Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ I. N. Tret'yakova, A. V. Barsukova // Russ. J. Dev. Biol. - 2012. - Vol. 43, Is. 6. - P353-361, DOI 10.1134/S1062360412060082. - Cited References: 32. - This work was supported by the Russian Foundation for Basic Research, project no. 11-04-00281 and Integration Project no. 53 (Genofond of Conifers). . - 9. - ISSN 1062-3604РУБ Developmental Biology

Аннотация: Embryogenic callus formation in different larch species from Siberia (Larix sibirica, L. gmelinii, and L. sukaczewii) was carried out on MSGm medium supplemented with growth regulators (2.4-D and BAP) and followed one and the same scheme: elongation of somatic cells and their asymmetric division with formation of initial and tube cells. The cells of embryo initial underwent sequential divisions and formed embryonic globules which caused the formation of somatic embryos. Somatic embryos became mature and germinated by addition of ABA and PEG into the medium. Long-term proliferating cell lines and regenerant plants were obtained in Sukachev larch and its hybrid with Siberian larch. The success of somatic embryogenesis depended on the genotype of the donor tree.

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Держатели документа:
[Tret'yakova, I. N.
Barsukova, A. V.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Tret'yakova, I.N.; Barsukova, A.V.

/ I. N. Tretyakova, A. S. Shuklina, M. E. Park [et al.] // Cytologia. - 2021. - Vol. 86, Is. 1. - P55-60, DOI 10.1508/cytologia.86.55. - Cited References:24. - The reported study was funded by RFBR, the Government of the Krasnoyarsk Territory and the Regional Science Foundation, project number 19-44-240009. . - ISSN 0011-4545РУБ Cell Biology + Genetics & Heredity

Аннотация: The contents of auxin (indole-3-acetic acid, IAA), cytokinins, and abscisic acid (ABA) were determined in explants (megagametophyte and embryo), proliferating embryonal-suspensor mass (ESM), and non-embryogenic callus of Pinus sibirica and Larix sibirica. Furthermore, the localization of hormones in the cells was studied with immunohistochemical analysis. It was shown that the formation of embryogenic cultures in both species is associated with the content of phytohormones and their localization in cells. In L. sibirica the content of IAA in ESM was about 100 times higher than in non-embryogenic calli. At the same time, a low ABA content was characteristic of embryogenic cultures. Non-embryogenic callus contains an increased content of cytokinins and ABA. The transition of somatic cells to the path of embryogenic development is characterized by elongation of cells, their asymmetric division, and localization of IAA at one of the ends of elongated cells. Non-embryogenic callus consisted of isodiametric, actively dividing cells.

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Держатели документа:
Russian Acad Sci, Fed Res Ctr, Sukachev Inst Forest, Siberian Branch,Krasnoyarsk Sci Ctr SB RAS, Akademgorodok 50-28, Krasnoyarsk 660036, Russia.
Northeast Forestry Univ, Sch Forestry, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China.
Russian Acad Sci, Ufa Res Ctr RAS, Inst Biol, Pr T Oktyabrya 69, Ufa 450054, Russia.

Доп.точки доступа:
Tretyakova, Iraida N.; Shuklina, Alla S.; Park, Maria E.; Yang, Ling; Akhiyarova, Guzel R.; Kudoyarova, Guzel R.; RFBRRussian Foundation for Basic Research (RFBR); Government of the Krasnoyarsk Territory; Regional Science Foundation [19-44-240009]