Рубрики:
ARTIFICIAL AFFORESTATION EXPERIMENT
PARTICULATE METHANE MONOOXYGENASE
FOREST SOILS
MICROBIAL COMMUNITIES
OXIDIZING BACTERIA
ECOSYSTEM FUNCTION
PLANT DIVERSITY
EUROPEAN BEECH
NORWAY SPRUCE
FATTY-ACIDS
Кл.слова (ненормированные):
Atmospheric methane -- High-affinity methanotrophs -- C-13-Labeling -- Microbial diversity -- Net N mineralization -- Net nitrification -- PLFA -- Tree species
ARTIFICIAL AFFORESTATION EXPERIMENT
PARTICULATE METHANE MONOOXYGENASE
FOREST SOILS
MICROBIAL COMMUNITIES
OXIDIZING BACTERIA
ECOSYSTEM FUNCTION
PLANT DIVERSITY
EUROPEAN BEECH
NORWAY SPRUCE
FATTY-ACIDS
Кл.слова (ненормированные):
Atmospheric methane -- High-affinity methanotrophs -- C-13-Labeling -- Microbial diversity -- Net N mineralization -- Net nitrification -- PLFA -- Tree species
Аннотация: Plant species exert strong effects on ecosystem functions and one of the emerging, and difficult to test hypotheses, is that plants alter soil functions through changing the community structure of soil microorganisms. We tested the hypothesis for atmospheric CH4 oxidation by using soil samples from a Siberian afforestation experiment and exposing them to C-13-CH4. We determined the activity of the soil methanotrophs under different tree species at three levels of initial CH4 concentration (30, 200 and 1000 ppm) thus distinguishing the activities of low- and high-affinity methanotrophs. Half of the samples were incubated with C-13-enriched CH4 (99.9%) and half with C-12-CH4. This allowed an estimation of the amount of C-13 incorporated into individual PLFAs and determination of PLFAs of methanotrophs involved in CH4 oxidation at the different CH4 concentrations. Tree species strongly altered the activity of atmospheric CH4 oxidation without appearing to change the composition of high-affinity methanotrophs as evidenced by PLFA C-13 labeling. The low diversity of atmospheric CH4 oxidizers, presumably belonging to the UCS alpha group, may explain the lack of tree species effects on the composition of soil methanotrophs. We submit that the observed tree species effects on atmospheric CH4 oxidation indicate an effect on biomass or cell-specific activities rather than by a community change and this may be related to the impact of the tree species on soil N cycling. (C) 2009 Elsevier Ltd. All rights reserved.
Полный текст,
WOS,
Scopus
Держатели документа:
[Menyailo, Oleg V.] SB RAS, VN Sukachev Inst Forest, Krasnoyarsk 660036, Russia
[Menyailo, Oleg V.
Conrad, Ralf] Max Planck Inst Terr Microbiol, D-35043 Marburg, Germany
[Abraham, Wolf-Rainer] Helmholtz Ctr Infect Res, D-38124 Braunschweig, Germany
Доп.точки доступа:
Menyailo, O.V.; Abraham, W.R.; Conrad, R...