Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ N. A. Tikhonova, M. A. Polezhaeva, E. A. Pimenova // Russ. J. Genet. - 2012. - Vol. 48, Is. 10. - P985-992, DOI 10.1134/S1022795412100110. - Cited References: 41 . - 8. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: Using AFLP analysis, the genetic variation in four population samples of Rhododendron ledebourii, three such samples of Rh. dauricum, two samples of Rh. sichotense, and one sample of Rh. mucronulatum was examined. Application of three selective primer pairs provided genotyping of 87 accessions from the four species at 213 loci. An analysis of molecular variance revealed a high level of differences between all four species (F (ST) = 0.172). Populations within the species were also differentiated: for Rh. dauricum F (ST) = 0.245; Rh. ledebourii F (ST) = 0.264; Rh. sichotense F (ST) = 0.034. The highest Nei distance was observed for Rh. mucronulatum with Rh. dauricum and Rh. ledebourii (D = 0.057 and 0.069, respectively). Similarity was observed between the species pairs of Rh. ledebourii-Rh. dauricum (0.032) and Rh. sichotense-Rh. mucronulatum (0.034). The Far Eastern species Rh. sichotense and Rh. mucronulatum were characterized by an elevated level of intrapopulation variation. The data from the AFLP-based analysis of differentiation and variation that were thus obtained were compared with the literature data on the morphological criteria that differentiate the species of interest and those regarding the history of the formation of their ranges. The independence of all the species we examined was confirmed.

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[Tikhonova, N. A.] Russian Acad Sci, Sukachev Forest Inst, Krasnoyarsk 660036, Russia
[Polezhaeva, M. A.] Russian Acad Sci, Inst Plant & Anim Ecol, Ekaterinburg 620144, Russia
[Pimenova, E. A.] Abramov Sikhote Alin State Biosphere Reserve, Primorsky Krai 692150, Terney, Russia

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Tikhonova, N.A.; Polezhaeva, M.A.; Pimenova, E.A.

[Text] / A. Y. Larionova // Russ. J. Genet. - 2002. - Vol. 38, Is. 12. - P1391-1396, DOI 10.1023/A:1021639806123. - Cited References: 34 . - 6. - ISSN 1022-7954РУБ Genetics & Heredity

Аннотация: The main parameters of genetic variability have been determined in an isolated natural Scotch pine population from Chita oblast (Tsasuchei Forest) by analysis of 19 genes coding for nine enzymes: GDH, IDH, LAP, PGM, AAT, ADH, MDH, 6-PGD, and DIA. Polymorphic genes constituted 63.2% of all structural genes studied in the population at the 99% polymorphism criterion. The mean number of alleles per locus was 1.63. The observed and expected heterozygosities were 0.237 and 0.251, respectively. These estimates are close to the corresponding mean values for Scotch pine according to the data on 18 or more structural genes.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forestry, Krasnoyarsk 660036, Russia

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Larionova, A.Y.

[Text] / G. E. Rehfeldt [et al.] // Glob. Change Biol. - 2002. - Vol. 8, Is. 9. - P912-929, DOI 10.1046/j.1365-2486.2002.00516.x. - Cited References: 49 . - 18. - ISSN 1354-1013РУБ Biodiversity Conservation + Ecology + Environmental Sciences

Аннотация: Five population-specific response functions were developed from quadratic models for 110 populations of Pinus sylvestris growing at 47 planting sites in Eurasia and North America. The functions predict 13 year height from climate: degree-days > 5 degreesC; mean annual temperature; degree-days < 0 degreesC; summer-winter temperature differential; and a moisture index, the ratio of degree-days > 5 degreesC to mean annual precipitation. Validation of the response functions with two sets of independent data produced for all functions statistically significant simple correlations with coefficients as high as 0.81 between actual and predicted heights. The response functions described the widely different growth potentials typical of natural populations and demonstrated that these growth potentials have different climatic optima. Populations nonetheless tend to inhabit climates colder than their optima, with the disparity between the optimal and inhabited climates becoming greater as the climate becomes more severe. When driven by a global warming scenario of the Hadley Center, the functions described short-term physiologic and long-term evolutionary effects that were geographically complex. The short-term effects should be negative in the warmest climates but strongly positive in the coldest. Long-term effects eventually should ameliorate the negative short-term impacts, enhance the positive, and in time, substantially increase productivity throughout most of the contemporary pine forests of Eurasia. Realizing the long-term gains will require redistribution of genotypes across the landscape, a process that should take up to 13 generations and therefore many years.

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USDA, Forest Serv, Rocky Mt Res Stn, Moscow, ID 83843 USA
Russian Acad Sci, Sikachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

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Rehfeldt, G.E.; Tchebakova, N.M.; Parfenova, Y.I.; Wykoff, W.R.; Kuzmina, N.A.; Milyutin, L.I.

[Текст] / A. Y. LARIONOVA // Genetika. - 1995. - Vol. 31, Is. 9. - С. 1261-1267. - Cited References: 43 . - 7. - ISSN 0016-6758РУБ Genetics & Heredity

Аннотация: By means of electrophoresis followed by histochemical staining, water-soluble enzymes of megagametophytes and embryos of seeds collected from 42 trees in two natural populations of Picea obovata were studied. A detailed analysis of electrophoretic patterns of enzymes ADH, GDH, 6-PGD, IDH, MDH, LAP, EST, and GOT is presented. It is shown that the allozyme diversity of the enzymes assayed is coded for by at least 20 loci. Fine electrophoretic resolution was obtained for the products of 16 loci, four of them (Mdh-1, Mdh-2, Got-1, and Got-2) were monomorphic, while the remaining loci (Adh-1, Adh-2, 6-Pgd-2, 6-Pgd-3, Mdh-3, Idh-2, Lap-1, Lap-2, Est-1, Est-3, Got-3 and Gdh) appeared polymorphic. Segregation data confirms the monogenic inheritance of the allozyme variants revealed.


