Рубрики:
PHASEOLUS-VULGARIS L
CELL-WALLS
POLYSACCHARIDES
HYPOCOTYLS
LECTINS
Кл.слова (ненормированные):
SIBERIAN LARCH -- XYLEM -- ARABINOGALACTAN PROTEINS -- LOCATION
PHASEOLUS-VULGARIS L
CELL-WALLS
POLYSACCHARIDES
HYPOCOTYLS
LECTINS
Кл.слова (ненормированные):
SIBERIAN LARCH -- XYLEM -- ARABINOGALACTAN PROTEINS -- LOCATION
Аннотация: We conducted a comparative study on the composition of water soluble carbohydrate-protein complexes of primary cell walls and cytoplasm in xylem cells of Siberian larch (Larix sibirica Ldb.). Preparations were obtained in the process of isolating protoplasts from xylem cells at the stage of elongation. Water-soluble high-molecular-weight compounds were fractionated on DEAE cellulose. It was found that the dominant fraction in composition of these compounds consists of a carbohydrate-protein complex. Comparison of the composition of neutral sugars, uronic acids, and amino acids of these complexes showed that water-soluble arabinogalactan proteins were absent in the cytoplasm, but present in the solution after plasmolysis. They were bound with pectins and arabinoxylans, detected in solutions obtained after isolation and purification of protoplasts, and capable of forming a gel at low water content and low temperature. It is hypothesized that arabinogalactan proteins are components of primary cell walls, that they are located for the most part on the surface, and that their solubility in an aqueous medium depends upon binding with pectins of the middle plate and with other cell wall components.
WOS
Доп.точки доступа:
ANTONOVA, G.F.; STASOVA, V.V.