Рубрики:
PROTEOMIC ANALYSIS
PINE
CULTURES
PATHWAYS
EMBRYOS
SEEDS
Кл.слова (ненормированные):
Korean pine -- embryogenic lines -- somatic embryos -- regenerated plant -- megagametophytes -- cryopreservation
PROTEOMIC ANALYSIS
PINE
CULTURES
PATHWAYS
EMBRYOS
SEEDS
Кл.слова (ненормированные):
Korean pine -- embryogenic lines -- somatic embryos -- regenerated plant -- megagametophytes -- cryopreservation
Аннотация: Korean pine is the dominant species of Korean pine forests. It is an economically valuable species that yields oil, high-quality timber and nuts, and it offers great prospects for further development. Complete regenerated plants of Korean pine were obtained via somatic embryogenesis using megagametophytes as the explant. The seeds of 27 families of Korean pine were collected to induce embryogenic lines. We compared the effects of explant collection time, family and medium components (concentrations of sucrose, plant growth regulators and acid-hydrolyzed casein) on embryogenic lines induction. The effects of plant growth regulators and L-glutamine contents on the proliferation and maturation of embryogenic cell lines were studied, and the germinating ability of different cell lines was evaluated. The embryogenic lines induction percentage of Korean pine reached 33.33%. When 4.52 mu mol center dot L(-1)2,4-D and 2.2 mu mol center dot L(-1)6-BA were added to the medium of embryogenic lines proliferation, the ability of embryo maturation was the best (cell line 001#-100 was 135.71 center dot g(-1)fresh weight). Adding 1-1.5g L-1L-glutamine to the proliferation medium can improve the ability of embryo maturation (cell line 001#-100 was 165.63 center dot g(-1)fresh weight). The germination percentage of the three cell lines tested was significant, and the highest was 66%. We report on successful regeneration and cryopreservation methods for somatic embryos of Korean pine. This technology could be used to propagate the excellent germplasm resources of Korean pine and to establish multi-varietal forestry.
WOS
Держатели документа:
Northeast Forestry Univ, Sch Forestry, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China.
RAS, Forest Siberian Branch, Sukachev Inst, Lab Forest Genet & Breeding,Inst Russian Acad Sci, Krasnoyarsk 660036, Russia.
Timiryazev Russian Acad Sci, Dept Cell Biol, Moscow 127276, Russia.
Timiryazev Russian Acad Sci, Inst Plant Physiol KA, Moscow 127276, Russia.
Lomonosov Moscow State Univ, Fac Biol, Dept Plant Physiol, Moscow 119991, Russia.
State Forestry & Grassland Adm Engn Technol Res C, Harbin 150040, Peoples R China.
Доп.точки доступа:
Gao, Fang; Peng, Chunxue; Wang, Hao; Tretyakova, Iraida Nikolaevna; Nosov, Alexander Mikhaylovich; Shen, Hailong; Yang, Ling; National Key R&D Program of China [2017YFD0600600]; Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) [2016C01]