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    Somaclonal variation of haploid in vitro tissue culture obtained from Siberian larch (Larix sibirica Ledeb.) megagametophytes for whole genome de novo sequencing
[Text] / K. V. Krutovsky [et al.] // In Vitro Cell. Dev. Biol.-Plant. - 2014. - Vol. 50, Is. 5. - P655-664, DOI 10.1007/s11627-014-9619-z. - Cited References: 77. - This study was supported by Research Grant No. 14.Y26.31.0004 from the Government of the Russian Federation. . - ISSN 1054-5476. - ISSN 1475-2689
РУБ Plant Sciences + Cell Biology + Developmental Biology

Аннотация: The objective of this study was to obtain a genetically stable haploid in vitro-derived line from Siberian larch (Larix sibirica Ledeb.) using megagametophyte explants, which then could be used for different molecular genetic studies, including whole genome de novo sequencing. However, cytogenetic analysis and genotyping of 11 microsatellite loci showed high levels of genomic instability and a high frequency of mutation in the obtained megagametophyte-derived callus cultures. All cultures contained new mutations in one or more microsatellite loci.

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Держатели документа:
[Krutovsky, Konstantin V.] Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgen Inst, D-37077 Gottingen, Germany
[Krutovsky, Konstantin V.] Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA
[Krutovsky, Konstantin V.] Russian Acad Sci, NI Vavilov Inst Gen Genet, Moscow 119333, Russia
[Krutovsky, Konstantin V.
Tretyakova, Iraida N.
Oreshkova, Nataliay V.
Vaganov, Eugene A.] Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia
[Tretyakova, Iraida N.
Oreshkova, Nataliay V.
Pak, Maria E.
Kvitko, Olga V.] Russian Acad Sci, Lab Forest Genet & Select, VN Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia
[Vaganov, Eugene A.] Siberian Fed Univ, Dept Ecol & Environm Studies, Inst Econ Management & Environm Studies, Krasnoyarsk 660041, Russia
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Доп.точки доступа:
Krutovsky, K.V.; Tretyakova, I.N.; Oreshkova, N.V.; Pak, M.E.; Kvitko, O.V.; Vaganov, E.A.; Government of the Russian Federation [14.Y26.31.0004]