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LARIONOVA, A.Y.

/ E. I. Bondar [et al.] // BMC Bioinformatics. - 2019. - Vol. 20: 11th International Multiconference on Bioinformatics of Genome (AUG 20-25, 2018, Novosibirsk, RUSSIA). - Ст. 38, DOI 10.1186/s12859-018-2571-x. - Cited References:25. - The presented study was a part of the project "Genomic studies of major boreal coniferous forest tree species and their most dangerous pathogens in the Russian Federation" funded by the Government of the Russian Federation (grant No 14.Y26.31.0004). Publication costs are funded by the BioMed Central Membership of the University of Gottingen. . - ISSN 1471-2105РУБ Biochemical Research Methods + Biotechnology & Applied Microbiology

Аннотация: BackgroundThe main objectives of this study were sequencing, assembling, and annotation of chloroplast genome of one of the main Siberian boreal forest tree conifer species Siberian larch (Larix sibirica Ledeb.) and detection of polymorphic genetic markers - microsatellite loci or simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs).ResultsWe used thedata of the whole genome sequencing of three Siberian larch trees from different regions - theUrals, Krasnoyarsk, and Khakassia, respectively. Sequence reads were obtained using the Illumina HiSeq2000 in the Laboratory of Forest Genomics at the Genome Research and Education Center ofthe Siberian Federal University. The assembling was done using the Bowtie2 mapping program and the SPAdes genomic assembler. The genome annotation was performed using the RAST service. We used the GMATo program for the SSRs search, and the Bowtie2 and UGENE programs for the SNPs detection. Length of the assembled chloroplast genome was 122,561bp, which is similar to 122,474bp in the closely related European larch (Larix decidua Mill.). As a result of annotation and comparison of the data with theexisting data available only for three larch species - L. decidua, L. potaninii var. chinensis (complete genome 122,492bp), and L. occidentalis (partial genome of 119,680bp), we identified 110 genes, 34 of which represented tRNA, 4 rRNA, and 72 protein-coding genes. In total, 13 SNPs were detected; two of them were in the tRNA-Arg and Cell division protein FtsH genes, respectively. In addition, 23 SSR loci were identified.ConclusionsThe complete chloroplast genome sequence was obtained for Siberian larch for the first time. The reference complete chloroplast genomes, such as one described here, would greatly help in the chloroplast resequencing and search for additional genetic markers using population samples. The results of this research will be useful for further phylogenetic and gene flow studies in conifers.

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Держатели документа:
Siberian Fed Univ, Genome Res & Educ Ctr, Lab Forest Genom, Krasnoyarsk 660036, Russia.
Russian Acad Sci, VN Sukachev Inst Forest, Lab Forest Genet & Select, Siberian Branch, Krasnoyarsk 660036, Russia.
Georg August Univ Gottingen, Dept Forest Genet & Forest Tree Breeding, Busgenweg 2, D-37077 Gottingen, Germany.
Russian Acad Sci, Vavilov Inst Gen Genet, Lab Populat Genet, Moscow 119333, Russia.
Texas A&M Univ, Dept Ecosyst Sci & Management, College Stn, TX 77843 USA.

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Bondar, Eugeniya I.; Putintseva, Yuliya A.; Oreshkova, Nataliya V.; Krutovsky, Konstantin V.; Krutovsky, Konstantin; Government of the Russian Federation [14.Y26.31.0004]; University of Gottingen

/ N. V. Oreshkova, T. S. Sedel'nikova, S. P. Efremov, A. V. Pimenov // Contemp. Probl. Ecol. - 2020. - Vol. 13, Is. 6. - P569-576, DOI 10.1134/S1995425520060116. - Cited References:28 . - ISSN 1995-4255. - ISSN 1995-4263РУБ Ecology

Аннотация: The DNA polymorphism of seven coenopopulations of Siberian stone pine (Pinus sibirica Du Tour) growing in a region which is typical for mountain taiga pine forests-the northeastern part of the Kuznetsk Alatau-has been studied. According to the data of 11 nuclear microsatellite loci, 44 allelic variants which significantly differed between the studied coenopopulations in composition and frequency of occurrence are identified. The highest level of allelic diversity is found in the Ps_80612 and Ps_1502048 loci, in which eight and seven alleles are detected, respectively. The calculation of the main parameters of genetic diversity have shown, on the whole, a relatively low level of polymorphism of the studied samples (N-A = 3.078, N-E = 1.877, H-E = 0.445, and H-O = 0.401). An analysis of the degree of subdivision of coenopopulations has revealed that about 95% of the total genetic diversity is concentrated within populations, while the interpopulation component accounts for only about 5% (F-ST = 0.049). The diagnosed differences in the level of P. sibirica genetic polymorphism are determined by several factors: the presence of orographic and phytocenotic barriers between the coenopopulations, which define the directions of seed propagation vectors along the watercourses of river basins that form the Chulym River in the eastern sector of the Kuznetsk Alatau and Tom River in the western sector; a significant level of bioclimatic and environmental extremeness of habitats of coenopopulations in accordance with the height above sea level; and anthropogenic pressure, including periodic deforestation and recreational impact on coenopopulations located near settlements. These factors, which have a significant microevolutionary component, determine the adaptation trends of coenopopulations of P. sibirica and morphological singularities and reproductive potential of trees and, ultimately, they control their genetic polymorphism in the studied part of the Kuznetsk Alatau.

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Держатели документа:
Russian Acad Sci, Fed Res Ctr, Kola Sci Ctr, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Oreshkova, N., V; Sedel'nikova, T. S.; Efremov, S. P.; Pimenov, A., V