Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

[Text] / G. G. Polyakova, V. V. Stasova, N. V. Pashenova // Russ. J. Plant Physiol. - 2011. - Vol. 58, Is. 5. - P819-827, DOI 10.1134/S1021443711050177. - Cited References: 23. - This work was supported by the Russian Foundation for Basic Research, project no. 09-04-09030. . - 9. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: Ophiostomatoid fungi colonize the conducting tissues of conifer stems, the phloem and the xylem. These pathogenic fungi penetrate into the stem through injuries made by xylophagous insects vectoring these pathogens. In this study the response of the phloem of Scotch pine (Pinus sylvestris L.) to wounding (treatment 1) was compared with the response to wounding combined with application of high-molecular-weight compounds isolated from the mycelium of the ophiostomatoid fungus Ceratocystis laricicola Redfern & Minter (treatment 2). Both treatments induced the appearance of necrosis in the inner bark, the formation of periderm separating living and dead tissues, and formation of the callus alongside the wound perimeter. In addition, the bark accumulated lignin, bound proanthocyanidins, and resins, with a parallel decrease in the content of free proanthocyanidins, low-molecular-weight carbohydrates, and non-lignin components of the cell wall (P > 0.95). The size of necrotic spots, as well as changes in the content of most substances, were significantly higher in the treatment 2 than in the treatment 1 (P > 0.95). The accumulation of lignin in cell walls of phloem sieve cells was delayed in the treatment 2 as compared with that in the treatment 1. This suggested that the mycelial extract temporarily inhibited lignification at the early stage of the wound response. This disturbance of the cell wall protective transformation led to the hypothesis that the fungal suppressors retard the repair of inner bark injured by insects, thereby favoring the invasion of conifer tissues by ophiostomatoid fungi.

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Держатели документа:
[Polyakova, G. G.
Stasova, V. V.
Pashenova, N. V.] Russian Acad Sci, Sukachev Inst Forest, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Stasova, V.V.; Pashenova, N.V.

[Text] / G. G. Polyakova [et al.] // Russ. J. Plant Physiol. - 2008. - Vol. 55, Is. 4. - P496-502, DOI 10.1134/S1021443708040109. - Cited References: 25 . - 7. - ISSN 1021-4437РУБ Plant Sciences

Аннотация: The elicitor activity of compounds extracted from the mycelia of six species of phytopathogenic fungi was assessed from the sizes of necrotic lesions on the external surface of the living trunk phloem of five coniferous species inhabiting Siberia: Siberian larch (Larix sibirica L.), Scotch pine (Pinus sylvestris L.), Siberian spruce (Picea obovata Ledeb.), Siberian fir (Abies sibirica L.), and cedar pine (Pinus sibirica (Rupr.) Mayr.). The compounds for inoculation were extracted from the mycelium of ascomycetes imperfect, and basidium fungi; the living mycelia of these fungi were also used. The fungal extract or mycelium was placed into the hollows 7 mm in diameter in the trunk bark. Infection triggered the formation of hypersensitivity necrotic lesions in the inner bark exceeding in size those appeared after control wounding of four tree species (larch, pine, spruce, and cedar); fir was an exclusion. In experiments with tree trunks and conifer calluses, a dependence of immune response parameters (the sizes of necrotic lesions and the content of lignin and bound proanthocyanidins) on the quantity of the fungal preparation was elucidated. The largest necrotic lesions appeared after injection of 500 mu g of the fungal preparation into the hollow in the trunk, and its higher quantities did not increase the indices measured. The size of the necrotic lesion on the trunk bark is supposed to be used as a promising index characterizing the level of tree immunity and tolerance under various ecological conditions.

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Держатели документа:
[Polyakova, G. G.
Pashenova, N. V.
Polyakov, V. I.
Zrazhevskaya, G. K.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Div, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Polyakova, G.G.; Pashenova, N.V.; Polyakov, V.I.; Zrazhevskaya, G.K.