Рубрики:
EMBRYOGENESIS
CULTURES
IMMATURE
GROWTH
LARIX
CELLS
Кл.слова (ненормированные):
cleavage polyembryony -- embryogenic tissue -- somatic embryos -- Siberian larch
EMBRYOGENESIS
CULTURES
IMMATURE
GROWTH
LARIX
CELLS
Кл.слова (ненормированные):
cleavage polyembryony -- embryogenic tissue -- somatic embryos -- Siberian larch
Аннотация: Somatic embryogenesis was induced in Siberian larch by in vitro culturing zygotic embryos at different developmental stages. Cultures were grown in modified Murashige and Skoog medium supplemented with hormones 2,4-dichlorophenoxyacetic acid (2 mg/l) and 6-benzylaminopurine (0.5-1 mg/l). The success of somatic embryogenesis in this species depended on the tree genotype and developmental stage of embryos used for culturing. Somatic embryogenesis from immature zygotic embryos at the stage of cotyledon initiation was most active. After 5-10 days, such embryos formed the embryogenic tissue including two cell types-elongated highly vacuolated embryonic tubes and small embryonic cells. Somatic embryos were isolated from proliferating embryogenic tissues after 2 months of culture.
Полный текст,
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Держатели документа:
[Belorussova, A. S.
Tret'yakova, I. N.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia
Доп.точки доступа:
Belorussova, A.S.; Tret'yakova, I.N.