides in the metabolome profile and two common compounds in all investigated strains, with a phytotoxic action at their sufficiently high concentration, enables considering the synthesis of melleolides by Armillaria fungi as one of the possible mechanisms of their pathogenicity efficiently realized in strains characterized by overproduction of melleolides under natural conditions.

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Держатели документа:
Russian Acad Sci, GK Skryabin Inst Biochem & Physiol Microorganisms, FRC Pushchino Ctr Biol Res, 5 Prosp Nauki, Pushchino 142290, Moscow Region, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, FRC KSC, Siberian Branch, 50 Akad Gorodok Str, Krasnoyarsk 660036, Russia.
FSBEIHE MF Reshetnev Siberian State Univ Sci & Te, 82 Prosp Mira, Krasnoyarsk 660037, Russia.
FSBEIHE Krasnoyarsk State Agr Univ, 90 Prosp Mira, Krasnoyarsk 660049, Russia.

Доп.точки доступа:
Antipova, Tatyana, V; Zhelifonova, Valentina P.; Litovka, Yulia A.; Pavlov, Igor N.; Baskunov, Boris P.; Kokh, Zhanna A.; Makolova, Polina, V; Timofeev, Anton A.; Kozlovsky, Anatoly G.