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    Development of new mitochondrial DNA markers in Scots pine (Pinus sylvestris L.) for population and phylogeographic studies
[Text] / V. L. Semerikov [et al.] // Russ. J. Genet. - 2015. - Vol. 51, Is. 12. - P1199-1203, DOI 10.1134/S1022795415120108. - Cited References:20. - We thank Y.Y. Hhrunyk, A. I. Vidjakin, V.V. Tarakanov, E.V. Hantemirova, and I.V. Tikhonova for assistance with the pine material collection. The study was supported by Russian Foundation for Basic Research (grant 13-04-01028) and by Russian Federation Government (grant 14.Y26.31.0004). . - ISSN 1022-7954. - ISSN 1608-3369
РУБ Genetics & Heredity

Аннотация: Fragments of genomic DNA of Scots pine (Pinus sylvestris L.) homologous to the mitochondrial DNA (mtDNA) contigs of Norway spruce (Picea abies (L.) Karst.) and loblolly pine (Pinus taeda L.) were resequenced in a sample of the Scots pine trees of European, Siberian, Mongolian, and Caucasian origin in order to develop mtDNA markers. Flanking non-coding regions of some mitochondrial genes were also investigated and resequenced. Five single nucleotide polymorphisms (SNPs) and a single minisatellite locus were identified. Caucasian samples differed from the rest by three SNPs. Two SNPs have been linked to an early described marker in the first intron of the nad7 gene, and all together revealed three haplotypes in European populations. No variable SNPs were found in the Siberian and Mongolian populations. The minisatellite locus contained 41 alleles across European, Siberian, and Mongolian populations, but, this locus demonstrated a weak population differentiation (F (ST) = 5.8), probably due to its high mutation rate.

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Держатели документа:
Russian Acad Sci, Ural Branch, Inst Plant & Anim Ecol, Ekaterinburg 620144, Russia.
Siberian Fed Univ, Genome Res & Educ Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Siberian Branch, Sukachev Inst Forest, Krasnoyarsk 660036, Russia.
Univ Gottingen, D-37077 Gottingen, Germany.
Russian Acad Sci, Vavilov Inst Gen Genet, Moscow 119991, Russia.
Texas A&M Univ, College Stn, TX 77843 USA.

Доп.точки доступа:
Semerikov, V. L.; Putintseva, Yu. A.; Oreshkova, N. V.; Semerikova, S. A.; Krutovsky, K. V.; Russian Foundation for Basic Research [13-04-01028]; Russian Federation Government [14.Y26.31.0004]
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