Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / G. F. Antonova, T. V. Zheleznichenko, V. V. Stasova // Russ. J. Dev. Biol. - 2011. - Vol. 42, Is. 4. - P238-246, DOI 10.1134/S1062360411020032. - Cited References: 48. - This work was supported by the Russian Foundation for Basic Research, project no. 06-04-49501. . - 9. - ISSN 1062-3604РУБ Developmental Biology

Аннотация: The content and composition of alcohol soluble phenolic acids (PhAs) were studied during cell xylem growth in course of wood annual increment formation in the trunks of Scots pine. Cells of the cambium zone, two stages of expansion growth, and outset of secondary thickening zone (before lignification) within the period of formation of early wood xylem were subsequently isolated from trunk segments of 25-year-old trees with constant anatomical and histochemical control. The amount of free and bound forms of phenolic acids extracted from tissues by 80% ethanol, as well as their ethers and esters, were calculated both per dry weight and per cells. The substantial alteration in content, proportion of fractions and composition of acids has been found between the cambium zone and the outset of secondary thickening of tracheids, and the character of variation depended on the calculation method. The amount of free and bound PhAs and esters and especially ethers calculated per cell had increased at the first stage of extension growth, reduced at the second, and increased in the outset of secondary wall deposition. The pool of bound acids was more than acids by 2-5 times depending on the stage of development of the cells. Sinapic and ferulic acids dominate among free hydroxycinnamic acids. The composition and the content of hydroxycinnamic acids in esters and ethers also depended on the stage of development of the cells. p-Coumaric and sinapic acids were the main aglycons in ethers in the cambium and sinapic and caffeic acids were in the other stages. The esters from cambium included mostly p-coumaric acid and those at other stages of development were sinapic and ferulic acids. The esters included benzoic acid at the first stages of growth. The pool of these esters decreased from the first phase of growth until the outset of cell wall thickening. The level of free benzoic acid increased respectively.

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Держатели документа:
[Antonova, G. F.
Zheleznichenko, T. V.
Stasova, V. V.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Antonova, G.F.; Zheleznichenko, T.V.; Stasova, V.V.

[Text] / I. N. Tret'yakova [et al.] // Russ. J. Plant Physiol. - 2009. - Vol. 56, Is. 5. - P647-653, DOI 10.1134/S1021443709050100. - Cited References: 35. - This work was supported by the Russian Foundation for Basic Research, project no. 06-04-08040-ofi. . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Androgenesis in vitro in plants is a phenomenon of developmental switching of male generative cells, microspores, from their normal gametophytic to sporophytic pathway. We obtained androgenic callus and embryoids (embryo-like structures derived from microspores) of the conifer plant, Siberian larch (Larix sibirica Ledeb.) in the in vitro culture. The immune-enzyme analysis of the hormonal balance of larch androgenic cultures showed a substantial increase in the content of all phytohormones, especially cytokinins and ABA, as compared with initial explants (microstrobiles). This was evidently related to active cell divisions and embryoid formation. A comparison of androgenic cultures derived from trees nonifested and infested with larch gall midges (Dasineura rozhkovi Main. et Nik.) revealed a cytokinin content increase (by two times) and an ABA content decrease (by two times), which indicates more intense growth of cultures derived from healthy trees. Phytohormone content in the androgenic callus was compared with their accumulation in the embryos of larch seeds harvested from noninfested trees. We concluded that successful growth of androgenic cultures and embryoid formation demand an additional medium supplement with auxins (not more than 0.5 mg/l).

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Держатели документа:
[Tret'yakova, I. N.
Ivanitskaya, A. C.
Barsukova, A. V.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia
[Ivanova, A. N.] Siberian Fed Univ, Krasnoyarsk, Russia

Доп.точки доступа:
Tret'yakova, I.N.; Ivanitskaya, A.C.; Ivanova, A.N.; Barsukova, A.V.; Russian Foundation for Basic Research [06-04-08040-ofi]

[Text] / G. F. Antonova, V. V. Stasova, T. N. Varaksina // Russ. J. Plant Physiol. - 2009. - Vol. 56, Is. 2. - P190-199, DOI 10.1134/S1021443709020071. - Cited References: 30. - This work was supported by the Russian Foundation for Basic Research, project no. 06-04-49501. . - 10. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Changes in the levels of ascorbic acid (AA), its oxidized form, dehydroascorbic acid (DHA), and uronic acids as initial precursors for the AA synthesis were studied as related to the degree of xylem and phloem cell development in the course of early and late wood formation in the trunks of Scots pine (Pinus sylvestris L.). The cells of mature and conducting phloem, cambial zone, differently developed cells in the zones of cell enlargement and maturation were obtained by successive scraping tissue layers from trunk segments of 20-25-year-old trees; tissue identification was checked anatomically and histochemically. The contents of compounds tested were calculated per dry weight and per cell basis. We found great differences in the contents of AA and DHA and also in their ratio in dependence of the wood type developing in the pine trunks during growth period and on the stage of differentiation of xylem and phloem cells. Changes in the AA content during xylem cell differentiation were accompanied by changes in the content of uronic acids. The amounts of AA, DHA, and uronic acids were the highest at the stage of early lignification and reduced with tracheid maturation. The AA to DHA ratio changed differently in the course of early and late xylem lignification. It reduced from the start of lignification to the formation of early mature xylem and, in contrast, increased in mature late wood; this indicates a difference in the level of redox processes in these tissues.

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Держатели документа:
[Antonova, G. F.
Stasova, V. V.
Varaksina, T. N.] Russian Acad Sci, Sukachev Inst Forest, Siberian Div, Akad Gorodok, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Antonova, G.F.; Stasova, V.V.; Varaksina, T.N.; Russian Foundation for Basic Research [06-04-49501]