Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / A. S. Belorussova, I. N. Tret'yakova // Russ. J. Dev. Biol. - 2008. - Vol. 39, Is. 2. - P83-91, DOI 10.1134/S1062360408020045. - Cited References: 33 . - 9. - ISSN 1062-3604РУБ Developmental Biology

Аннотация: Somatic embryogenesis was induced in Siberian larch by in vitro culturing zygotic embryos at different developmental stages. Cultures were grown in modified Murashige and Skoog medium supplemented with hormones 2,4-dichlorophenoxyacetic acid (2 mg/l) and 6-benzylaminopurine (0.5-1 mg/l). The success of somatic embryogenesis in this species depended on the tree genotype and developmental stage of embryos used for culturing. Somatic embryogenesis from immature zygotic embryos at the stage of cotyledon initiation was most active. After 5-10 days, such embryos formed the embryogenic tissue including two cell types-elongated highly vacuolated embryonic tubes and small embryonic cells. Somatic embryos were isolated from proliferating embryogenic tissues after 2 months of culture.

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Держатели документа:
[Belorussova, A. S.
Tret'yakova, I. N.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Belorussova, A.S.; Tret'yakova, I.N.

[Text] / I. N. Tret'yakova [et al.] // Russ. J. Plant Physiol. - 2009. - Vol. 56, Is. 5. - P647-653, DOI 10.1134/S1021443709050100. - Cited References: 35. - This work was supported by the Russian Foundation for Basic Research, project no. 06-04-08040-ofi. . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Androgenesis in vitro in plants is a phenomenon of developmental switching of male generative cells, microspores, from their normal gametophytic to sporophytic pathway. We obtained androgenic callus and embryoids (embryo-like structures derived from microspores) of the conifer plant, Siberian larch (Larix sibirica Ledeb.) in the in vitro culture. The immune-enzyme analysis of the hormonal balance of larch androgenic cultures showed a substantial increase in the content of all phytohormones, especially cytokinins and ABA, as compared with initial explants (microstrobiles). This was evidently related to active cell divisions and embryoid formation. A comparison of androgenic cultures derived from trees nonifested and infested with larch gall midges (Dasineura rozhkovi Main. et Nik.) revealed a cytokinin content increase (by two times) and an ABA content decrease (by two times), which indicates more intense growth of cultures derived from healthy trees. Phytohormone content in the androgenic callus was compared with their accumulation in the embryos of larch seeds harvested from noninfested trees. We concluded that successful growth of androgenic cultures and embryoid formation demand an additional medium supplement with auxins (not more than 0.5 mg/l).

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Держатели документа:
[Tret'yakova, I. N.
Ivanitskaya, A. C.
Barsukova, A. V.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia
[Ivanova, A. N.] Siberian Fed Univ, Krasnoyarsk, Russia

Доп.точки доступа:
Tret'yakova, I.N.; Ivanitskaya, A.C.; Ivanova, A.N.; Barsukova, A.V.; Russian Foundation for Basic Research [06-04-08040-ofi]